Vitae BELINDA PASTRANA-RIOS - UPRMacademic.uprm.edu/~pastranaslab/docs/bpcv_2011d.pdf · Vitae...

Vitae Updated Nov 2011

BELINDA PASTRANA-RIOS

University of Puerto Rico Department of Chemistry

Mayagüez Campus P.O. Box 9019 tel: (787) 832-4040 x2302 Mayagüez, PR 00681-9019 fax: (787) 265-3849

Chemist Lic.# 4934 [email protected] http://mbrs.uprm.edu

[email protected] http://uprm.edu/pastranalab

Birthplace Bronx, NY Married November 21, 1963

A. EDUCATION 1987 B.S. Chemistry, University of Puerto Rico, Humacao, PR 1992 M.S. Biophysical Chemistry, Rutgers University, NJ 1995 Ph.D. Biophysical Chemistry, Rutgers University, NJ 1995-96 Post-Doc Pharmacology, Biochemistry & Molecular Biology, Mayo Clinic & Foundation, Rochester, MN

B. POSITIONS AND EMPLOYMENT 1984-1987 Research Assistant, Chemistry Dept. University of Puerto Rico, Humacao, PR 1987-1988 Internship as Chemist, Syntex, Humacao, PR 1988-1995 Research Assistant, Chemistry Department, Rutgers University, Newark, NJ 1995-1996 Post-doctorate, Biochemistry and Molecular Biology, and Pharmacology, Mayo Clinic

& Foundation, Rochester, MN 1996-1999 Assistant Professor, Chemistry Department, University of Puerto Rico at Mayagüez 1999-2003 Associate Professor, Chemistry Department, University of Puerto Rico at Mayagüez 1999-present Director, Visualization Center, Chemistry Department, UPR at Mayagüez 2001-present Adjunct Professor, Biology, University of Puerto Rico, Mayagüez, PR 2001-present Adjunct Professor, Biotechnology, University of Puerto Rico, Mayagüez, PR 2002-present Adjunct Professor, Chemical Engineering, University of Puerto Rico, Mayagüez, PR 2003-present Professor, Chemistry Department, University of Puerto Rico, Mayagüez, PR 2007-2008 President of the Biophysics Examination Committee

C. OTHER PROFESIONAL EXPERIENCE 2004-present Reviewer of ACS Biochemistry 2006-present Reviewer of Proteins 2008-present Reviewer for Biophysical Journal 2011-present NIH-CSR Biological Chemistry & Macromolecular Biophysics (BCMB-H) RO1

Awards 2005-2007 NIH-CSR Biochemistry and Biophysics Study Section Committee for Kirshtein

Awards 2006-2007 NIH-SCORE CSR Biochemistry Ad-Hoc Study Section Committee

Vitae Belinda Pastrana-Rios, Ph.D.

D. HONORS 1985 -1987 NIH-MBRS, University of Puerto Rico, Humacao, Puerto Rico 1988 -1995 NIH-Minority Biomedical Research Support (MBRS) Fellowship, Rutgers University, Newark, New Jersey 1995 -1996 NIH-NRSA Kirshtein Postdoctoral Fellow, Mayo Clinic & Foundation,

Rochester, Minnesota 1997 Mayo Clinic & Foundation Visiting Scholar Award Rochester, Minnesota 1998 Mayo Clinic & Foundation Visiting Scholar Award Rochester, Minnesota 1999 ASCB NIH-MARC Visiting Professorship Award at Mayo Clinic &

Foundation, Rochester Minnesota 2002 Invited Attendee at the National Academy of Science, Arthur Sackler

Colloquia at Washington, DC 2002 Who’s Who Among America’s Teachers at the University of Puerto Rico,

Mayagüez Campus 2005 -2007 Who’s Who Among America’s Teachers at the University of Puerto Rico,

Mayagüez Campus 2009 Henry Dreyfus Teacher Scholar Award, University of Puerto Rico,

Mayagüez Campus 2011 International Year of Chemistry (IYC) Award to Distinguished Women in

Chemistry and Chemical Engineering in Puerto Rico 2011 ACS UPRM Student Chapter Distinguished Women Chemist

E. ADDITIONAL COURSES June 1992 Recombinant DNA for Chemists (ACS course) Georgetown University, Washington, DC

June 1995 Molecular Biological Techniques Department of Biochemistry and Molecular Biology Mayo Graduate School of Medicine Mayo Clinic and Foundation, Rochester, Minnesota

August 1996 Teaching and Learning Techniques Center for Professional Enrichment University of Puerto Rico, Mayagüez, Puerto Rico

April 1998 Advanced Computers as Tools for Teaching/Learning UPR Information Systems & Dept. of Chemistry at RUM University of Puerto Rico, Mayagüez, Puerto Rico

August 2001 CharMm Workshop (Accelrys) Supercomputing Center in San Diego, UCSD, San Diego, California

August 2001 Structure Based Drug Design (Accelrys) Supercomputing Center in San Diego, UCSD, San Diego, California

November 2003 A Field Guide to GenBank and NCBI Molecular Biology Resources National Center for Biotechnology Information, UPR, Mayagüez, PR

May 2009 Grant Writing Workshop University of Kentucky, Lexington, Kentucky

July 2009 Tangential Flow Filtration Bioprocess and Development Center, Mayagüez, Puerto Rico


F. RESEARCH EXPERIENCE Summer 1999 American Society for Cell Biologist (ASCB) Visiting Faculty At Dr. J.L. Salisbury Laboratory Dept. of Biochemistry and Molecular Biology Mayo Clinic & Foundation, Rochester, Minnesota Funded by NIH-MARC Summer 1997 & 98 Mayo Clinic Faculty Scholar at Dr. J. L. Salisbury laboratory Dept. of Biochemistry & Cellular and Molecular Biology and the Laboratory of Dr. F.G. Prendergast Dept. of Pharmacology Mayo Clinic & Foundation, Rochester, Minnesota 1995 -1996 Post-Doctoral Research Fellow with Professor J. L. Salisbury Dept. of Biochemistry & Cellular and Molecular Biology and the Laboratory of Dr. F.G. Prendergast Dept. of Pharmacology Mayo Clinic & Foundation, Rochester, Minnesota 1985 -1987 Research Assistant, with Professor I. Nieves, Chemistry Department University of Puerto Rico, Humacao, Puerto Rico 1984 - 1985 Research Assistant, with Professor R. Marrero Chemistry Department University of Puerto Rico, Humacao, Puerto Rico

G. RESEARCH PROPOSALS RESEARCH PROPOSALS GRANTED 1997-1998 SEED MONEY UPR-Mayagüez “The Biophysical Characterization of

Phosphorylated and Unphosphorylated Centrin’s” $ 4,998 1998-1999 Excellence SEED MONEY UPR-Mayaguez “The Biophysical

Characterization of Phosphorylated Centrin” $ 14,499 1998-1999 PR-EPSCoR “Recombinant DNA Technology and the Biophysical

Characterization of Proteins” PI: Dr. Belinda Pastrana, $ 85,000 1998-2001 NSF-EPSCoR “Recombinant DNA Technology and the Biophysical Characterization of Proteins” PI: Dr. Belinda Pastrana $ 29,000 1999-2001 NSF-MRI “Enhancement of the Nuclear Magnetic Resonance Facility” PI:

Dr. E. Meléndez $ 425,000 1999-2003 NIH-SCORE “Conformational Changes in Phosphorylated Proteins” PI: Dr.

Alan Lewis $ 450,000 2000-2004 Howard Hughes “Providing Resources of Major Impact in Science

Education” (PROMISE). PI: Dr. Nanette Diffoot, $ 1,100,000 2001-2002 Biotechnology Program “Bacterial Expression of Centrin T168A/S169A

Recombinant using E. coli BL21 DE3 Codon Plus” PI: Dr. Belinda Pastrana, $ 14,310

2001-2002 GlaxoSmithKline “Interdisciplinary Studies of Biomedical Relevance” PI: Dr. Belinda Pastrana, $ 22,000 1999-2003 PR-EPSCoR “The Visualization Laboratory of the University of Puerto Rico

Supercomputing Facility” PI: Dr. Guy Cormier, $ 800,000 2001-2006 NIH-COBRE “Center for Protein Structure function and Dynamics”

PI: Juan Lopez Garriga, $ 9,600,000 2001 Institutional/PRIDCO “Comprehensive Studies at a Molecular level of

Membrane Proteins and Peptides of Biopharmaceutical Relevance” PI: Dr. Belinda Pastrana, $ 124,000


RESEARCH PROPOSALS GRANTED (Cont.) 2003-2007 NIH-SCORE “Squeeze-out and Re-uptake of Pulmonary Surfactant”

PI: Dr. Govind Nadathur, $ 1,007,516 2003-2007 NIH-SCORE “Conformational Changes in Phosphorylated Proteins”

PI: Dr. Govind Nadathur, $ 1,036,974 2007-2011 NIH-SCORE “Calcium Binding Human Centrosomal Proteins and their

Complexes” PI: Dr. Govind Nadthur, $ 832,000 2009-2014 Henry Dreyfus Teacher Scholar Award “An Interdisciplinary Approach

towards the Study of Proteins and Protein-Protein Interactions: From Bioinformatics to Molecular Biophysics’’ Nominee: Belinda Pastrana $ 60,000

2010-2011 BioSEI “Protein-protein Interactions and the Design of Novel Peptides to aid in Structure Activity Relationship” PI: Belinda Pastrana $ 80,000

2010-2014 NIH-RISE “RISE-2-BEST program at UPRM: Enhancing Biomedical Sciences and Biomedical Engineering in Science and Technology” PI: Juan Lopez-Garriga, $ 5 M Investigator: Belinda Pastrana

RESEARCH PROPOSALS SUBMITTED 2011-2015.1 NSF-MCB BioMolecular Systems Cluster “Effects of Phosphorylation on PPI’s”

PI Pastrana-Rios, B. H. PROFESSIONAL ACTIVITIES

2010-2011 Organizing Committee Member for the International 2DCOS-6 Symposium 2007-2009 President of the Organization Committee for the National 2DCOS 2009 2000-2001 American Chemical Society Regional Chapter, Treasurer 1994-1995 American Association of University Professors (AAUPS) Graduate Student

Representative, Rutgers University, New Jersey 1994-1995 MBRS Advisory board, Rutgers University, New Jersey 1985-1987 MBRS-Student National Organization, Regional Student Representative,

Puerto Rico 1985-1987 MBRS Advisory board, University of Puerto Rico, Humacao, Puerto Rico 1983-1985 ACS Student Chapter & Founding Committee Secretary, University of

Puerto Rico, Humacao, Puerto Rico

I. PROFESSIONAL ORGANIZATIONS American Chemical Society since 1983 Society of Applied Spectroscopy since 1995 Mayo Fellows Association since 1995 American Women in Science since 1998 American Association for the Advancement of Science since 1998 American Society of Cell Biologist since 1999 Sigma Xi (elected member) 2000 Biophysical Society since 2003 Colegio de Químicos since 2003 Protein Science since 2004

J. CONSULTING 2005-present Biotechnology Training Center and Bioprocess Pilot Plant Guanajibo, Mayagüez, PR


K. EDUCATIONAL ACTIVITIES WORKSHOPS Howard Hughes PROMISE Series (2000-2004) Anniversary of the DNA Structure Series (October – December, 2003) 2DCOS 2009 (February 20th 2009) LECTURED COURSES Undergraduate Seminar (CHEM 4125) Biotechnology Practicum (BIND 4905) Undergraduate Research (CHEM 4998) Undergraduate Research (CHEM 4999) Biotechnology Research (BIND 5005) General Biochemistry I (CHEM 5071) General Biochemistry Laboratory I (CHEM 5073) General Biochemistry Laboratory II (CHEM 5074) Special Topics for Graduates (CHEM 6007, 6015, 8995) FT-IR Spectroscopy & Microscopy (CHEM 6395) Advanced Biochemistry (CHEM 6715) Biophysics (CHEM 6016) Graduate Research (CHEM 6998) Graduate Research (CHEM 6999) Graduate Research (CHEM 8998) NEW COURSES DEVELOPED Introduction to Biochemistry (CHEM 4055) Bioinformatics, Proteins, and Protein-protein Interactions (CHEM 8995)

