Toward the industrialization of Okinawa biological resources · 2019. 2. 15. · ・ Measurement of...

Toward the industrialization of Okinawa biological resources

Name Shinya Ikematsu E-mail [email protected]

Status Vice-President ( Research affairs ), Professor

Affiliations

JBS (The Japanese Biochemical Society), MBSJ (The Molecular Biology Society of Japan), JCA (The Japanese Cancer Association), JSPHO (The Japanese Society of Pediatric Hematology／Oncology), JSEE (Japanese Society for Engineering Education), JSOG(The Japan Society of Obstetrics and Gynecology), JSBBA(Japan Society for Bioscience, Biotechnorogy, and Agrochemistry) and JSBi (Japanese Society for Bioinformatics)

Keywords Biological resources,・Regenerative medicine,・lactic acid bacteria,・Development of new

technology,・Functional materials,・Pharmaceuticals,・Cosmetics,・In-vitro diagnostics

Technical Support Skills

・ General analysis- material analysis and gene analysis (including Bioinformatics) ・ Process management, mainly GMP ・ Development of human resources (Bioinformatics technician, Clinical embryologist )

Available Facilities and Equipment

DNA sequencer, CEQ8800 (Beckman Coulter) NGS, MiSeq (illumine） HPLC & UPLC/MS (Waters) Cell sorter Fluorescence microscope, Axio Imager Z2 (Carl Zeiss Microscopy) Safety cabinet

Realtime PCR, 7500 (Applied Biosystems) FPLC AKTA explorer (GE healthcare)

Department of Bioresources Engineering

National Institute of Technology, Okinawa College

Research

contents：

mailto:[email protected]

Development and application of cell-free protein synthesis system

Name ITO Masaaki E-mail [email protected]

Status Professor

Affiliations Japan Society for Bioscience, Biotechnology, and Agrochemistry. The Japanese Biochemical Society

Keywords Cell-free protein synthesis system, insect, silkworm, polyphenol oxidase

Technical

Support Skills

・ Recombinant DNA experiment

・ Screening, purification and analysis of enzymes

Research Contents Development and application of insect cell-free protein synthesis system

Insect cell-free protein synthesis system


Spectrophotometer, DU800（Beckman Couｌter） Microplate Reader, infinite M200（TECAN）

Spectrophotometer, DU800（Beckman Couｌter） Microplate Reader, sunrise rainbow thermo（TECAN）

Chromatography system, AKTA avant（GE Healthcare） Microchip Electrophoresis System, MultiNA（SHIMADZU）

Chromatography system, AKTA prime plus（GE Healthcare）

Cell disruptor, MULTI-BEADS SHOCKER（YASUI KIKAI）

KOSEN National Institute of Technology

Department of Bioresuorces Engineering


Name Junsei Taira E-mail [email protected]

Status Professor

Affiliations ・The Pharmaceutical Society of Japan ・American Chemical Society

Keywords ・Oxidative stress, Antioxidant, Cosmetics, Functional food

Technical

Support Skills

・Evaluation of functional materials in bioresources

・Isolation of functional compounds

Research Contents

My research target is to find the active compounds as potential useful functional food, pharmaceutical

cosmetics and drugs in bioresources (plants1-4) and marine natural products5-6) and also make the aroma

profiles in beverages 7). The research flow indicated in the under figure and general evaluation is as follows.

Polyphenol content, antioxidant activity (ORAC method, ESR study), anti-inflammatory effect, antitumor

activity, apoptoic activity and inhibition of melanogenesis.

Recent research publications are as follows.

1) Oxidants and Antioxidants in Medical Science. 2, 21-28, 2013

2) Journal of Food Composition and Analysis. 29, 117-125, 2013

3) Journal of Agricultural and Food Chemistry. 62, 167−172, 2014

4) Food Chemistry. 166, 531-536, 2015

5）Marne Drugs. 10, 2741-2748, 2012

6) Tetrahedron Letters. 55, 1421-1423, 2014

7) Food Science and Technology Research. 18, 177 -181, 2012


Large scale headspace GC/MS Agilent Technologies

LC/MS Agilent Technologies

LC for analysis and preparative isolation Agilent Technologies

DNA analyzer Agilent Technologies

Department Bioresources Technology


Find Assay

Analysis

Natural products


Development of Possible Applications for Local Food Resources

Name TANAKA Hiroshi E-mail [email protected]

