9:30 RNA by Flow Cytometry: the Power of branchDNA. Speaker: Matthew H. Cato. Field Application Scientist, eBioscience. Measure transcription in heterogeneous populations without sorting. Evaluate transcriptional and translational regulation within the same cell. Identify virally infected cells. Overcome the lack of available anti-body to your novel target. The FlowRNA Assay is a novel in situ hybridization assay built upon dual oligonucleotide probe design, novel chemistry and branched DNA (bDNA) signal amplification, enabling the simultaneous detection of up to three RNA targets together with surface and intracellular Ab staining using flow cytometry. Join us for this technical discussion and learn to see cells differently with Quanti-Gene® FlowRNA.

10:30 The Mouse Transcriptome Array and Transcriptome Analysis Soft-ware (TAC), a fast and simple alternative splicing analysis platform. Speaker: Meg Mintz Kelly, MS, Senior Field Application Scientist, Affymetrix. This presentation will include a live software demonstration of the FREE TAC software.

11:30 Lunch provided for seminar attendees

12:15 Multiplex with Confidence: ProcartaPlex™ Multiplex Immunoassays. Speaker: Matthew H. Cato. Field Application Scientist, eBioscience. Multiplex immunoassays allow simultaneous quantitation of cyto-kine, chemokine and growth factor profiles in complex biologic samples. Several factors impact your ability to validate and translate multi-plexed data throughout your study. Do you have the flexibility to adapt to changing project? Can you correlate your multiplex data to oth-er platforms? Does your quantitation meet international standards? Join us for this technical seminar and learn how to maximize your pre-cious samples and improve your multiplex data quality while reducing time and cost with ProcartPlex multiplex immunoassays.

1:00 QuantiGene™ Plex branchDNA Technology. Unbiased Biology – Deliv-ered! An Additional Application for the Luminex Platform! Speaker: Michael Shure, PhD. Field Application Scientist, Affymetrix. branchDNA technology utilizes XMap® Technology to enable you to quantitate RNA or DNA in a highly multiplexed format without the need for sample purification, cDNA synthesis, or PCR. This is all accom-plished without sacrificing specificity compared to traditional methods. The applications discussed will include target validation, biomarker discovery using FFPE and whole blood samples, pathway analysis, and copy number variation.

2:00 RNA-ISH revolutionized. Speaker: Michael Shure, PhD. Field Application Scientist’ Affymetrix. The in situ analysis of RNA has always been limited by low sensitivity and complicated probe synthesis. The ViewRNA ISH Assay, based on highly specific, branched DNA signal amplification technology, has the sensitivity and robustness for simultaneous in situ detection of up to 4 RNA targets in cells or 2 RNA targets in tissue (frozen or FFPE) with single transcript resolution. This allows for direct, spatial visualization of RNA in complex tissue architectures while retaining tissue mor-phology. This assay can also be completely automated on the Leica Bond RX system. Data from both manual and automated methods will be presented.

For more information : haley_guerrier@affymetrix.com

UPenn/CHOP New Technology Day Date: Friday, September 12th Location: BRB 253 Time: Please see agenda for seminar times RSVP: http://www.ebioscience.com/newtech

Lunch, Morning and afternoon snacks provided with RSVP

Hosted by the Penn Genomic Analysis Core