REDESIGNED COURSES General Biochemistry Laboratory II (CHEM 5074) NEW UNDERGRADUATE PROGRAMS Biochemistry Program (currently being developed) GRADUATE LEVEL Ph.D Program Steering Committee EXAM COMMITTEES Ph.D. Cumulative Exam in Biophysics (2005-07) UNDERGRADUATE STUDENTS SUPERVISED IN RESEARCH Luis Pérez Moreno German Lorenzo Brian Ramos Joel Otero Jessica Colón Mariela Reyes Marla Gómez Javier O. Vega Noemí Soto Gisselle Román Vivian Negrón Emilio Iraheta Renier Vélez Victor Torres Muriel Campbell Lynette Sánchez Idalia Rodríguez Mitchelle Vázquez Mariena Silvestry Jessica Ramos Samuel Hernández José Rivera Carlos Marrero Christina Seda Nilsa Méndez Susan Martinez Yalitza Moris Javier Tapia Nancy Guillén Frances Vales Wanda Ocaña Michelle Ríos Vanessa Seiglie Yasmine Valentín Carlos Camacho Rebecca Lebrón Joel Díaz Roberto Pares Andrés Ortiz Anixa Hernández Roxana Cintrón Lalisse Guillén Oscar Quiles Melody López Josué Ríos Naomi Díaz Raquel Nuñez Norma I. Rodríguez Jorge Santiago Pablo Acosta Arginny Soto Arnaldo Zayas Jessica Ríos Cristina López Katia Ortiz Héctor Uriel Vélez Migdalia Feliciano Elisa C. Alfaro Nydia Muñoz Melissa Campbell Abigail Padilla Verónica López Cristina Luiggi Héctor Mártir Amalchi Castillo


UNDERGRADUATE STUDENTS SUPERVISED IN RESEARCH (Cont.) Melisa Ortiz Ambar Delgado Dorian Torres Sol Maldonado Angélica Román Fernando Negrón Kendrick Hernández Pedro Ondina Mara Colón José Robles Mariely Quinones Ana Maria Ortiz Audrey Michelli Brenda Méndez Lizbeth Irizarry Lorraine Hernández Tatiana Maldonado Pedro Ramos Deyson Rodriguez José Montoya Luis Miremont (France) Clémence Cornuau (France) Keishla Rodriguez Tatiana Garcia Iván Vargas Marelis Acevedo Reina Añeses GRADUATE STUDENTS SUPERVISED IN RESSEARCH Mildred Ortiz Wanda Ocaña Jessica de Orbeta Verónica Meza Liliana Sosa Sindia Ramos Myrna Reyes Marie C. Rosado Ruth Almodovar Adalberto Díaz DOCTORAL CANDIDATE SUPERVISED Aslin Rodríguez

POST-DOCTORATES SUPERVISED IN RESEARCH

Ines Placencia, project Title: “Squeeze-out and Re-uptake of Pulmonary Surfactant” (2004) -Current Position- Assistant Professor, Complutense University of Madrid, Spain

Ibon Iloro, project Title: “FT-IR spectroscopy and 2DCOS analysis of sEGFR and its ligands” (2004-2006) -Current Position- Research Scientist, BioGUNE, Bizkaia, Spain RESEARCH ASSISTANTS SUPERVISED Zuleika Sanoguet Daniel Narváez Ana María Gómez

L. RESEARCH ACTIVITIES PUBLICATIONS 1. Benabe, J. E., Echegoyen, L. A., Pastrana, B., Martinez, M., "Mechanism of Inhibition of Glycolisis

by Vanadate"1987 Journal of Biological Chemistry, 282 (20), 9555 - 9560. PMID: 3036865.

2. Pastrana, B., Mautone, A. J., Mendelsohn, R., "Fourier Transform Infrared Studies of Secondary Structure and Orientation of Pulmonary Surfactant SP-C and Its Effect on the Dynamic Surface Properties of Phospholipids"1991 Biochemistry, 30, 10058 -10064. PMID: 1911771.

3. Pastrana, B. "FT-IR Studies of Pulmonary Surfactant SP-C and Its Effect on the Dynamic Surface Properties of Phospholipids." 1992 MS Thesis

4. Pastrana-Rios, B., Flach, C., Braunner, J., Mautone, A. J., Mendelsohn, R., "A Direct Test of the "Squeeze-Out" Hypothesis of Lung Surfactant Function. External Reflection FT-IR at the Air/Water Interface" 1994 Biochemistry, 33, 5121 - 5127. PMID: 8172887.

5. Pastrana-Rios, B. "Lipid/Lipid and Lipid/Protein Interactions in Pulmonary Surfactant Using FT-IR Spectroscopy." 1995 Ph,D. Thesis

6. Pastrana-Rios, B., Taneva, S., Keough, K., Mendelsohn, R., “Secondary Structure and “Squeeze-out” of the Pulmonary Surfactant Proteins SP-B and SP-C in Phospholipid Monolayers at the Air/Water interface. An In Situ External Reflectance-Absorbance FT-IR study” 1995 Biophysical Journal, 69 (6), 2531 - 2540. PMID: 8599660.


INDEPENDENT PUBLICATIONS 7. Graff, D. K., Pastrana-Rios, B., Venyaminov, S., Prendergast, F.G., "Fourier Transform Infrared

Spectroscopy and Two-Dimensional Infrared Analysis of Model Helical Peptides" 1997 Journal of the American Chemical Society, 119, 11282-11294.

8. Pastrana-Rios, B. “Using InsightII® to Provide an Interdisciplinary Approach for Teaching Structural Biology” Accelrys 2002.

9. Pastrana-Rios, B. “Biochemistry Laboratory II”, URL site: http://www.uprm.edu/wquim/q5074,

2001 (copyright)

10. Pastrana-Rios, B. "Understanding the Mechanism of Peptide Unfolding" 2001 Biochemistry, 40, 9074-81. PMID: 11478873.

11. Pastrana-Rios, B., Wanda Ocaña, Michelle Rios, German Lorenzo Vargas, Ginny Ysa, Greg Poynter, Javier Tapia, and Salisbury, J. L., "Centrin: Its Secondary Structure in the Presence and Absence of Cations" 2002 Biochemistry 41 (22), 6911-6919. PMID: 12033923.

12. Pastrana-Rios, B. and López, G. “Mechanism of unfolding of an Alpha-helical Peptide” 2002 Advances in Gene Technology: The Genome and Beyond – Structural Biology for Medicine, 13, 34. (peer reviewed short comm.)

13. Pastrana-Rios, B., Sanoguet, Z. and Ocaña, W. “Secondary Structure Changes in a Calcium Binding Protein: Centrin” 2002 Advances in Gene Technology: The Genome and Beyond – Structural Biology for Medicine, 13, 34. (peer reviewed short comm.)

14. Ocasio, M., Maury-Evertsz, J.R., Pastrana-Ríos, B. and López G.E. “Conformational changes in a model helical peptide: A parallel tempering Monte Carlo study” 2003 J. Chem. Phys. 119, 9274-9279.

15. Pastrana-Rios, B. “Exploring Structure/Function Relationship of Macromolecules at the

Undergraduate Level” 2004 J. Chem. Educ. 81, 837-840.

16. Ortiz, M., Haitau, H., Chazin, W. and Pastrana-Rios B. “Dynamics of Hydrogen/Deuterium exchange in Chlamydomonas Centrin” 2005 Biochemistry 44(7); 2409-2418. PMID: 15709753.

17. Villalobos, L., López, Y., Pastrana-Ríos, B. and López G.E. “Thermodynamics of the liquid states

of Langmuir monolayers” 2005 J. Chem. Phys 122, 104701-7. PMID: 15836339. 18. Sanoguet-Carrero, Z., Campbell, M., Ramos, S., Seda, C., and Pastrana-Rios, B. “Effects of

phosphorylation in Chlamydomonas centrin Ser 167” 2006 Calcium Binding Proteins 1(2), 108-114.

19. Meyn, S.M.; Seda, C.; Campbell, M.; Weiss, K.L.; Hu, H., Pastrana-Rios, B.; and Chazin, W. J. “The Effect of Ser 167 Phosphorylation on Chlamydomonas reinhardtii Centrin” 2006 Biophys. Biochem. Res. Comm. 342, 342-348. PMID: 16480960.

20. Iloro, I.; and Pastrana-Ríos, B., “Simulation of FT-IR Spectra and 2D-COS Analysis for the H/D Exchange of Two Related Ligands” 2006 J. Molecular Structure: Special issue of 2D-COS, 799, 153-157.


21. Pastrana-Ríos, B., “Simulation of FT-IR Spectra and 2D-COS Analysis for the Thermal Perturbation of Apo-Centrin” 2006 J. Molecular Structure: Special issue of 2D-COS, 799, 163-167.

22. Iloro, I.; Narváez, D; Guillen, N; Camacho, C; Guillen, L; Cora E; and Pastrana-Ríos, B., “Kinetics of Hydrogen/Deuterium Exchange of EGFR ligands” 2008 Biophysical Journal 94, 4041-4055. PMID: 18199660.

23. E. Alfaro, L. D. V. Sosa, Z. Sanoguet, B. Pastrana-Rios, and E. R. Schreiter “Crystallization and Preliminary X-ray Characterization of full-length Chlamydomonas reinhardtii centrin” 2008 Acta Cryst. F 64, 398 - 401. PMID: 18453711.

24. Sosa, L. D.V., Alfaro, E., Santiago, J., Narváez, D., Rosado, M.C., Rodríguez, A., Gómez, A.M., Schreiter, E., and Pastrana-Rios, B. “The Structure, Molecular Dynamics, and Energetics of Centrin-Melittin Complex” 2011 Proteins: Structure, Function, and Bioinformatics 79, 3132-3143. PMID: 21989934.

25. Ocaña, W., and Pastrana-Rios, B. “Calcium titration of Chlamydomonas centrin and its structural changes” 2011 (Submitted to: J. Molec. Vibrations).

26. Sosa, L. D.V., Santiago J., Narváez, D., Santiago, J., Martinez, N. and Pastrana-Rios, B. “Centrin-Melittin Complex a model for protein-protein interactions and amyloidogenesis” (in preparation Proteins).

27. Reyes, M. Meza, V., Orbeta, J. Díaz, A., Almodovar, R., Narváez, D., and Pastrana-Rios, B.

Relative stability of Centrins (in Preparation PNAS) 28. Ramos, S; López, G.E., Pastrana-Rios, B., “Lung surfactant SP-C Analogues and Model Lipid

Interactions” (In preparation).

PUBLISHED STRUCTURES 29. Chlamydomonas reinhardtii centrin-Melittin complex (2011) PDB ID: 3QRX

PUBLISHED ABSTRACTS (PEER REVIEWED) 30. Mildred Ortiz, Belinda Pastrana, Haitao Hu, Walter Chazin, “H/D Exchange FT-IR Spectroscopic

Study of Chlamydomonas Centrin Domains” 2001 Molec. Biol. Cell 12, 27a. 31. Julianne del Toro, and Belinda Pastrana-Rios, “Expression of Chlamydomonas centrin monitored

via FT-IR Spectroscopy”, 2001 Molec. Biol. Cell 12, 27a. 32. Wanda Ocaña Rivera, Belinda Pastrana-Rios, J.L. Salisbury, Walter Chazin, “Ca2+ and Mg2+

Binding Constants in Chlamydomonas Centrin”, 2001 Molec. Biol. Cell 12, 27a. 33. Vélez A. and Pastrana-Rios, B. “Explaining Enzymatic Activity Through Phylogeny in a Cell

Biology Course” 2003 Molec. Biol. Cell 31. Narvaez D., Guillen, N. and Pastrana-Rios, B. ”ErbB1 Family of Ligands Fast

Hydrogen/Deuterium Exchange kinetics” 2003, Molec. Biol. Cell 14, 13A. 34. Ortiz, M., Chazin, W.J., Pastrana-Rios, B.” H/D Exchange of Chlamydomonas Centrin Domains

using FT-IR Spectroscopy” 2003 Meeting Abstracts. Biophysical Journal, Supplement, Abstract 764 Poster.

35. Sanoguet Z. and Pastrana-Rios B. “FT-IR Characterization of Phosphorylated Chlamydomonas Centrin” 2003 Meeting Abstracts. Biophysical Journal, Supplement, Abstract 1631-Poster.