Status Professor

Affiliations Japanese Society for Bioscience, Biotechnology, and Agrochemistry

The Japanese Society for Food Science and Technology

Keywords Local resources, Lactic acid bacteria, Food processing, Marketing

Technical

Support Skills

・ Preparation of foods and/or ingredients

・ Nutrient analysis and functionality evaluation

・ Functionality of lactic acid bacteria

・ Development of marketing plan for new products

Research Contents Converting local natural resources to food ingredients with added values

1. Development of food processing methods utilized by original microbes

Development of food processing methods by lactic acid bacteria isolated in Okinawa

Isolation of local marine lactic acid bacteria with unique properties

Identification of isolated lactic acid bacteria by sequencing of 16S rRNA

Screening of antimicrobial activity of isolated local lactic acid bacteria

Characterization of isolated lactic acid bacteria for their fermentation properties

2. Utilization of local botanical resources

Construction of an in vitro assay method for induction activity of detoxification enzymes

Screening of extracts of local vegetables and fruits for the induction activity

Purification and identification of active substance(s) of the induction activity

Characterization of Ryukyu-Ai (Strobilanthes cusia) extracts for antimicrobial and antiviral activities

3. Utilization of local animal resources

Evaluation of goat milk for its food processing characteristics

Development of original goat cheese with new rheological properties

Analysis of rheological properties of goat milk

Development of goat milk based alcoholic beverages with fermentation

Analysis of aroma constituents by GCMS

4. Marketing strategy

Development of marketing strategy for utilization of local natural resources

Consultation of marketing method for new product development


Lactic acid bacteria culture system

UPLC (Waters)

Cell culture system

Cooking laboratory

Marketing mix

Department of Biorsources and Engineering,


Bioresources and Photochemistry

Name HAMADA Taisuke E-mail [email protected]

Status Professor

Affiliations

The Chemical Society of Japan, Japan Society of Coordination

Chemistry, The Electrochemical Society of Japan, Japanese Society

of Engineering Education

Keywords Photochemistry, Physical chemistry, Coordination chemistry

Technical

Support Skills

・ Measurement of photochemical property

・ Supercritical fluid extraction

・ Photo energy conversion

Research Contents

[Dye sensitized solar cell]

Solar energy have attracted much attentions because it is clean, and it is called green energy. Solar cell

is device to convert photo-energy to electric energy. We have been studied a solar cell, which is called the

dye sensitized solar cell (DSSC).

We have made DSSCs and studied the effect of the dye on the efficiency under irradiation of sunlight (AM

1.5G) from the solar simulator (Fig. 1). In this study, we have evaluated the photochemical and

photoelectrical properties of the dye. Therefore, we are able to measure and to evaluate the photochemical

property of various compounds in order to clarify a photochemical ability.

[Super critical fluid extraction]

We have been trying to obtain the useful compound such as poly-methoxy flavonoid (PMF) by means of

super critical CO2 extraction method. The merit of this method is that the temperature for the extraction

is relatively low and CO2 dose not remain in an extract.


Solar simulator (WACOM)

Supercritical CO2 extraction apparatus (AKICO)


Okinawa National College of Technology


Department of Bioresources EngineeringNational Institute of Technology, Okinawa College

Research of the Okinawan traditional distilled liquor

“AWAMORI”

Name Yasutomo Yamaki E-mail [email protected]

Status Associate Professor

Affiliations

Japan Society for Bioscience, Biotechnology, and Agrochemistry

The society for Biotechnology, Japan

The society of Tropical Resources Technology

Keywords Awamori, fermentation, microoganizms, Black koji-mold,

Technical

Support Skills

・ The whole about the AWAMORI. / ・Aromatic component analysis.

・ Alchoholic beverages development of products. / ・Fermented food development.

Research Contents Research of the microbe which has an influence on the quality of Awamori.

Koji

MoromiSampling

Isolation ofmicrooganisms

Experimental brewing

Identification ofmicrooganisms

Preservation ofmicrooganisms

Analysis of qualityof liquor

Elucidation ofthe function

Develotment ofnew Awamori

Awamori

Establishment ofbrewing method


名称・型番（メーカー）

Gas Chromatograph Mass Spectrometer （GC-MS : Shimadzu） Headspace automatic sampler system （Perkin Elmer）

High Performance Liquid Chromatography （HPLC : Shimadzu） Biophotorecorder （ADVANTEC）

Differential scanning calorimeter (DSC : Shimadzu) Thermal Analysis (TA : Shimadzu)

Jar fermenter ( Takasugi) Clean bench (AIRTEC)

Autoclave (Tomy) Thermo-hygrostat (EYELA)

Development of functional food materials from subtropical bioresources

Name Takahiro MITSUE E-mail [email protected]

Status Professor

Affiliations

Japan society for food science and technology., Japan society for Biotechnology., Japan society for Bioscience, Biotechnology, and Agrochemistry.

