This Monthly Progress Report provides a summary of JBEI’s ...€¦ · JBEI contacts Jay Keasling,...
Transcript of This Monthly Progress Report provides a summary of JBEI’s ...€¦ · JBEI contacts Jay Keasling,...
DOE Bioenergy Research Center (BRC) - Joint BioEnergy Institute
Monthly Progress Report – January 2015
Fiscal Year: 2015
Year / Period: Year 8 / Month 4 / January 1 - 31, 2015
Department of Energy’s Joint BioEnergy Institute is supported by the US Department of Energy, Office of Science, Office of Biological and Environmental Research, through Contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the US Department of Energy.
JBEI contacts Jay Keasling, Chief Executive Officer, [email protected], 510-495-2620 Nick Everson, Chief Operating Officer, [email protected], 510-486-6268
Executive Summary This Monthly Progress Report provides a summary of JBEI’s activities for the month of January 2015, fourth month of fiscal year 2015:
Item Count Highlights
Publications in Print 7 "Engineering temporal accumulation of a low recalcitrance polysaccharide leads to increased C6 sugar content in plant cell walls"
Publications in Press 4 "Scale-up of Ionic Liquid Based Fractionation of Single and Mixed Feedstocks"
Media Citations 10 Media highlight: U.S. Agriculture Secretary Tom Vilsack Visited JBEI
Patent Applications 2 “Generation of heritable chimeric plant traits”
Licenses 1 Bailment/License for invention # 2014-155 “Plasmids for Ralstonia Synthetic Biology Toolbox”
Presentations Given 17 Presentations were given at the “CSU Annual Biotechnology Symposium” in San Francisco, CA and at “Lignofuels 2015” in, Madrid, Spain.
On-Site Seminars 13 Prof. Anne Osbourn, Norwich Research Park Industrial Biotechnology and Bioenergy Alliance, John Innes Centre, Norwich Research Park
Tours & Visits 6 California Energy Commission and U.S. Agriculture Secretary Tom Vilsack
Industry Interactions 16 Interactions with Arzeda and Technology Holding LLC
FY15 Milestones On track. Despite their delay, the milestones below are expected to meet their deadlines:
Feedstocks - “Heterologous screening of transferases to promote in planta design of polysaccharides (SB)”
Fuels Synthesis - “Improve overall xylose utilization capability in S. cerevisiae strains and examine performance in industrially relevant yeast strains.”
Technology - “Structural characterization of Fatty Acid Synthase and other large complexes by single particle cryo electron microscopy”
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Publications
In Print
Eudes, A., Sathitsuksanoh, N., Baidoo, E. E., George, A., Liang, Y., Yang, F., Singh, S., Keasling, J. D., Simmons, B. A., & Loque, D. (2015). "Expression of a bacterial 3-dehydroshikimate dehydratase reduces lignin content and improves biomass saccharification efficiency". Plant Biotechnol J. doi, 10.1111/pbi.12310 http://www.ncbi.nlm.nih.gov/pubmed/25583257
George, K. W., Alonso-Gutierrez, J., Keasling, J. D., & Lee, T. S. (2015). “Isoprenoid Drugs, Biofuels, and Chemicals-Artemisinin, Farnesene, and Beyond”. Adv Biochem Eng Biotechnol (2015 Jan 11 ed.): Springer Berlin Heidelberg.1
