Listen to the Sound of Science.Discover www.zeiss.com/functionality

Think of Highest Functionality

Compose Your Own Personal Scope

Cold Spring Harbor Laboratory is renowned for its teaching of biomedicalresearch techniques. For decades, participants in its celebrated, hands-on courses

and users of its laboratory manuals have gained access to the most authoritative andreliable methods in molecular and cellular biology. Now that access has moved online.

Visit Cold Spring Harbor Protocols today and discover a rich, interactive source of newand classic research techniques. The site is fully searchable, with many tools that canbe customized by users, including topic-based alerting and personal folders. Througha web-based editorial process, users also have the opportunity to add refereed com-ments to each protocol. Links in the online protocols offer additional resources, andstep-by-step instructions print out in a convenient form, complete with materials,cautions, and troubleshooting advice. Each protocol is citable and presented and edit-ed in the style that has made Molecular Cloning, Antibodies, Cells, and many otherCold Spring Harbor manuals essential to the work of scientists worldwide. The current collection of more than 1000 protocols is continuously expanded, updated,and annotated by the originators and users of the techniques.

CSH Protocols is created by Cold Spring Harbor Laboratory Press in association withHighWire Press of Stanford University.

• A new online source of trusted techniques in molecular and cellular biology

• Contains cutting-edge and classic protocols presented step-by-step with cautions and troubleshooting

• Frequently updated and annotated

• Interactive, customizable, and fully searchable

Subject Coverage

AntibodiesBioinformatics/GenomicsCell BiologyChromatographyComputational BiologyDNA Delivery/GeneTransferElectrophoresisGeneticsHigh ThroughputAnalysisImaging/MicroscopyImmunologyLaboratory OrganismsMolecular BiologyNeuroscienceNewly Added ProtocolsPlant BiologyPolymerase Chain Reaction (PCR)Proteins and ProteomicsRNA Interference(RNAi)/siRNAStem CellsTransgenic Technology

For pricing information or to request a free trial, visit the website or: Phone:1-800-843-4388 (Continental US and Canada) or 516-422-4100 (all other locations)

Fax: 516-422-4097 E-mail: cshpress@cshl.edu or Website: www.cshlpress.comWrite: Cold Spring Harbor Laboratory Press, 500 Sunnyside Blvd., Woodbury, NY 11797-2924

Cold Spring Harbor ProtocolsExecutive Editor: Dr. David Crotty • ISSN 1559-6095 / online, monthly

Available exclusively via institutional site license

Unleash the Potential of Real-Time PCR

LightCycler® Real-Time PCR Systems

The introduction of the LightCycler® Instrument in 1998 launched anew era in real-time PCR, inspiring novel applications and discovery.The versatile LightCycler® 480 System continues this tradition,unleashing the potential of real-time PCR through expanded analysiscapabilities with the power to meet evolving research demands.

� Efficiently analyze genes with High Resolution Melting: Apply this publication-proven method to investigate SNPs, degree of methylation, and RNA editing.

� Detect genetic variation with confidence: Use a powerful combinationof three robust and convenient analysis methods to obtaincomprehensive results (Figure 1).

� Rapidly analyze gene expression data: Choose from a broad range ofbasic and advanced quantification methods to meet your research needs.

Explore the possibilities of Real-Time PCR. Visit www.lightcycler480.com to learn more about the LightCycler® 480System and register for updates about special events celebrating the 10th Anniversary of the LightCycler® System.

www.roche-applied-science.com

Figure 1: Confidently detect genetic variation with the powerful combination of LightCycler® 480 Systemanalysis modules for:

A. Melting Curve AnalysisB. Endpoint GenotypingC. High Resolution Melting

A

B

C

For general laboratory use. Not for use in diagnostic procedures.

This LightCycler® 480 Real-Time PCR System is licensed under U.S. Patent6,814,934 and corresponding claims in its non-U.S. counterparts and oneor under more of U.S. Patents Nos. 5,038,852, 5,656,493, 5,333,675, orcorresponding claims in their non-U.S. counterparts, for use in life science,by implication or by estoppel under any patent claims or for any otherimplication. The product is covered in-part by US 5,871,908, co-exclusivelylicensed from Evotec OAI AG. Parts of the Software used for theLightCycler® 480 System are licensed from Idaho Technology Inc., Salt LakeCity, UT, USA.

LIGHTCYCLER and HRM are trademarks of Roche. Other brands or product names are trademarks of their respective holders.

© 2008 Roche Diagnostics GmbH. All rights reserved.

Roche Diagnostics GmbHRoche Applied Science68298 Mannheim, Germany

75 years at the frontiers of science: www.grc.org

Gordon Research Conferences

Signaling by Adhesion Receptors (Graduate Research Seminar) NEW!

June 28-29, 2008 Mount Holyoke College, South Hadley, MA

Signaling by Adhesion Receptors (GRC)

June 29 - July 4, 2008Mount Holyoke College, South Hadley, MA

The Biology of Post-Transcriptional Gene Regulation

June 29 - July 4, 2008Colby College, Waterville, ME

Signal Transduction by Engineered Extracellular Matrices

July 6-11, 2008Bates College, Lewiston, ME

Plant Molecular Biology

July 13-18, 2008Holderness School, Holderness, NH

Macromolecular Organization & Cell Function

July 27 - August 1, 2008Magdalen College, Oxford, United Kingdom

Growth Factors & Signalling

August 3-8, 2008Magdalen College, Oxford, United Kingdom

Immunochemistry & Immunobiology

August 17-22, 2008Magdalen College, Oxford, United Kingdom

GRC is holding over 190 meetings in 2008. A few upcoming meetings that may be of particular interest to Genes & Development readers are listed below.

4th Edition

By John A. Kiernan

The chemical and physical principles of fixation, staining, and histochemistry in one volume!

Now in its fourth edition, Histological and Histochemical Methods has been expandedand updated with the latest techniques and developments within the field, whilst retainingthe details of the classic techniques still in use. The relations of chemical structures andreactions to fixation, tissue processing, staining, enzyme location, immunohistochemistryand other procedures are explained in simple, practical terms.

This book is recommended for lecturers and students in biological and biomedical disciplines using histologicaland histochemical techniques. Postgraduate researchers will find this to be the ideal laboratory companion, aswill medical laboratory technologists preparing for their professional examinations. Every histopathology laboratoryshould have a copy.

2008, 606 pp., Bibliography, Glossary, IndexPaperback: $75.00 ISBN 978-1-904842-422

CONTENTS

1. Introduction to microtechnique

2. Fixation

3. Decalcification and other treatments for hard tissues

4. Processing and mounting

5. Dyes

6. Histological staining in one or two colours

7. Staining blood and other cell suspensions

8. Methods for connective tissue

9. Methods for nucleic acids

10. Organic functional groups and protein histochemistry

11. Carbohydrate histochemistry

12. Lipids

13. Methods for inorganic ions

14. Enzyme histochemistry: general considerations

15. Hydrolytic enzymes

16. Oxidoreductases

17. Methods for soluble organic compounds of low molecular weight

18. Metal reduction and precipitation methods

19. Immunohistochemistry

20. Miscellaneous data

Bibliography

Glossary

Index

To order or request additional information:Call: 1-866-854-3301 (Continental US and Canada) Fax: 516-422-4097Email: USorders@scionpublishing.com WWW Site: www.scionpublishing.comWrite: Scion Publishing Ltd., 500 Sunnyside Blvd., Woodbury, NY 11797-2924