Visualization of prostate fibromuscular stromal matrix ... · nd 1.6 ± 0.9 mm ful to recover th...
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1 Centr Sydn Introd enviro voxel b x 10 -3 observ Our pr of 0.47 spatia optim Mater immer spin-e directio Prior to spaces recons deflect Resul encod values At b = fibertra metho stroma Figure (Row 2 the str Concl range the FA muscle resolu Refere 10.100 Septem Visuali re for Advanced Im ey, Sydney, New S Australia, 4 D duction: 16.4T h nments and tissu bulk mean diffus mm 2 /sec. It was ved in vivo may b reliminary data s 7-0.66, which is h l resolution and t ize the methodo ials & Methods: rsed in 0.2% Mag cho DTI sequenc ons and 2 b = 0 o processing the s. Diffusion para structions were a tion, and stream ts DTI tractograp ed FA images. T s started to increa 2000 s/mm 2 , FA acks (1.0 ± 0.7 a ods appeared use al tissues. e 1: DTI tractogra 2,3,4) Fibertrack reamlines that int usion: In formal 1000-2000 s/mm ACT algorithm. C e fiber orientation tion in vivo. ences: (1) Bourn 02/mrm.22778. ( mber 2011. (3) R ization of pros Nyo maging, University South Wales, Austr Discipline of Medic igh-resolution dif ue architecture c ivity, D = 0.54 ± suggested that t be the result of a how that the fibro higher compared tissue intrinsic an ology for the vis : A 3 mm diamet gnevist. MRI was ce at 80μm isotro images. e whole dataset, metric images w also performed u line interpolation phy produces str The longest and m ase from a stable ACT algorithm pro and 1.6 ± 0.9 mm eful to recover th aphy reconstruct ks reconstructed tersect the displa lin-fixed prostate m 2 . The 2 nd orde Correlation with s n. These high-re ne R, et al. 16 T 2) Bourne R et a Ruopeng W & We state fibromus oman D. Kurniawa y of Queensland, B ralia, 3 Department cal Radiation Scien ffusion weighted consistent with th 0.05 x 10 -3 mm 2 / the negative corr an increase of pa omuscular strom d to the epithelium nisotropy will per sualization of fi ter normal prosta s performed on a opic resolution w a tissue mask wa were calculated u sing non-conven n (IS) [3]. reamlines that co most dense fiber e value, which is oduced 4200 ± 1 m, respectively) a he fibertracks at h tion of a normal p with FACT, 2 nd o ayed slice are sh tissue, the most r Runge-Kutta a serial section hist esolution ex-vivo diffusion microim al. Microscopic D edeen VJ, Track scular stromal an 1 , Gary Cowin 1 , Brisbane, Queensla t of Anatomical Pa nces, Faculty of He imaging (DWI) o hat seen on light /sec. The diffusio relation between artial volume of e mal tissue exhibits m-containing vox rmit reliable 3D re bromuscular st ate tissue sample a 16.4T Bruker M with δ/∆ = 2/12 m as applied to rem sing DiffusionTo ntional algorithms orrelate with the o rtracks were deri s probably due to 100 fibertracks w nd high number high b-value (b ≥ prostate tissue s order Runge-Kutt hown. t dense and long nd Interpolated S topathology will b o studies will infor maging of fixed p Diffusivity Compa kVis.org, Proceed l matrix using Paul Sved 2 , Geoff and, Australia, 2 D athology, Royal Pr ealth Sciences, Th of formalin-fixed microscopy [1]. T on in the fibromu measured appa pithelial tissue an s microscopic dif xels with mean F econstruction of tromal matrix us e was collected fr Microimaging (5 m s, b=120, 250, 5 move the buffer s olkit 0.5 using th s: 2 nd order Rung orientation of fibr ved from data ac o an increase of t with an average o of fibertracks (96 ≥ 5000 s/mm 2 ), ho ample acquired ta and Interpolate gest stromal tissu Streamline propa be required to co rm attempts to m rostate tissue: pr rtmentation in Fo dings of ISMRM g ex-vivo high- fery Watson 3 , and Department of Urol rince Alfred Hospi he University of Sy prostate tissue h The epithelial ce scular stromal m arent diffusion co nd associated de ffusion anisotrop FA range 0.31-0.5 the fibromuscula sing the fiber tr rom a formalin