Two States of the Array

B. Ding & N.C. Seeman, Science 314, 1583-1585 (2006).

Transitions in the Array

B. Ding & N.C. Seeman, Science 314, 1583-1585 (2006)..

Transitions in the Array (Zoom)

B. Ding & N.C. Seeman, Science 314, 1583-1585 (2006).

Programming an Array for Assembly

A. Carbone & N.C. Seeman, Proc. Nat. Acad. Sci. (USA) 99 12577-12582 (2002).

A Ribosome-Like Device:

A DNA Nanomechanical Translation Machine

Shiping Liao

A Drawing of the Device

Liao, S. & Seeman, N.C. (2004), Science 306, 2072-2074.

Movable Sticky Ends

Movable Sticky Ends

Fixed Sticky Ends

Principles of Operation

I1 2 74

3 5PX1 JX22

6

II

III

V

IV

1 2 7

3 65

4

PX1 JX22

I II

III

IV

V

1 3DX3

1 2DX2

4 6DX4

4 7DX5

5 7DX7

5 6DX6

DX2 DX5

Liao, S. & Seeman, N.C. (2004), Science 306, 2072-2074.

Possible Products

Liao, S. & Seeman, N.C. (2004), Science 306, 2072-2074.

Relationship toTraditional Components

2 4 60 1I II

III

IV

VA B

3 75PX1 PX2

Message Codon Equivalent Message Codon Equivalent

Amino Acid EquivalenttRNA Equivalent

Liao, S. & Seeman, N.C. (2004), Science 306, 2072-2074.

DX Molecules Correspond to Aminoacyl tRNA Molecules

Anticodon Equivalent

Sequence

NNN------------------------------------NNN

1. MS beads

2. Release (Cleave DS2)

3. Ligate

4. Denature

IVIII

VI II

A B

0 1 2 74

3 65PX1 JX22

IV

VI I I

PX1 JX22A B

0 1 2 74

3 65I I I

FailedAssemblies

B

1. MS beads2. Release (Cleave DS1)

3. Add DS2

Purification, Ligation & Analysis

Liao, S. & Seeman, N.C. (2004), Science 306, 2072-2074.

Sequencing Result

*

All Combinations WorkLiao, S. & Seeman, N.C. (2004), Science 306, 2072-2074.

Polymer Unit

Visualized Application

DX Molecule

Organized Polymers

DNA Walking Biped

Bill Sherman

Schematic of the Device and Sidewalk

Sherman, W.B. & Seeman, N.C. (2004), NanoLett. 4, 1203-1207.