Vol. 15 No. 7 July 2016 Editorial Board Contents Technical Papers · 2016-08-17 · Contents...

Vol. 15 No. 7 July 2016

ContentsTechnical Papers

• Numerical studies on the effect of various parameters on wrinkling in deep drawing of cylindrical cupsB V S Rao, G Chandra Mohan Reddy, G Krishna Mohan Rao and P V R Ravindra Reddy 3

• Evaluation of thrust force and surface roughness in drilling of Al-2219/B4C/Gr metal matrix composites

Suresh R 15

• Finite element analysis of metal removal rate on AL/SIC-MMC using EDM process

Sri Rama Krishna AA, Laxmaiah G and Siva Priya A 22

• A Brief Experimental Investigation on Wearing of CNT Reinforced Alumina Insert

Snehashis Sarkar, Krishnendu Mondal, Santanu Das and Probal Kumar Das 31

Select Bibliography: Sheet Metal Forming 28

Photo Gallery: Vintage Machine: Ten Inch Double Tier Bed Lathe 30

Select Bibliography of Standards: Sheet Metal Forming 36

Calendar of Events 37

Technology Trends 40

Manufacturing Technology Abstracts 42

Patent Abstracts: Sheet Metal Forming 52

IPR News: A - Z Definitions of Intellectual Property Terminology 57

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Editorial Board

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Disclaimer: Technical Data presented and views expressed by the authors are their own. CMTI does not assume any responsibility for the same.

ChairmanS Satish Kumar

Technical Paper Review PanelK K RajagopalB R MohanrajM ChellamalaiV ShanmugarajV G Yoganath

EditorMallikarjun G

Editorial AssistantsShashi Rekha NMala RC

Editorial Advisory CommitteeS UshaN BalashanmugamS Arumugasamy Dr. C K Srinivasa, Honorary Member

Bibliography

Manufacturing Technology Today, July 201628

SELECT BIBLIoGRAPhy: ShEET METAL FoRMING

1. Development of experimental and theoretical forming limit diagrams for warm forming of austenitic stainless steel 316 Hussaini, Syed Mujahed; Krishna, Geetha; Gupta, Amit Kumar; Singh, Swadesh Kumar [J of Manufacturing Processes, V 18, 2015, Starting page 151, 8 Pages] Rec. No: 110535

2. Analytical and experimental investigations on deformation mechanism and fracture behavior in single point incremental forming Fang, Y; Lu, B; Chen, J; Xu, DK; Ou, H [J of Materials Processing Technology, V 214, N 8, 2014, Starting page 1503, 8 Pages] Rec. No: 110465

3. 3D forming tool optimisation method considering springback and thinning compensation

Mole, N; Cafuta, G; Štok, B [J of Materials Processing Technology, V 214, N 8, 2014, Starting page 1673, 13 Pages] Rec. No: 110467

4. Surface flexible rolling for three-dimensional sheet metal parts

Li, Ren-Jun; Li, Ming-Zhe; Qiu, Ning-Jia, Cai, Zhong-Yi [J of Materials Processing Technology, V 214, N 2, 2014, Starting page 380, 10 Pages] Rec. No: 110029

5. Experimental and numerical investigation on micro deep drawing process of stainless steel 304 foil using flexible tools

Irthiea, Ihsan; Green, Graham; Hashim, Safa; Kriama, Abdulbast [Int J of Machine Tools & Manufacture, V 76, 2014, Starting page 21, 13 Pages] Rec. No: 110027

6. Effect of material scatter on the plastic behavior and stretchability in sheet metal forming

Wiebenga, JH; Atzema, EH; An, YG; Vegter, H; van den Boogaard, AH [J of Materials Processing

Technology, V 214, N 2, 2014, Starting page 238, 15 Pages] Rec. No: 110031

7. Local heating method by near-infrared rays for forming of non-quenchable advanced high-strength steels

Lee, Eun-Ho; Hwang, June-Sun; Lee, Chang-Whan; Yang, Dong-Yol; Yang, Woo-Ho [J of Materials Processing Technology, V 214, N 4, 2014, Starting page 784, 10 Pages] Rec. No: 110288

8. Finite element modeling and experimental results of brass elliptic cups using a new deep drawing process through conical dies

Dhaiban, Abdullah A; Soliman, M.-Emad S; El-Sebaie, MG [J of Materials Processing Technology, V 214, N 4, 2014, Starting page 828, 11 Pages] Rec. No: 110286

9. Formability limits by fracture in sheet metal forming

Isik, K; Silva, MB; Tekkaya, AE; Martins, PAF [J of Materials Processing Technology, V 214, N 8, 2014, Starting page 1557, 9 Pages] Rec. No: 110569

10.Development of a biaxial loading frame for sheet metal

Wilson, Joseph F; Kinsey, Brad L; Korkolis, Yannis P [J of Manufacturing Processes, V 15, N 4, 2013, Starting page 580, 6 Pages] Rec. No: 109740

11.Development of a new procedure for the experimental determination of the forming limit curves

Banabic, D; Lazarescu, L; Paraianu, L; Ciobanu, I; Nicodim, I; Comsa, DS [CIRP Annals, V 62, N 1, 2013, Starting page 255, 4 Pages] Rec. No: 109279

12.An isotropic plasticity model coupled with strain dependent plastic strain & stress ratios

Sheet metal forming processes are those in which force is applied to a piece of sheet metal to modify its geometry rather than remove any material. The applied force stresses the metal beyond its yield strength, causing the material to plastically deform, but not to fail. By doing so, the sheet can be bent or stretched into a variety of complex shapes. Sheet metal forming processes include Bending, Roll forming, Spinning, Deep Drawing, Stretch forming

Bibliography

Manufacturing Technology Today, July 2016 29

Volk, Wolfram; Kim, Jae Kun; Suh, Joungsik; Hartmut, Hoffmann [CIRP Annals, V 62, N 1, 2013, Starting page 283, 4 Pages] Rec. No: 109376

13.Methodology for off-line evaluation of new environmentally friendly tribo-systems for sheet metal forming

Ceron, Ermanno; Bay, Niels [CIRP Annals, V 62, N 1, 2013, Starting page 231, 4 Pages] Rec. No: 109419

14.New method for predicting Forming Limit Curves from mechanical properties

Abspoel, Michael; Scholting, Marc E; Droog, John MM [J of Materials Processing Technology, V 213, N 5, 2013, Starting page 759, 11 Pages] Rec. No: 109798

15.Cruciform shape benefits for experimental and numerical evaluation of sheet metal formability

Lionel Leotoing; Dominique Guines; Ibrahim Zidane; Eric Ragneau [J of Materials Processing Technology, V 213, N 6, 2013, Starting page 856, 8 Pages] Rec. No: 109853

16.An experimental and numerical investigation on polymer melt injected sheet metal forming.

