U1L6 - Lab Equipment...Lab Equipment In order to complete lab requirement for this course you will...
Transcript of U1L6 - Lab Equipment...Lab Equipment In order to complete lab requirement for this course you will...
Lab Equipment
In order to complete lab requirement for this course you will learn proper use of a variety of measuring devices (some of which you are already familiar with)
Some of equipment that you may use includes:RulerElectronic balance Graduated cylinderPipetteThermometerpH paper and pH meterColorimeterDissolved oxygen probe
Ruler
There is uncertainty at both ends of
ruler
Electronic Balance
00.0 g.
The uncertainty is
known(± 0.1 g)
✦ plug in balance✦ turn on balance and wait for it to show zero✦ place weigh paper (container) on balance and press zero button ✦ place your sample on weigh paper, wait for reading to stabilize and
record value
Graduated Cylinder
There is only uncertainty at
meniscus
✦ take measurement at eye level✦ measure to bottom of meniscus
Pipette
When rinsing:‣ draw a little air into the pipette
before rinsing with solution‣ wipe the tip of the pipette after
each rinse to avoid contamination‣ rinse the pipette 3 times
‣ choose the appropriate pipette (0.1 ml, 1 ml, 2 ml, 5 ml, 10 ml)
When transferring the liquid:‣ fill the pipette completely‣ lower the meniscus to the line‣ drain the pipette keeping it
upright and in contact with the receiving container
‣ some solution will remain in the pipette (do not expel it)
The uncertainty is
printed on pipette
✦ stir solution with a stirring rod (not thermometer)✦ wait for temperature reading to stabilize (stop changing)✦ record temperature value (estimate value between gradations)
Thermometer
There is only uncertainty at
one end of thermometer
°C°F
✦ suspend thermometer in solution with use of a retort stand (thermometer should not touch container)
pH paper & pH meterpH paper & pH meter
pH paper & pH meter
The uncertainty is
known(± 0.1)
✦ calibrate probe with solutions of known pH✦ measure pH of sample solution
A colorimeter measures absorbance of particular wavelengths of light by a solutionIt can be used to determine relative concentration of a given solute, since concentration of a solute is proportional to absorbance
Colorimeter
The uncertainty is
known(± 0.15)
Dissolved Oxygen Probe
Dissolved oxygen probe can be used to measure oxygen production of photosynthesizing aquatic plant✦ calibrate probe✦ set up photosynthesis kit✦ turn on light and begin logging data
The uncertainty is
known(± 10% of full
scale)