USER GUIDE Droplet generation pack
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Droplet Generation Pack User guide for droplet generation out of the box If you have any questions, please contact us at contact@elveflow.com 1 Pressure controller OB1 (MK3+) 2 channels - 2 bars 1 PDMS microfluidic chip 10 devices on the same chip 1 Oil and surfactant - 20 mL HFE 7500 + 2% surfactant 2 15 mL reservoirs Microfluidic caps included 1 Tubing 1/32” OD Tubing 30 m 10 1/4”-28 fittings 10 Luer locks fittings 10 1/16” OD FEP Microfluidic Sleeves 1 15m Tygon air tubing 7/32” OD 3/32” ID 1 Pressure source quick connection kit 1 Pneumatic Polyurethane (PU) Flexible Tubing Tubing 2 m with 6 mm OD diameter 2 Flow Sensor No connectors needed 1 ESI Elveflow Software Content of the droplet pack
Transcript of USER GUIDE Droplet generation pack
Droplet Generation PackUser guide for droplet generation out of the box
If you have any questions, please contact us at [email protected]
1 Pressure controller OB1 (MK3+) 2 channels - 2 bars
1 PDMS microfluidic chip 10 devices on the same chip
1 Oil and surfactant - 20 mL HFE 7500 + 2% surfactant
2 15 mL reservoirs Microfluidiccapsincluded
1 Tubing 1/32” OD Tubing 30 m
10 1/4”-28 fittings
10 Luer locks fittings
10 1/16” OD FEP Microfluidic Sleeves
1 15m Tygon air tubing 7/32” OD 3/32” ID
1 Pressure source quick connection kit
1 Pneumatic Polyurethane (PU) Flexible Tubing Tubing 2 m with 6 mm OD diameter
2 Flow Sensor No connectors needed
1 ESI Elveflow Software
Content of the droplet pack
©2018 ELVEFLOW® Microfluidic Innovation Center. All rights reserved. Information is subject to change without notice
www.elveflow.com [email protected] +33(0).184.163.807 2 | 5
The droplet generator pack had been designed to fit most common droplet generation needs of researchers. This fully integrated solution includes all the elements necessary to generate droplets out of the box.
The droplet generator pack is based on our unique piezoelectric flow control technology which allows the user to operate the world’s fastest flow switch, with the smallest variation in flow stability that you can reach in microfluidics.
Design of the PDMS chip
Droplets’ size/pressure diagram
1 2 3oil water outlet
water
oil
oil
oil
water
outlet
outlet
Microfluidic Chip - 10 devices
Choose your input pressure for oil and water to have the droplet size you want
Find table with precise datas in page 5
Droplet diameters in µmThe droplet size is limited to 63 µm on y direction due to the microfluidic channel
diameter. When the value is greater than 63 µm, it is the width of the extended droplet
250 µm
30 µm
50 µm
©208 ELVEFLOW® Microfluidic Innovation Center. All rights reserved. Information is subject to change without notice
www.elveflow.com [email protected] +33(0).184.163.807 3 | 5
Quick start guide
1Step
2Step
Connect your OB1 pressure controller to external pressure supply using pneumatic tubing and to computer using USB cable
For detailed instructions on OB1 pressure controller setup, please read OB1 user guide
Fill your microfluidic tanks with dispersed (water) and continuous phase (oil).
3Step
4Step
Plug microfluidic tanks to the OB1 pressure controller outlet. The Elveflow Reservoirs connection instructions are covered by a specific guide (see Elveflow Microfluidic Reservoirs Assembly Instructions).
If you bought flow sensors, connect them to OB1 for the feedback loop and between microfluidic tanks and chip for flow measurements.
The setup
Collection vialOil
Water
Flow sensor 2
Flow sensor 1Feedback loop
Feedback loop
USB
ELVEFLOW OB1 MK3+
PDMS chip
softwareELVEFLOW
©2018 ELVEFLOW® Microfluidic Innovation Center. All rights reserved. Information is subject to change without notice
www.elveflow.com [email protected] +33(0).184.163.807 4 | 5
Turn on the OB1 by pressing power switch on the front side of the instrument.
8Step
9Step
Add flow sensor: press Add Sensor \ select Flow Sensor \ analog/digital \ max flow rate for the sensor, give the name for the sensor, select to which device and channel sensor is connected and press OK to save the changes.
FordetailsrefertoMicrofluidicFlowSensorUserGuide.
Use the supplied 1/32” OD tubing to connect microfluidic tanks with the chip.
10Step
Set pressures (and other parameters if needed) and start pumping liquids into the chip.Wait until air escapes from the chip and both liquids are flowing.
Change pressure of water channel to start generating monodisperse droplets. Their size, and frequency will depend on the pressure, flow rate and viscosity of the liquids used.
6Step
Launch Elveflow software. The Elveflow Smart Interface’s main features and options are covered by Smart Interface’s guide.
Pleaserefertothoseguidesforadetaileddescription.
7Step
Press Add Instrument \ Choose OB1 \ set as MK3+, set pressure channels if needed, give name for the instrument and press OK to save changes.
YourOB1nowshouldbeinthelistofrecognizeddevices.
5Step
Tips and tricks
Fill the chip first with the continuous phase and make sure that it does not fill the the channel of the dispersed phase by balancing the pressures
Make sure that the output tube from the chip plunges in the liquid of the collection vial to avoid dripping
©208 ELVEFLOW® Microfluidic Innovation Center. All rights reserved. Information is subject to change without notice
www.elveflow.com [email protected] +33(0).184.163.807 5 | 5
Droplets’ size/pressure table
Water p
ress
ure m
bar
Oil pre
ssure
mbar
Droplet
size
x µm
Droplet
size
y µm
Droplet
visu
aliza
tion
Water p
ress
ure m
bar
Oil pre
ssure
mbar
Droplet
size
x µm
Droplet
size
y µm
Droplet
visu
aliza
tion
Water p
ress
ure m
bar
Oil pre
ssure
mbar
Droplet
size
x µm
Droplet
size
y µm
Droplet
visu
aliza
tion
