1 PRINCIPLE, APPLICATIONS & ADVANCES IN MASS SPECTROMETRY. MASS SPECTROMETRY.
Thermo Scientific TSQ Quantum Access MAX · From the company renowned for innovation in mass...
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Innovation, Simplicity, and Value Thermo Scientific TSQ Quantum Access MAX Food Safety Environmental Analysis Drug Discovery and Pharmacokinetics Clinical Research mass spectrometry Part of Thermo Fisher Scientific
Transcript of Thermo Scientific TSQ Quantum Access MAX · From the company renowned for innovation in mass...
Innovation, Simplicity, and Value
Thermo Scientific TSQ Quantum Access MAX
Food Safety
Environmental Analysis
Drug Discovery and Pharmacokinetics
Clinical Research
m a s s s p e c t r o m e t r y
Part of Thermo Fisher Scientific
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UNSURPASSED PRICE TO PERFORMANCE
From the company renowned for innovation in mass spectrometry,
the Thermo Scientific TSQ Quantum Access MAX is the newest
member of the successful TSQ product family.
The TSQ Quantum Access MAX™ offers several innovative features that provide real benefits:
• Superior detector response in negative ion mode for matrix intensive assays suchas the analysis of the antibiotic Chloramphenicol and steroids such as beta-estradiol
• Mass range up to m/z 3000 supports a wide array of applications
• Fast +/– switching for multi-residue analyses
• QED-MS/MS and library search facilitate simultaneous quantitation and structural confirmation at the click of a button
Simple, intuitive software makesthe system easy to use and enables faster method acceptance withoutoperator retraining on complexhybrid technology.
The TSQ Quantum Access MAXredefines versatility, making it thevalue-conscious choice for a widevariety of applications, including:pharmaceutical, environmental, food safety, clinical research, andforensics. The TSQ Quantum AccessMAX sets the benchmark for sensitivity in its class, providing unmatched specificity at an affordable price.
Thermo Scientific Accela High Speed LC and TSQ QuantumAccess MAX mass spectrometer. Accela™ LC system performsconventional and U-HPLC separations in one efficient and versatile system.
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eSIMULTANEOUS QUANTITATION AND
STRUCTURAL CONFIRMATIONQuantitation-Enhanced Data-Dependent MS/MS (QED-MS/MS)
provides significant benefits for multi-residue screening and
quantitation experiments.
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SRM Quantitation (Scan Event 1)MS/MS Confirmation (Scan Event 2)
QED-MS/MS of an Herbicide MixQED-MS/MS provides simultaneous quantitation and structural confirmation inmetabolite identification or multi-residuescreens. Chromatograms at left show a multi-residue screen of a pesticide mix using posi-tive/negative switching and QED-MS/MS.
With the ability to perform HighlySelective Reaction Monitoring (H-SRM), the MS/MS data is cleanerand less complex, making it easilysearchable against a built-in libraryfor added confirmation duringscreening assays.
A related innovation is the reversedenergy ramp (RER) feature, whichallows the user to create libraries that are searchable on TSQ Seriesuniversally. The same library can beused in a laboratory in Shanghai,
China, and in Somerset, New Jersey – this is a first for triplestage quadrupole-based MS/MSstructural identification.
Sample scan (top) andlibrary match (bottom)showing confirmationof atrazine
Sample scan
Library match
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MCPA -ESI
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Atrazine +ESI
Simazine +ESI
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HESI-II Heated Electrospray Ionization Source A design evolution, the HESI-II delivers greater desolvation and improved nozzle performance. The HESI-IIcan efficiently handle flow rates higher than 1mL/min while maintaining superior low flow ratecharacteristics with optional use of gas assisted thermal desolvation.
A cleaner nozzle profile improves electrostatic fields which results in a singleposition that works for most flow rates.
These improvements make the HESI-II the most versatile elec-trospray design on the market.
