Tocopherols and tocotrienolsTocopherols and tocotrienols...
Transcript of Tocopherols and tocotrienolsTocopherols and tocotrienols...
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Tocopherols and tocotrienolsTocopherols and tocotrienols –structure and function
Tong WangAssociate Professor, FSHN
Iowa State University
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History of tocopherolsHistory of tocopherols
In 1905, William Fletcher found if special factors were removed from food disease (Beriberi) occurredwere removed from food, disease (Beriberi) occurred Vitamin E discovered in 1922 in green leafy vegetables by Herbert Evans and Katherine Bishop Because vitamin E supported fertility, it was named tocopherol – Greek tokos = childbirth, phero = bring forth ol = alcoholforth, ol alcohol In 1936, found abundant in wheat germ oil In 1938, chemically synthesized In nature, eight compounds found to have vitamin E activity: α-, β-, γ- and δ-tocopherol; and α-, β-, γ- and δ-tocotrienol (T-3)δ tocotrienol (T 3)
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Structure of toco and T-3
AR1 = R2 = R3 = Me, α-tocopherolR1 R3 M R2 H β t h l
BR1 = R2 = R3 = Me, α-tocotrienolR1 R3 M R2 H β t t i l
,
R1 = R3 = Me; R2 = H, β-tocopherolR1 = H; R2 = R3 = Me, γ-tocopherolR1 = R2 = H; R3 = Me, δ-tocopherol
R1 = R3 = Me; R2 = H, β-tocotrienolR1 = H; R2 = R3 = Me, γ-tocotrienolR1 = R2 = H; R3 = Me, δ-tocotrienol
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Vitamin E activity and bioavailabilityActivity, IU/mg
Natural α-toco (RRR-) 1.49
Synthetic α-toco (racemic mix) 1.0
β-toco 0.6
γ toco 0 3
h l h h hi h bi il bili
γ-toco 0.3
δ-toco 0.015
α-tocopherol has the highest bioavailabilitySerum concentration of α-tocopherol controlled by liver, which preferentially resecretes only α-tocopherol via thewhich preferentially resecretes only α tocopherol via the hepatic α-tocopherol transfer protein (TTP)Other forms metabolized and excreted, so their blood and
ll l i h l h l h dcellular concentrations are much lower, thus less researched
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Biosynthesis of tocopherolsExclusively by photosynthetic organisms Chromanol ring and a 15-carbon tail condensed by h ti t (HGA) h t lt f (HPT)homogentisate (HGA) phytyltransferase (HPT)Tocotrienols are the primary form in the seed endosperm of monocots (wheat, rice, and barley), rarely p ( , , y), yin vegetative tissues Tocopherols occur ubiquitously in leaves and seeds of dicotsdicots Transgenic expression of the barley HGGT, which catalyzes the committed step of tocotrienol synthesis,
lt d i l ti f T 3resulted in accumulation of T-3Overexpression of the barley HGGT in corn resulted in an increase in total toco by six-foldy
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Biosynthesis of
HGGTtocopherols andtocotrienols
Hunter SC , Cahoon EB (2007). Enhancing vitamin E in oilseeds: unravelingin oilseeds: unraveling tocopherol and tocotrienol biosynthesis. Lipids 42 , 97–108.
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Vitamin E function – as antioxidantAntioxidants protect cells from the damaging effects of free radicalsfree radicals
Free radicals damage cells thus contributing to theFree radicals damage cells, thus contributing to the development of cardiovascular disease and cancer
Unshared electrons react rapidly with oxygen to form reactive oxygen species (ROS)
Body forms ROS endogenously and antioxidants might ll b i i i ROS d f di lprotect cells by inactivating ROS and free radicals
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Vitamin E function – cell signaling, gene and metabolic regulationgMembers in the vitamin E family possess unique biological functions
α-Tocopherol may have functions independent of its antioxidant property –it strongly inhibits platelet adhesion
γ-Tocopherol exhibit functions that are not shared by α-tocopherol, such as the anti-inflammatory effect
Epidemiological data suggests that γ-tocopherol is a better negative risk factor for certain types of cancer
Vitamin E–replete endothelial cells at the interior surface of blood vessels resist blood-cell adhering to this surface
Vitamin E modulates arachidonic acid metabolism –increasing prostacyclin that dilates blood vessels
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General function and mechanism of T-3Tocotrienols have strong neuroprotective, antioxidant, anti-cancer and cholesterol lowering properties that tocopherols do nottocopherols do not
T-3 in μM suppresses HMG-CoA reductase, the enzyme responsible for cholesterol synthesisT-3s are thought to be more potent antioxidants than tocos due to their efficient penetration into tissuesAlthough the transport T-3 is low, orally supplemented T-3 g p , y ppresults in plasma T-3 of 1 μM, a conc of an order of magnitude higher than that required to protect neurons from damageNanomolar α-T-3 prevents neurodegeneration by regulatingNanomolar α T 3 prevents neurodegeneration by regulating specific mediators of cell deathT-3 but not tocopherol, suppresses growth of human breast cancer cellscancer cells