Hussain, Muhammad Masood; Trompeter, Michael; Witulski, Jörg; Tekkaya, A Erman [J of Manufacturing Sci & Engg: ASME Trans, V 134, N 3, 2012, Starting page 031005, 13 Pages] Rec. No: 108706

17. Investigation of strain gradients and magnitudes during microbending

Schultz, Jesse; Griese, David; Ju, Jaehyung; Shankar, Prabhu; Summers, Joshua D; Thompson, Lonny [J of Manufacturing Sci & Engg: ASME Trans, V 134, N 4, 2012, Starting page 041011, 9 Pages] Rec. No: 108848

18.Failure prediction for nonlinear strain paths in sheet metal forming

Volk, Wolfram; Hoffmann, Hartmut; Suh, Joungsik; Kim, Jaekun [CIRP Annals, V 61, N 1, 2012, Starting page 259, 4 Pages] Rec. No: 109099

19.Balancing procedure for energy and material flows in sheet metal forming.

Goschel, A ; Sterzing, A ; Schonherr, J [CIRP J of Manufacturing Science & Technology, V 4, N 2, 2011, Starting page 170, 10 Pages] Rec. No: 108213

20.hot sheet metal forming: the formulation of graded component characteristics based on strategic temperatures management for tool-based and incremental forming operations.

Neugebaucer, R ; Schieck, F ; Rautenstrauch,

A; Bach, M [CIRP J of Manufacturing Science & Technology, V 4, N 2, 2011, Starting page 180, 9 Pages] Rec. No: 108192

21.Tooling System Design for Forming Aluminum Beverage Can End Shells

Yamazaki, Koetsu; Han, Jing; Otsuka, Takayasu; Hasegawa, Takashi; Nishiyama, Sadao [J of Mechanical Design: ASME Trans, V 133, N 11, 2011, Starting page 114502, 6 Pages] Rec. No: 108488

22. Investigation into wall wrinkling in deep drawing process of conical cups

Shafaat, Mohammad Amin; Mahmoud, Abbasi; Ketabchi, Mostafa [J of Materials Processing Technology, V 211, N 11, 2011, Starting page 1783, 13 Pages] Rec. No: 108497

23. Impact of superimposed pressure on dieless incremental sheet metal forming with two moving tools.

Meier, H; Magnus, C; Smukala, V [CIRP Annals, V 60/1, 2011, Starting page 327, 4 Pages] Rec. No: 108503

24.Enhancement of bending formability of brittle sheet metal in multilayer metallic sheets.

Yanagimoto, J; Oya, T; Kawanishi, S; Tiesler, N; Koseki, T [CIRP Annals, V 59, N 1, 2010, Starting page 287, 4 Pages] Rec. No: 105909

25.Tailor die quenching in hot stamping for producing ultra-high strength steel formed parts having strength distribution.

Mori, K ; Okuda, Y [CIRP Annals, V 59, N 1, 2010, Starting page 291, 4 Pages] Rec. No: 105914

26.Time dependent determination of forming limit diagrams.

Merklein, M; Kuppert, A; Geiger, M [CIRP Annals, V 59, N 1, 2010, Starting page 295, 4 Pages] Rec. No: 105915

27.optimization method for stamping tools under reliability constraints using genetic algorithms and finite element simulations.

Ledoux, Y; Sebastian, P; Samper, S [J of Materials Processing Tech, V 210, N 3, 2010, Starting page 474, 13 Pages] Rec. No: 106539

28.Environmentally benign tribo-systems for metal forming.

Bay, N; Azushima, A; Groche, P; Ishibashi, I; Merklein, M; Morishita, M; Nakamura, T; Schmid, S; Yoshida, M [CIRP Annals, V 59, N 2, 2010, Starting page 760, 21 Pages] Rec. No: 105729 ◘

Events


NATIoNAL

10 - 12 Aug 2016TechIndia New Delhi: International Exhibition on Engineering and ManufacturingPragati Maidan Exhibition Center,Mathura Road, 110002 New DelhiContact: International Trade and Exhibitions India Pvt. Ltd.11th Floor, Kailash Building Kasturba Gandhi Marg110001 New DelhiEmail: [email protected] Web: itei.in

10 - 12 Aug 2016MMMM 2016Pragati Maidan, New DelhiContact: Gagan SahniDirector Sales and OperationsPh: +91 9810036183E: [email protected] Trade and Exhibitions India Pvt. Ltd., B 309, 3rd Floor, Statesman House, 29, Barakhambha Road, New Delhi - 110001Tel: +91 9810036183 Web: www.mmmm-expo.com

22 - 25 Aug 2016Automation Mumbai: International exhibition and conference for automationBombay Convention & Exhibition Centre BCEC, Western Express Highway, 400063 Mumbai, MaharashtraContact: IED Communications Ltd., 64, Empire Building D N Road, Fort, 400001 Mumbai, IndiaTel: +91 (0)22 22079567 Email: [email protected] Web: www.iedcommunications.com

21 - 23 Sep 2016Laser World of Photonics India BangaloreBIEC - Bangalore International Exhibition Center,10th Mile Tumku Road, 562123 Bangalore, KarnatakaContact: MMI India Pvt. Ltd.5th Floor, Cardinal Gracias Road400 052 Mumbai, IndiaTel: +91 (2)2 42554700 Fax: +91 (2)2 42554719Email: [email protected] Web: www.mmi-india.in

22 - 24 Sep 2016International exhibition on packaging material and technologyPragati Maidan Exhibition Center,Mathura Road, 110002 New DelhiContact: Koelnmesse YA Tradefair Pvt. Ltd.Office 1102, 11th Floor DLH Park, S. V. Road400067 Mumbai, IndiaTel: +91 (0)22 28715200 Fax: +91 (0)22 28715222Email: [email protected] Web: www.koelnmesse-india.com

29 Sep - 02 oct 2016Machine Tool Expo 2016 –PuneAuto Cluster Exhibition, Centre, PuneContact: Mahesh, T: +91 (0) 9886680466E: [email protected] International Exhibition Centre (BIEC)10th Mile, Tumkur Road, Madavara Post, Bangalore - 562 123, Karnataka Tel: +91 80 6624 6600 Fax: +91 80 6624 6661Web: [email protected]

05 - 07 oct 2016Metallurgy India: 6th International Exhibition on Metallurgical Technology, Processes, Material handling and Services 2016Bombay Convention & Exhibition Centre Western Express Highway, NESCO, Goregaon East, Mumbai, Maharashtra 400063Contact: Messe Duesseldorf, Stockumer Kirchstrasse 61D-40474 DüsseldorfTel: +49 - 211 - 4560 900 Fax: +49 - 211 - 4560 668Email: [email protected] Web: http://eventegg.com/metallurgy-india/

06 - 07 oct 20166th International Conference on ‘Additive Manufacturing Technologies-AM 2016Hotel Lalit Ashok, BengaluruContact: Additive Manufacturing Society of India (AMSI)#42, BSK 3rd Stage, 3rd Phase, 3rd Block9th Cross, Bangalore-560 085 Tel: +91 9483717449 Fax: +91 80 26729437/ 32422663Email: [email protected] Web: http://amsi.org.in

Events


INTERNATIoNAL 21 oct 2016Smart Manufacturing SummitHotel Le Meridien, New Delhi, IndiaContact: Confederation of Indian Industry, Lodi Road, New Delhi-110003, VisakhapatnamWeb: http://10times.com