Lupoi, J. S., Healey, A., Singh, S., Sykes, R., Davis, M., Lee, D. J., Shepherd, M., Simmons, B. A., & Henry, R. J. (2015). "High-Throughput Prediction of Acacia and Eucalypt Lignin Syringyl/Guaiacyl Content Using FT-Raman Spectroscopy and Partial Least Squares Modeling". BioEnergy Research. doi, 10.1007/s12155-015-9578-1 http://link.springer.com/article/10.1007/s12155-015-9578-1
Tanger, P., Vega-Sánchez, M. E., Fleming, M., Tran, K., Singh, S., Abrahamson, J. B., Jahn, C. E., Santoro, N., Naredo, E. B., Baraoidan, M., Danku, J. M. C., Salt, D. E., McNally, K. L., Simmons, B. A., Ronald, P. C., Leung, H., Bush, D. R., McKay, J. K., & Leach, J. E. (2015). "Cell Wall Composition and Bioenergy Potential of Rice Straw Tissues Are Influenced by Environment, Tissue Type, and Genotype". BioEnergy Research, 1-18. doi, 10.1007/s12155-014-9573-y http://dx.doi.org/10.1007/s12155-014-9573-y
Vega-Sanchez, M. E., Loque, D., Lao, J., Catena, M., Verhertbruggen, Y., Herter, T., Yang, F., Harholt, J., Ebert, B., Baidoo, E. E., Keasling, J. D., Scheller, H. V., Heazlewood, J. L., & Ronald, P. C. (2015). "Engineering temporal accumulation of a low recalcitrance polysaccharide leads to increased C6 sugar content in plant cell walls". Plant Biotechnol J. doi, 10.1111/pbi.12326 http://www.ncbi.nlm.nih.gov/pubmed/25586315
Velasquez, S. M., Ricardi, M. M., Poulsen, C. P., Oikawa, A., Dilokpimol, A., Halim, A., Mangano, S., Denita Juarez, Silvina P., Marzol, E., Salgado Salter, J. D., Dorosz, J. G., Borassi, C., Möller, S. R., Buono, R., Ohsawa, Y., Matsuoka, K., Otegui, M. S., Scheller, H. V., Geshi, N., Petersen, B. L., Iusem, N. D., & Estevez, J. M. (2014). "Complex Regulation of Prolyl-4-Hydroxylases Impacts Root Hair Expansion". Molecular Plant (0). doi, 10.1016/j.molp.2014.11.017 http://www.sciencedirect.com/science/article/pii/S16742052140003802
Zuo, S., Zhou, X., Chen, M., Zhang, S., Schwessinger, B., Ruan, D., Yuan, C., Wang, J., Chen, X., & Ronald, P. C. (2014). "OsSERK1 regulates rice development but not immunity to Xanthomonas oryzae pv. oryzae or Magnaporthe oryzae". J Integr Plant Biol, 56(12), 1179-1192. doi, 10.1111/jipb.12290 http://www.ncbi.nlm.nih.gov/pubmed/252662703
1 Publisher does not allow for acknowledgments. 2 Publications does now acknowledge JBEI funding. Author has requested revision. 3 Idem.
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In Press
Li, C., Tanjore, D., He, W., Wong, J., Gardner, J. L., Thompson, V. S., Yancey, N. A., Sale, K., Simmons, B. A., & Singh, S. (2015). "Scale-up of Ionic Liquid Based Fractionation of Single and Mixed Feedstocks". BioEnergy Research.
Shi, J., George, K. W., Sun, S., He, W., Li, C., Stavila, V., Lee, T. S., Simmons, B. A., & Singh, S. (2015). "Impact of pretreatment technologies on saccharification and isopentenol fermentation of mixed lignocellulosic feedstocks". BioEnergy Research.
Lopez-Ortega, M. G., Chavez-Carvayar, J. A., Stavila, V., Cheng, G., Singh, S., Simmons, B. A.,& Pérez-Pimienta, J. (2015). "Characterization of agave bagasse ad a function of ionic liquid pretreatment". Biomass & Bioenergy.