36. Seda, C., Sanoguet-Carrero, Z., Campbell, M., Lebrón, R. and Pastrana-Rios, B. “Understanding the Effect of Phosphorylation in Recombinant Chlamydomonas Centrin” 2004 Protein Science,13 (suppl. 1), 150.

37. Narvaez, D., Camacho, C., Ferguson K. M. and Pastrana-Rios B. “Hydrogen/Deuterium Exchange Studies of Recombinant Soluble Epidermal Growth Factor Receptor (sEGFR)” 2004 Protein Science, 13 (suppl. 1), 216.

38. Román, G., Ortiz, M., Sanoguet-Carrero, Z. and Pastrana-Rios, B. “H/D Exchange of Calmodulin and Chlamydomonas Centrin Domains Using FT-IR Spectroscopy and Two-dimensional Correlation Analysis” 2004 Protein Science, 13 (suppl. 1), 217.

39. Guillen, N., Camacho, C. M., Narvaez, D., Cora, E. and Pastrana-Rios, B. “EGFR family of ligands hydrogen/deuterium exchange” 2004 Protein Science, 13 (suppl. 1), 162.

40. Sanoguet-Carrero, Z., Campbell, M., Lebrón-Rodriguez, R., and Pastrana-Rios, B. “Characterization of Phosphorylated Recombinant Chlamydomonas Centrin” 2004 Meeting Abstracts. Biophysical Journal, Supplement, Abstract

41. Camacho, C., Narvaez, D., Fergusson, K. and Pastrana-Rios, B. “Recombinant sEGFR H/D exchange studies using ATR FT-IR Spectroscopy” 2004 Meeting Abstracts. Biophysical Journal, Supplement, Abstract .

42. Sanoguet-Carrero, Z., Campbell, M., Lebrón-Rodriguez, R., and Pastrana-Rios, B. “Characterization of Phosphorylated Recombinant Chlamydomonas Centrin” 2004 Meeting Abstracts. Biophysical Journal, Supplement, Abstract

32. Ramos, S., López, G. and Pastrana-Rios, B. “Infrared and Calorimetric Studies of Surfactant Protein C Analogues” 2005 Protein Science 14 (suppl. 1), 92.

33. Sosa, L. and Pastrana-Ríos, B. “Chlamydomonas 13C-Centrin and Melittin Interactions” 2005 Protein Science 14 (suppl. 1), 160.

34. Campbell, M., Sanoguet Carrero, Z., and Pastrana-Ríos, B. “Evidence of Self Association And Relative Thermal Stability Of Phosphorylated And Unphosphorylated Centrin” 2005 Protein Science 14 (suppl. 1), 164.

35. Seda, C., Campbell, M., Weiss, K., Chazin, W., and Pastrana-Ríos, B. “Structural Differences Upon Phosphorylation of Chlamydomonas Centrin” 2005 Protein Science 14 (suppl. 1), 164.

36. Iloro, I., Narváez, D., Camacho, C., Cora, E. and Pastrana-Ríos, B. “Dynamics of H/D Exchange In EGF, TGF-Alpha and Heparin-Binding EGF: A 2DCOS Study” 2005 Protein Science 14 (suppl. 1), 249.

37. Pastrana-Ríos, B. and Sanoguet, Z. “Phosphorylation Effects of a Calcium Binding Protein: A 2DCOS Study” 2005 Protein Science 14 (suppl. 1), 249.

38. Narváez, D., Camacho, C., Guillen, N., Cora, E. and Pastrana-Ríos, B. “Dynamics of Epidermal Growth Factor Receptor Ligands During Thermal Denaturion” 2005 Protein Science 14 (suppl. 1), 249.

39. López, G., Ramirez, E., Santana, A., Cruz, A., Pastrana-Ríos, B. and Plascencia, I. “Molecular Dynamics of Surfactant Protein C In A Hydrophillic Media 2005 Protein Science 14 (suppl. 1), 251.

40. Meza, V., Pastrana-Rios, B. “Calorimetric Properties of A Centrosome Associated Protein: Human Centrin 2” 2005 Molec. Biol. Cell 633a.

41. Sanoguet, Z., Campbell, M., Pastrana-Rios, B. “Characterization of Centrin an MTOC’s Protein” 2005 Molec. Biol. Cell, 633a.

42. Narváez, D., Gómez, A., Mártir, H., Méndez, N., and Pastrana-Ríos, B. “Human Centrin’s Stability and Their Protein-protein Interactions” ASCB, December 13-17, 2008,

43. Pastrana-Ríos, B. “A Comparative Analysis of Essential Centrosome Associated Complexes and their Variants” Biophysical Meeting, February 28-March 3, 2009,

44. Pastrana-Ríos, B. “2D IR Correlation Spectroscopy a Proven Method for the Characterization of Proteins and Protein-protein Interactions” ICAVS-6 June 12-17, 2011


US PATENT APPLICATIONS 1. Invention Docket Number US12/102,862 Method Patent: Determination of Protein, Peptide or Peptoid Aggregation, Stability and Viability

URL SITES http://www.uprm.edu/wquim/q5074 http://www.uprm.edu/pastranalab

SELECTED PRESENTATIONS 1. Pastrana, B., and Marrero, R. Reaction of -silylesters and silylketones with bromine and

meta-chloro per benzoic acid (MCPBA) in the production of chiral products. 19th ACS Junior Technical Meeting, April 28, 1984, Rio Piedras, PR

2. Pastrana, B., Nieves, I. and Echegoyen, L. Determination of binding constants with respect to protein (Ca2+ -Receptor) and Vanadyl by EPR. 20th ACS Junior Technical Meeting, March 23, 1985, Humacao, PR.

3. Pastrana, B., Moreno, H., Nieves, I., and Echegoyen, L. Binding constant determination between Vanadyl and Serum Bovine Albumin. 13th NIH-MBRS Symposium, March 31- April 4, 1985, Miami, FL.

4. Pastrana, B., Nieves, I., Lopez, C., and Santiago, G. Interaction of Vanadyl with Calmodulin by EPR. 21st ACS Junior Technical Meeting, April 26, 1986, Rio Piedras, PR.

5. Pastrana, B., Nieves, I., Lopez, C., and Santiago G. Determination of binding constant between Vanadyl and Calmodulin by ESR, 192nd ACS National Meeting, September 7-12, 1986, Anaheim, CA.

6. Pastrana, B., Nieves, I., Lopez, C., and Santiago G. Studies of interaction between Vanadyl and Calmodulin by ESR, 22nd ACS Junior Technical Meeting, March 21, 1987, Mayaguez, PR.

7. Pastrana, B., Reilly, K.E., Mendelsohn, R., and Mautone, A. J. Surfactant protein-lipid interactions by FT-IR, 17th NIH-MBRS Symposium, October 5-7, 1989, Houston, TX

8. Pastrana, B., Mautone, A. J., and Mendelsohn, R. Fourier transform infrared studies of secondary structure and orientation of pulmonary surfactant SP-C and its effect on the dynamic surface properties of phospholipids, Northeast Regional NIGMS MARC/MBRS Meeting, September 26, 1992, Bronx, NY.

9. Pastrana, B., Flach, C., Brauner, J.W., Mautone, A.J., and Mendelsohn, R. Direct test of the "squeeze-out" hypothesis of pulmonary surfactant function in situ at the air/water interface, 38th Biophysical Society Meeting, March 6-10, 1994, New Orleans, LA.

10. Pastrana, B., Mendelsohn, R., Mautone, A.J., Taneva, S., and Keough, K.M.W. FT-IR external reflectance test of the “squeeze-out” hypothesis of pulmonary surfactant function. FASEB-Experimental Biology 95, April 9-12, 1995, Atlanta, GA

11. Graff, D. K., Pastrana, B., Prendergast, F.G. Two-dimensional FT-IR correlation analysis of the thermal denaturation of peptide helices, Beyond Protein Structure, Beckman Institute, University of Illinois, June 6-9, 1996, Urbana, IL.


SELECTED PRESENTATIONS (Cont.) 12. Pastrana-Rios, B. Biophysical study of Chlamydomonas centrin, ACS XXI Senior Technical

Meeting, October 31- November 1st 1997, Lajas, PR.

31. Pastrana-Rios, B., Ysa, G., Vélez, R., FT-IR Spectroscopy and Two-dimensional Correlation Analysis of a Calcium Binding Protein: Chlamydomonas Centrin ACS XXII Senior Technical Meeting, November 13-14, 1998, Aguadilla, PR.

32. Pastrana-Rios, B., Negrón, V., Mutations of Human Centrin 2. 11th AAAS & EPSCoR Meeting, April 30th-May 2, 1999, Ponce, PR.

33. Pastrana-Rios, B., FT-IR Spectroscopy and Two-dimensional Correlation Analysis of centrin. AAAS & Oak Ridge National Labs, August 17th, 1999, Oak Ridge TN.

34. Pastrana-Rios, B., Negrón, V., Pointer, G., & Salisbury, J.L., Mutations of Human Centrin 2 ACS XXIII Senior Technical Meeting, November 5-6, 1999, Humacao, PR.

35. Pastrana-Rios, B. American Society of Cell Biologist Meeting, December 11-16th, 1999, Washington, DC.

36. Pastrana-Rios, B., ACS XXIV Senior Technical Meeting, November 3-4, 2000, Aguadilla, PR

37. Pastrana-Rios, B., “Centrin: Its Secondary Structure” 12th AAAS & EPSCoR Meeting, April 2001, Dorado, PR.

38. Pastrana-Rios, B. and López, G. “Mechanism of unfolding of an Alpha-helical Peptide” The Genome and Beyond – Structural Biology for Medicine Symposia, February 2-6, 2002, Miami, FL.

39. Pastrana-Rios, B., Sanoguet, Z., and Ocaña, W. “Secondary Structure Changes in a Calcium Binding Protein: Centrin” The Genome and Beyond – Structural Biology for Medicine Symposia February 2-6, 2002, Miami, FL.

40. Pastrana-Rios, B. Arthur M. Sackler Colloquium on Self-perpetuating Structural States in Biology, Disease and Genetics at the National Academy of Science, March 22-24, 2002, Washington, DC.

41. Pastrana-Rios, B. “Structural Changes and Self association in Chlamydomonas centrin upon Ca2+ Titration” ACS 26th Senior Technical Meeting, August 6-7, 2002, San Juan, PR.

42. Pastrana-Rios, B. “Teaching Bioinformatics and Structural Genomics in an Undergraduate Biochemistry Laboratory” ACS 26th Senior Technical Meeting, August 6-7, 2002, San Juan, PR.

43. Pastrana-Rios, B. and Ortiz, M. “H/D Exchange of Chlamydomonas Centrin Domains Using FT-IR Spectroscopy” Biophysical Meeting, March 1-3, 2003, San Antonio Texas.

44. Pastrana-Rios, B. “Domain Specific Dynamics in a Calcium Binding Protein Leading Towards Biological Function” Spring Conference in Protein Research, April 16th, 2004. San Juan, PR

45. Pastrana-Rios, B. “A Calcium Binding Protein and its Role in Cell Division and Cancer” Biotechnology Meeting, October 2nd, 2004, Mayagüez, PR

46. Pastrana-Ríos, B. and Sanoguet, Z. “Phosphorylation Effects of a Calcium Binding Protein: A 2DCOS Study” 19th Symposium of the Protein Society, July 30th – August 3rd, 2005, Boston, MA


47. Narváez, D., Gómez, A., Mártir, H., Méndez, N., and Pastrana-Ríos, B. “Human Centrin’s Stability and Their Protein-protein Interactions” ASCB, December 13-17, 2008, San Francisco, CA

48. Pastrana-Ríos, B. “A Comparative Analysis of Essential Centrosome Associated Complexes and their Variants” Biophysical Meeting, February 28-March 3, 2009, Boston, MA

49. Arrondo, JLR*, García Pacios, M., de la Arada, I., Goñi, FM y Pastrana-Ríos, B. “Una Nueva Aproximación al studio de espectros de infrarrojo de Biomoléculas. La remoción de bandas” Agosto 2010, Madrid, España.

