Vapor Sensors Using Olfactory Proteins Coupled to Carbon Nanotubes Mitchell Lerner, Brett Goldsmith,...
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Transcript of Vapor Sensors Using Olfactory Proteins Coupled to Carbon Nanotubes Mitchell Lerner, Brett Goldsmith,...
![Page 1: Vapor Sensors Using Olfactory Proteins Coupled to Carbon Nanotubes Mitchell Lerner, Brett Goldsmith, Joe Mitala, Bohdana Discher, Paul Rhodes, A.T. Charlie.](https://reader038.fdocuments.us/reader038/viewer/2022110207/56649d8c5503460f94a7457e/html5/thumbnails/1.jpg)
Vapor Sensors Using Olfactory Proteins Coupled to Carbon
Nanotubes
Mitchell Lerner, Brett Goldsmith, Joe Mitala, Bohdana Discher, Paul Rhodes, A.T. Charlie Johnson
University of PennsylvaniaAPS March 2010
![Page 2: Vapor Sensors Using Olfactory Proteins Coupled to Carbon Nanotubes Mitchell Lerner, Brett Goldsmith, Joe Mitala, Bohdana Discher, Paul Rhodes, A.T. Charlie.](https://reader038.fdocuments.us/reader038/viewer/2022110207/56649d8c5503460f94a7457e/html5/thumbnails/2.jpg)
Biological Olfaction
• Number of discernable smells is much greater than the number of types of olfactory receptor cells
(350 sensor types detect >10,000 odors for humans)
http://nobelprize.org/
Mammal Types of Olfactory Receptor
Cells
Human 350
Mouse 1000
Rat 1500
Dog 1100
![Page 3: Vapor Sensors Using Olfactory Proteins Coupled to Carbon Nanotubes Mitchell Lerner, Brett Goldsmith, Joe Mitala, Bohdana Discher, Paul Rhodes, A.T. Charlie.](https://reader038.fdocuments.us/reader038/viewer/2022110207/56649d8c5503460f94a7457e/html5/thumbnails/3.jpg)
Experimental Objective
• Integrate biology with nanotechnology
http://nobelprize.org/
![Page 4: Vapor Sensors Using Olfactory Proteins Coupled to Carbon Nanotubes Mitchell Lerner, Brett Goldsmith, Joe Mitala, Bohdana Discher, Paul Rhodes, A.T. Charlie.](https://reader038.fdocuments.us/reader038/viewer/2022110207/56649d8c5503460f94a7457e/html5/thumbnails/4.jpg)
Nanotube Transistors
• Use standard techniques to construct state of the art CNT transistors
![Page 5: Vapor Sensors Using Olfactory Proteins Coupled to Carbon Nanotubes Mitchell Lerner, Brett Goldsmith, Joe Mitala, Bohdana Discher, Paul Rhodes, A.T. Charlie.](https://reader038.fdocuments.us/reader038/viewer/2022110207/56649d8c5503460f94a7457e/html5/thumbnails/5.jpg)
Nanotube Functionalization
Bare NanotubeDiazonium saltEDCNHSNTAOlfactory receptor protein in micelles
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Experimental Design:Attachment
• Confirmation of protein attachment to nanotubes
1mm
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Reproducible Sensing
• curves from 3 experiments showing a reproducible response to eugenol for mOR174-9
• mOR 174-9 responds to eugenol in vivo
blue arrow – inert gas flush
green arrow – eugenol exposure
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Sensor Timing
• experiment showing a response to cyclohexanone for mOR 256-17
• mOR 256-17 responds to cyclohexanone in vivo• Initial response k-1 3s, secondary response is
30s• Recovery k-1 is 30s.
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Concentration Dependence
• experiments showin responses for mOR 203-1• mOR 203-1 responds to 2-heptanone in vivo• fMRI studies show identical responses to 2-
heptanone and n-amyl acetate in mice
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mOR 174-9
mOR 203-1
mOR 256-17
Each of the three proteins gives a different set of reproducible, concentration dependent responses
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Summary• We reproduced many properties of
biological sensing in an electronic device.
• Proteins are maintaining some features found in vivo
• First use of G-protein coupled receptors in electronic sensors
• Potential for application in hazardous material detection and pharmaceutical testing
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Acknowledgements
Evolved Machines Paul Rhodes, Sam Khamis, Ryan Jones
Johnson Group - Penn
A.T.Charlie Johnson, Brett Goldsmith, Mitchell Lerner, Ye Lu
Discher Group - Penn
Bohdana Discher, Joe Mitala
Brand Group - Monell
Joe Brand, Jesusa Josue, Alan Gelperin
Sliger Group - UIUC Stephen Sliger, Tim Baybert
Luetje Group - UMiami
Chuck Luetje
DARPA RealNose Project