fix mm solenoid RF 500, 1000 2000, signals from outs he conventional f ge Kutta (RK), wh romuscular strom cquired using 10 the noise level. of 0.6 ± 0.5 mm lo 650 ± 250 and 13 owever they also at multiple b valu ed Streamlines a ue fiber tracks ar agation algorithm onfirm that the ge measure fractiona reliminary finding ormalin-Fixed Pr 15, 2007; 3720. -resolution DT Roger Bourne 4 logy, Royal Prince ital, University of S ydney, Sydney, New has revealed dist ell layer has highl matrix is relatively efficient (ADC) a ecrease of strom py with the mean 54. We hypothes ar stromal structu racks calculated xed radical prost coil, Micro2.5 gr 5000 and 10000 side the tissue wh fiber propagation hich propagates mal anisotropy sh 000 ≤ b ≤ 2000 s/ ong. The RK and 3952 ± 468, resp o produced more ues. (Row 1) Dire algorithms, respe re generated from ms produced mor enerated fiber tra al anisotropy of p gs. Magn Reson rostate Tissue. M TI tractograph e Alfred Hospital, Sydney, Sydney, N w South Wales, Au tinct microscopic ly restricted diffu y less restricted, and cancer Gleas mal tissue and du fractional anisot size that a combi ures. This study d from DTI-tract tatectomy specim adient set: 2.5 G 0 s/mm 2 , 6 non-co hile keeping the n algorithm FACT the fiber tracks u hown by the dire /mm 2 . At b ≥ 500 d IS algorithms p pectively). The R e false streamline ectional color-en ectively. To aid v m DTI data with b re number and lo acks accurately r prostate tissue at Med. 2011 doi: Magn Reson Med hy The University of New South Wales, ustralia c diffusion sion with the D = 0.91 ± 0.17 son grade ctal space [2]. tropy (FA) range ination of high- y aims to tography. men and G/cm/A) using 3D ollinear sample ductal T. Additional using tensor ctional color- 00 s/mm 2 , the FA produced longer RK and IS es into non- ncoded FA map. visualization, only b-values in the onger tracks than reflect stromal t low spatial d. Accepted 24 D y n 1515 Proc. Intl. Soc. Mag. Reson. Med. 20 (2012)
Transcript of Visualization of prostate fibromuscular stromal matrix ... · nd 1.6 ± 0.9 mm ful to recover th...
1CentrSydn
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Materimmerspin-edirectioPrior tospacesreconsdeflect
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re for Advanced Imey, Sydney, New S
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duction: 16.4T hnments and tissubulk mean diffusmm2/sec. It was ved in vivo may breliminary data s7-0.66, which is hl resolution and tize the methodo
ials & Methods:rsed in 0.2% Magcho DTI sequencons and 2 b = 0 o processing thes. Diffusion parastructions were ation, and stream
ts DTI tractograped FA images. T
s started to increa2000 s/mm2, FA
acks (1.0 ± 0.7 aods appeared useal tissues.
e 1: DTI tractogra2,3,4) Fibertrackreamlines that int
usion: In formal1000-2000 s/mm
ACT algorithm. Ce fiber orientationtion in vivo.
ences: (1) Bourn02/mrm.22778. (mber 2011. (3) R
ization of prosNyo
maging, UniversitySouth Wales, AustrDiscipline of Medic
igh-resolution difue architecture civity, D = 0.54 ± suggested that t
be the result of ahow that the fibrohigher comparedtissue intrinsic anology for the vis
: A 3 mm diametgnevist. MRI wasce at 80μm isotroimages.
e whole dataset, metric images w
also performed uline interpolation
phy produces strThe longest and mase from a stable
ACT algorithm proand 1.6 ± 0.9 mmeful to recover th
aphy reconstructks reconstructed tersect the displa
lin-fixed prostatem2. The 2nd ordeCorrelation with sn. These high-re
ne R, et al. 16 T 2) Bourne R et a
Ruopeng W & We
state fibromusoman D. Kurniaway of Queensland, Bralia, 3Departmentcal Radiation Scien
ffusion weighted consistent with th0.05 x 10-3 mm2/the negative corr
an increase of paomuscular stromd to the epitheliumnisotropy will persualization of fi
ter normal prostas performed on aopic resolution w
a tissue mask wawere calculated u
sing non-convenn (IS) [3].