Papa, G., Rodriguez, S., George, A., Schievano, A., Orzi, V., Sale, K. L., Singh, S., Adani, F., & Simmons, B. A. (2015). "Comparison of different pretreatments for the production of bioethanol and biomethane from corn stover and switchgrass". Bioresource Technology(0). doi, 10.1016/j.biortech.2015.01.121
Media Citations
Date Article Source
Jan 1 Shaking Hands With the Private Sector Innovation
Jan 9 How to Manipulate Plants to Build a Better Biofuel Scientific American
Jan 12 JBEI ready to license 4 new technologies Biofuels Digest
Jan 13 U.S. Agriculture Secretary Tom Vilsack Visits JBEI KCBS-AM
Jan 13 U.S. Agriculture Secretary Tom Vilsack Visits JBEI KQED-FM
Jan 13 U.S. Agriculture Secretary Tom Vilsack Visits JBEI KGO-AM
Jan 21 JBEI identify microbial metabolism pathway to boost terpene yields Biofuels Digest
Jan 21 Enhancing microbial pathways for biofuel production Phys.org
Jan 21 Enhancing microbial pathways for biofuel production (e) Science News
Jan 26 Breaking Down the Wall Bioenergy Connection
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Patent Applications Inventor(s)
2013-171-02, US 14/598,599 “Generation of heritable chimeric plant traits” Dominique Loque and Yan Liang
3293-03, US 14/606,976 “Yeast artificial positive feedback loop as tool to enhance multigenes metabolic pathways”
Dominique Loque
Licenses Inventor(s)
Bailment/License – Nonexclusive Agreement # 2014-0490, Kiverdi Inc., Joint BioEnergy Institute (JBEI), Lawrence Berkeley National Laboratory for invention # 2014-155 “Plasmids for Ralstonia Synthetic Biology Toolbox”
Changhao Bi, Yung Hsu Tang and Peter Su
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Presentations
Date Division Presenter(s) Title Presentation Location
Jan 5 Feedstocks Loque, D. Use of synthetic biology to improve bioenergy crops.
Glasgow University, Glasgow, UK
Jan 8-10 Fuels Synthesis
King, S.; Chen, J. C.; Hillson, N. J.; Garcia Martin, H.; Goh, E.-B.; and Beller, H. R.
Assessing strategic chromosomal knockouts to enhance methyl ketone synthesis in E. coli for biofuel production
CSU Annual Biotechnology Symposium, San Francisco, CA
Jan 9 Fuels Synthesis
Keasling, J.D. Translating Academic Innovation to Biotechnology Development,
Center for Emerging and Neglected Diseases, University of California, Berkeley, CA
Jan 20 Feedstocks Scheller, H. V. Optimizing plant cell walls for biorefinery applications - using rice and Arabidopsis as model plants
International Rice Research Institute, Los Baños, Philippines
Jan 20-22 Deconstruction Singh, S. Enabling Bio-Economy through Biofuels & Chemicals
Lignofuels 2015, Madrid, Spain
Jan 23 Deconstruction Singh, S. Development & Demonstration of an Integrated Sugar Production Platform based on Ionic Liquids,
Abengoa, Seville, Spain
Jan 23 Feedstocks Ronald, P. The rice immune receptor XA21 recognizes a tyrosine sulfated peptide from aGram-negative bacterium
Carnegie Institution for Science, Department of Plant Biology, Stanford, CA
Jan 24 Fuels Synthesis
Keasling, J. D. Engineering Microbes to Produce our Stuff
UC Emeritus Association, Berkeley, CA
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Jan 27 Deconstruction Singh, S. Enabling Advanced Biofuels & Chemicals
Institute of Catalysis, CSIS, Madrid, Spain
Jan 27 Fuels Synthesis
Keasling, J. D. Engineering Microbes for Production of Chemicals and Fuels
National University of Singapore, Singapore
Jan 29 Fuels Synthesis
Garcia Martin, H. Quantitative metabolic modeling for biofuel synthesis
Joint Genome Institute, Walnut Creek, CA
Jan 29 Deconstruction Singh, S. Green Chemistry in USA La Bioeconomy as Key for the Future Development, Milan, Italy
Jan 29 Fuels Synthesis
Beller, H. R. Exploring microbial metabolic potential of natural systems
LBNL BioSciences Expert Advisory Committee, Berkeley, CA
