INTRODUCTION:-

In general, many advances were made in medicine and development

of pharmaceutical industry, but the use of herbal medicine has not been

reduced which is true for the advance countries as well as in under

developed countries. It is some what paradox, therefore, that at a time when

there is unprecedented number of the therapeutic drugs available for the

treatment of disease, that herbal remedies continue to be demanded by the

general public. In fact, this demand is increasing each effective herbal

remedies. WHO has done some work on it and they made some criteria in

respect of evaluation / determination of adverse effect and features of

herbal remedies and that are general information like scientific name,

synonym, vernacular names, geographical distribution, description, plant

part, organoleptic properties, microscopic characteristics, powered plant

material, general identification test, purity test, pesticide residues, heavy

metals, radioactive residue, chemical assay, major chemical constituents

medicinal use supported by chemical data and its pharmacology.

Major problem is the nature of herbal ingredients, which are

complex mixtures of constituents and that varies with environment and

genetic factors. Furthermore, the constituents responsible for the claimed

therapeutic effects are frequently unknown or partly explained. Control of

herbal ingredient in starting material and finished product is also problem

and needs extensive studies and researches. The extracts of Tridax

procumbens have been reported to have various pharmacological effects,

like antimicrobial activity, anti-inflammatory activity, Antidysentery,

Antidiarrhoeal, Hypoglycemic effect, Antibacterial, Antiseptic, Insecticidal

properties and to prevent the falling of hair.

Biological source: -

It consist of dried wholes plants of tridax procumbens belonging to family Asteraceae.

Leaves:-

Bronchial catarrh, dysentery, diarrhea and hair restorative.

Leaf juice:-

Antiseptic, insecticidal , parasiticidal properties and is used externally to stop bleeding from cuts.

Scientific classification

Kingdom: Plantae(unranked): Angiosperms(unranked): Eudicots(unranked): AsteridsOrder: AsteralesFamily: AsteraceaeTribe: HeliantheaeGenus: TridaxSpecies: T. procumbensBinomial name: Tridax procumbens L

PLANT INTRODUCTIONS:-

Photographs

Synonym: - Ekapushpi

Botanical Name: - Tridax procumbens Linn

Family: - Asteraceae

Local name: - Dhaman grass

Diff. Language Name: - Dagadi pala in Marathi, ghamra in Hindi, coat buttons in English, Jayanti Veda in Sanskrit

Useful Part Used: - Whole plants

PLANT DESCRIPTION: -

A spreading annual herb grows up to 20 cm in height. Leaves simple, opposite,

serrate or dentate, acute, fleshy and pubescent. Flowers in head, long stalked and

whitish. Seeds are numerous, small with tuft of silky hairs on one side for wind

dispersal.

LITERATURE SURVEY:-

Upon literature survey it is found that various extract of different part of this

plant are used in the indigenous system of medicine as antimicrobial activity, anti-

inflammatory activity, Antidysentery, Antidiarrhoeal, Hypoglycemic effect,

Antibacterial, Antiseptic, Insecticidal properties and to prevent the falling of hair. The

leaves are reportedly used to treat bronchial catarrh, dysentery, diarrhoea and as a hair

restorative. A paste prepared from the whole plant is taken orally to relieve diarrhoea.

A fine paste of the leaves is applied externally to reduce swelling of hemorrhoids. The

leaf powder mixed with that of Cicer arietium (fabaceae papilionoideae) in a 2:1 ratio is

taken orally to treat diabetes. Leaf juice possess antiseptic, insecticidal and parasiticidal

properties and is used externally to stop bleeding from cuts. The whole plant and seed

being used to treat a variety of ailments. A large number of Alkaloids, Tannin, Saponin

and Flavonoids are possessing noteworthy biological and pharmacological properties.

They have been isolated from leaves, flowers, stem and seeds.

MEDICINAL PROPERTIES

DIFFERENT ACTIONS

WHOLE PLANT PASTE

LEAVES PASTE

LEAF POWDER

LEAF JUICE

Antiseptic & to stop bleeding

Diabetic

To reduce swelling of haemorrhoids

Diarrhoea

Distributions: - This plant grows in tropical and subtropical regions of all over India

and it is found in gardens as ornamentals. Fresh leaves of Tridax Procumbens collected

from the botanical garden and villages of Pune district, Maharashtra (India) during the

month of June and July and were identified by the botany department of C. T. Bora

College of Science, Shirur.

PRELIMINARY STUDIES:-

Pitta

Wound

Ulcers & Fistula

Hemorrhoids

Inflammation

Organoleptic character

Determination of Ash Value % (w/w)

Determination of Extractive Values

Microbial Test

Microscopical studies

DOSAGE:-

Dose %Inhibition Action

400mg/kg 52.50 Anti-inflammatory

250mg/kg 68.26 Anti-diabetics

500mg/kg 71.03 Anti-diabetics

EXTRACTION PROCESS:-

Material and method:-

a) Infusion

b) Maceration

c) Percolation

T. procumbens was extracted by simple maceration process. 300g of fresh

leaves were ground with the help of mortar and pestle. Juice obtained was filtered

through muslin cloth. The filtrate was evaporated by rotary evaporator (cryochiller).

The extraction was filtrated and dried by rotary evaporator.

