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Propagation of Ornamental Plants Vol. 5, 3, 2005: 151-157 THIDIAZURON INDUCES HIGH FREQUENCY SHOOT BUD FORMATION FROM  BEGONIA PETIOLE TRANSVERSE THIN CELL LAYER CULTURE Duong Tan Nhut 1* , Nguyen Thanh Hai 1 , Phan Xuan Huyen 1 , Doan Thi Quynh Huong 1 , Nguyen Thi Thuy Hang 1 and Jaime A. Teixeira da Silva 2 Department of Plant Biotechnology, Dalat Institute of Biology  6 Xo Viet Nghe Tinh, Dalat, Lam Dong, Vietnam, Tel.: +84 91 8313045, Fax: +84 63 831028, E-mail: [email protected] 2 Faculty of Agriculture, Kagawa University, Miki-cho, Ikenobe, 2393, Kagawa-ken, 761-0795, Japan REFERENCES Bui V. L., Hang Phuong N. T., Anh Hong L. T., Tran Thanh Van K. (1999). High frequency shoot regeneration from Rhynchostylis gigantea (Orchidaceae) using thin cell layers. Plant Growth Regulator, 28: 179–185. Bui V. L., Nghieng Thao D. M., Jeanneau M., Sadik S., Shanjun T., Vidal J., Tran Thanh Van K. (1998). Rapid  plant regeneration of a C 4 dicot species: Amaranthus edulis. Plant Science, 132: 45–54. Carimi F., De-Pasquale F., Crescimanno F. G. (1999). Somatic embryogenesis and plant regeneration from pistil thin cell layers of Citrus. Plant Cell Reports, 18: 935–940. Chin Y. L. (1993). The use of thidiazuron in tissue culture. In Vitro Cell and Development al Biology – Plant, 29: 92–96. Chlyah A., Tran Thanh Van K. (1968). Budding capacity of undetached  Begonia rex leaves. Nature, 218: 493. Chlyah A., Tran Thanh Van K. (1975). Different reactivity in epidermal cells of  Begonia rex excised and grown in vitro. Plant Physiology, 35: 16–20. Chlyah A., Tran Thanh Van K. (1984). Histo logical changes in epidermal and sub - epidermal cell laye rs of  Begonia rex induced to form de novo unicellular hairs, buds and roots. Botanical Gazette, 145: 55–59. Das A. B., Rout G. R., Das P. (1995). In vitro somatic embryogenesis from callus culture of the timber yielding tree Hardwickia binata Roxb. Plant Cell Reports, 15: 147–149. Duncan D. B. (1995). Multiple range and multiple F test. Biometrics, 11: 1–42. Eapen S., Tivarekar S., George L. (1998). Thidiazuron induced shoot regeneration in pigeonpea ( Cajanus cajan L.). Plant Cell, Tissue and Organ Culture, 53: 217–220. Gill R., Saxena P. K. (1992). Direct somatic embryogenesis and regeneration of plants from seedling explants of peanut (  Arachis hypogae L.): Promotive role of thidiazuron. Canadian Journal of Botany, 70: 1186–1192. Gray D. J., Benton C. M. (1991).  In vitro micropropagation and plant establishment of muscadine grape cultivars (V itis rotundifolia). Plant Cell, Tissue and Organ Culture, 27, 7–14. Heide O. M. (1964). Effects of light and temperature on the regeneration ability of  Begonia leaf cuttings. Plant Physiology, 17: 789–804. Huetteman C. A., Preece J. E. (1993). Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell, Tissue and Organ Culture, 33: 105–119. Inoue Y. (1983). Encyclopedia of Horticulture. 2B. Tokyo, Seibundo-Shinkosha. Juillien F., Tran Thanh Van K. (1994). Micropropagation and embryoid formation from young leaves of  Bambusa  glaucescens ‘Golden Goddess’. Plant Science, 98: 199–207. Juillien F., Wyndaele R. (1992). Precocious in vitro owering of soybean cotyledonary node. Journal of Plant Physiology, 140: 251–253. Maier U., Sattler R. (1977). The structure of the epiphyllous appendages Begonia hispida var. cucullifera. Cana- dian Journal of Botany, 55: 264–280. Malik K. A., Saxena P. K. (1992). Thidiazuron induces high-frequency shoot regeneration in intact seedlings of pea (  Pisum s ativum), chickpea (Cicer arietinum) and lentil (  Lens culinaris ). Australian Journal of Plant Physiology, 19: 731–740. Massimo H. M., Sanago V. I., Strommar J. (1996). Rapid plant regeneration of pea using thidiazuron. Plant Cell ,

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Propagation of Ornamental Plants