reamlines that comost dense fibere value, which isoduced 4200 ± 1
m, respectively) ahe fibertracks at h
tion of a normal pwith FACT, 2nd oayed slice are sh
tissue, the mostr Runge-Kutta a
serial section histesolution ex-vivo
diffusion microimal. Microscopic Dedeen VJ, Track
scular stromalan1, Gary Cowin1, Brisbane, Queenslat of Anatomical Pances, Faculty of He
imaging (DWI) ohat seen on light /sec. The diffusiorelation between
artial volume of emal tissue exhibitsm-containing voxrmit reliable 3D reibromuscular st
ate tissue samplea 16.4T Bruker Mwith δ/∆ = 2/12 m
as applied to remsing DiffusionTo
ntional algorithms
orrelate with the ortracks were deris probably due to100 fibertracks wnd high number high b-value (b ≥
prostate tissue sorder Runge-Kutthown.
t dense and longnd Interpolated Stopathology will b
o studies will infor
maging of fixed pDiffusivity CompakVis.org, Proceed
l matrix usingPaul Sved2, Geoffand, Australia, 2D
athology, Royal PrHealth Sciences, Th
of formalin-fixed microscopy [1]. T
on in the fibromumeasured appa
pithelial tissue ans microscopic difxels with mean Feconstruction of tromal matrix us
e was collected frMicroimaging (5 m
s, b=120, 250, 5
move the buffer solkit 0.5 using ths: 2nd order Rung
orientation of fibrved from data ac
o an increase of twith an average o
of fibertracks (96≥ 5000 s/mm2), ho
ample acquired ta and Interpolate
gest stromal tissuStreamline propabe required to corm attempts to m
rostate tissue: prrtmentation in Fodings of ISMRM
g ex-vivo high-fery Watson3, and
Department of Urolrince Alfred Hospihe University of Sy
prostate tissue hThe epithelial cescular stromal m
arent diffusion cond associated deffusion anisotrop
FA range 0.31-0.5the fibromuscula
sing the fiber tr
rom a formalin fixmm solenoid RF 500, 1000 2000,
signals from outshe conventional fge Kutta (RK), wh
romuscular stromcquired using 10the noise level. of 0.6 ± 0.5 mm lo650 ± 250 and 13owever they also
at multiple b valued Streamlines a
ue fiber tracks aragation algorithmonfirm that the ge
measure fractiona
reliminary findingormalin-Fixed Pr15, 2007; 3720.
-resolution DTRoger Bourne4
logy, Royal Princeital, University of Sydney, Sydney, New
has revealed distell layer has highlmatrix is relatively
efficient (ADC) aecrease of strom
py with the mean 54. We hypothesar stromal structuracks calculated
xed radical prostcoil, Micro2.5 gr5000 and 10000
side the tissue whfiber propagationhich propagates
mal anisotropy sh000 ≤ b ≤ 2000 s/
ong. The RK and3952 ± 468, respo produced more
ues. (Row 1) Direalgorithms, respe
re generated fromms produced morenerated fiber traal anisotropy of p
gs. Magn Reson rostate Tissue. M
TI tractograph
e Alfred Hospital, Sydney, Sydney, Nw South Wales, Au
tinct microscopicly restricted diffuy less restricted, and cancer Gleas
mal tissue and dufractional anisot
size that a combiures. This studyd from DTI-tract
tatectomy specimadient set: 2.5 G
0 s/mm2, 6 non-co
hile keeping then algorithm FACT
the fiber tracks u
hown by the dire/mm2. At b ≥ 500
d IS algorithms ppectively). The Re false streamline
ectional color-enectively. To aid v
m DTI data with bre number and loacks accurately rprostate tissue at
Med. 2011 doi: Magn Reson Med
hy
The University of New South Wales, ustralia
c diffusion sion with the D = 0.91 ± 0.17son grade ctal space [2]. tropy (FA) range ination of high-y aims to tography.
men and G/cm/A) using 3Dollinear
sample ductal T. Additional using tensor
ctional color-00 s/mm2, the FA
produced longer RK and IS es into non-
ncoded FA map. visualization, only
b-values in the onger tracks thanreflect stromal t low spatial
d. Accepted 24
D
y
n
1515Proc. Intl. Soc. Mag. Reson. Med. 20 (2012)
