Using Real-Time PCR to Determine Blood Type. Lightcycler data.
7
Using Real-Time Using Real-Time PCR to PCR to Determine Determine Blood Type Blood Type
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Transcript of Using Real-Time PCR to Determine Blood Type. Lightcycler data.
Using Real-Time Using Real-Time PCR to Determine PCR to Determine
Blood TypeBlood Type
Example: Blood Type SNPs
Use two assays in a lightcycler:•B assay (which detects the SNP at 796)•O assay (which detects the SNP at 261).
Blood Typing
temp (C)temp (C)
"O"(deletion)
"not-O"(G)
55 65
fluor
esce
nce
""not-B"(C)
"B"(A)
fluor
esce
nce
6255
B Assay O Assay
Group Practice: What’s this person’s blood type?
Type BO
B Assay O assay
""not-B"(C)
"B"(A)
fluor
esce
nce
6255
"O"(deletion)
"not-O"(G)
55 65
fluor
esce
nce
What is the blood type?
Type AO
""not-B"(C)
"B"(A)
fluor
esce
nce
6255
"O"(deletion)
"not-O"(G)
55 65
fluor
esce
nce
What is the blood type?
Type OO
""not-B"(C)
"B"(A)
fluor
esce
nce
6255
"O"(deletion)
"not-O"(G)
55 65
fluor
esce
nce