Jan 29 Feedstocks Mortimer, J. Synthesis and Function of Glycans in Plants
LBNL Biosciences Review, Berkeley
Jan 29-30 Feedstocks Loque, D. Use of Synthetic Biology to Develop Novel Technologies to Improve Crops
LBNL Biosciences Review, Berkeley,
Jan 30 Fuels Synthesis
Hillson, N. J. Enhancing the Design-Build-Test-Learn Cycle for Metabolic Engineering
LBNL BioSciences Expert Advisory Committee, Berkeley, CA,
Jan 31 Feedstocks Jing, B. GONST1 and GONST2 are GIPC-specific GDP-sugar Transporters
Gordon Research Seminar on Plant Lipids, Galveston TX
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Onsite Seminars
Date Type Presenter Title Affiliation
Jan 13 General Seminar Deconstruction Guest
Jing Ke Progress towards Biological Pretreatment – Understanding Varied Lignin Modifying Mechanisms in Natural Systems
Postdoc Interview Candidate
Jan 14 JBEI Seminar Series Deepak Dugar Bio-based processes for high performance polymers: Development and commercialization
Cyclotron Road, LBL
Jan 14 Group Seminar Victor Chubukov Thermodynamics of Cofactor Engineering
Fuels Synthesis Division
Jan 14 Group Seminar Lara Rajeev Regulatory systems in D. vulgaris: nitrite-sensing and tungstate-sensing factors
Fuels Synthesis Division
Jan 16 General Seminar Fuels Synthesis Guest
Prof. Anne Osbourn Unlocking plant metabolic diversity Director, Norwich Research Park Industrial Biotechnology and Bioenergy Alliance, John Innes Centre, Norwich Research Park
Jan 21 Group Seminar Justin Hsia Design and Engineering of Pattern Formation in Gene Expression in E. coli
Fuels Synthesis Division
Jan 21 Group Seminar Sean Poust Fuels Synthesis Division
Jan 22 General Seminar Fuels Synthesis Guest
Professor Dong-Yup Lee Integrated multi-omics profiling and systems modeling for characterizing and designing microbial, plant and mammalian
Department of Chemical and Biomolecular Engineering, National University of Singapore (NUS)
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cells
Jan 22 General Seminar Deconstruction Guest
Timo Schuerg A cell biology and genetics approach to uncover the machinery and mechanisms of cellulase secretion in the model filamentous fungus Neurospora crassa.
Postdoc Interview Candidate
Jan 23 General Seminar Deconstruction Guest
Sara Saedinia Laminated Microfluidic Coupon Systems for Biological Applications
Postdoc Interview Candidate
Jan 28 Group Seminar Anna Lechner Engineering bacterial polyketide synthases in S. cerevisiae
Fuels Synthesis Division
Jan 28 Group Seminar Chris Petzold New developments in JBEI Proteomics
Technology Division
Jan 28 JBEI Seminar Series Nat Echols Automating biological crystallography data analysis for high-throughput applications in Phenix
Fuels Synthesis Division
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Tours and VIP Visits at JBEI
Date Company Attendees
Jan 7 California Energy Commission Commissioner Janea Scott, Jim Bartridge, Michelle Chester and O'Shea Bennett
Jan 13 USDA Tom Vilsack, Sec. of Agrictulture; Matthew McKenna, Special Advisor and Matt Paul, Director of Communications
Jan 16 Zhongguancun Science Park, China Xiaofeng Sun and Administrative Committee Delegation
Jan 16 Norwich Research Park Industrial Biotechnology and Bioenergy Alliance, John Innes Centre, Norwich Research Park, UK
Anne Osbourn, Director
Jan 16 La Ka Shing Foundation Solina Chau, Director
Jan 27 Piedmont High 32 students
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Industry Interactions
Date Company / Contact
Jan 12 ALS Biosciences / Sebastián Scaparro
Area of interest: ABPDU
Jan 13 Monsanto Growth Ventures / John Hamer
Area of interest: IAC and BOB
Jan 13 Cooley LLC / Fred Dorey
Jan 15 Futuragene / Daniel Siegel
Area of interest: Reporting, potential new technology areas
