Understand the concept of microbiota Appreciate the range ...
Transcript of Understand the concept of microbiota Appreciate the range ...
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Understand the concept of microbiota
Appreciate the range of normal microbiota
Understand the importance of surface infections
Describe the range and origins of surface infections on both natural and prosthetic surfaces
Describe the pathogenesis of infections at a surface
Describe the management of infected surfaces
Describe the different types of hypersensitivity reaction
Understand the pathophysiology behind the types of hypersensitivity reaction
Appreciate the clinical significance of hypersensitivity reactions
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is the term used to describe the various bacteria and fungi that are
permanent residents of certain body sites
The viruses and parasites, which are the two other major groups of microorganisms, are
usually not considered members of the normal flora, although they can be present in
asymptomatic individuals.
The members of the normal flora vary in both number and kind from one site to another.
Normal flora organisms inhabit the body surfaces exposed to the environment, such as
the skin, oropharynx, intestinal tract, and vagina. Members of the normal flora differ
in number and kind at various anatomic sites
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the internal organs usually are sterile areas such as the central nervous system, blood, lower bronchi and alveoli, liver, spleen, kidneys, and bladder are free of all but the occasional transient organism.
• Members of the normal flora are low-virulence organisms. In their usual anatomic site, they are nonpathogenic. However, if they leave their usual anatomic site, especially in an immunocompromised individual, they can cause disease.
• Colonization resistance occurs when members of the normal flora occupy receptor sites on the skin and mucosal surfaces, thereby preventing pathogens from binding to those receptors.
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Sterile (Microbe-Free) Anatomical Sites
and Fluids
Sites That Harbor Normal Resident
Microbes
All Internal Tissues and Organs
Heart and circulatory system Bones
Liver Ovaries/testes
Kidneys and bladder Glands (pancreas, salivary)
Lungs Sinuses
Brain and spinal cord Middle and inner ear
Muscles Internal eye
Fluids Within an Organ or Tissue
Blood
Urine in kidneys, ureters, bladder
Cerebrospinal fluid
Saliva prior to entering the oral cavity
Semen prior to entering the urethra
Amniotic fluid surrounding the embryo and fetus
• Skin and its contiguous mucous
membranes
• Upper respiratory tract (oral cavity,
pharynx, nasal mucosa)
• Gastrointestinal tract (mouth, colon,
rectum, anus)
• Outer opening of urethra
• External genitalia
• Vagina
• External ear and canal
• External eye (lids, lash follicles)
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▪ Resident NF – consists of relatively fixed types of microorganisms
regularly found in a given area at a given age; if disturbed, it promptly
reestablishes itself.
▪ Transient NF –consists of nonpathogenic or potentially pathogenic
microorganisms that inhabit the skin or mucous membranes for hours,
days, or weeks; it is derived from the environment, does not produce
disease, and does not establish itself permanently on the surface.
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1.Local Environment (pH, temperature,
O2, H2O, and nutrient levels…).
2. Diet
3. Age
4. Health condition (immune activity)
5. Antibiotics
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▪ Healthy fetus, in utero, is essentially free of MOs
▪ Infant exposed immediately to MOs when passing
through mothers vaginal tract and then to MOs in
environment
▪ Within few hours, oral and nasopharyngeal flora of
neonate established
▪ Within one day, resident flora of lower intestinal tract
established
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Protection from External Invaders
a) Because of the normal flora occupy body's epithelial surfaces, they are able to prevent other bacteria by blocking receptors that
can interfere with colonization by pathogenic bacteria . The ability of members of the normal flora to limit the growth of
pathogens is called colonization resistance .
b) competing for essential nutrients
c) producing anti-bacteria substances e.g : Fatty acids, peroxides , Bacteriocins
May be source of opportunistic infections
e.g : In Patients with impaired defense Mechanisms . For example , antibiotics can reduce the normal colonic flora that
predisposes to pseudomembranos colitis caused by Clostridium difficile .
Nutrition Some of the normal intestinal flora e.g. E. coli & Bacteroids produce Vitamin K in the gut and aid in digestion and
absorption of nutrients.
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Important Members of the Normal Flora
Objective 3
Skin. The predominant member of the normal flora of
the skin is Staphylococcus epidermidis. It is an
important cause of infections of prosthetic heart valves
and prosthetic joints.
Candidia albicans, a yeast also found on the skin, can
enter the bloodstream and cause disseminated infections,
such as endocarditis in intravenous drug users. S. aureus
is also present on the skin, but its main site is in the
nose. It causes abscesses in the skin and in many other
organs.
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Oropharynx. The main members of the
normal flora of the mouth and throat
are the viridans streptococci, such as
S. sanguis and S. mutans . Viridans
streptococci are the most common cause
of subacute endocarditis.
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Examples of bacteria that
inhabit the gastrointestinal
tract.
Gastrointestinal tract. The stomach contains very few organisms
because of the low pH. The colon containsthe largest number of
normal flora and the most diverse species, including both anaerobic and
facultative bacteria. There are both gram-positive and gram-negative rods
and cocci.
The members of the colonic normal flora are an important cause of disease
outside of the colon. The two most important members of the colonic flora
that cause disease are the anaerobe Bacteroides fragilis and the
facultative Escherichia coli. Enterococcus faecalis, a facultative, is
also a very important pathogen.
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Vagina. Lactobacilli are the predominant normal flora organisms in the vagina.They keep the pH of the vagina low, which inhibits the growth of organisms such as C. albicans, an important cause of vaginitis.
Urethra. The outer third of the urethra contains a mixture of bacteria, primarily S. epidermidis . The female urethra can become colonized with fecal flora such as E. coli , which predisposes to urinary tract infections.