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Activity of T-3 on breast cancer cellsActivity of T 3 on breast cancer cells
(Guthrie and Carroll, 1998)
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Activity of T-3 on breast cancer cells
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Effect of T-3 on PKCEffect of T 3 on PKC
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Effect on PKC activity is largely on membrane
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Vitamin E on coronary heart disease –(http://ods.od.nih.gov/FACTSHEETS/VITAMINE.ASP)
Ob i l di i d l f h di i h hi hObservational studies associated lower rates of heart disease with higher vitamin E intake - 90,000 nurses had 30% to 40% lower heart disease. Finnish (5,133 followed for 14 years) had decreased mortalityEffects on the heart and blood vessels of healthy women (40,000 ≥45 years of age, 600 IU E for 10 years) - no significant differences in cardiovascular events or mortalityyRandomized clinical trials showed controversial results:
The HOPE study (10,000 patients at high risk followed for 4.5 years, 400 IU/day) no fewer cardiovascular eventsIU/day) - no fewer cardiovascular eventsThe HOPE-TOO follow-up study (4,000 participants for another 2.5 years) - no protection against CHDA men's cardiovascular study (15,000 healthy physicians ≥50 years of age with 400 IU for 8 y) - no effect on major cardiovascular events
Clinical trials have not provided evidence that vitamin E preventsClinical trials have not provided evidence that vitamin E prevents cardiovascular disease. More extensive studies in younger and healthier participants taking higher doses of the supplement is needed
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Vitamin E on cancerLink between high intake of vitamin E with a decreasedLink between high intake of vitamin E with a decreased incidence of breast and prostate cancers is inconsistent:
Study on the incidence of breast cancer (18,000Study on the incidence of breast cancer (18,000 women) - no benefitA cohort study (29,000 men) - no association with y ( , )prostate cancer riskA large clinical trial (7-12 years of 400 IU E ) – no protection against prostate cancer in healthy menAn epidemiologic study showed reduced risk of death f bl ddfrom bladder cancer
The inconsistent and limited evidence precludes any d ti b t i it i E l t trecommendations about using vitamin E supplements to
prevent cancer
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Vitamin E on cognitive functionBrain has a high oxygen consumption and abundant polyunsaturated fatty acidsFree-radical can damage neurons thus contributing to cognitive g g gdecline and neurodegenerative diseases, such as Alzheimer‘sVitamin E’s protection is supported by a clinical trial (341 patients with Alzheimer on 2,000 IU/day E for 2 years) - significantly y y ) g ydelayed deteriorationVitamin E consumption was associated with less cognitive decline over 3 years of elderly, 65-102 years of ageA clinical trial in healthy older women (600 IU E for 4 years) - no apparent cognitive benefits Another trial (769 with cognitive impairment, 2,000 IU/day E) - no ( g p , , y )significant differences in Alzheimer rate
Therefore, most research results do not support the use of vitaminTherefore, most research results do not support the use of vitamin E to maintain cognitive performance
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Natural vs synthetic tocos, and health risks from excessive vitamin Ed-α-Tocopherol (stereoisomeric) vs dl-α-tocopherol (mixture of 8 stereoisomers)
High doses of α-tocopherol supplements may cause hemorrhage and interrupt blood coagulation in animals
Increased risk of hemorrhagic stroke in participants taking α-tocopherol
One meta-analysis of randomized trials found an increased risk of death at doses of ≥400 IU/day
The implications of these analyses for the potential adverse effects of high-dose vitamin E are unclear – participants middle-aged or g p p golder and with chronic diseases. More investigation is needed
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Selected food sources of vitamin E (α-tocopherol)Selected food sources of vitamin E (α tocopherol)
Food Milligrams (mg) /servingWh il 1 bl 20 3Wheat germ oil, 1 tablespoon 20.3Almonds, dry roasted, 1 ounce 7.4Sunflower seeds dry roasted 1 ounce 6 0Sunflower seeds, dry roasted, 1 ounce 6.0Hazelnuts, dry roasted, 1 ounce 4.3Peanuts, dry roasted, 1 ounce 2.2Sunflower oil, 1 tablespoon 5.6Corn oil, 1 tablespoon 1.9S b il 1 bl 1 1Soybean oil, 1 tablespoon 1.1Spinach, boiled, ½ cup 1.9
RDA for vitamin E (alpha-tocopherol) ranges between 4 mg (6 IU) for newborn to 15 mg (22.4 IU) for 14 and older