Jan 16 Norwich Research Park Industrial Biotechnology & Bioenergy Alliance / Prof. Anne Osbourn
Jan 16 Arzeda / Andrew Ban and Daniela Grabs
Area of interest: DOE TTO SBIR Grant
Jan 21 Technology Holding LLC / Mukund Karanjikar
Area of interest: STTR
Jan 26 Mass Insight / Tom Gilman
Area of interest: License negotiation
Jan 27 Ajinomoto / Erika Yoshida
Area of interest: Preparation for Ajinomotoo visit
Jan 27 Riffyn / Timothy Gardner
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Area of interest: Negotiating procurement agreement
Jan 28 DSM Venturing / Greg McParland
Jan 29 Virdia / Robert Jansen and Maya Skubatch
Area of interest: License agreement
Jan 31 Kiverdi / Lisa Dyson
Area of interest: Bailment agreement
Jan (multiple)
Total / Florence Mingardon, Angelique Chanal, Olivier-A. Pernet, Hubert Le-Gallic and Renaud Maillard
Area of interest: Option negotiation
Jan (multiple)
Afingen / Ai Oikawa and Henrik Scheller
Area of interest: Option agreements
Jan (multiple)
Technology Holding LLC / Mukund Karanjikar
Area of interest: STTR
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Milestones
Feedstocks Division
Area Initiatives Milestone/Deliverables Due % Complete
Plant Cell Wall Biosynthesis and Its Regulation
Novel acetyl and feruloyltransferase activities characterized (CWB) Sept. 2015 32
Genetic characterization of mutant candidates from rice stem saccharification screen (GG)
Sept. 2015 32
Correlation between cell wall modification and environmental interactions investigated (GG)
Sept. 2015 32
Characterization of novel nucleotide sugar transporters (SB) Sept. 2015 40
Characterization of novel GT activities (SB) Sept. 2015 40
Lignin and Hemicellulose Modification for Fuels Production
Evaluation of engineered plants with new lignin traits (including analysis with the Deconstruction and Technology Divisions) (CWE)
Sept. 2015 40
Transgenic plants with dominant repressors of hemicellulose biosynthesis characterized (CWB)
Sept. 2015 28
Nucleotide sugar transporters used for cell wall engineering (CWB) Sept. 2015 34
Optimization of translation control technologies to improve tissue-specific expression (CWE)
Sept. 2015 33
Analysis of stacked traits in Arabidopsis (CWE) Sept. 2015 30
Heterologous screening of transferases to promote in planta design of polysaccharides (SB).
Sept. 2015 10
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Milestones
Feedstocks Division (continued)
Area Initiatives Milestone/Deliverables Due % Complete
Engineering of Modifications in Different Plant Species
Cell wall related genes in switchgrass functionally annotated, integrated into phylogenomics databases, and integrated with Kbase (GG)
Sept. 2015 32
Resequencing of Kitaake mutants (with JGI) and a database set up for hi throughput in silico screening (GG).
Sept. 2015 32
Translation of best technologies developed in Arabidopsis to other plant species (CWE)
Sept. 2015 35
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Milestones
Deconstruction Division
Area Initiatives Milestone/Deliverables Due % Complete
Biomass Pretreatment
Predict and develop task specific ILs for selective lignin or cellulose dissolution and low temperature pretreatment
May 2015 50
Complete characterization of lignin streams from various IL pretreatment process configurations
July 2015 40
Design, synthesis and assessment of lignin and hemicellulose derived IL mixtures for mixed and engineered feedstock pretreatment
Aug. 2015 40
Use multi-scale simulations strategies (from first principle quantum mechanics to force field based) to understand interactions between biomass derived ILs and lignocellulosic biomass
Sept. 2015 30
Enzyme Optimization
Develop an assay for screening lignin degrading enzymes on insoluble lignin and begin populating a lignin degrading enzyme database (LigDB).