07 - 11 Nov 2016Intl Conf on Technologically Advanced Materials & Asian Meeting on Ferroelectricity (ICTAM-AMF10)Conference Center, University of Delhi, DelhiContact: Prof. R. P. Tandon, General Chair, ICTAM-AMF10 Tel:+91-9818229608Email: [email protected] Web: www.amf2016.in

11 - 13 Nov 2016umex Chennai: Trade fair for used machineryChennai Trade Center, Poonamali High Road, 600010 Chennai, Tamil NaduContact: ITEI International Trade and Exhibitions India Pvt. Ltd., E519 Floral Deck Plaza, Central MIDC Road, Opp SEEPZ, 400093 MumbaiTel: +91 (0)22 28398000 Fax: +91 (0)22 28390502Email: [email protected] Web: www.itei.in

23 - 26 Nov 2016Rajkot Machine tools show -2016NSIC Ground, Aji Industrial Area, Rajkot, GujaratContact: KMG Business Technology & Machine Tools MFRS Association-RajkotWeb: tradeshows.tradeindia.com

29 Nov 2016Edition of Manufacturing SummitTrident Hotel, MumbaiContact: Confederation of Indian IndustryLodi Road, New Delhi-110003Visakhapatnam, IndiaWeb: http://www.eventseye.com

01 - 03 Dec 2016Surface Technology India MumbaiBandra Kurla Complex, Mumbai, MaharashtraContact: Hannover Milano Fairs India Pvt Ltd., No 13/16, Vatika Business Centre Wing B, 400076 MumbaiTel: +91 (0)22 42369710 Fax: +91 (0)22 42019191Email: [email protected] Web: www.hmf-india.com

03 - 06 Aug 2016Manufacturing Surabaya:International trade fair for machinery, equipment, materials and services for the manufacturing industryGrand City Expo, Jl Gubeng Pojok Dalam Surabaya, Surabaya, East Java, Java, IndonesiaContact: PT Pamerindo IndonesiaDeutsche Bank Building, 13th Floor, Jl. Imam Bonjol 80, 10310 Jakarta, IndonesiaTel: +62 (0)21 3162001 Fax: +62 (0)21 31619812Email: [email protected] Web: www.pamerindo.com

26 - 29 Aug 2016International fair of the manufacturing industryDiamond Island Convention and Exhibition Center,Diamond Island opposite the Hun Sen Park, Phnom Penh, Phnom Penh, CambodiaContact: Chan Chao International Co., Ltd.3F, No.185, Kangchien Road, 10041 Taipei, TaiwanTel: +886 (2) 26596000 Fax: +886 (2) 26597000Email: [email protected] Web: www.chanchao.com.tw

28 Sep 2016Exhibition of products of the process control, instrumentation, control and control technologyFriedrich–Ebert-Halle, Erzbergerstr. 89, 67063 Ludwigshafen, Rhineland-Palatinate, GermanyContact: Meorga GmbH, Sportplatzstr. 2766809 Nalbach, GermanyTel: +49 (0)6838 8960035 Fax: +49 (0)6838 983292Email: [email protected] Web: www.meorga.de

29 Sep - 01 oct 2016Trade fair for the professional metallurgy industryIstanbul Fuar Merkez - IFM,IFM Istanbul Expo Center, Istanbul, Istanbul, TurkeyContact: Hannover-Messe Ankiros Fuarcilik A.S.Abdullah Cevdet Sok 6/2, 06680 Ankara, TurkeyTel: +90 (0)312 4396792 Fax: +90 (0)312 4396766Email: [email protected] Web: www.ankiros.com

Events


04 - 06 oct 2016Trade fair for machine tools, tools and process technologyExpo Silesia, Braci Mieroszewskich 124, 41219 Sosnowiec, Silesia, PolandContact: Expo Silesiaul. Braci Mieroszewskich 12441-219 Sosnowiec, PolandTel: +48 (0)32 7887500 Fax: +48 (0)32 7887501Email: [email protected] Web: www.exposilesia.pl

05 - 07 oct 2016Trade fair for mechanical components and materials technologyIntex Osaka International Exhibition Center,1-5-102, Nanko-kita, Suminoe-ku, 531-6035 Osaka, Osaka, JapanContact: Reed Exhibitions Japan Ltd.1-26-2 Nishishinjuku163-0570 Tokyo, JapanTel: + 81 (3) 33498501 Fax: + 81 (3) 33498599Email: [email protected] Web: www.reedexpo.co.jp

10 - 13 oct 2016testXpo ulm: Forum for materials testingZwick GmbH & Co. KG, August-Nagel-Str. 11, 89079 Ulm, Baden-Wurttemberg, GermanyContact: Zwick GmbH & Co. KG, August-Nagel-Str. 1189079 Ulm, GermanyTel: +49 (0)7305 10-0 Fax: +49 (0)7305 10-200Email: [email protected] Web: www.zwick.de

10 - 13 oct 2016World's leading trade fair for automation in production and assemblyMesse Stuttgart, Messepiazza 1, 70629 Stuttgart, Baden-Wurttemberg, GermanyContact: Messe Sinsheim GmbHNeulandstr. 2774889 Sinsheim, GermanyTel: +49 (0)7261 6890 Fax: +49 (0)7261 689220Email: [email protected] Web: www.messe-sinsheim.de

13 - 15 oct 2016AMB China Nanjing: Machine tools exhibitionNanjing International Expo Center,Yanshan Road, 210019 Nanjing, Jiangsu, ChinaContact: Messe Stuttgart China Ltd.

Room 13A, Huadu Mansion, 838 Zhangyang Road, Pudong, 200122 Shanghai, ChinaTel: +86 (0)21 50811929 Fax: +86 (0)21 50813069Email: [email protected] Web: www.messe-stuttgart.com.cn

25 - 27 oct 2016International metal and steel trade fair for Southeast AsiaJakarta International Expo, Arena PRJ Kemayoran, 14410 Jakarta, Jakarta, Java, IndonesiaContact: Messe Düsseldorf Asia Pte Ltd3 HarbourFront Place099254 Singapore, SingaporeTel: +65 63329620 Fax: +65 63329655Email: [email protected] Web: mda.messe-dusseldorf.com

25 - 29 oct 2016International technology exhibition for sheet metal workingMesse Hannover, Messegelände, 30521 Hanover, Lower Saxony, GermanyContact: Mack Brooks Exhibitions Ltd.Romeland House, Romeland HillAL3 4ET St Albans, United Kingdom of Great Britain and Northern IrelandTel: +44 (0)1727 814400 Fax: +44 (0)1727 814401Email: [email protected] Web: www.mackbrooks.com

29 - 31 oct 20165th International Conference on Material Science and Engineering Technology (ICMSET 2016)Postal Code 113-8656 7-3-1 Hongo, Bunkyo-ku, TokyoContact: University of Tokyo, Japan Tel: +86-13467777771 Email: [email protected] Web: www.icmset.com

08 - 11 Nov 2016Metal Expo Moscow: International industrial exhibitionAll-Russian Exhibition Center (VVC), Estate 119, 129223 Moscow, Moscow, Russian FederationContact: Expo-Park Exhibition, 165, 10, Krymsky val119049 Moscow, Russian FederationTel: +7 (4)95 6579922 Fax: +7 (4)95 6057210Email: [email protected] Web: www.expopark.ru ◘

Abstracts


Manufacturing technology abstracts

MATERIALS & TREATMENT 43

PRoDuCT DESIGN & MANuFACTuRE 43

TooLS & TooLING 44

BIoSENSoRS 45

The topics on various aspects of manufacturing technology can be discussed in term of concepts, state of the art, research, standards, implementations, running experiments, applications, and industrial case studies.