Keywords Microorganisms, Food product., Quality control

Technical

Support Skills

・ Fermentaion of every materials

・ Establishment of manufacturing process

・ Quality control (ISO, HACCP)

Research Contents

Biochemical scientists are expected to solve the problems of life

science, use of energy and bioresources, and surroundings.

Nowadays, it is possible to produce new biomaterials with excellent

functions.

Also, we are trying to develop the functional food or medical

materials from subtropical bioresources in Okinawa.


HPLC (Shimazu)

Jarfermentor (Takasugi)

Microscope (SEM,Optical)

Capillary for electrophoresis (Agilent)

Color difference meter (Konica)



National Institute of Technology, Okinawa

College


Capillary Sequencer: CEQ8800 (Beckman Coulter) Next Generation Sequencer MiSeq (illumine)

Search for biodiversity and function of marine organisms and application to conservation

Name Naoko Isomura E-mail [email protected]

Status Assistant Professor

Affiliations The Zoological Society of Japan, The Ecological Society of Japan, Japanese Coral Reef Society

Keywords Corals, Reproduction, Reproductive ecology, Genetic differentiation, Speciation

Technical

Support Skills

・ Genotyping using sequences and microsatellite marker

・ Collecting gametes, cross experiments and rearing juveniles of marine organisms

・ Collecting marine organisms by SCUBA

・ Microbial community analysis by NGS (not main theme)

Research Contents

In my study, my purposes are to search biodiversity of marine organisms, especially scleractinian corals,

and to apply for conservation.

My purpose is to estimate the genetic diversity and its function of marine organisms in Okinawa. First, I

will try to clear morphological and/or diversity of species and population. And then, after I search and

clarify the endocrine system of organisms by physiological and molecular analyses, I will evaluate the

relationship between these system and ecological characteristics of organisms. Moreover, I will investigate

conservation method for target organisms using my results.




Cloning of the gene for plant stress responsiveness

Name SANMIYA Kazutsuka E-mail [email protected]

Status Associate professor

Affiliations The Japanese Society of Plant Physiologists The Molecular Biology Society of Japan

Keywords plant stress responsive gene, isoprenoid, heat shock protein, saponin

Technical

Support Skills

・ Gene cloning

・ Gene expression analysis

・ Transgenic plant

Research Contents FPPS gene, heat shock protein gene, UGT gene, Rice yield

● Cloning of the gene for FPPS

For the first time, the gene for chloroplast FPPS was cloned. FPPS is the key enzyme for isoprenoid biosynthesis.

Isoprenoids play roles in plant stress response such as protection against sunlight.

● Heat tolerant transgenic tobacco The gene for heat shock protein was used for the transformation (Fig).

● Cloning of the gene for UGT

For the first time, the gene for saponin biosynthesis was cloned. Saponin protects the Brassicaceae crops, cabbage, radish, broccoli against pests.

● Rice yield increased to 124% by glass silica


Electrophoresis apparatus Mupid (ADVANCE)

Gene expression analyser AE6933 (ATTO)

Thermal cycler TP450 (TaKaRa)



wild type

tobacco

heat tolerant

transgenic tobacco


Research Contents

Food processing, non-thermal sterilization and high efficiency extraction using the shock wave

Name Takemoto, Ayumi E-mail [email protected]


Affiliations

The Japan Society of High Pressure Science and Technology

The Japan Society of Cookery Science

The Materials Research Society of Japan

Keywords Shockwave processing, Flouring, Extraction, Non-thermal sterilization

Technical

Support Skills

・ Shock wave processing to biosystem

・ Non-thermal milling, Efficient extraction


Rheometer , RE2-33005C (YAMADEN co.,ltd)


National Institute of Technology, Okinawa College KOSEN National Institute of Technology

mailto:[email protected]

Application of Okinawan microorganisms

Name Toshiaki TANABE E-mail [email protected]


Affiliations JSBBA, SBJ, JSMSB, JSCC, MSJ, and BSO

Keywords Chitin, Chitosan, White-rot fungi,

Technical

Support Skills

・ Component analysis of biomass

・ Screening of bacteria or enzymes for bioremediation or bioconversion

Research Contents

Construction of Okinawan Soil library

The soil samples were collected from approximately 2,600 places including each remote island in

Okinawa, and were constructed to the Okinawan soil library. Various kinds of microbes are isolating from

this library. They have various bioactive substances and enzymes.