50. García Pacios, M., Pajares, MA., Goñi, FM., Pastrana-Ríos, B. y Arrondo, JLR “Estudio Infrarrojo de BHMT por 2DCOS y Remoción de Bandas” Agosto 2010, Madrid, España

51. Pastrana-Rios, B. “The Centrin-melittin Complex is a Suitable Model for Protein-protein Interactions and Amyloidogenesis” Research Frontiers in the Chemical Sciences: A Dreyfus Foundation Teacher-Scholar Symposium, October 29th, 2010, New York Academy of Sciences, NY, NY.

52. Pastrana-Ríos, B. “2D IR Correlation Spectroscopy a Proven Method for the Characterization of Proteins and Protein-protein Interactions” ICAVS-6 June 12-17, 2011, Sonoma, CA.

53. Pastrana-Ríos, B. “Centrin-Sfi1p21 complex key to understanding ciliopathies” 60 years and 60 carbons: A Symposium on Fullerenes and Supramolecular Chemistry 43rd IUPAC, July30 - August 7, 2011, San Juan, PR.

INVITED SEMINARS Title: "A Direct Test of the "Squeeze-Out" Hypothesis of Lung Surfactant Function. External Reflection FT-IR at the Air/Water Interface" at: 1. Mayo Clinic, Rochester, MN, March 8th, 1996 2. UPR-Humacao, Humacao, PR, November 15th, 1995 3. UPR-Mayagüez, Mayagüez, PR, November 20th, 1995 Title: “Protein Conformational Changes” at: 4. UPR-Mayagüez, Mayagüez, PR, February, 1998 Title: “Biophysical Studies of an -helical Peptide” at: 5. UPR-Mayagüez, Mayagüez, PR, February, 1998 Title: “Centrin its Secondary Structure in the Presence and absence of cations” at: 6. Mayo Clinic and Foundation, Rochester, MN, June, 1998 Title: “CD and Sedimentation Analysis of Centrin” at: 7. Mayo Clinic and Foundation, Rochester, MN, August 4, 1999 Title: “Centrin: Its Secondary Structure in the Presence and Absence of Cations” at: 8. Vanderbilt University, Nashville, TN, April, 2001 Title: “Mechanism of Unfolding of a Model Helical Peptide” at: UPR-Medical Sciences, 9. Rio Piedras, PR, August 28, 2001 Title: “Bioinformatics” at: UPR-Mayagüez Campus, Biotechnology Program 10. Mayagüez, PR, September 28, 2001 Title: “Why study Proteins?” at: UPR-Mayagüez Campus, Chemistry Dept. 11. Mayagüez, PR, January 22, 2001 Title: “Proteins and life” at: UPR-Humacao Campus, Biology Dept.

12. Humacao, PR, February 16, 2002


INVITED SEMINARS (cont.) Title: “Bioinformatics and Entrepreneurship” at: UPR-Mayagüez Campus, Business Admin. Dept. 13. Mayagüez, PR, April 25, 2002 Title: “Unfolding of a Helical Peptide” at: UPR-Humacao Campus, Chemistry Dept. 14. Humacao, PR, March 11, 2003 Title: “The Conformational Dynamics of a Calcium Binding Protein:Centrin” Ponce Medical School, 15. Ponce, PR, April 2nd, 2003 Plenary Lecture Title: “Learning through the Eyes of a Protein: GFP” University of Puerto Rico

16. Mayaguez, P.R., October 21, 2003 Title: “Learning through the Eyes of a Protein: GFP” University of Puerto Rico Mayaguez, P.R.,

17. November 6, 2003 Plenary Lecture Title: “Biomedical Research a Viable Career” Sigma Xi at the University of P.R.

18. Mayaguez, PR, April 1, 2004 Title: “An Empirical and Simulated Phospholipid Monolayer Study” at the Third International

Vibrational Spectroscopy of Monolayer Films (VSM-3) workshop, University of 19. Laval in Quebec, Canada, July 24-29, 2005.

Title: “A Lesson in Protein Dynamics” at the Third International Symposium on Two-dimensional 20. Correlation Analysis (2DCOS-3), Delavan, Wisconsin, August 11-14, 2005.

Title: “Visualization, Molecular Modeling and Protein Dynamics in Structure Activity Relationship” 21. CoHemis, Mayaguez PR 2006 Plenary Lecture Title: “Centrosomal Associated Protein Interactions and Dynamics: Key to

understanding Function” EXPO CHEM 2006 22. Mayaguez, PR, November 9-11, 2006.

Lecture Title: “Protein-protein interactions and cell division” Chemistry Department UPR Humacao, 23. Humacao, PR February 22, 2007

Lecture Title: “Biotechnology and Research” Biotechnology Week UPR-Mayaguez Campus 24. Mayaguez, PR September 19th, 2007.

Lecture Title: “The Study of Protein Interactions and Ligand Families by FT-IR and 2DCOS” Department of Biochemistry and Molecular Biology at the University of the 25. Basque Country, Bilbao, Spain April 3, 2008.

Lecture Title: “Centrosome Associated Protein Complexes and their Variants” 28th Latin American 26. Congress in Chemistry (FLAQ) San Juan, PR July 27 – Aug. 1st, 2008

Lecture Title: “Exploring Structure/Function Relationship of Macromolecules at the Undergraduate Level” 28th Latin American Congress in Chemistry and Latin American 27. Federation of Chemical Assoc. San Juan, PR July 27 – Aug. 1st, 2008

Lecture Title: “The Use of Two-dimensional Correlation Spectroscopy to Study Centrosomal Proteins and Protein Interactions” Federation of Analytical Chemistry Symposium 28. (FACCS) 2008 Reno, NV, Sept. 28 – Oct. 2, 2008

Plenary Lecture Title: “The study of Proteins and Protein Interactions by 2DCOS” 29. National 2009 2DCOS Symposium, Rincón, Puerto Rico, Feb. 20-22, 2009

Lecture Title: “2D IR Correlation Spectroscopy for the Study of Protein-Protein Interactions” First Lilly Technical Forum 30. Eli Lilly 2011 Carolina, PR, April 15, 2011

Plenary Lecture Title: “Understanding Protein Relative Stability & PPI’s through 2DCOS” 31. International 2DCOS-6 Symposium, Sonoma, California, June 9-12, 2011

Lecture Title: “Is melittin a model for calcium binding protein target peptides?” 32. Chemistry of Life: 43rd IUPAC, San Juan, PR, July 30 - August 7, 2011

Lecture Title: “Slow Cook Your Road to Success” 33. Women’s Chemist Committee, Dorado, PR, November 4, 2011


COLLABORATIONS Dr. F.G. Prendergast (helical peptides) Mayo Clinic Foundation 1996-present Dr. JL Salisbury (centrin) Mayo Clinic Foundation 1996-present Dr. Jorge Steiner (crystal structure) UPR-Mayagüez 1998-present Dr. Elsa Cora (s110kD-EGF) UPR-Med. Sci. 2000-present Dr. Walter Chazin (centrin domains) Vanderbilt University 2000-present Dr. Cynthia McMurray (centrin) Mayo Clinic 2000-present Dr. Gustavo López (peptide simulations) UPR-Mayagüez 2000-present Dr. Jesus Pérez Gil (surfactant proteins) Universidad Complutense 2002-present Dr. Nita Maihle (s110kD-EGF) Mayo Clinic Foundation 2001-present Dr. Erik Goormaghtigh membrane proteins Free University of Brussels 2003-present Dr. Jose Luis Arrondo membrane proteins Univ. of the Basque Country 2004-present MASTERS THESIS 1. Mildred R. Ortiz Hernández 2002 “Attenuated Total Reflectance Fourier Transform Infrared

Spectroscopic Study of Chlamydomonas Centrin during Hydrogen/Deuterium Exchange” - Current Position – Senior Manager Analytical Services, TEVA, Miami, FL

2. Wanda Ocaña 2002 “Calcium and Magnesium Binding Characteristics of Chlamydomonas Centrin Domains” - Current Position - Bioprocess Supervisor, Eli Lilly Biotechnology, Carolina, PR

3. Jessica De Orbeta 2005 “High Level Expression, Purification and Thermal Characterization of Human Centrin 1 (Hcen1)” - Currently- Pursuing Graduate studies towards Ph.D. degree at South Texas University-MD Anderson

4. Verónica Meza Venencia 2005 “Expression, Purification and Characterization of a Human Calcium Binding Protein: Human Centrin 2 (Hcen2)” - Currently- Pursuing MD Residency at UPR-Medical Sciences, San Juan, PR

5. Liliana Del Valle Sosa 2006 “Structural Changes of 13C –labeled Chlamydomonas centrin and Mellitin upon complex formation in the Presence of Calcium” - Currently- Pursuing Graduate studies towards Ph.D. degree at University of Cordova, Argentina

6. Sindia Ramos-Torres 2006 “Surfactant Protein C Analogues and Model Lipid Interactions in Lung Surfactant” - Current Position - Instrumentation Specialist, Chemistry Department, UPRM

7. Myrna Reyes Blas 2008 “Comparative Analysis of Two Human Isoforms Centrin 1 and Centrin 2”

STUDENT THESIS COMMITTEES (Year Degree Awarded; Affiliation) MASTERS Maria Isabel Meléndez Santana, MS (1996) “Identification of Halophilic Bacteria by Volatile Halogenated Organic Compounds” Department of Chemistry at the University of Puerto Rico Mayagüez Campus Walleska De Jesus Bonilla, MS (1997) “Studies of Cytochrome C Oxidase-N2O Complex: A Possible Role in the Mechanism for N2O Anesthesia” Department of Chemistry at the University of Puerto Rico Mayagüez Campus Zuleika Sanoguet, MS (1997)


“Purification and Characterization of Lucina Pectinata Hemoglobin 1 (Hb1)” Department of Chemistry at the University of Puerto Rico Mayagüez Campus DOCTORAL José Ángel Fernández Higuero, Ph.D. “Structural Studies of Human Lipoproteins of Low Density by FT-IR Spectroscopy” Department of Biochemistry and Molecular Biology at the University of the Basque Country, Bilbao, Spain April 2, 2008 Igor de la Arada Echevarría, Ph.D. “Studies of Amyloid fibril formation by Infrared Spectroscopy” Department of Biochemistry and Molecular Biology at the University of the Basque Country, Bilbao, Spain October 16, 2009 UNDERGRADUATE AWARDS PI’s undergraduate and graduate students involved in summer research projects abroad: To DATE >93% of my undergraduate students have pursued GRADUATE STUDIES or MD degrees.

1997-98 Mayo Summer Scholar Luis Pérez Moreno. 1998 Mayo Summer Scholar awarded to Mariela Reyes, Vivian Negrón, Lynette Sánchez.

1999 Mayo Summer Scholar awarded to Vivian Negrón, Susan Martinez, Wanda Ocaña, & German Lorenzo

2000 Vanderbilt Bridges Summer Research awardees Mildred Ortiz & Julianne del Toro 2001 Mayo Clinic International Summer Research awarded to Emilio Iraheta

2002 Univ. Pennsylvania Summer program awarded to Idalia Rodriguez 2002 Univ. Massachusetts Summer Program awarded to Mariena Ramos

2003 Mayo Summer Scholar awarded to Jessica Colón and Gisselle Román 2003 Vanderbilt Summer Fellowship Muriel Campbell

2003 Rutgers University Summer Fellowship Mitchelle Vázquez 2003-04 NIH-UGSP Gisselle Román

2005 Vanderbilt Summer Fellowship Christina Seda 2006 Vanderbilt Summer Fellowship Jessica Ramos 2008 Vanderbilt Summer Fellowship Amalchi Castillo

2008 Mayo Summer Scholar Oscar Quiles 2008 Sloan Kettering Summer Scholar Nilsa Mendez 2008 Broad Institute Summer Scholar Nicole Martinez

2009 Broad Institute Summer Scholar Amalchi Castillo 2009 École Supérieure de Chimie Physique Electronique de Lyon Summer Fellowship,

France Jorge Santiago 2009 MD Anderson Summer Fellowship Melissa Campbell

2010 Vanderbilt Summer Fellowship Amalchi Castillo 2011 Mayo Summer Scholar awarded to Ana María Ortiz

2010 NIH-RISE Lorraine Hernández


STUDENT PRESENTATIONS 1. Over-expression of the Protein Human Centrin 2. Gómez, Marla and Pastrana-Rios, Belinda,

33rd ACS XXI Junior Technical Meeting, March 7th 1998, Bayamón, PR. 2. Mutations of Human Centrin II. Vivian Negrón, B. Pastrana-Rios, and J. L. Salisbury,

National Minority Research Symposium, November 21-24, 1998, NY, NY. I. ACS-JTM at UPR-RUM on March 13th, 1999

3. Mutations of Human Centrin 2. Vivian Negron†, B. Pastrana-Rios†, J.L. Salisbury§, and G. Poynter§. †University of Puerto Rico-Mayagüez Campus, Dept. of Chemistry and §Mayo Clinic and Foundation, Dept. of Biochemistry and Molecular Biology.