Authors from both research and industry contributions are invited to submit complete unpublished papers, which are not under review in any other conference or journal.

Contact: Central Manufacturing Technology Institute (CMTI) Tumkur Road, Bengaluru – 560 022, Karnataka, IndiaEmail: [email protected]

Abstracts


Manufacturing technology abstracts

MATERIALS & TREATMENT

110756 In-situ infection detection sensor coating for urinary cathetersScarlet Milo, Naing Tun Thet, Dan Liu, Jonathan Nzakizwanayo, Brian V. Jones, A. Toby A. Jenkins [Biosensors & Bioelectronics, v 81, Jul 2016, Starting Page 166, Pages 7] We describe a novel infection-responsive coating for urinary catheters that provides a clear visual early warning of Proteus mirabilis infection and subsequent blockage. The crystalline biofilms of P. mirabilis can cause serious complications for patients undergoing long-term bladder catheterisation. Healthy urine is around pH 6, bacterial urease increases urine pH leading to the precipitation of calcium and magnesium deposits from the urine, resulting in dense crystalline biofilms on the catheter surface that blocks urine flow. The coating is a dual layered system in which the lower poly(vinyl alcohol) layer contains the self-quenching dye carboxyfluorescein. This is capped by an upper layer of the pH responsive polymer poly(methyl methacrylate-co-methacrylic acid) (Eudragit S100®). Elevation of urinary pH (>pH 7) dissolves the Eudragit layer, releasing the dye to provide a clear visual warning of impending blockage. Evaluation of prototype coatings using a clinically relevant in vitro bladder model system demonstrated that coatings provide up to 12 h advanced warning of blockage, and are stable both in the absence of infection, and in the presence of species that do not cause catheter blockage. At the present time, there are no effective methods to control these infections or provide warning of impending catheter blockage. (37 refs, 4 figs) (AA)

PRoDuCT DESIGN & MANuFACTuRE

110757 Method for analysing conceptual design process of product-service systemsShimomura, Yoshiki; Nemoto, Yutaro; Kimita, Koji [CIRP Annals, v 64, n 1, 2015, Starting Page 145, Pages 4] One of the major features of product-service systems (PSS) design is a variety of design elements. This makes its design process complex and obscure. This study aims to develop a method for analysing how the design process influences the features of design solutions in the

conceptual design of PSS. In this study, protocol analysis is applied to plural PSS design sessions. Simultaneously, their design solutions are evaluated relatively based on several aspects. Their design processes are analysed in connection with the above evaluation results. The findings obtained by using the proposed method will contribute to creating practical design guidelines for superior PSS development. (10 refs, 4 figs, 2 tables) (AA)

110758 Introducing life cycle thinking in product development – A case from Siemens Wind PowerBonou, Alexandra; Olsen, StigI.; Hauschild, Michael Z [CIRP Annals, v 64, n 1, 2015, Starting Page 45, Pages 4] How can use of LCA improve the environmental sustainability of wind industry products? An analysis of a case study from Siemens Wind Power identifies the knowledge offered by LCA that is relevant to each step of the product development process (PDP). The study illustrates the difference that this knowledge can make to the decision making in the PDP and to the environmental sustainability of the product. Based on these findings, the study concludes with a discussion of barriers for LCA integration in the PDP of complex products and possible measures to overcome them. (21 refs, 2 figs, 1 table) (AA)

110759 knowledge synthesis by least commitment for product design

Roucoules, Lionel; Tichkiewitch, Serge [CIRP Annals, v 64, n 1, 2015, Starting Page 141, Pages 4] Engineering design is a model-based activity which describes the

product’s multiple perspectives. With process model, we can also lead concurrent engineering activities. Both product and processmodels have been largely investigated. This paper aims at describing knowledge-based heterogeneous models chained to trace the design rationale that is the fundamental requirement to afford changes management. This knowledge chain supports the progressive by least commitment convergence of the space of design solutions. This rationale allows designers to go back and forth in the decision-making process. Moreover, the progressive

Abstracts


convergence increases the possibilities for designers to integrate new knowledge towards innovation. (16 refs, 6 figs) (AA)

TooLS & TooLING

110760 Parametric toolpath design in metal spinningPolyblank, James A; Allwood, Julian M [CIRP Annals, v 64, n 1, 2015, Starting Page 301, Pages 4] Toolpaths in metal spinning are still designed by human operators, largely by intuition: a scientific basis remains elusive. In this paper, a parameterised toolpath is proposed based on a quadratic Bezier curve. Experiments are performed varying each of four design parameters in turn, to investigate how tool force, part geometry and various failure modes evolve with key features of the tool path. Analysis of these experimental results reveals some new features of process mechanics and leads to a proposal for a set of rules that may become useful for automatic toolpath generation. (12 refs, 6 figs) (AA)

110761 Investigations on the thermal workpiece distortion in MQL deep hole drilling of an aluminium cast alloy

Biermann, D; Iovkov, I [CIRP Annals, v 64, n 1, 2015, Starting Page 85, Pages 4] Dry machining is

frequently applied in cutting operations, in order to reduce the energy consumption and the production costs. In deep hole drilling operations minimum quantity lubrication (MQL) is used to obtain a reliable chip evacuation, since completely dry machining is not feasible. Due to the low cooling effect of MQL, the drilling process generates a high thermal load on the workpiece, which leads to thermally induced workpiece deformations. This paper presents fundamental experimental investigations on the workpiece temperature, the resulting in-process deformations and the achievable straightness accuracy of the borehole. The investigations focus on two different strategies for enhancing the deep hole drilling using MQL. Initially, a high-feed process guiding is introduced, in order to obtain a higher productivity and to reduce the heat input into the workpiece. The second approach is a novel radial spindle compensation, which performs a directional

control of the straightness deviation of the deep hole. (16 refs, 9 figs) (AA)

110762 Cutting temperature measurement by a micro-sensor array integrated on the rake face of a cutting tool

Sugita, Naohiko; K e i g o I s h i i , Furusho, Tatsuo; Harada, Kanako; M i t s u i s h i , Mamoru [CIRP Annals, v 64, n 1,

2015, Starting Page 77, Pages 4] There is an ongoing demand for temperature measurement during machining processes; however, accomplishing this often proves to be a major challenge. In this paper, we propose a micro temperature sensor that is integrated on the rake face of a cutting tool. Specifically, we propose an array of micro thermocouples, which is implemented on an insert. After calibration, some experiments were conducted with an MC nylon workpiece. The performance of the proposed sensor was evaluated from the obtained thermo-electromotive force and the temperature measurements taken at multiple points. (12 refs, 9 figs, 3 tables) (AA)