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Associations between microbes and
humans. Effects of contact with
microbes can progress in a variety of
directions, ranging from no effect to
colonization, and from infection to
disease and immunity. The example
shown here follows the possible events in
the case of contact with a pathogen such
as Streptococcus pneumoniae (the
pneumococcus). This bacterium can be
harbored harmlessly in the upper
respiratory tract, but it may also invade
and infect the ear, cranium, and
respiratory tract.
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•The two main ways in which an individual will encounter a pathogen are:
where the normal microbiota can multiply and develop due to a disruption of normal defences
result when infecting bacteria have been acquired from an external source and is not part of the natural microbiota
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Pili (fimbriae): Are hairlike filaments that extend from the cell surface , they are shorter and straighter than flagella and composed of subunits of aprotein , pilin. They mediate attachment of bacteria to specific receptors witch is necessary step in initiation of infection . For example, Escherichia coli
Glycocalyx (Slime layer): Is a polysaccharide coating that is secreted by many bacteria . It cover surface like afilm and allows the bacteria to adhere firmly to various surface such as skin, heart, valves, and catheters .Streptococcus mutans adhere to the surface of teeth lead to formation of plaque the precursor of dental caries .
Capsule : Is a gelatinous layer covering the entire bacterium . It is composed of polysaccharide except inthe anthrax bacillus which have capsule of polymerized D-glutamic acid . Poly- saccharide capsuleprevent phagocyte from adhering to bacteria.
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M protein: Extend from an anchor in the cell membrane , through the cell wall and then thecapsule and exposed on the surface of the bacterium. M protein are antiphagocytic and theyform a coat that interferes with complement binding. Streptococcus pyogenes attach to thepharyngeal mucosa via action M protein and cause pharyngitis.
Protein A : Is the major protein in cell wall of Staphylococcus aureus, it is an importantvirulence factor because it bind to the Fc portion of IgG at the complement binding sit thereby
preventing the activation of complement.
Surface proteins
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Collagenase and hyaluronidas: Which degrade collagen and hyaluronic acid thereby
allow bacteria to spread through subcutaneous tissue they are especially important in
cellulitis casued by Streptococcus pyogenes .
Enzymes secreted by invasive bacteria
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Coagulase: Which is produced by staphylococcus aureus and accelerates theformation of a fibrin clot from its precursor fibrinogen (this clot may protectthe bacteria from phagocytosis)by coating the organisms with a layer of fibrin.
Coagulase test: Coagulase is an enzyme that causes a clot to form when bacteria areincubated with plasma .The test is used to differentiate Staphylococcus aureus (coagulasepositive) from coagulase-negative staphylococci S. epidermidis and S. saprophyticus
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– Invasion
• e.g. Strep pyogenes pharyngitis
– Migration
• e.g. Escherichia coli urinary tract infection
– Innoculation
• e.g. Coagulase negative staphylococcus prosthetic joint
infection
– Haematogenous
• e.g. viridans Strep endocarditis
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What is a surface? Objective 4Interface between a solid and either a liquid or gas.
Patient
Pathogen
Infection Management Outcome
Mechanism of infection
epithelium
hair
nails
conjunctival
gastrointestinal
respiratory
genitourinary
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• External • Internal
– Cellulitis – Endovascular
– Pharyngitis – Endocarditis
– Conjunctivitis – Vasculitis
– Gastroenteritis – Septic arthritis
– Urinary tract infection – Osteomyelitis
– Pneumonia – Empyema
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Intravascular lines
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Peritoneal dialysis
catheters
Prosthetic joints
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Pacing wires
Cardiac valves
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Endovascular grafts
Ventriculo-peritoneal shunts
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Prosthetic valve endocarditis
HACEK group: Haemophilus, Aggregatibacter, Cardiobacterium, Eiknenella, Kingella
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Prosthetic joint infections
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Cardiac pacing wire endocarditis
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Processes in the pathogenesis of infection at surfaces
Objective 5
• Adherence to host cells or prosthetic surface
• Biofilm formation
• Invasion and multiplication
• Host response
– Pyogenic (neutrophils -> pus)
– Granulomatous (fibroblasts, lymphocytes,
macrophages -> nodular inflammatory
lesions)
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What is Biofilm
Biofilms are multicellular aggregates of bacteria and yeast
that congregate on surfaces.
Biofilm formation is a nearly universal bacterial trait, and
biofilms are found on almost all natural and artificial
surfaces
Biofilms are formed to protect the bacteria from host
defenses, antibiotics, and from harsh environmental
conditions.
Some examples of biofilms are the plaque that forms on
teeth and the slime that forms on surfaces in watery areas
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A process that enables bacteria to
communicate using secreted signaling
molecules called auto inducers
This process enables a population of
bacteria to regulate gene expression
collectively and therefore, control behavior
on a community-wide scale.
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Quorum Sensing helps in
Bacteria use Quorum sensing to
mastermind behaviors or (Controls)
– Sporulation
– Biofilm formation
– Virulence factor secretion
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Three principles steps
1) production of small biochemical signal molecules by the bacterial cell.
2) release of the signal molecules, either actively or passively, into the surrounding
environment
3) recognition of the signal molecules by specific receptors once they exceed a
threshold concentration, leading to Gene expression ->co-operative behaviours and
more production.
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Natural surface
• Maintain surface integrity
• Prevent bacterial surface colonisation
• Remove colonising bacteria
Prosthetic surfaces
• Prevent contamination
• Inhibit surface colonisation
• Remove colonising bacteria
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Question
A-Pseudomembrane colitis is caused by the strains of which of the following microorganism?
1-Streptococcus mutant
2-Clostridium difficile
3-Streptococcus saprophyticus
4- Clostridium botulinum
B- Where does the Streptococcus mutants adhere to the body site
1- intestinal tract
2-tongue
3-teeth
4-hairs