4. Bacterial Expression of Variant T169A/S170A Human Centrin 2 Protein José Rivera-Feliciano† Vivian Negrón†, G. Poynter§, J.L. Salisbury, Ph.D.§, and B. Pastrana, Ph.D.†, †University of Puerto Rico-Mayagüez Campus, Dept. of Chemistry and §Mayo Clinic and Foundation, Dept. of Biochemistry and Molecular Biology.

5. Bacterial Expression of Variant L171G/F172G Human Centrin 2 Protein Samuel Hernández† Vivian Negrón†, G. Poynter§, J.L. Salisbury, Ph.D.§, and B. Pastrana, Ph.D.†, †University of Puerto Rico-Mayagüez Campus, Dept. of Chemistry and §Mayo Clinic and Foundation, Dept. of Biochemistry and Molecular Biology.

6. Bacterial Expression of Wild Type Human Centrin 2. Susan Martínez, Yalitza Moris, Belinda Pastrana, Ph.D. University of Puerto Rico, Mayagüez Campus, Dept. of Chemistry,

7. Bacterial Expression of Chlamydomonas Centrin and its Isolation, German Lorenzo Vargas, Wanda Ocaña, Lynnette Sánchez, Belinda Pastrana, Ph.D., University of Puerto Rico-Mayagüez Campus, Dept. of Chemistry.

8. Purification and Biochemical Characterization of Chlamydomonas Centrin from Transformed E. coli BL21 DE3, Wanda Ocaña, German Lorenzo Vargas, Lynnette Sánchez, Belinda Pastrana, Ph.D., University of Puerto Rico-Mayagüez Campus, Dept. of Chemistry.

9. Concentration Determination of Chlamydomonas Centrin Michelle Rios, Belinda Pastrana, Ph.D., University of Puerto Rico-Mayagüez Campus, Dept. of Chemistry.

10. Chlamydomonas Centrin Crystals for X-ray Diffraction Brian Ramos†, Jorge Rios Steiner, Ph.D.§, and Belinda Pastrana, Ph.D.†. †University of Puerto Rico-Mayagüez Campus, Dept. of Chemistry, §Michigan State University, Chemistry Dept.

11. FT-IR Spectroscopy and Two-dimensional Correlation Analysis of Chlamydomonas Centrin. Renier Velez-Cruz, & Belinda Pastrana, Ph.D. Chemistry Department, Box 9019, University of Puerto Rico, Mayagüez Campus, Mayagüez, PR 0681-9019

12. Molecular Modeling Studies of Peptide Analog and a Dodecanucleotide. Javier O. Vega, Belinda Pastrana, Ph.D., Marisol Vera, Ph.D. & Cynthia Robledo, Ph.D. Chemistry Department, Box 9019, University of Puerto Rico, Mayagüez Campus, Mayagüez, PR 0681-9019

II. Mayo Clinic Student Presentations on August 4, 1999 at Rochester, MN

13. Chlamydomonas Centrin: Biophysical Characterization. Susan Martinez†, Belinda Pastrana, Ph.D. † & J.L. Salisbury§. †University of Puerto Rico-Mayagüez Campus, Dept. of Chemistry and §Mayo Clinic and Foundation, Dept. of Bochemistry and Molecular Biology.

14. Use of Yeast two-Hybrid System to identify Protein-Protein Interactions of Axonemal Heavy Chain Dynein Arm with mouse centrin 1. German Lorenzo Vargas†, Wanda Ocaña†, Belinda Pastrana, Ph.D. † & J.L. Salisbury, Ph.D. §. †University of Puerto Rico-Mayagüez Campus, Dept. of Chemistry and §Mayo Clinic and Foundation, Dept. of Bochemistry and Molecular Biology.

III. Academic year 1999-2000

15. Purification and Biochemical Characterization of Chlamydomonas Centrin from Transformed E. coli BL21 DE3, German Lorenzo Vargas, Wanda Ocaña, Lynnette Sánchez, Belinda Pastrana, Ph.D., 1999 National Minority Research Symposium, November 10-12, 1999, Phoenix, Arizona.


IV. ACS-JTM at UPR-RUM on March 13th, 2000 16. Expression and Purification of Variant Human Centrin 2 (S170A). Joel Otero, and Belinda

Pastrana-Rios, Ph.D. University of Puerto Rico-Mayagüez Campus, Dept. of Chemistry. 17. Recombinants of Mutant Human Centrin 2 (T169A/S170A) Noemí Soto-Nieves, Vivian

Negrón, B. Pastrana–Rios, Ph.D. University of Puerto Rico-Mayagüez, Department of Chemistry. 18. Over-Expression and Purification Of A Variant Human Centrin 2 Protein for

Biophysical Studies Upon Phosphorylation Victor Torres, B. Pastrana-Rios Chemistry Department, University of Puerto Rico Mayagüez Campus.

19. Use of Yeast Two-Hybrid System to Identify Protein-Protein Interactions of Heavy Chain Dynein with Mouse Centrin 1. Wanda Ocaña-Rivera†; German J. Lorenzo-Vargas†; James Orth§, Jeffrey L. Salisbury§, Belinda Pastrana-Rios† † University of Puerto Rico-Mayagüez Campus, Dept. of Chemistry and §Mayo Clinic and Foundation, Dept. of Biochemistry and Molecular Biology.

V. ACS-STM at Hotel El Faro in Aguadilla, PR on November 4, 2000

20. Bacterial Expression and Isolation of Human Centrin 1: A Flagella Associated Protein Mildred Ortiz, and Belinda Pastrana-Rios, Ph.D. Chemistry Department, University of Puerto Rico, Mayagüez PR

21. Bacterial Expression, Purification and Isolation of Human centrin 2 Wanda Ocaña Rivera1, Belinda Pastrana-Rios Ph.D. University of Puerto Rico-Mayagüez Campus,

VI. Sigma Xi at UPR-RUM on April 2001

22. Bacterial Expression of a Human centrin 2 mutant using pBAD/HIS Yasmine Valentin, Joel Otero, Belinda Pastrana-Rios, Ph.D. Chemistry Department, University of Puerto Rico, Mayagüez PR

VIII. ASCB National Meeting in Washington, DC on December 2001

23. Ca2+ and Mg2+ Binding Constants in Chlamydomonas Centrin. Wanda Ocaña Rivera1, Belinda Pastrana-Rios Ph.D.1, J.L. Salisbury Ph.D.2, Walter Chazin Ph.D.3, 1University of Puerto Rico-Mayagüez Campus, 2Mayo Clinic and Foundation; 3Vanderbilt University.

24. H/D Exchange FT-IR Spectroscopic Study of Chlamydomonas Centrin Domains Mildred Ortiz†, Belinda Pastrana, Ph.D. †, Haitao Hu, Ph.D. §, Walter Chazin, Ph.D. §, †Chemistry Department, University of Puerto Rico, Mayagüez PR §Center for Structural Biology, Vanderbilt University, Nashville, TN

25. Spectral Changes in Chlamydomonas Centrin in its unphosphorylated and Phosphorylated Forms Zuleika Sanoguet, Luis Perez-Moreno and Belinda Pastrana-Rios, Ph.D. Chemistry Department, University of Puerto Rico, Mayagüez PR

26. Expression of Chlamydomonas centrin monitored via FT-IR Spectroscopy Julianne del Toro, and Belinda Pastrana-Rios, Ph.D. Chemistry Department, University of Puerto Rico, Mayagüez PR

IX. ACS-STM at The Condado Plaza Hotel in San Juan, PR on November 2-3, 2001 27. Calcium Binding constants in Chlamydomonas Centrin W. Ocana-Rivera1, B. Pastrana-

Rios1, J. Salisbury3, W. Chazin2, 1Department of Chemistry University of Puerto Rico-Mayagüez Campus, 2Department of Structural Biology-Vanderbilt University, Nashville, TN, and 3Mayo Clinic & Foundation, Rochester, MN

28. H/D Exchange of model Peptides using ATR-FT-IR Spectroscopy M. Ortiz1, B. Pastrana1, W. Chazin2, H. Hu2 , 1Department of Chemistry University of Puerto Rico-Mayagüez Campus and 2Department of Structural Biology at Vanderbilt University, Nashville, TN

29. Spectral Changes in Chlamydomonas Centrin in its unphosphorylated and phosphorylated forms Zuleika Sanoguet, Luis Pérez-Moreno and Belinda Pastrana-Rios, Ph.D. Chemistry Department, University of Puerto Rico, Mayagüez PR.


X. Sigma Xi at UPR-RUM on April 2002 30. Expression and Isolation of Human centrin 2 mutant L171G/Y172G M. Silvestry, C.

Marrero, V. Negron, and B. Pastrana, Department of Chemistry, University of Puerto Rico, Mayagüez Campus

31. Recombinant Chlamydomonas centrin isolation and Purification by Affinity Chromatography I. Rodriguez, G. Roman, and B. Pastrana, Department of Chemistry, University of Puerto Rico, Mayagüez Campus

32. Attenuated Total Reflectance Fourier Transform Infrared (ATR-FT-IR) Spectroscopic study of Chlamydomonas centrin during Hydrogen-Deuterium Exchange M. Ortiz1, B. Pastrana1, W. Chazin2, H. Hu2, 1Department of Chemistry University of Puerto Rico-Mayagüez Campus and 2Department of Structural Biology-Vanderbilt University, Nashville, TN

33. Variant Human Centrin 2 (Hcen2) S170A Expression M. Vázquez-Coll, J. De Orbeta, V. Negrón and B. Pastrana-Rios, Department of Chemistry, University of Puerto Rico, Mayagüez Campus

34. Cloning of a mutant Human centrin 2 T169A/S170A protein E. Vázquez Zayas, B. Pastrana, Department of Chemistry, University of Puerto Rico, Mayagüez Campus

XI. 14th EPSCoR Annual Meeting on May 17-19, 2002 35. ATR/FT-IR Spectroscopic Study Of Chlamydomonas Centrin During H/D Exchange M.

Ortiz1, B. Pastrana1, W. Chazin2, H. Hu2, 1Department of Chemistry University of Puerto Rico-Mayagüez Campus and 2Department of Structural Biology-Vanderbilt University, Nashville, TN

36. Structural Changes in Chlamydomonas Centrin upon Ca2+ Titration W. Ocaña Rivera1, B. Pastrana-Rios1, J.L. Salisbury2, W. Chazin3, 1University of Puerto Rico-Mayagüez Campus, 2Mayo Clinic and Foundation, and 3Vandelbilt University

37. Thermal Perturbation of Phosphorylated Chlamydomonas centrin monitored via FT-IR spectroscopy Z. Sanoguet-Carrero and B. Pastrana-Rios, Department of Chemistry, University of Puerto Rico, Mayagüez Campus

XII. ACS-STM at The Caribe Hilton Hotel in San Juan, PR in August 6-7, 2002

38. Thermal Perturbation of Phosphorylated Chlamydomonas centrin via FT-IR spectroscopy Z. Sanoguet-Carrero and B. Pastrana-Rios, Department of Chemistry, University of Puerto Rico, Mayagüez Campus

XIII. BRIN at The Caribe Hilton Hotel in San Juan, PR in October 11-12, 2002 39. Generation of recombinant Human centrin 1 protein. Jessica De Orbeta and B. Pastrana-

Rios, Department of Chemistry, University of Puerto Rico, Mayagüez Campus XIX. 1st Colloquia on Protein Structure, Function and Dynamics, Theme: Protein Spectroscopy at the Hilton Hotel in Ponce, Puerto Rico in February 5-8, 2003.