110763 Prediction of part machining cycle times via virtual CNC

Altintas, Y; Tulsyan, S [CIRP Annals, v 64, n 1, 2015, Starting Page 361, Pages 4] This paper presents the virtual prediction of part machining

cycle times within 95% accuracy by considering the trajectory generation and corner smoothing models of commercial CNCs. The key functions of the CNC which control the machine motions are the real time interpolation, trajectory generation and feed drive control modules. It is shown that only the trajectory generation and the interpolation of tool path geometry are crucial in predicting the partmachining cycle times, and the servo control loops contribute negligible time delay. The proposed model is experimentally validated in machining 3 and 5-axis parts on commercial CNC machine tools. (10 refs, 7 figs, 1 table) (AA)

Abstracts


110764 high speed cornering strategy with confined contour error and vibration suppression for CNC machine tools

Sencer, Burak; Ishizaki, Kosuke; Shamoto, Eiji [CIRP Annals, v 64, n 1, 2015, Starting Page 369, Pages 4] This

paper presents a novel real-time trajectory generation algorithm for accurate high-speed cornering applications. Typically, reference tool-paths compromised of G01 lines are geometrically smoothed by means of arcs and splines. In this study, a kinematic corner smoothing algorithm approach is proposed where the cornering trajectory of the tool is generated through FIR (Finite Impulse Response) filtering of discontinuous axis velocity commands at segment junctions. Contouring errors at sharp corners are controlled analytically by optimally overlapping acceleration profiles of previous and present segments. Residual vibrations due to excitation of structural modes are avoided by tuning filter delays for all drives. The proposed method has been experimentally demonstrated to show significant improvement in the cycle time and accuracy of contouring Cartesian tool-paths. (8 refs, 6 figs) (AA)

BIoSENSoRS

110765 Label-free DNA biosensor based on resistance change of platinum nanoparticles assemblies

Evangelos Skotadis, Konstantinos Voutyras, Marianneza Chatzipetrou, Georgios Tsekenis, Lampros Patsiouras, Leonidas Madianos and others [Biosensors & Bioelectronics, v 81, Jul 2016, Starting Page

388, Pages 7] A novel nanoparticle based biosensor for the fast and simple detection of DNA hybridization events is presented. The sensor utilizes hybridized DNA’s charge transport properties, combining them with metallic nanoparticle networks that act as nano-gapped electrodes. The DNA hybridization events can be detected by a significant reduction in the sensor’s resistance due to the conductive bridging offered by hybridized DNA. By modifying the nanoparticle surface coverage, which can be

controlled experimentally being a function of deposition time, and the structural properties of the electrodes, an optimized biosensor for the in situ detection of DNA hybridization events is ultimately fabricated. The fabricated biosensor exhibits a wide response range, covering four orders of magnitude, a limit of detection of 1 nM and can detect a single base pair mismatch between probe and complementary DNA. (43 refs, 5 figs) (AA)

110766 Selective nitrate detection by an enzymatic sensor based on an extended-gate type organic field-effect transistor

Tsuyoshi Minami, Yui Sasaki, Tsukuru Minamiki, Shin-ichi Wakida, Ryoji Kurita, Osamu Niwa, Shizuo Tokito [Biosensors & Bioelectronics, v 81,

Jul 2016, Starting Page 87, Pages 5] First selective nitrate biosensor device based on an extended-gate type organic field-effect transistor (OFET) is reported. The fabricated sensor device consists of the extended-gate electrode functionalized by a nitrate reductase with a mediator (=a bipyridinium derivative) and an OFET-based transducer. The mechanism of the nitrate detection can be explained by an electron-relay on the extended-gate electrode, resulting in changes of the electric properties of the OFET. The detection limit of nitrate in water is estimated to be 45 ppb, which suggests that the sensitivity of our fabricated sensor is comparable to those of some conventional detection methods. As a practical application of the OFET sensor, the nitrate detection in diluted human saliva has been successfully demonstrated; the results agreed well with those by conventional colorimetric measurement. The advantages of OFETs are printability, mechanical flexibility, stretchability and disposability, meaning that the fabricated OFET could open up a new approach for low-cost electronic devices toward on-site detection of nitrate in aqueous media. (47 refs, 3 figs) (AA)

110767 Dopaminergic receptor–ligand binding assays based on molecularly imprinted polymers on quartz crystal microbalance sensorsWanpen Naklua, Roongnapa Suedee, Peter A. Lieberzeit [Biosensors & Bioelectronics, v 81, Jul 2016, Starting Page 117, Pages 8] Molecularly imprinted polymers (MIPs) have been successfully

Abstracts


applied as s e l e c t i v e materials for assessing the b i n d i n g activity of agonist and antagonist of dopamine D1

receptor (D1R) by using quartz crystal microbalance (QCM). In this study, D1R derived from rat hypothalamus was used as a template and thus self-organized on stamps. Those were pressed into an oligomer film consisting of acrylic acid: N-vinylpyrrolidone: N,N′-(1,2-dihydroxyethylene) bis-acrylamide in a ratio of 2:3:12 spin coated onto a dual electrode QCM. Such we obtained one D1R-MIP-QCM electrode, whereas the other electrode carried the non-imprinted control polymer (NIP) that had remained untreated. Successful imprinting of D1R was confirmed by AFM. The polymer can re-incorporate D1R leading to frequency responses of 100–1200 Hz in a concentration range of 5.9–47.2 µM. In a further step such frequency changes proved inherently useful for examining the binding properties of test ligands to D1R. The resulting mass-sensitive measurements revealed Kd of dopamine∙HCl, haloperidol, and (+)-SCH23390 at 0.874, 25.6, and 0.004 nM, respectively. These results correlate well with the values determined in radio ligand binding assays. Our experiments revealed that D1R-MIP sensors are useful for estimating the strength of ligand binding to the active single site. Therefore, we have developed a biomimetic surface imprinting strategy for QCM studies of D1R-ligand binding and presented a new method to ligand binding assay for D1R. (50 refs, 6 figs, 2 tables) (AA)

110768 Development of a label-free SPR sensor for detection of matrix metalloproteinase-9 by antibody immobilization on carboxymethyldextran chip

Sara Mohsen,Tahereh Tohidi Moghadam, Bahareh Dabirmanesh, Safoura Jabbari, Khosro Khajeh [Biosensors & Bioelectronics, v 81, Jul 2016, Starting Page 510, Pages 7] Surface plasmon resonance (SPR) immunosensor

has been widely utilized for monitoring antigen-antibody interactions. The sensor measures changes of refractive index upon binding of analyte molecules to specific ligand immobilized on the sensor chip. This effort reports development of SPR immunosensor for real-time and label-free detection of recombinant human matrix metalloproteinases-9 (MMP-9), which has been associated with malignant tumor progression and metastasis by matrix degradation. MMP-9 was expressed in Escherichia coli BL21 and purified by Ni-NTA agarose column. CMD 50 D was activated by EDC/NHS for immobilization of monoclonal anti-MMP-9. Atomic force microscopy images showed uniform distribution of anti-MMP-9 over the sensor chip. Equilibrium constant (KD), maximum binding capacity (Rmax) and ∆Gb values for interaction of MMP-9 and anti-MMP-9 were 0.4 nM, 680 µRIU and −53.51 kJ/mol, respectively. Concentration of MMP-9 in saliva samples was determined, with linearity in the range of 10–200 ng/mL. The limit of detection was found to be 8 pg/mL, being lower than most of the previously reported techniques. (48 refs, 4 figs, 1 table) (AA)

