Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.
Transcript of Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.
![Page 1: Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649e2e5503460f94b1e8a4/html5/thumbnails/1.jpg)
Tissue Engineering
Lecture 19, 4/16/15Paper ReviewCell Origami
![Page 2: Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649e2e5503460f94b1e8a4/html5/thumbnails/2.jpg)
What is the motivation of this study?•Origami, the traditional Japanese art of paper folding, has remained popular over the centuries because it enables the production of various three-dimensional (3D) sculptures simply by folding two-dimensional (2D) sheets.
•This paper describes a method of generating three-dimensional (3D) cell-laden microstructures by applying the principle of origami folding technique and cell traction force (CTF)
•Cells naturally exert a contractile force [24], known as the cell traction force (CTF), that is generated by actomyosin interactions and actin polymerization, and pulls toward the center of the cell body (Figure 1A).
![Page 3: Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649e2e5503460f94b1e8a4/html5/thumbnails/3.jpg)
Figure 1a-c: Jemima Lamothe
![Page 4: Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649e2e5503460f94b1e8a4/html5/thumbnails/4.jpg)
Figure 1d-g: Robert Gaspardi
MPC
![Page 5: Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649e2e5503460f94b1e8a4/html5/thumbnails/5.jpg)
![Page 6: Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649e2e5503460f94b1e8a4/html5/thumbnails/6.jpg)
Figure 2: Thanh Nguyen
![Page 7: Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649e2e5503460f94b1e8a4/html5/thumbnails/7.jpg)
Figure 3: Michael Grunwald
![Page 8: Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649e2e5503460f94b1e8a4/html5/thumbnails/8.jpg)
Movies!
•Movie S1
![Page 9: Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649e2e5503460f94b1e8a4/html5/thumbnails/9.jpg)
• No flexible joint
• A: Cells seeded on to microplate exhibit maximum angle when cells contact
• B: Before and after phase contrast images of microplates
• Angle depends directly on number of cells and not microplate thickness
• Scale bar: 50 μm
Figure 4a-b: Cody Siroka
![Page 10: Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649e2e5503460f94b1e8a4/html5/thumbnails/10.jpg)
Figure 4c-e: Brittany Shepler
![Page 11: Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649e2e5503460f94b1e8a4/html5/thumbnails/11.jpg)
Movies!
•Movie S2
![Page 12: Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649e2e5503460f94b1e8a4/html5/thumbnails/12.jpg)
Figure 5: Eric Stowe
![Page 13: Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649e2e5503460f94b1e8a4/html5/thumbnails/13.jpg)
Movies!
•Movies S3-5
![Page 14: Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649e2e5503460f94b1e8a4/html5/thumbnails/14.jpg)
Figure 6: Kyle Pariseau
![Page 15: Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649e2e5503460f94b1e8a4/html5/thumbnails/15.jpg)
Movies!
•Movie S6
![Page 16: Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649e2e5503460f94b1e8a4/html5/thumbnails/16.jpg)
Conclusions, Perspectives•Authors used cell traction to drive the folding of 2D sheets into 3D cell-laden microstructures (Cell origami)
•They could make cubes, soccer balls, and tubes.
•They propose this technique could be applied to make devices such as stents/grafts, and as new 3D environments in which to study cells.
![Page 17: Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami.](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649e2e5503460f94b1e8a4/html5/thumbnails/17.jpg)
Next week!•Last paper is next week.
• I am swapping it out!! Don’t read it yet!!
• I’ll email out the paper and modified figure assignments ASAP.
•First presentations are next Thursday!!