40. TGF-ΑLPHA FAST H/D EXCHANGE STUDIES USING ATR-FT-IR Daniel Narváez1, Nancy Guillén1, Elsa Cora2, and Belinda Pastrana-Rios1, 1Department of Chemistry, University of Puerto Rico, Mayagüez Campus, 2Department of Biochemistry, University of Puerto Rico Medical Sciences

41. ATR/FT-IR Spectroscopic Study of Chlamydomonas Centrin During H/D Exchange Mildred Ortiz 1, Haitao Hu2, Walter Chazin2, and Belinda Pastrana-Rios1, 1Department of Chemistry, University of Puerto Rico, Mayagüez Campus, 2Center for Structural Biology, Vanderbilt University

42. CHARACTERIZATION OF PHOSPHORYLATED CHLAMYDOMONAS CENTRIN USING THERMAL PERTURBATION MONITORED VIA FT-IR SPECTROSCOPY AND MALDI-MS Zuleika Sanoguet Carrero and Belinda Pastrana-Ríos, Department of Chemistry, University of Puerto Rico, Mayagüez Campus

43. SELF-ASSOCIATION OF CHLAMYDOMONAS CENTRIN Wanda Ocaña1, Haitao Hu2, Walter Chazin2, and Belinda Pastrana-Rios1, 1Department of Chemistry, University of Puerto Rico, Mayagüez Campus, 2Center for Structural Biology, Vanderbilt University

44. LARGE SCALE EXPRESSION AND PURIFICATION OF NATIVE RECOMBINANT AND ISOTOPE EDITED CHLAMYDOMONAS CENTRIN Giselle Roman, Zuleika Sanoguet, Vanessa


Seiglie, and Belinda Pastrana-Ríos, Department of Chemistry, University of Puerto Rico Mayagüez Campus

XX. Alfred P. Sloan Conference at Mayagüez, PR, February 20-21, 2003

45. FAST H/D EXCHANGE TWO-DIMENSIONAL CORRELATION SPECTRAL STUDIES FOR EGF AND TGF- USING FT-IR SPECTROSCOPY Nancy Guillen, Daniel Narvaez, and Belinda Pastrana, Department of Chemistry, University of Puerto Rico Mayagüez Campus

46. GENERATION OF A RECOMBINANT OF HUMAN CENTRIN 2 Jessica Colón and Belinda Pastrana, Department of Chemistry, University of Puerto Rico Mayagüez Campus

XXI. Biophysical Meeting at San Antonio, Texas in March 1-3, 2003

47. FT-IR CHARACTERIZATION OF PHOSPHORYLATED CHLAMYDOMONAS CENTRIN Zuleika Sanoguet and Belinda Pastrana, Department of Chemistry, University of Puerto Rico, Mayagüez Campus

XXII. 38th ACS Junior Technical Meeting, San Juan Puerto Rico, March 8th, 2003

48. FAST H/D EXCHANGE TWO-DIMENSIONAL CORRELATION SPECTRAL STUDIES FOR EGF AND TGF- USING FT-IR SPECTROSCOPY Nancy Guillen, Daniel Narvaez, and Belinda Pastrana, Department of Chemistry, University of Puerto Rico Mayagüez Campus

49. LARGE SCALE EXPRESSION AND PURIFICATION OF RECOMBINANT CHLAMYDOMONAS CENTRIN (CCEN) AND ISOTOPE EDITED CCEN Gisselle Román, Zuleika Sanoguet, and Belinda Pastrana, Department of Chemistry, University of Puerto Rico Mayagüez Campus

50. RECOMBINANT OF HUMAN CENTRIN 2 Jessica Colón, Zuleika Sanoguet, and Belinda Pastrana, Department of Chemistry, University of Puerto Rico Mayagüez Campus

XXIII. Sigma Xi at UPR-RUM on April 2003 51. FERMENTOR EXPRESSION AND SUBSEQUENT ISOLATION OF 13C LABELED AND

NATIVE CHLAMYDOMONAS CENTRIN Gisselle Román, Mariena Silvestry, Michelle Vazquez, Zuleika Sanoguet, Vanessa Seiglie and Belinda Pastrana-Rios, Department of Chemistry, University of Puerto Rico Mayagüez Campus.

52. RECOMBINANT OF HUMAN PROTEIN: CENTRIN 2 Jessica Colón-Franco, Muriel Campbell Zuleika Sanoguet, and Belinda Pastrana, Department of Chemistry, University of Puerto Rico Mayagüez Campus

53. FAST EXCHANGE TWO-DIMENSIONAL CORRELATION SPECTRA STUDIES FOR EGF AND TGF- USING FT-IR Nancy Guillen, Daniel Narvaez, and Belinda Pastrana, Department of Chemistry, University of Puerto Rico Mayagüez Campus

XXIV. AMERICAN SOCIETY OF BIOCHEMISTRY AND MOLECULAR BIOLOGY (ASBMB): Proteomic Solution Cellular and Developmental Biology and Medicine, at Kansas City, Missouri, May 2003,

54. H/D EXCHANGE OF EGFR LIGANDS USING FT-IR SPECTROSCOPY AND 2D-CORRELATION ANALYSIS Nancy Guillen, Daniel Narvaez, and Belinda Pastrana, Department of Chemistry, University of Puerto Rico Mayagüez Campus

XXV. Summer Conference in Protein Research, at the Wyndham Hotel in old San Juan, Puerto Rico in August 2003. 55. EXPRESSION OF RECOMBINANT CALCIUM BINDING HUMAN PROTEIN Jessica De Orbeta,

Elmar Schiebel, and Belinda Pastrana-Rios, Department of Chemistry, University of Puerto Rico Mayagüez Campus

56. CONSTRUCCION OF RECOMBINANT CLONES OF A HUMAN CALCIUM BINDING PROTEIN Veronica Meza, José Fernández and Belinda Pastrana-Rios, Department of Chemistry, University of Puerto Rico Mayagüez Campus


57. FERMENTOR EXPRESSION AND SUBSEQUENT ISOLATION OF RECOMBINANT 13C LABELED AND NATIVE CHLAMYDOMONAS CENTRIN Michelle Vázquez, Gisselle Román, Mariena Silvestry, Zuleika Sanoguet and Belinda Pastrana-Rios, Department of Chemistry, University of Puerto Rico Mayagüez Campus.

58. LIGANDS OF GROWTH FACTOR RECEPTORS WITH TYROSINE KINASE ACTIVITY Nancy Guillen, Carlos Camacho, Daniel Narvaez, Elsa Cora and Belinda Pastrana, Department of Chemistry, University of Puerto Rico Mayagüez Campus and Department of Biochemistry, UPR-Medical Sciences

59. PHOSPHORYLATION PROVIDES AN ADDITIONAL MECHANISM FOR TARGET REGULATION BY CENTRIN Muriel Campbell, Walter Chazin and Belinda Pastrana, Department of Chemistry, University of Puerto Rico Mayagüez Campus and Vanderbilt University

60. STUDIES IN PHOSPHORYLATED RECOMBINANT CHLAMYDOMONAS CENTRIN Zuleika Sanoguet and Belinda Pastrana-Rios, Department of Chemistry, University of Puerto Rico Mayagüez Campus

XXVI. 43rd Annual Meeting of the American Society for cell Biology, San Francisco, California, December 13-17, 2003. 61. ERBB1 FAMILY OF LIGANDS FAST HYDROGEN/DEUTERIUM EXCHANGE KINETICS Daniel

Narvaez, Nancy Guillen, and Belinda Pastrana-Rios, Department of Chemistry, University of Puerto Rico Mayagüez Campus

62. EXPLAINING ENZYMATIC ACTIVITY THROUGH PHYLOGENY IN A CELL BIOLOGY COURSE Vélez A. and Pastrana-Rios, B. Department of Chemistry and Biology Dept., University of Puerto Rico Mayagüez Campus

XXVII. 48th Annual Meeting of the Biophyscal Society, Baltimore Maryland, February 14-18, 2004. 63. CHARACTERIZATION OF PHOSPHORYLATED RECOMBINANT CHLAMYDOMONAS

CENTRIN Zuleika Sanoguet, Muriel Campbell, Rebecca Lebrón and Belinda Pastrana, Department of Chemistry, University of Puerto Rico Mayagüez Campus

64. RECOMBINANT SOLUBLE EPIDERMAL GROWTH FACTOR RECEPTOR(EGFR) HYDROGEN/DEUTERIUM EXCHANGE STUDIES USING FT-IR SPECTROSCOPY Carlos Camacho, Daniel Narváez, Kathryn Ferguson, Belinda Pastrana Center of Protein Structure, Function, and Dynamics, Department of Chemistry, University of Puerto Rico, Mayagüez, Puerto Rico and The University of Pennsylvania Medical Sciences.

XXVIII. 39th ACS Junior Technical Meeting, Humacao, Puerto Rico, March 8th, 2004 65. CHARACTERIZATION OF PHOSPHORYLATED CHLAMYDOMONAS CENTRIN Muriel Campbell,

Z. Sanoguet, H. Hu, W. Chazin, B. Pastrana Center of Protein Structure, Function, and Dynamics, Department of Chemistry, University of Puerto Rico, Mayagüez, Puerto Rico and the Center for Structural Biology, Vanderbilt University, Nashville Tennessee.

66. STUDY OF EGFR LIGANDS USING FT-IR SPECTROSCOPY Nancy Guillen, Daniel Narváez, Carlos Camacho, Belinda Pastrana, Center of Protein Structure, Function, and Dynamics, Department of Chemistry, University of Puerto Rico, Mayagüez, Puerto Rico.

XXIX. Spring Conference in Protein Research, San Juan, PR, April 16th, 2004. 67. EVIDENCE OF AGGREGATION USING DIFFERENTIAL SCANNING CALORIMETRY FOR: A

CALCIUM BINDING PROTEIN CHLAMYDOMONAS CENTRIN, AND EGFR LIGANDS Muriel M. Campbell Ocasio, Zuleika Sanoguet, Daniel Narváez, Nancy Guillén, Jessica Colón, Haitao Hu, Walter Chazin, and Belinda Pastrana, Center of Protein Structure, Function, and Dynamics, Department of Chemistry, University of Puerto Rico, Mayagüez, Puerto Rico and the Center for Structural Biology, Vanderbilt University, Nashville Tennessee.

68. HYDROGEN/DEUTERIUM EXCHANGE STUDIES OF RECOMBINANT SOLUBLE EPIDERMAL GROWTH FACTOR RECEPTOR (SEGFR) Daniel Narvaez1 Carlos Camacho1, Kathryn M. Ferguson2, Belinda Pastrana-Rios1,3 , Center of Protein Structure, Function, and Dynamics, Chemistry, University of


Puerto Rico, Mayaguez, PR, USA, 2Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA

69. UNDERSTANDING THE EFFECT OF PHOSPHORYLATION IN RECOMBINANT CHLAMYDOMONAS CENTRIN Zuleika Sanoguet-Carrero2 Christina Seda2, Muriel Campbell2, Belinda Pastrana-Ríos1,2 1Center for Protein Structure, Function, and Dynamics,2Department of Chemistry, University of Puerto Rico, Mayagüez Campus.

70. EGFR FAMILY OF LIGANDS HYDROGEN/DEUTERIUM EXCHANGE Nancy Guillén2, Carlos Camacho2, Daniel Narváez2, Elsa Cora1,3 and Belinda Pastrana-Ríos1,2 Center for Protein Structure Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus, 3Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus.

XXX. CURRENT TRENDS IN BIOTECHNOLOGY & PHARMACEUTICAL ENGINEERING, Mayagüez,

PR, October 1, 2004. 71. TECHNIQUES SUITABLE FOR VERIFYING PHOSPHORYLATION OF CHLAMYDOMONAS

CENTRIN Christina Seda2, Muriel Campbell2, Kevin Weiss3, Walter Chazin3, and Belinda Pastrana-Ríos1,2 1Center for Protein Structure, Function, and Dynamics,2Department of Chemistry, University of Puerto Rico, Mayagüez Campus. 3Center for Structural Biology, Vanderbilt University, Nashville Tennessee.

