Tubular Organs

Tracheal Development

DT

LT

DB

Dysfusion (Dys) is a bHLH-PAS Protein

bHLH PAS-1 PAS-2

Dysfusion Is Expressed In Fusion Cells

DT

DB

LT

Btl-GAL4, UAS-lacZ Dys

dys Mutants

dys

3

dys

2

bHLH PAS-1 PAS-2

Mab 2A12

WT dys

How dys expression is regulated?

How Dys:Tgo regulates down-stream target genes?

Dysfusion is Expressed in Mutiple Cell Types

Identify dys Enhancer

Identify dys Fusion Cell Enhancer

How dys expression is regulated?

•Is dys auto-regulated by Dys:Tgo?

N283-GFP,dys/Tm3, twi-gal4, UAS-GFPC1.7-GFP, dys/TM3, twi-gal4, UAS-GFP

•Is dys regulated by esg?esg/cyo, twi-gal4, UAS-GFP; N283esg/cyo, twi-gal4, UAS-GFP; C1.7

•Identify other transcription factors binding sites in dys enhancer.

Identify dys fusion cells enhancer in C1.7 and G1.7

Ongoing……

Summary of dys Function

N

TC GTG

members only (Protein trafficking)

Trachealess (Migration)

CG13196, E-cadherin (cell adhesion)

CG15252 (unknown function)

TgoDys

TgoDys

TgoDys

Identified dys Targets

Two TCGTG Sites are Required to Activates CG13196

Two TCGTG Sites of CG13196 are Conserved

Two TCGTG Sites are Required to Activates CG15252

Two TCGTG Sites of CG15252 are Conserved

Potential Co-activator of Dys:Tgo is Required to Turn on Gene Expression in Additional Fusion Cells

Ongoing..