Feb. 2015 90
Expand our database of ionic liquid tolerant enzymes, particularly to include enzymes from the GH3, GH5, GH6, GH9, GH10, AA9 and AA10 families.
May 2015 70
Improve the overall sugar yields of our existing thermo and ionic liquid tolerant multi-component enzyme mixture by including enzymes for hydrolysis of both cellulose and hemicellulose and by including LPMOs.
July 2015 35
Demonstrate using directed evolution to engineer enhanced stability -glucosidase in up to 20% [C2mim][OAc].
Sept. 2015 40
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Milestones
Deconstruction Division (continued)
Area Initiatives Milestone/Deliverables Due % Complete
Microbial Communities
Complete characterization of active component of JTherm March 2015 80
Establish complementary thermophilic cellulase cocktail focusing on crystalline substrates
July 2015 50
Identify five bacterial strains capable of metabolizing mixtures of aromatics from pretreatment-derived lignin streams
Sept. 2015 50
Fungal Biotechnology
Design new expression systems based on recent “omics” discoveries in regard to promoters and assess heterologous GH expression
Feb. 2015 90
Initiate ChIP-Seq analysis of histone modifications in A. niger aimed at identifying epigenetic determinants of high productivity
April 2015 55
Optimize cultivation conditions for heterologous enzyme production, including temperature and pH, and initiate ‘omic analysis of optimal vs. baseline conditions
July 2015 45
Validate candidate genes for hyperproduction phenotypes through gene deletion and/or overexpression
Sept. 2015 40
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Milestones
Fuels Synthesis Division
Area Initiatives Milestone/Deliverables Due % Complete
Discovery of Novel Hydrocarbon Biochemistries
In vitro, anaerobic testing of toluene biosynthesis candidates Sept. 2015 33
In vitro, anaerobic testing of ladderane biosynthesis candidates Sept. 2015 33
Optimization of Hydrocarbon Biosynthetic Pathways
Improve methyl ketone production in E. coli with NIMS-aided HT screening and metabolic modeling
Sept. 2015 33
E. coli pathway engineering for isopentenol production both in aerobic and anaerobic conditions
Sept. 2015 35
Genes encoding isoprenoid pathway integrated into E. coli chromosome
Sept. 2015 33
Three enzymes in S. cerevisiae glycolytic pathway replaced and impact on fatty acid-derived fuel tested
Sept. 2015 33
Type 1 fatty acid synthases from several organisms expressed in E. coli and production of fatty acid-based fuels tested
March 2015 100
Develop analytical method for LC-MS of MEV and DXP intermediates April 2015 60
Host Engineering Improve overall xylose utilization capability in S. cerevisiae strains and examine performance in industrially relevant yeast strains.
Sept. 2015 20
E. coli membrane editing: With JGI, generate 10-20 single and double mutants and test improvement in expression of beneficial tolerance pump and corresponding tolerance to toxic fuels.
Sept. 2015 39
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Milestones
Fuels Synthesis Division (continued)
Area Initiatives Milestone/Deliverables Due % Complete
Synthetic Bio Informatics
Codon optimization tool (GeneDesign) integrated into the DIVA platform.
Sept. 2015 34
Clonal sequence validation (Sanger and/or MiSeq) pipeline implemented and integrated with DIVA/ICE
Sept. 2015 34
Quantitative Metabolic Modeling for Host and Pathway Engineering
Usage of EDD, MvT and 2S-13C MFA to improve production of fatty acids in S. cerevisiae.