72. ATTENUATED TOTAL REFLECTANCE FT-IR HYDROGEN/DEUTERIUM EXCHANGE OF RECOMBINANT ERBB1 RECEPTOR Daniel Narváez2, Carlos Camacho2, Kathryn M. Ferguson3 and Belinda Pastrana-Ríos1,2 Center for Protein Structure Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus, 3 Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA

73. EPIDERMAL GROWTH FACTOR AND TRANSFORMING GROWTH FACTOR-ALPHA HYDROGEN/DEUTERIUM EXCHANGE USING ATR-FT-IR SPECTROSCOPY Carlos Camacho2, Nancy Guillén2, Daniel Narváez2, Elsa Cora1,3 and Belinda Pastrana-Ríos1,2 Center for Protein Structure Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus, 3Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus.

74. FOURIER TRANSFORM INFRARED SPECTROSCOPY AND MALDI-MASS SPECTROMETRY OF PHOSPHORYLATED CHLAMYDOMONAS CENTRIN Zuleika Sanoguet-Carrero2 Christina Seda2, Muriel Campbell2, R. Lebrón2, Belinda Pastrana-Ríos1,2 1Center for Protein Structure, Function, and Dynamics,2Department of Chemistry, University of Puerto Rico, Mayagüez Campus.

75. STRUCTURAL CHANGES IN 13C-CENTRIN AND MELLITIN UPON BINDING L. del Valle Sosa2 and Belinda Pastrana-Ríos1,2 1Center for Protein Structure, Function, and Dynamics,2Department of Chemistry, University of Puerto Rico, Mayagüez Campus.

76. MOLECULAR DYNAMICS OF SURFACTANT PROTEIN C IN A HYDROPHILIC MEDIA E. Rámirez2, I. Plasencia2, B. Pastrana-Ríos1,2 and G. López1,2 1Center for Protein Structure, Function, and Dynamics,2Department of Chemistry, University of Puerto Rico, Mayagüez Campus.

XXXI. AACR Meeting Fort Lauderdale, Florida, December 5-12, 2004. 77. RELATIVE THERMAL STABLITY OF PHOSPHORYLATED AND UNPHOSPHORYLATED

CHLAMYDOMONAS CENTRIN Z. Sanoguet-Carrero1, C. Seda1, B. Pastrana-Rios1,2; 1Chemistry, University of Puerto Rico, Mayagüez, PR, 2Center for Protein Structure Function

78. CALORIMETRIC STUDIES OF A CENTROSOMAL ASSOCIATED PROTEIN: CENTRIN M. M. Campbell-Ocasio2, Z. Sanoguet-Carrero2, W. Chazin3 and B. Pastrana-Rios1,2 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayaguez, Puerto Rico and 3Center for Structural Biology, Vanderbilt University, Nashville Tennessee.

XXXII. 44th Annual Meeting of the American Society for Cell Biology, Washington, DC, December 2004 79. THERMAL DEPENDENCE STUDIES OF EPIDERMAL GROWTH FACTOR RECEPTOR LIGANDS

Daniel Narvaez2, Carlos Camacho2, Nancy Guillén2, Elsa Cora1,3 and Belinda Pastrana-Ríos1,2. 1Center for


Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus. 3Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus

XXXIII. 2nd Colloquium in Protein Structure, Function and Dynamics, San Juan, Puerto Rico

February 15-19, 2005 80. HIGH LEVEL EXPRESSION AND THERMAL CHARACTERIZATION OF HUMAN CENTRIN 1

Jessica De Orbeta2, Muriel Campbell2, Christina Seda and Belinda Pastrana-Rios1,2 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus PO Box 9019 Mayagüez, PR 00681.

81. H/D EXCHANGE DYNAMICS AND KINETICS OF ERBB FAMILY OF LIGANDS Ibon Iloro2, Daniel Narváez2, Carlos M. Camacho2, Elsa Cora1,3 and Belinda Pastrana-Rios1,2 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus. 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus. PO Box 9019, Mayagüez, Puerto Rico 00681-9019 3Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus. PO Box 365067, San Juan, Puerto Rico 00936-5076.

82. PRELIMINARY CRYSTALLIZATION OF CHLAMYDOMONAS CENTRIN Alice Hernández Castillo2, Belinda Pastrana-Rios1,2 and Jorge Rios Steiner1,2 1Center for Protein Structure, Function and Dynamics, 2Chemistry, University of Puerto Rico, Mayagüez Campus, PO Box 9019 Mayagüez, PR 00681

83. THE INTERACTION OF CHLAMYDOMONAS CENTRIN 13C LABEL WITH MELLITIN A ANPHIPHILIC PEPTIDE Liliana del V. Sosa2 and Belinda Pastrana-Rios1,2 1 Center for Protein Function, Structure and Dynamics, 2Department of Chemistry, University of Puerto Rico Mayagüez Campus, PO Box 9019 Mayagüez, Puerto Rico 00681

84. SURFACTANT PROTEIN C ANALOGUES AS EXOGENOUS THERAPEUTICS FOR SEVERE RESPIRATORY DISORDERS Sindia Ramos2, Muriel Campbell2, Gustavo López1,2, and Belinda Pastrana-Rios1,21Center for Research in Protein Structure, Function and Dynamics, 2Chemistry, University of Puerto Rico, Mayagüez Campus

85. EXPRESSION, PURIFICATION AND CHARACTERIZATION OF A HUMAN CALCIUM BINDING PROTEIN: HUMAN CENTRIN 2 Verónica Meza-Venencia2, Muriel Campbell2 and Belinda Pastrana-Ríos1,2 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

86. RELATIVE THERMAL STABILITY AND DYNAMICS OF PHOSPHORYLATED AND UNPHOSPHORYLATED CHLAMYDOMONAS CENTRIN Zuleika Sanoguet Carrero2, Muriel Campbell2 and Belinda Pastrana-Ríos1,2 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

XXXIV. Beta Beta Beta Regional Chapter National Convention 2005, University of Puerto Rico, Mayaguez Campus 87. TECHNIQUES SUITABLE FOR VERIFYING PHOSPHORYLATION OF C-TERMINAL DOMAIN

OF CHLAMYDOMONAS CENTRIN Christina Seda and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus.

XXXV. 19th Symposium of the Protein Society, Boston, MA July 30 - August 3rd, 2005 88. INFRARED AND CALORIMETRIC STUDIES OF SURFACTANT PROTEIN C ANALOGUES

Sindia Ramos2, Gustavo López1,2 and Belinda Pastrana-Rios1,2 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus.

89. CHLAMYDOMONAS 13C-CENTRIN AND MELLITIN INTERACTIONS Liliana Sosa2 and Belinda Pastrana-Ríos1,2 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

90. EVIDENCE OF SELF ASSOCIATION AND RELATIVE THERMAL STABILITY OF PHOSPHORYLATED AND UNPHOSPHORYLATED CENTRIN Muriel Campbell2, Zuleika Sanoguet Carrero2, and Belinda Pastrana-Ríos1,2 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus.


91. STRUCTURAL DIFFERENCES UPON PHOSPHORYLATION OF CHLAMYDOMONAS CENTRIN Christina Seda2, Muriel Campbell2 Keivin Weiss3, Walter Chazin3 and Belinda Pastrana-Ríos1,2 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus and 3Center for Structural Biology, Vanderbilt University, Nashville Tennessee.

XXXV. 19th Symposium of the Protein Society, Boston, MA July 30 - August 3rd, 2005 (Cont.) 92. DYNAMICS OF H/D EXCHANGE IN EGF, TGF-ALPHA AND HEPARIN-BINDING EGF: A

2DCOS STUDY Ibon Iloro1,2, Daniel Narváez2, Carlos M. Camacho2, Elsa Cora1,3 and Belinda Pastrana-Rios1,2 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus. 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus. PO Box 9019, Mayagüez, Puerto Rico 00681-9019 3Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus. PO Box 365067, San Juan, Puerto Rico 00936-5076.

93. DYNAMICS OF EPIDERMAL GROWTH FACTOR RECEPTOR LIGANDS DURING THERMAL DENATURION Daniel Narváez2, Carlos M. Camacho2 Nancy Guillen2, Elsa Cora1,3 and Belinda Pastrana-Rios1,2 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus. 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus. PO Box 9019, Mayagüez, Puerto Rico 00681-9019 3Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus. PO Box 365067, San Juan, Puerto Rico 00936-5076.

94. MOLECULAR DYNAMICS OF SURFACTANT PROTEIN C IN A HYDROPHILLIC MEDIA Gustavo López, Eunice Ramirez, Alberto Santana, Antony Cruz Belinda Pastrana and Inés Plascencia Department of Chemistry, University of Puerto Rico, Mayagüez Campus. PO Box 9019, Mayagüez,

XXXVI. Vanderbilt University Summer Science Academy Symposium, August 5th in Nashville Tennessee 95. THE EFFECT OF PHOSPHORYLATION ON STRUCTURE AND FUNCTION OF C-TERMINAL

DOMAIN OF CENTRIN. Christina Seda, Belinda Pastrana and Walter Chazin 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus and 3Center for Structural Biology, Vanderbilt University, Nashville Tennessee.

XXXVII. 3rd International Symposium on Two-dimensional Correlation Analysis, Delavan, WI August 12-14, 2005. 96. DYNAMICS OF H/D EXCHANGE IN EGF, TGF-ALPHA AND HEPARIN-BINDING EGF: A

2DCOS STUDY Ibon Iloro1,2, Daniel Narváez2, Carlos M. Camacho2, Elsa Cora1,3 and Belinda Pastrana-Rios1,2 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus. 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus. 3Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus.

XXXVIII. 45th Annual Meeting of the American Society for Cell Biology, San Francisco, CA December 2005 97. CHARACTERIZATION OF CENTRIN AN MTOC’S PROTEIN Sanoguet, Z., Campbell, M.,

Pastrana-Rios, B. 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus 2005 Molec. Biol. Cell 12, 27a.

98. CALORIMETRIC PROPERTIES OF A CENTROSOME ASSOCIATED PROTEIN: HUMAN CENTRIN 2 Meza-Venencia, V., Pastrana-Ríos, B. 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus 2005 Molec. Biol. Cell 12, 27a.

XXXIV. Spring Conference in Protein Research, San Juan, PR, March 2-5th, 2005. 99. X-RAY CRYSTAL STRUCTURE OF CHLAMYDOMONAS REINHARDTII CENTRIN

Elisa C. Alfaro, Eric R. Schreiter, Belinda Pastrana-Ríos 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

100. EXPRESSION, ISOLATION AND PURIFICATION OF HCEN 1 VARIANTS Joel Díaz, Arnaldo Zayas, Abigail Padilla, and Ana María Gomez, Belinda Pastrana 1Center for Protein


Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

101. SIMULATION OF FT-IR SPECTRA AND 2D-COS ANALYSIS FOR THE H/D EXCHANGE OF TWO RELATED LIGANDS Ibon Iloro and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

102. H/D EXCHANGE OF SEGFR AND 13C-LABELED EGF EXPRESSION AND ISOLATION Daniel Narváez, Ibon Iloro, Melissa Serrano, Kathryn M. Ferguson, Elsa Cora and Belinda Pastrana-Rios

103. SURFACTANT PROTEIN C ANALOGUES AND MODEL LIPID INTERACTIONS IN LUNG SURFACTANT Sindia Ramos, Gustavo López, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

104. DYNAMICS OF A HUMAN CALCIUM BINDING PROTEINS: Centrin 1 Myrna Reyes, Belinda Pastrana-Ríos 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

105. THE EFFECT OF PHOSPHORYLATION ON STRUCTURE AND FUNCTION OF C-TERMINAL DOMAIN OF CENTRIN Christina Seda and Muriel Campbell, Susan Meyn, Kevin Weiss, Belinda Pastrana and Walter Chazin 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus and 3Center for Structural Biology, Vanderbilt University, Nashville Tennessee.

106. THERMAL DEPENDENCE STUDIES IN CHLAMYDOMONAS 13C-CENTRIN AND Sfi1p COMPLEX Liliana del V. Sosa and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

XXXX. EXPO CHEM, Mayaguez PR, August 2006 107. TGF- AND EGF Daniel Narváez, Nancy Guillen, Carlos Camacho, Ibon Iloro Lalisse Guillen, Elsa

Cora, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus. 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus. 3Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus.

