Using Plant Tissue Culture to Develop Plants with Acid Soil, Heavy Metal Tolerance (Ahmt),...
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673 USING PLANT TISSUE CULTURE TO DEVELOP PLANTS WITH ACID SOIL, HEAVY METAL TOLERANCE (AHMT), POTENTIALLY USEFUL FOR HARD-ROCK MINE LAND RECLAMATION¹ Sandra King²,Michael King³, Stuart Jennings, and Dennis Neuman. Abstract. Our objective for this project was to determine if we could find, collect, and tissue culture local native plants which grow directly on acid, heavy metal contaminated (AHM) soil. These plants are potentially useful for mine l and reclamation and may possess acid soil, heavy metal tolerance. Explants (small actively growing plant tissue segments) were collected from fifteen plant species growing directly on degraded sites heavily impacted from past hard rock mining activities in Wes tern Montana. These plants, when collected were rooted, surviving and growing in soils characterized by very low soil pH (about 4.0) and high concentrations of various heavy metals. Of the 15 species collected, six were successfully propagated using plant tissue culture, also known as micro- propagation. We concentrated on three of the six species and have attained commercial rates of multiplication and r ooting in-vitro. These three species have been successfully acclimated and grown under commercial greenhouse conditions. Results compar ing our potentially AHMT tis sue cultured plants to non-selected plants of the same species (raised from seed) in test soils under greenhouse conditions will be presented. Future plans for fiel d tests will be described and discussed. Additional key words: Micro-cuttings, in-vitro, site-adapted ____________ ________ 1 Paper was presented at the 2009 National Meeting of the American Society of Mining and Reclamation, Billings, MT, Re vitali z in g the Envir onment: Prove n So lu tion s and I nn ova tive Approaches May 30 – June 5, 2009. R.I. Barnhis el (Ed.) Published by ASMR, 3134 Montavesta Rd., Lexington, KY 40502. ² Sandra King and Michael King, Owners, SMK Plants LLC, Billings, MT 59106, Stuart Jennings and Dennis Neuman, Reclamation Research Group, LLC, Bozeman, MT 59771
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