METHOD OF PREPARATIONS :-

Uses described in folk medicine, not supported by experimental or clinical data:

Bronchial catarrh, dysentery, diarrhoea, hair restorative, insecticidal and

parasiticidal properties and it is used externally to stop bleeding from cuts.

RESULT AND DISCUSSION:-

(A) Organoleptic character:

Part: - Leaves

Arrangement: - Opposite

Size: - 3-7 cm long, 1- 4 cm wide

Shape: - Lanceolate to ovate

Colour: - Green

Odour: - Characteristic

Taste: - Acrid

Appearance: - Rough & Scabrous

Margin: - Irregularly toothed

Apex: - Acute

Base : - wedge shaped

Texture: - Short

(B) Determination of Ash Value % (w/w)

1. Total ash: - 11.88

2. Acid insoluble ash: - 3.05

3. Water soluble ash: - 2.41

4. Sulphated ash: - 20.11

5. Loss on drying: - 13.00

(C) Determination of Extractive Values

Solvent Value % (w/w)

1. Haxen : - O8.90

2. Ethanol: - 07.17

3. Water: - 28.16

(D) Microbial Study:

(1)Material is free from other than original plant source.

(2) Free from insects, moulds or other animal contamination.

(3) ----------------------------------------------------------------------------------

(E) Quantitative Microscopical studies:-

Leaf constant Upper

epidermis(/mm2)

Lower

epidermis(/mm2)

Vein islet number 32.33 20.66

Vein termination number 32.66 19.66

Stomatal index 39.12 36.31

Trachoma number 9.66 21.33

(F) Major chemical constituents:

(1) ß-sitosterol-3-0-ß-D-xylopyranoside

(2) Quercetin,

(3) Dexamethasone

(4) Luteolin

(5) Glucolutin.

(G) General identity test:-

Macroscopic and microscopic examination, chemical tests, and thin-layer

chromatography for the presence of the presence of diterpens lactones.

(H) General identification and chemical test:-

1.Saturated picric acid + Plant extract

Yellowish- green

2.Nitric acid + Plant extract

Radish- brown

3.Hydrochloric acid+ Plant extract

Green

4.Sulphuric acid (80%)+Plant extract

Black

5.Glacial acetic acid+ Plant extract

Dark green

6.Iodine solution (N/20) + Plant extract

Dark green

7.Ferric solution (5% W/V aq. Solution) + Plant extract

Yellowish-brown

8.Powder as such+ Plant extract

Green

PHYTOCHEMICAL SCREENING: -

In 2008, Dhanabalan, Doss, Balachandar, Vaidheki and Kalamani reported the

presence of an alkaloid, tannins, saponin, steroid and flavonoid. The Leaf of T.P.

mainly contain crude proteins (26%), crude fiber (17%), soluble carbohydrates (39%)

and calcium oxide (5%) In (Chadha 1976).

INDUSTRIAL APPROACH:-

FUTURE PROPOSED WORK:-

(1) Potential quality & Standardization by means of HPTLC:-

It is a preliminary techniques (analysis of one or group of samples can be

detected) and also combined action of several variables to check the potency of

chemical constituents by using various mobile phases.

(2) Structure activity relationship study:-

In a Structure activity relationship (SAR), a specific biological activity of

structurally related compounds is evaluated. The most active compound with the least

side effects will be selected as a lead structure for further development into a drug

candidate.

Structure activity analysis of the tested compounds will determine the

molecular structures responsible for the interactions with receptors on target organs.

Eventually, a series of compounds related to the most active structure will be

synthesized to improve the desired activity and to reduce or eliminate the unwanted

biological activity. The lead compound in the class will be selected for total synthesis.

(3) Different Pharmacological Activity (In-vivo Analysis):-

Like Pitta, Coagulant, Ulcer, Bronchial catarrh, dysentery, diarrhoea, hair

restorative.

CONCLUSIONS:-

As per the objective of our research, the phytochemical and pharmacological

studies of the plant, Tridax procumbens L. are done which can be of use to future

researchers. Phytochemical study revealed the presence of secondary metabolites such

as Alkaloids, saponin, steroids, tannins, flavonoids. Tridax procumbens L.

(Asteraceae) is known for several potential therapeutic activities like antiviral,

antibiotic efficacies, wound healing activity, insecticidal and anti-inflammatory

activity. The present paper highlights the exomorphology and histomorphology of leaf,

petiole, internode, root, physicochemical evaluation and preliminary phytochemical

study of the whole plants. These observations will enable to standardize the botanical

identity of the drug in crude form.

REFERENCE:- 1. The Wealth of India: Raw materials, VoI.10 (Publication and

Information Directorate, CSIR, New Delhi) 1988, 87-88.

2. Asima Chatterjee, the Treatise of Indian medicinal plants. (Satyesh Chandra

Prakashan, CSIR, New Delhi) 2001, Vol. 5, 180.

3. Parrota J. A. Healing plants of peninsular India. (CAB International publishing)

2001, 157-158.

4. Vyas. P. Suresh, Tiwari Umesh, Rastogi Bhawna, Singh Paramjit,

Immunomodulatory effects of aqueous extract of Tridax procumbens in experimental

animals, (Journal of Ethnopharmacology 92), 2004, 113-119.

5. Devaki Thiruvengadam, Ravikumar Vilwanathan, Shivashangari S.K,

Hepatoprotective activity of Tridax procumbens against

D-galactosamine/lipopolysaccharide-induced hepatitis.