108. H/D EXCHANGE OF sEGFR And 13C-LABELED EGF EXPRESSION AND ISOLATION Daniel Narváez, Ibon Iloro Melissa Serrano, Kathryn Fergusson, Elsa Cora and Belinda Pastrana-Rios Center for Protein Structure Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus, 3 Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA; 4Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus.

109. LARGE SCALE EXPRESSION AND PURIFICATION OF RECOMBINANT CCEN-C AND ISOTOPE EDITED HCEN1 Raquel Nunez, Lalisse Guillen, Zuleika Sanoguet, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

110. PROTEIN EXPRESSION AND ISOLATION OF RECOMBINANT HUMAN SFI1 DOMAINS 1 AND 2 Zuleika Sanoguet, Héctor Mártir, Joel Díaz, Cristina Luiggi and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

111. CHARACTERIZATION OF CENTRIN: AN MTOC PROTEIN Zuleika Sanoguet, Christina Seda Muriel Campbell, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

112. EXPRESSION, ISOLATION AND PURIFICATION OF HCEN1 VARIANTS Joel Díaz, Zuleika Sanoguet, Ana María Gómez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

113. CLONNING OF HUMAN SFI1 CENTRIN-BINDING DOMAINS AND MUTANTS OF CENTRIN INTO PROTEIN EXPRESSION VECTORS Ana María Gómez, Nidia Muñoz, Jessica Rios, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

114. EXPRESSION, ISOLATION AND PURIFICATION OF HCEN1 FOR ITS CRYSTALLIZATION Cristina López Fagundo, Frances Vales Lara, Elisa Alfaro Malavé Eric Schreiter, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus


XLI. PRISM, Bayamon, PR, November, 2007 115. ISOLATION AND PURIFICATION OF 13C LABELED HUMAN CENTRIN 1 Jorge Santiago,

Zuleika Sanoguet-Carrero, Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

116. THE ISOLATION, PURIFICATION AND CHARACTERZATION OF VARIANT HUMAN CENTRIN Katia Ortiz, Zuleika Sanoguet-Carrero, Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

XLII. SIGMA XI, Mayaguez, PR, April 26, 2007 117. EXPRESSION OF HUMAN PROTEIN HCEN1 (E105K) Norma I. Rodriguez-Malavé, Ana María

Gómez, Zuleika Sanoguet, Daniel Narváez and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

118. PURIFICATION OF A HUMAN PROTEIN HCEN1 (E105K) Verónica López, Daniel Narváez, Zuleika Sanoguet, Ana María Gómez and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

XLIII. American Institute of Chemical Engineers (AIChE) National Convention, Utah,

Nov. 2007 119. CRYSTALLIZATION OF CENTRIN COMPLEXES Quiles, O.X. and B. Pastrana-Rios 1Center for

Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

XLIV. PRISM, Arecibo, PR, November, 2008 120. PROTEIN EXPRESSION AND ISOLATION OF RECOMBINANT HUMAN SFI1 Santiago-Ortiz,

J.L., Z. Sanoguet-Carrero, B. Pastrana-Rios, Center for Protein Structure, Function and Dynamics, & Department of Chemistry, University of Puerto Rico, Mayagüez Campus

XLV. 28th Latin American Congress in Chemistry San Juan, PR July 27 – Aug. 1st, 2008 121. THE KINETICS OF THE HYDROGEN/DEUTERIUM EXCHANGE OF EPIDERMAL GROWTH

FACTOR RECEPTOR LIGANDS Ibon Iloro, Daniel Narvaez, Nancy Guillen, Carlos M. Camacho, Lalisse Guillen, Elsa Cora and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus. 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus. 3Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus.

122. CLONING OF HUMAN SFI1 CENTRIN-BINDING DOMAINS INTO PROTEIN EXPRESSION VECTORS Ana María Gómez and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

123. CLONING AND EXPRESSION OF THE FULL-LENGTH HUMAN SFI1 PROTEIN Melody M. López, Ana María Gómez and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

124. PROTEIN CO-EXPRESSION OF HUMAN CENTRIN 2 AND HUMAN SFI1 BINDING DOMAINS Katia Ortiz Lugo and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

125. EXPRESSION, PURIFICATION, AND CHARACTERIZATION OF HUMAN CENTRIN 1 VARIANTS Joel E. Diaz-Arana, Ana Maria Gomez, Zuleika Sanoguet, Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, 2Department of Chemistry, University of Puerto Rico, Mayagüez Campus

XLVI. 2009 National 2DCOS Symposium, Rincón, PR February 20-22, 2009

126. THE KINETICS OF THE HYDROGEN/DEUTERIUM EXCHANGE OF EPIDERMAL GROWTH FACTOR RECEPTOR LIGANDS Ibon Iloro, Daniel Narvaez, Nancy Guillen, Carlos M. Camacho, Lalisse Guillen, Elsa Cora and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus. 2Department of Chemistry, University of


Puerto Rico, Mayagüez Campus. 3Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus.

127. 2DCOS ANALYSIS OF A HUMAN CENTRIN 2 VARIANT BY FT-IR SPECTROSCOPY Jorge Santiago, Daniel Narvaez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.

128. FT-IR SPECTRA AND 2DCOS ANALYSIS FOR THE THERMAL PERTURBATION OF HUMAN CENTRIN 2 Nilsa Méndez, Oscar X. Quiles, Pablo Acosta, Zully A. Rivera, Melissa Ortiz, Ana M. Gómez, Daniel Narvaez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.

129. CONFORMATIONAL STABILITY OF HCEN1(E105K) UPON THERMAL PERTURBATION USING 2DCOS Amalchi Castillo, Roberto I. Pares, Nicole Martinez, Ana M. Gómez, Daniel Narvaez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.

XLVII. XVI SIGMA XI Mayagüez, PR April 12, 2011

130. CLONING AND LARGE SCALE EXPRESSION OF A NOVEL CENTRIN TARGET PROTEIN Ruth Almodovar, Ana M. Gómez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.

131. ISOLATION AND PURIFICATION OF HOMO SAPIENS CENTRIN 2 VARIANT PROTEIN Lizbeth Irizarry, Mariely Quiñones, Ana M. Ortiz, Daniel Narváez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.

132. CLONING AND EXPRESSION OF THE N-TERMINAL DOMAIN OF A CALCIUM BINDING PROTEIN Brenda I. Méndez, Ana M. Gómez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.

133. BACTERIAL EXPRESSION AND ISOLATION OF NOVEL INDEL CANDIDATES Pedro Ondina, Ana M. Gómez, Taras Oleksyk, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.

134. CLONING OF THE N-TERMINAL DOMAIN OF A CENTRIN GENE Pedro Ramos, Ana M. Gómez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus

135. ISOLATION AND PURIFICATION OF A NOVEL CENTRIN TARGET PEPTIDE José Robles, Daniel Narváez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.

136. CLONING OF A NOVEL CENTRIN TARGET PEPTIDE Aslin Rodriguez, Ana M. Gómez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.

137. EXPRESSION AND PURIFICATION OF A CENTRIOLE ASSOCIATED PEPTIDE Marie Cely Rosado, Daniel Narváez, Ana María Gómez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.

XLVIII. Eli Lilly Technical Forum, Carolina PR April 15, 2011

138. EXPRESSION OF FULL-LENGTH HUMAN KRR1, A PUTATIVE BIOLOGICAL TARGET OF CENTRIN, USING E. coli CELLS Ruth Almodovar, Ana M. Gómez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.

139. PURIFICATION OF HUMAN CENTRIN2 (F5W) USING HYDROPHOBICITY AND ANION EXCHANGE CHROMATOGRAPHY Lizbeth Irizarry, Mariely Quiñones, Ana M. Ortiz, Daniel Narváez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.

140. CLONING AND EXPRESSION OF THE N-TERMINAL HOMO sapiens CENTRIN2 IN E. coli CELLS Brenda I. Méndez, Ana M. Gómez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.


141. CLONING OF NOVEL INDEL CANDIDATES AND THEIR BACTERIAL EXPRESSION AND ISOLATION Pedro Ondina, José Montoya, Ana M. Gómez, Taras Oleksyk, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.

142. CLONING OF THE N-TERMINAL FRAGMENT OF CHLAMYDOMONAS reinhardtii CENTRIN GENE Pedro Ramos, Ana M. Gómez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus

143. PURIFICATION OF PRP40 FRAGMENT VIA SUCCESSIVE COLUMN CHROMATOGRAPHIES José Robles, Daniel Narváez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.

144. CLONING AND PROTEIN EXPRESSION OF THE KRR1-KH DOMAIN Aslin Rodríguez, Ana M. Gómez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.

145. EXPRESSION, PURIFICATION AND CHARACTERIZATION OF RECOMBINANT HIS-TAGGED HsSFI1p1-2 PEPTIDE Marie Cely Rosado, Daniel Narváez, Ana María Gómez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.

XLIX. 46th IUPAC San Juan, Puerto Rico July31-August 4th,2011 146. CLONING AND PROTEIN EXPRESSION OF THE KRR1-KH DOMAIN Aslin Rodriguez, Ana M.

Gómez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.

147. SITE-DIRECTED MUTAGENESIS AND EXPRESSION OF HOMO SAPIENS CENTRIN 1 AND 2 Lorraine Hernández, Ana M. Gómez, and Belinda Pastrana-Rios 1Center for Protein Structure, Function and Dynamics, University of Puerto Rico, Mayagüez Campus.

L. AAAS Mayagüez, Puerto Rico October 1st, 2011 148. NOVEL TARGET PROTEIN FOR CENTRIN Aslin Rodriguez, Ana María Gómez, and Belinda

Pastrana, University of Puerto Rico, Mayagüez Campus.

LI. Caribbean GMP, Mayagüez, Puerto Rico November 7-9, 2011 149. CLONNING AND BACTERIAL EXPRESSION OF HOMO SAPIENS CENTRIN Brenda Ramos,

Ana María Gómez, and Belinda Pastrana, University of Puerto Rico, Mayagüez Campus. 150. KRR1 A NOVEL BIOLOGICAL TARGET FOR CENTRIN Aslin Rodriguez, Ana Maria Gómez, and

Belinda Pastrana, University of Puerto Rico, Mayagüez Campus.

LII. Biomedical Engineering Semi Annual Meeting, Mayagüez, Puerto Rico, November 5th, 2011 151. ISOLATION OF CALCIUM BINDING PROTEINS AND ITS INETRACTING PEPTIDES USING

T7 PHAGE DISPLAY Eric J. Santiago Chaparro, Odemaris Narváez del Pilar, Aslin Rodriguez, Ruth Almodovar, Belinda Pastrana and Carlos Ríos Velázquez, University of Puerto Rico, Mayagüez Campus.

JUNIOR HIGH AND HIGH SCHOOL LEVEL RESEARCH PROJECT PRESENTATIONS AND COMPETITIONS

1. SESO Regional Science Fair January 11, 2003, Mayaguez, PR Jessica Flores (1st place Chemistry) and Belinda Pastrana

2. SESO Regional Science Fair February 15, 2005, Mayaguez, PR IN SITU EXPERIMENTS IN THE LUNG Roberto Rios (1st place Physics) and Belinda Pastrana

3. Discovery Channel Young Scientist Challenge October 15-20, 2005; Washington, DC IN SITU EXPERIMENTS IN THE LUNG Roberto Rios (finalist) and Belinda Pastrana


4. SESO Regional Science Fair February 13, 2007, Mayaguez, PR A LETHAL PROTEIN MUTATION Roberto Rios (1st place Biochemistry) and Belinda Pastrana 5. INTEL International Science and Engineering Fair (ISEF) May 13-18, 2007; Albaquerque, NM

Roberto Rios (observer) 6. SESO Regional Science Fair February 2, 2008, Mayaguez, PR EXPLORING THE INTERACTION IN A CENTRIN-SFI1P COMPLEX Roberto Rios (Overall Outstanding and 1st place Biochemistry) and Belinda Pastrana 7. INTEL International Science and Engineering Fair (ISEF) May 11-16, 2008; Atlanta, GA EXPLORING THE INTERACTION IN A CENTRIN-SFI1P COMPLEX Roberto Rios (finalist) and Belinda Pastrana

Vitae BELINDA PASTRANA-RIOS - UPRMacademic.uprm.edu/~pastranaslab/docs/bpcv_2011d.pdf · Vitae...

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