110769 highly sensitive and selective impedimetric aptasensor for interleukin-17 receptor A

Hunho Jo, S e o n g -Kyeong Kim, H y u n g j u n Y o u n , Heehyun Lee, Kwa n g hy u n Lee, Jian

Jeong, Jihyun Mok, Seung-Hyun Kim, Hae-Sim Park, Changill Ban, [Biosensors & Bioelectronics, v 81, Jul 2016, Starting Page 80, Pages 7] Interleukin-17 receptor A (IL-17RA) has been recognized as a valuable biomarker for diverse diseases, including autoimmune diseases. In this work, an electrochemical biosensor with great sensitivity and selectivity toward IL-17RA was fabricated using an IL-17RA aptamer (Kd=14.00 nM) for the first time. The aptasensor was manufactured using electrodeposition of gold nanoparticles, and then quantitative detection of IL-17RA was performed based on impedimetry. The developed sensor exhibited a superior analytical performance for IL-17RA with a wide dynamic range of 10–10,000 pg/mL in buffer and a detection limit of 2.13 pg/mL, which is lower than that of commercially available ELISA kits. In

Abstracts


addition, we validated the high specificity of the designed aptasensor to only IL-17RA, which showed good sensitivity even in human serum solution. Furthermore, the detection of the differentiated HL-60 cells expressing IL-17RA was successfully performed. Clinical applicability of the sensor was also demonstrated utilizing neutrophils separated from asthma patients. It is expected that the fabricated aptasensor will become an excellent diagnostic platform for IL-17RA-mediated diseases. (37 refs, 6 figs) (AA)

110770 Integration of biosensors into digital microfluidics: Impact of hydrophilic surface of biosensors on droplet manipulation

Ehsan Samiei, George S. Luka, H o m a y o u n Najjaran, Mina H o o r f a r [Biosensors &

Bioelectronics, v 81, Jul 2016, Starting Page 480, Pages 7] Several studies have been performed on the integration of biosensors into digital microfluidics (DMF). Despite the general success in their detection capabilities, there are still two challenges associated with the integration of biosensors into DMF: (1) complete removal of the droplet containing the analytes from the sensing surface; and (2) biochemical regeneration of the biosensor involving detaching the target analyte from the receptor after each round of sensing. The latter is case dependent and the solution can vary from one application to another. Our research aims at addressing the former, the solution to which is applicable to all biosensors integrated to DMF. This paper presents a thorough characterization of the hydrophilic surface of the biosensor in terms of wettability and geometry, taking into account the overall configuration of the DMF platform. Consequently, we identify the optimal geometry of the sensing surface and the DMF platform providing successful removal of the target droplet from the sensing surface after detection. Based on the results, the gap height is suggested to be chosen at the upper limit of the applicable range. Also, the biosensor, patterned on the device top plate, is recommended to be designed with a high aspect ratio and aligned with the center of the actuating electrode. As a proof of concept, the optimum configuration is implemented on a DMF platform with an interdigitated capacitive biosensor to detect different concentrations of Cryptosporidium, for

which it is shown that the sample droplet is removed successfully from the superhydrophilic surface of the biosensor. (20 refs, 5 figs, 1 table) (AA)

110771 Porous Co3o4 hollow nanododecahedra for nonenzymatic glucose biosensor and biofuel cell

Erhuan Zhang, Yu Xie, Suqin Ci, Jingchun Jia, Zhenhai Wen [Biosensors & Bioelectronics, v 81, Jul 2016, Starting Page 46, Pages 8]

Cobalt oxide hollow nanododecahedra (Co3O4-HND) is synthesized by a facile thermal transformation of cobalt-based metal-organic framework (Co-MOF, ZIF-67) template. The morphology and properties of the Co3O4-HND are characterized by a set of techniques, including transmission electron microscope (TEM), powder X-ray diffraction (XRD), scanning electron microscope (SEM) and Brunner–Emmet–Teller (BET). When tested as a non-enzymatic electrocatalyst for glucose oxidation reaction, the Co3O4-HND exhibits a high activity and shows an outstanding performance for determining glucose with a wide window of 2.0 μM to 6.06 mM, a high sensitivity of 708.4 μA mM−1 cm−2, a low detection limit of 0.58 μM (S/N=3), and fast response time(<2 s). Based on the nonenzymatic oxidation of glucose, Co3O4-HND could be served as an attractive non-enzyme and noble-metal-free electrocatalyst in glucose fuel cell (GFC) due to its excellent electrochemical properties, low cost and facile preparation. (37 refs, 5 figs) (AA)

110772 Novel bi-enzyme electrochemical biosensor for selective and sensitive determination of methyl salicylate

Yi Fang, Yogeswaran U m a s a n k a r , Ramaraja P. R a m a s a m y [Biosensors & Bioelectronics, v 81, Jul 2016, Starting Page 39, Pages 7]

An amperometric sensor based on a bi-enzyme modified electrode was fabricated to detect methyl

Abstracts


salicylate, a volatile organic compound released by pathogen-infected plants via systemic response. The detection is based on cascadic conversion reactions that result in an amperometric electrochemical signal. The bi-enzyme electrode is made of alcohol oxidase and horseradish peroxidase enzymes immobilized on to a carbon nanotube matrix through a molecular tethering method. Methyl salicylate undergoes hydrolysis to form methanol, which is consumed by alcohol oxidase to form formaldehyde while simultaneously reducing oxygen to hydrogen peroxide. The hydrogen peroxide will be further reduced to water by horseradish peroxidase, which results in an amperometric signal via direct electron transfer. The bi-enzyme biosensor was evaluated by cyclic voltammetry and constant potential amperometry using hydrolyzed methyl salicylate as the analyte. The sensitivity of the bi-enzyme biosensor as determined by cyclic voltammetry and constant potential amperometry were 112.37 and 282.82 μA cm−2 mM−1 respectively, and the corresponding limits of detection were 22.95 and 0.98 μM respectively. Constant potential amperometry was also used to evaluate durability, repeatability and interference from other compounds. Wintergreen oil was used for real sample study to establish the application of the bi-enzyme sensor for selective determination of plant pathogen infections. (36 refs, 5 figs, 1 table) (AA)

110773 Sensitive and simple macrophage-based electrochemical biosensor for evaluating lipopolysaccharide cytotoxicity of pathogenic bacteria