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Tocopherols and T-3 as antioxidants in bulk oil A comparative studybulk oil – A comparative study
1. Mechanism of antioxidation is free radical resonance stabalization
2. Antioxidant potency is δ > γ > α
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Sources of tocotrienols (T-3)
Palm Rice
Vegetable oilsPalm OilRice BranRice BranBarleyOat Wheat Germ
Corn (GMO)
S / t t i lSource:/www.tocotrienol.org
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Toco concentration of selected oils Tocopherols (ppm) Tocotrienols (T-3, ppm)
Sources alpha beta gamma delta alpha beta gamma deltaTotal T-3
Total Allp g p g
Palm Oil 152 - - - 205 - 439 94 738 890 Rice bran 324 18 53 - 236 - 349 - 585 980 Barley 350 50 50 - 670 120 120 - 910 1,360Oat 180 20 50 50 180 30 210 510Oat 180 20 50 50 180 - 30 - 210 510Wheat Germ 1,179 398 493 118 24 165 - - 189 2,377 Corn 282 54 1,034 54 49 8 161 6 224 1,648Corn Control 420 26 677 25 189 0 89 5 283 1,430CornCorn HGGT 249 28 607 58 664 19 3,512 520 4,715 5,657
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Oxidative stability of corn oil with elevated toco andOxidative stability of corn oil with elevated toco and T-3 - Analysis of oxidation products
Primary oxidation productDevelopment of hydroperoxides @ 60oCM d b id l (PV)Measured by peroxide value (PV)
Secondary products Secondary products Smaller volatile compounds from FA cleavage Measured by conductivity using an OSI Instrument
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Model oil system - Toco & T-3 added to purified oil
St i d RBD C Oil ik d ithStripped RBD Corn Oil spiked with:
αToco
αT 3
Concentration (ppm):
100250αT-3
δToco
δT 3
2507002 000 δT-3
γT-3 2,000 5,000
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Peroxide Value of Crude Corn Oil at 60OCPeroxide Value of Crude Corn Oil at 60OC
120
140
2005 ControlR2 = 0 9826R2 = 0.9962R2 = 0.9976
100
2005 HGGT
2006 Control
2006 HGGT
R = 0.9826R2 = 0.9972
60
80
PV
20
40
ΔPV/day 2005 2006 Control 11.96 ± 1.06 9.19 ± 0.34HGGT 9 83 ± 0 32 8 48 ± 0 27
00 1 2 3 4 5 6 7 8 9 10
Day
HGGT 9.83 ± 0.32 8.48 ± 0.27
ay
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Peroxide Value (PV) of Corn Oil at 60OC with ( )Alpha Tocotrienol added
180
200
100 ppm
140
160 250 ppm
700 ppm2,000 ppm
80
100
120
PV
5,000 ppm
Control
40
60
0
20
0 1 2 3 4 5DDay
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P id V l f C Oil t 60OC ithPeroxide Value of Corn Oil at 60OC with Alpha Tocopherol Added
160
180
100 ppm
120
140
160 100 ppm250 ppm700 ppm2000 ppm
80
100
PV
2000 ppm5000 ppmControl
40
60
0
20
0 1 2 3 4 5
Day
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Effect of toco and T-3 on oil oxidation
Change in average daily peroxide value over five days in stripped Change of PV/day of corn oil with added tocols at 60OC in the darkChange of PV/day of corn oil with added tocols at 60 C in the dark ΔPV/day α Toco αT-3 δ Toco δ T-3 γ T-3 100 ppm 6.89 ± 0.17 6.73 ± 0.02 5.34 ± 0.08 7.26 ± 0.52 5.43 ± 0.58pp250 ppm 8.87 ± 0.09 8.37 ± 0.70 4.98 ± 0.14 6.88 ± 0.24 5.01 ± 0.33 700 ppm 13.51 ± 0.10 12.85 ± 0.64 5.21 ± 0.10 6.42 ± 0.46 4.73 ± 0.56
2,000 ppm 20.68 ± 0.59 25.43 ± 1.02 4.02 ± 0.08 5.48 ± 0.46 7.26 ± 0.43 5 000 ppm 30 28 ± 0 81 38 35 ± 2 04 4 63 ± 0 05 5 36 ± 0 38 5 25 ± 0 085,000 ppm 30.28 ± 0.81 38.35 ± 2.04 4.63 ± 0.05 5.36 ± 0.38 5.25 ± 0.08 Control = Stripped control = 10.09 ± 0.35 ΔPV/day
Dolde and Wang, 2009
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Oil stability index - secondary oxidation products
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OSI - induction time @ 100OC@
OSI Induction Period Amount/Hours
14
16
10
12
Hou
rs
8
H
AT3Alpha TocoDT3
4
6
0 1,000 2,000 3,000 4,000 5,000 6,000
Delta TocoGT3
(ppm)
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Conclusions of in vitro antioxidant study in bulk oilConclusions of in vitro antioxidant study in bulk oilAntioxidant properties of tocotrienols (T-3) are similar to those of tocopherols in vitro
At higher concentrations, alpha is a less effective antioxidant than delta and gammag
All homologues inhibited secondary oxidative products even at very high concentrations (5,000 ppm) but with diminishing ve y g co ce t at o s (5,000 pp ) but w t d s geffectiveness
Alpha tocols propagated primary oxidation at concentrations asAlpha tocols propagated primary oxidation at concentrations as low as 700 ppm and pro-oxidant effects were increased with higher concentrations
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C l i kConclusion remarks
Tocopherols represent one of the most fascinating natural compounds that have the potential to influence a broad range of human health and diseaserange of human health and disease
The current state of knowledge warrants study of the lesserThe current state of knowledge warrants study of the lesser known forms of vitamin E, i.e. tocotrienols, that have significant biological functions