Sept. 2015 80
Perform flux analysis of S. cerevisiae sip1 strain June 2015 90
Implement interactive metabolic engineering on MvT Sept. 2015 65
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Milestones
Technology Division
Area Initiatives Milestone/Deliverables Due % Complete
High Throughput Screening
Support the optimization of T and IL tolerant cellulose/hemicellulase cocktail using HT-NIMS
Sept. 2015 35
Apply HT NIMS screen for fatty acids based fuel molecules (e.g. FAMK) in collaboration with the Fuels Division.
Sept. 2015 35
Implement software to automate unit operations (e.g., droplet dispensing, routing, merger, and sorting) on an integrated droplet chip
Sept. 2015 33
Demonstrate microfluidic droplet platform for integration of DNA assembly, transformation of cells, and culturing
July 2015 40
Proteomics Develop rapid untargeted proteomics methods for relative quantification of >800 E. coli and S. cerevisiae proteins
Sept. 2015 40
Develop absolute quantification targeted proteomic assays for 150 S. cerevisiae proteins
Sept. 2015 40
Implement membrane proteomics assays to enable E. coli membrane editing with Fuels Synthesis Division
Sept. 2015 50
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Milestones
Technology Division (continued)
Area Initiatives Milestone/Deliverables Due % Complete
Physical Characterization
SEM and TEM analysis of genetically altered feedstock biomass (including lignin and protein mapping) with Feedstocks and Deconstruction Divisions
Sept. 2015 33
Electron tomographic analysis of plant cell walls of engineered feedstocks, and CAD model generation for mechanical properties simulation.
Sept. 2015 33
Structural Biology Test expression of multiple GT clones in insect cell platform to produce quantities of protein for crystallization trials
Sept. 2015 50
Characterize lignin degrading enzymes in collaboration with GLBRC Sept. 2015 85
Structural characterization of Fatty Acid Synthase and other large complexes by single particle cryo electron microscopy
Sept. 2015 20
Synthetic Bio Informatics
EDD further developed to support enzyme characterization data and workflows, with Deconstruction Division
Sept. 2015 34
EDD further developed to support genetic expression tool data and workflows, with Fuels Synthesis
Sept. 2015 34
BioCad tools enabled to query ICE and EDD for enzymes and expression tools satisfying specified performance characteristics
Sept. 2015 34
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Milestones
Operations Management Division
Area Initiatives Milestone/Deliverables Due % Complete
Intellectual Property Management
Generate >10 inventions Sept. 2015 71
Educate researchers about the tech transfer process Sept. 2015 35
Licensing Increase number of patent applications or inventions licensed or optioned and/or licensing agreements executed relative to the average of years 1 - 7.
Sept. 2015 34
Start-up Company Creation
Identify any IP or portfolios of IP that could lead to a start-up company and create an internal commercialization team to develop an initial go-to-market plan around that IP.
Sept. 2015 33
Maintain regular JBEI on-site office hours for the Berkeley Lab entrepreneur adviser.
Sept. 2015 33
Business Development Outreach
Promote each JBEI invention to at least 700 contacts in the biofuels industry.
Sept. 2015 34
Conduct meetings with at least fifteen companies each year, at least one-third of which are new to JBEI
Sept. 2015 35
Industry Advisory Committee
Hold an annual meeting of the IAC to inform JBEI research of industry concerns and technology bottlenecks.
Sept. 2015 26
Industry Partnership Program
Strengthen relationships with current partners and pursue collaborations with new industry partners in areas of complementary research.
Sept. 2015 34
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Milestones
Operations Management Division (continued)
Area Initiatives Milestone/Deliverables Due % Complete
Annual Scientific Advisory Meeting
Hold the annual meeting and report to DOE on the findings of the committee
Sept. 2015 33
Annual JBEI Conference
Annual JBEI Retreat Sept. 2015 44
Human Resources Staffing for year 8 complete Sept. 2015 33