X i u m e i Wang, Pei Zhu, Fuwei Pi, Hui Jiang, J i n g d o n g Shao, Yinzhi

Zhang, Xiulan Sun [Biosensors & Bioelectronics, v 81, Jul 2016, Starting Page 349, Pages 9] In this study, a sensitive and simple electrochemical murine macrophage (Ana-1) cell sensor has been developed for early detection of lipopolysaccharides (LPS) to evaluate the toxicity of pathogenic bacteria. Magnetic glassy carbon electrode (MGCE), which possesses excellent reproducibility and regeneration qualities, was modified with a nanocomposite to improve electrochemical signals and enhance the sensitivity. The synthesized magnetic nanoparticles (MNPs)

were internalized into murine macrophages, which completed the immobilization of macrophages onto the modified electrode for evaluating the cytotoxicity of LPS by electrochemical impedance spectroscopy (EIS). The MNPs facilitated reusability of the proposed sensor by allowing removal of the magnetic core from the electrode. Our results indicated that LPS caused a marked decrease in electrochemical impedance in a dose-dependent manner in range of 1–5 μg/mL. By SEM, we found that microvilli on the plasma membrane became scarce and the membrane became smooth on cells incubated with LPS, which lessens the absorption of cells to reduce the impedance. And biological assay indicated that EIS patterns were correlated with the calcium concentration in cells, and suggested that [Ca2+]i production increased in cells incubated with LPS and its mobilization altered electrochemical signals. Compared with conventional methods, this electrochemical test is inexpensive, highly sensitive, and has a quick response, and thus provides a new avenue for evaluating the cytotoxicity of pathogens. (28 refs, 6 figs) (AA)

110774 Real-time monitoring of macromolecular biosensing probe self-assembly and on-chip ELISA using impedimetric microsensors

Faheng Zang, K o n s t a n t i n o s Gerasopoulos, Xiao Zhu Fan, Adam D. Brown, James N. Culver, Reza Ghodssi

[Biosensors & Bioelectronics, v 81, Jul 2016, Starting Page 401, Pages 7] This paper presents a comprehensive study of the self-assembly dynamics and the biosensing efficacy of Tobacco mosaic virus-like particle (TMV VLP) sensing probes using an impedimetric microsensor platform. TMV VLPs are high surface area macromolecules with nanorod structures constructed from helical arrangements of thousands of identical coat proteins. Genetically modified TMV VLPs express both surface attachment-promoting cysteine residues and FLAG-tag antibody binding peptides on their coat protein outer surfaces, making them selective biosensing probes with self-assembly capability on sensors. The VLP self-assembly dynamics were studied by the continuous monitoring of impedance changes at 100 Hz using interdigitated impedimetric microsensors. Electrical impedance

Abstracts


spectroscopy revealed VLP saturation on impedance sensor surface with the coverage of 68% in self-assembly process. The VLP-functionalized impedance sensors responded to 12 ng/ml to 1.2 μg/ml of target anti-FLAG IgG antibodies in the subsequent enzyme-linked immunosorbent assays (ELISA), and yielded 18–35% total impedance increases, respectively. The detection limit of the target antibody is 9.1 ng/ml using the VLP-based impedimetric microsensor. These results highlight the significant potential of genetically modified VLPs as selective nanostructured probes for autonomous sensor functionalization and enhanced biosensing. (26 refs, 6 figs) (AA)

110775 The IsoStretcher: An isotropic cell stretch device to study mechanical biosensor pathways in living cells

S Schürmann, S Wagner, S Herlitze, C Fischer, S Gumbrecht, A Wirth-Hücking, G Prölß, LA Lautscham, B Fabry, WH Goldmann and others [Biosensors & Bioelectronics, v 81, Jul 2016, Starting Page 363, Pages 10]

Mechanosensation in many organs (e.g. lungs, heart, gut) is mediated by biosensors (like mechanosensitive ion channels), which convert mechanical stimuli into electrical and/or biochemical signals. To study those pathways, technical devices are needed that apply strain profiles to cells, and ideally allow simultaneous live-cell microscopy analysis. Strain profiles in organs can be complex and multiaxial, e.g. in hollow organs. Most devices in mechanobiology apply longitudinal uniaxial stretch to adhered cells using elastomeric membranes to study mechanical biosensors. Recent approaches in biomedical engineering have employed intelligent systems to apply biaxial or multiaxial stretch to cells. Here, we present an isotropic cell stretch system (IsoStretcher) that overcomes some previous limitations. Our system uses a rotational swivel mechanism that translates into a radial displacement of hooks attached to small circular silicone membranes. Isotropicity and focus stability are demonstrated with fluorescent beads, and transmission efficiency of elastomer membrane stretch to cellular area change in HeLa/HEK cells.

Applying our system to lamin-A overexpressing fibrosarcoma cells, we found a markedly reduced stretch of cell area, indicative of a stiffer cytoskeleton. We also investigated stretch-activated Ca2+ entry into atrial HL-1 myocytes. 10% isotropic stretch induced robust oscillating increases in intracellular Fluo-4 Ca2+ fluorescence. Store-operated Ca2+ entry was not detected in these cells. The Isostretcher provides a useful versatile tool for mechanobiology. (46 refs, 5 figs) (AA)

110776 Label-free electrochemical lead (II) aptasensor using thionine as the signaling molecule and graphene as signal-enhancing platformFeng Gao, Cai Gao, Suyu He, Qingxiang Wang, Aiqun Wu [Biosensors & Bioelectronics, v 81, Jul 2016, Starting Page 15, Pages 8] A label-free and highly sensitive electrochemical aptasensor for Pb2+ was constructed using thionine (TH) as the signaling molecule and graphene (GR) as the signal-enhancing platform. The electrochemical sensing interface was fabricated by stepwise assembly of GR and TH on the lead (II) specific aptamer (LSA) modified electrode. Upon interaction with Pb2+, the aptamer probe on the sensor underwent conformational switch from a single-stranded DNA form to the G-quadruplex structure, causing the GR with assembled TH released from the electrode surface into solution. As a result, the electrochemical signal of TH on the aptasensor was substantially reduced. Under the optimal experimental conditions, the attenuation of peak currents presented a good linear relationship with the logarithm of Pb2+ concentrations over the range from 1.6×10−13 to 1.6×10−10 M. The detection limit was estimated to be 3.2×10−14 M. The aptasensor also exhibited good regenerability, excellent selectivity, and acceptable reproducibility, indicating promising application in environment monitoring of lead. (45 refs, 5 figs, 1 table) (AA)

110777 Recent advances in salivary cancer diagnostics enabled by biosensors and bioelectronicsSaswat Mishra, Darius Saadat, Ohjin Kwon, Yongkuk Lee, Woon-Seop Choi, Jong-Hoon Kim, Woon-Hong Yeo [Biosensors & Bioelectronics, v 81, Jul 2016, Starting Page 181, Pages 17] There is a high demand for a non-invasive, rapid, and highly accurate tool for disease diagnostics. Recently, saliva based diagnostics for the detection of

Abstracts


s p e c i f i c biomarkers has d r a w n s i g n i f i c a n t attention since the sample extraction is simple, cost-effective, and p r e c i s e .