Construction of the soil microbe library

The soil microbe library was constructed with each microbe group isolated from each soil sample. This

library includes about 500 strains of bacteria utilizing alkane, 500 strains of siderophore-like

material-producing bacteria, 1,700 strains of Actinomyces, 500 strains of Basidiomycetes.

Application of biologically active substances

Bioactive substance library has been constructed from the extracts of the soil microbe. For example,

siderophore is an iron chelating agent which microbe produce. It is thought that siderophore is able to apply

for anticancer.

Application to bioremediation

The isolated bacteria utilizing alkane are able to use for cleaning of the soil polluted with oil. In addition,

the white-rot fungi degrading high selectively lignin are able to remediate the soil polluted by dioxin or

PCB.

Application to hydrogen production

The biomass conversion from molasses, or waste of plants, to hydrogen is paid attention now. The isolated

microbes producing hydrogen are used to constract a hydrogen production system.


Bioshaker G B-200(Taitec)

Electrophoretic apparatus（GE Healthcare Japan）

Department of Bioresources Engineering,

National Institute of Technology, Okinawa College KOSEN National Institute of Technology

Research Contents

○Background and Purpose

In order to solve energy and environmental problems, I am trying

to construct the efficient synthesis process for biomass energies

such as biodiesel and bioethanol and the conversion of waste

materials to useful chemical compounds.

○Researches

Theme 1: The development of solid catalysts through mechanochemical treatments using ball mills

In the theme, the development of preparation method of

effective solid catalysts from magnesium oxides and zirconium

oxides for biodiesel synthesis through the mechanochemical

treatments using ball mills has been tried. In addition, I am

trying to develop the reaction process for the conversion of

glycerol, which is a by-product of biodiesel synthesis, to the useful

chemical compounds such as lactic acid and acrylic acid.

Theme 2: The continuous synthesis of biodiesel using a column reactor

packed with solid catalysts

The efficient synthesis process of biodiesel fuel from vegetable

oils using a simulated moving bed chromatographic reactor is

studied.

Theme 3: The hydrothermal conversion of biomass resources to useful

chemicals under high temperature and high pressure

condition

The hydrothermal conversion of biomass resources such as

bagasse and glycerol to useful chemical compounds is studied.

Biodiesel

Glyceol

Catalyst

Feed( Oil + Methanol)

Reactionproducts

Development of efficient catalysts through mechanochemical treatment

Development of efficient reaction system

OilAlcohol

Biodiesel fuel

Glycerol

Lactic acid

High temperature and high pressure reactor

Research on the conversion of waste glycerol to useful chemical

Biodegradable polymer

Packed bed reactor

250℃～350℃

Glycerol(Biomass resources)

Lactic acid(Reactant of biodegradable polymer)

Acrylic acid(Reactant of superabsorbent polymer)

Research on the Development of Efficient Reaction Process for Biomass Conversion

Name Takuya Fukumura E-mail [email protected]

Status Doctor of Engineering

Affiliations

The Society of Chemical Engineers, Japan

The Japan Institute of Energy Japanese Society for Engineering Education

Keywords Biomass energy, Low Environmental Load, Catalysis

Technical

Support Skills

・ Synthesis of biodiesel fuel and bioethanol

・ Applications of inorganic chemicals

・ Effective use of biomass resources





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Biodiversity of Oribatid mites in Ryukyu Islands

Name Wataru HAGINO E-mail [email protected]

Status Assistant Professor

Affiliations The Acarological Society of Japan, The Japanese Society of Soil Zoology, Japanese Society of Systematic Zoology

Keywords Soil fauna, Oribatid mites, Biological indicator, Ecology, Taxonomy

Technical Support Skills

ð Taxonomical study of Oribatid mites (Order Acari; suborder Oribatida). ð Development of the biological indicator used by soil organisms. ð Experience-based program on biodiversity of our familiar environment.

Research Contents Oribatid mites (Fig. 1) are small arthropods that are also called beetle mites, armored mites, or moss mites. They comprise more than 10,000 species. Most oribatid mites inhabit the soil–litter system; often, they are the dominant arthropods in temperate-forest soil. The ultimate goal of my research is to clarify the entire Japanese oribatid fauna. Toward this goal, I performed a series of taxonomic studies targeting the Oribatid fauna of subtropical regions in Japan (=Ryukyu Islands), by using morphological and molecular biological methods.

(Fig. 1) Various Oribatid mites in Japan.


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