Vol. 5, № 3, 2005: 151-157

THIDIAZURON INDUCES HIGH FREQUENCY SHOOT BUD FORMATION FROM  BEGONIA 

PETIOLE TRANSVERSE THIN CELL LAYER CULTURE

Duong Tan Nhut1*, Nguyen Thanh Hai1, Phan Xuan Huyen1, Doan Thi Quynh Huong1,

Nguyen Thi Thuy Hang1 and Jaime A. Teixeira da Silva2

Department of Plant Biotechnology, Dalat Institute of Biology  6 Xo Viet Nghe Tinh, Dalat, Lam Dong, Vietnam,

Tel.: +84 91 8313045, Fax: +84 63 831028, E-mail: [email protected] Faculty of Agriculture, Kagawa University, Miki-cho, Ikenobe, 2393, Kagawa-ken, 761-0795, Japan

REFERENCES

Bui V. L., Hang Phuong N. T., Anh Hong L. T., Tran Thanh Van K. (1999). High frequency shoot regeneration

from Rhynchostylis gigantea (Orchidaceae) using thin cell layers. Plant Growth Regulator, 28: 179–185.

Bui V. L., Nghieng Thao D. M., Jeanneau M., Sadik S., Shanjun T., Vidal J., Tran Thanh Van K. (1998). Rapid

 plant regeneration of a C4

dicot species: Amaranthus edulis. Plant Science, 132: 45–54.

Carimi F., De-Pasquale F., Crescimanno F. G. (1999). Somatic embryogenesis and plant regeneration from pistil

thin cell layers of Citrus. Plant Cell Reports, 18: 935–940.

Chin Y. L. (1993). The use of thidiazuron in tissue culture. In Vitro Cell and Developmental Biology – Plant,

29: 92–96.

Chlyah A., Tran Thanh Van K. (1968). Budding capacity of undetached Begonia rex leaves. Nature, 218: 493.

Chlyah A., Tran Thanh Van K. (1975). Different reactivity in epidermal cells of  Begonia rex excised and grown

in vitro. Plant Physiology, 35: 16–20.

Chlyah A., Tran Thanh Van K. (1984). Histological changes in epidermal and sub - epidermal cell layers of  Begonia 

rex induced to form de novo unicellular hairs, buds and roots. Botanical Gazette, 145: 55–59.

Das A. B., Rout G. R., Das P. (1995). In vitro somatic embryogenesis from callus culture of the timber yielding

tree Hardwickia binata Roxb. Plant Cell Reports, 15: 147–149.

Duncan D. B. (1995). Multiple range and multiple F test. Biometrics, 11: 1–42.

Eapen S., Tivarekar S., George L. (1998). Thidiazuron induced shoot regeneration in pigeonpea (Cajanus cajan 

L.). Plant Cell, Tissue and Organ Culture, 53: 217–220.

Gill R., Saxena P. K. (1992). Direct somatic embryogenesis and regeneration of plants from seedling explants

of peanut ( Arachis hypogae L.): Promotive role of thidiazuron. Canadian Journal of Botany, 70: 1186–1192.

Gray D. J., Benton C. M. (1991). In vitro micropropagation and plant establishment of muscadine grape cultivars

(Vitis rotundifolia). Plant Cell, Tissue and Organ Culture, 27, 7–14.

Heide O. M. (1964). Effects of light and temperature on the regeneration ability of  Begonia leaf cuttings. Plant

Physiology, 17: 789–804.Huetteman C. A., Preece J. E. (1993). Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell,

Tissue and Organ Culture, 33: 105–119.

Inoue Y. (1983). Encyclopedia of Horticulture. 2B. Tokyo, Seibundo-Shinkosha.

Juillien F., Tran Thanh Van K. (1994). Micropropagation and embryoid formation from young leaves of  Bambusa

 glaucescens ‘Golden Goddess’. Plant Science, 98: 199–207.

Juillien F., Wyndaele R. (1992). Precocious in vitro owering of soybean cotyledonary node. Journal of Plant

Physiology, 140: 251–253.

Maier U., Sattler R. (1977). The structure of the epiphyllous appendages Begonia hispida var. cucullifera. Cana-

dian Journal of Botany, 55: 264–280.

Malik K. A., Saxena P. K. (1992). Thidiazuron induces high-frequency shoot regeneration in intact seedlings

of pea ( Pisum sativum), chickpea (Cicer arietinum) and lentil ( Lens culinaris). Australian Journal of Plant

Physiology, 19: 731–740.Massimo H. M., Sanago V. I., Strommar J. (1996). Rapid plant regeneration of pea using thidiazuron. Plant Cell,

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Tissue and Organ Culture, 45: 165–168.

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Tran Thanh Van M. (1973). In vitro control of de novo ower, bud, root and callus differentiation from excised

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Plant Cell, Tissue and Organ Culture, 36: 73–79.