Compared to blood, saliva contains a similar variety of DNA, RNA, proteins, metabolites, and microbiota that can be compiled into a multiplex of cancer detection markers. The salivary diagnostic method holds great potential for early-stage cancer diagnostics without any complicated and expensive procedures. Here, we review various cancer biomarkers in saliva and compare the biomarkers efficacy with traditional diagnostics and state-of-the-art bioelectronics. We summarize biomarkers in four major groups: genomics, transcriptomics, proteomics, and metabolomics/microbiota. Representative bioelectronic systems for each group are summarized based on various stages of a cancer. Systematic study of oxidative stress establishes the relationship between macromolecules and cancer biomarkers in saliva. We also introduce the most recent examples of salivary diagnostic electronics based on nanotechnologies that can offer rapid, yet highly accurate detection of biomarkers. A concluding section highlights areas of opportunity in the further development and applications of these technologies. (175 refs, 6 figs, 6 tables) (AA)

110778 Increasing the spectral shifts in LSPR biosensing using DNA-functionalized gold nanorods in a competitive assay format for the detection of interferon-γDing-Zheng Lin, Po-Chun Chuang, Pei-Chen Liao, Jung-Po Chen, Yih-Fan Chen [Biosensors & Bioelectronics, v 81, Jul 2016, Starting Page 221, Pages 8] We demonstrate an approach that utilizes DNA-functionalized gold nanorods (AuNRs) in an indirect competitive assay format to increase the spectra shift in localized surface plasmon resonance (LSPR) biosensing. We use interferon gamma (IFN-γ) as a model analyte to demonstrate the feasibility of our detection method. The LSPR chips with periodic gold nanodot arrays are fabricated using a thermal lithography process and are functionalized with IFN-γ aptamers for detection. The DNA-

functionalized AuNRs and IFN-γ compete with each other to bind to the aptamers during detection, and the spectra shifts are mainly caused by the AuNRs rather than IFN-γ. When using our approach, the target molecules do not need to be captured by two capture ligands simultaneously during detection and thus do not require multiple binding sites. Both experiments and finite-difference time-domain (FDTD) simulations show that making the AuNRs as close to the chip surface as possible is very critical for increasing LSPR shifts, and the simulated results also show that the orientation of the AuNR affects the plasmon coupling between the gold nanodots on the chip surface and the nearby AuNRs. Although only the detection of IFN-γ is demonstrated in this study, we expect that the LSPR biosensing method can be applied to label-free detection of a variety of molecules as long as suitable aptamers are available. (38 refs, 6 figs) (AA)

110779 Point-of-care and visual detection of P. aeruginosa and its toxin genes by multiple LAMP and lateral flow nucleic acid biosensor

Yuting Chen, Nan Cheng, Yuancong Xu, Kunlun Huang, Yunbo Luo, Wentao Xu [Biosensors & Bioelectronics, v 81, Jul 2016, Starting Page 317, Pages 7] This study describes a simple and sensitive approach for visual and point-of-care detection of

P. aeruginosa and its toxin genes based on multiple loop-mediated isothermal amplification (mLAMP) and lateral flow nucleic acid biosensor (LFNAB). Differentiation of the internal standard gene ecfX and toxin genes (ExoS and ExoU) in P. aeruginosa was determined using FITC-, hex-and digoxin-modified primers in the mLAMP process. In the presence of biotin-and FITC- (hex-, digoxin-) modified primers and Bst DNA polymerase large fragments, the mLAMP produced numerous biotin- and FITC- (hex-, digoxin-) attached duplex DNA products. The products were detected by LFNAB through dual immunoreactions (anti-biotin antibodies on the gold nanoparticle (Au-NP) and biotin on the duplex, anti-FITC (hex, digoxin)

Abstracts


antibodies on the LFNAB test line and FITC (hex, digoxin) on the duplex). The accumulation of Au-NPs produced a characteristic red band, enabling visual detection of P. aeruginosa and its toxin genes without instrumentation. After systematic optimization of LFNAB preparation and detecting conditions, the current approach was capable of detecting concentrations as low as 20 CFU/mL P. aeruginosa or its toxin genes within 50 min without complicated instrument, which is more sensitive than PCR. Therefore, this approach provides a simple, pollution free, sensitive, and low-cost point-of-care test for the detection of P. aeruginosa and its toxin genes. (23 refs, 4 figs, 2 tables) (AA)

110780 highly sensitive aptasensor for oTA detection based on hybridization chain reaction and fluorescent perylene probe

Bin Wang, Yuanya Wu, Yanfen Chen, Bo Weng, Liqun Xu, Changming Li, [Biosensors & B ioe lect ron ics , v 81, Jul 2016, Starting Page 125, Pages 6] An

optical aptasensor was developed for ultrasensitive detection of ochratoxin A (OTA) based on hybridization chain reaction (HCR) amplification strategy and fluorescent perylene probe (PAPDI)/DNA composites. Dendritic DNA concatamers were synthesized by HCR strategy and modified on magnetic nanoparticles through aptamer as medium. A large amount of PAPDI probe aggregated under the induction of DNA concatamers and caused fluorescence quenching. In the presence of OTA, the PAPDI/DNA composites were released from magnetic nanoparticles due to the strong affinity between aptamer and OTA. In ethanol, PAPDI monomers disaggregated and produced strong fluorescence. The present method displays excellent sensitivity and selectivity towards OTA. (25 refs, 5 figs, 1 table) (AA)

110781 FeMoo4 based, enzyme-free electrochemical biosensor for ultrasensitive detection of norepinephrineKunda J. Samdani, Jitendra S. Samdani, Nam Hoon Kim, Joong Hee Lee [Biosensors & Bioelectronics, v 81, Jul 2016, Starting Page 445, Pages 9] Herein, FeMoO4 (FM) nanorods were synthesized by a template-free, facile, hydrothermal method in an aqueous medium. The surface morphology

of FeMoO4 was identified with field emission s c a n n i n g e l e c t r o n m i c r o s c o p y (FESEM) and t ra n s m i s s i o n e l e c t r o n m i c r o s c o p y (TEM). X-ray

diffraction (XRD) was performed to identify the crystallographic nature of the as-synthesized FeMoO4. The as-synthesized material was used as an active electrode material for the oxidation of a neurotransmitter (i.e. norepinephrine (NE)) by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. FeMoO4 possesses polycrystallanity and bimetallic character, which helps to enhance the performance of the FM/GCE as compared to the GCE. The enhanced performance was also due to the formation of Fe (II)-dioxygen complexes, which catalyze the oxidation of NE. Meticulous observations taken from CV studies proved the diffusion-controlled nature of the reaction with a diffusion coefficient of 1.10×10−4 cm2/s and a standard heterogeneous rate constant of 4.078×10−3 cm/s. The amperometric response of NE on the FM/GCE showed a linear increase in the current between 5.0×10−8 M and 2.0×10−4 M with a detection limit of 3.7×10−9 M. In the amperometric study, the time required to reach the 98% steady state response, after successive additions of 50 nM NE, was less than 3 s. The FM/GCE showed good sensitivity, and stability for the determination of NE. (45 refs, 5 figs, 1 table) (AA)

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Vol. 15 No. 7 July 2016 Editorial Board Contents Technical Papers · 2016-08-17 · Contents...

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