Improved heater design for betterdesolvation at higher flow rates
Contoured tip for stable low flow performance
ROBUST AND SENSITIVE IONIZATION SOURCES
FOR ROUND-THE-CLOCK PRODUCTIVITY
%RSD = 2.9%
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Robustness study on the TSQ Quantum Access MAX.300 injections of 2 pg Cabergoline in pig plasma 400 µL/min,MeOH-Water (0.1% formic), Hypersil GOLD™ C-18, 50 × 2.1 mm
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Sensitivity study on the TSQ Quantum Access MAX showing asignal-to-noise in excess of 200:1 for 50 fg/uL of Alprazolam, in rat plasma400 µL/min, MeOH-Water (0.1% formic), Hypersil GOLD C-18, 50 × 2.1 mm
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The significant improvement in quantitative per-formance is shown for 10 pg/mL of Alprazolaminjected on a Thermo Scientific Hypersil GOLDC-18 column (50 x 2.1 mm, 3 µm), using an isocraticsolvent mixture of Acetronitrile:Water (0.1% formicacid, 65:35, v/v), at a flow rate of 400 µL/min.
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ZERO CROSS-TALK COLLISION
CELL AT 2 ms DWELL TIME
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Triasulfuron 402 > 167
Metsulfuron-methyl 382 > 167
Chlorosulfuron 358 > 167
Metsulfuron-methyl 382 > 167
Chlorosulfuron 358 > 167
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Characteristic of the three pesticides–triasulfuron, metasulfuron-methyl and chlorosulfuron–used to demonstratezero cross-talk on the TSQ Quantum Access MAX. Baseline magnified × 6000 = 0.01%.
High Speed Zero Cross-TalkCollision CellA long standing tradition of TSQcollision cell design has been thefact that they have had zero cross-talk. Cross-talk usually occurs whenthe instrument is set to scan veryfast, resulting in residual signalfrom the previous SRM transition
appearing in the subsequent SRMchannel when the product ion hasthe same nominal mass. This leadsto false positive responses. The collision cell design allows the TSQ Quantum Access MAX to perform SRM scans at the fastestpossible scan speeds, while ensuring zero cross-talk.
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HIGHER PRODUCTIVITY THROUGH FAST POLARITYSWITCHING AND 3000 TIMED SRMS
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Fast Polarity SwitchingBeing able to carry out multi-residue screen-ing and quantitation simultaneously in bothpositive and negative ionization modes canreduce LC/MS analysis time by half. Thefast switching power supply of the TSQQuantum Access MAX allows posi-tive/negative switching at ≤ 25 ms, allow-ing the instrument to capture high qualitydata in positive and negative ionizationmodes simultaneously. This is of significantvalue when analyzing multiple classes ofcompounds in one sample that require ion-ization in both polarities.
3000 Timed SRMsFurther improvements in productivity arisefrom the ability of the TSQ Quantum AccessMAX to perform up to 3000 Timed SRMs (T-SRMs) in one scan event. Using T-SRMs,the mass spectrometer is able to divide allthe SRMs into individual smaller batcheswithin a fixed chromatography timescale. Inthis way, less time is wasted scanningwhere there are no peaks, resulting in bet-ter data quality and improved sensitivity dueto reduction of the cycle time and/or anincrease of the individual scan time.
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1 ng mixture of eight acid herbicides and triazines collected with polarity switching at 25 msec. A total of 36 SRMs were collected in this experiment, 16 (8 +ESI, 8 -ESI) of which areshown here.
250 pesticides in orange oil, combined positive and negative ionization modes
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THERMO SCIENTIFIC EQUAN ENVIRONMENTAL
QUANTITATION SYSTEMEQuan™ is a turnkey system incorporatingthe TSQ Series mass spectrometer for the analysis of pesticides, herbicides,antimicrobials, antibiotics, and other pharmaceuticals in water samples. EQuan’s unique on-line sample enrichmenttechnique significantly reduces analysistime – from days to minutes. Its high injection volume (1–20 mL) improves detection limits over conventional LC-MS/MS analysis.• On-line clean-up of environmental
samples results in improved throughput, sensitivity and RSDs
• Significant time savings over off-linesample cleanup and enrichment
• Enhanced resolution (H-SRM) for analyte specificity
• Simultaneous quantitation and structural confirmation with QED-MS/MS
• Extensive library of pesticides and pharmaceuticals
• Direct injection up to 20 mL
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Multi-residue pesticide analysis (10 ppt) using the TSQ Quantum Access MAXEQuan system with Accela high speed LC. All components eluted under 3 minutes(compared to 17 minutes using standard HPLC). 1 mL injection volume.
Chromatograms showing the injection of 5 and 20 mL injection volumes.The concentration of Simetryn is 1 pg/mL for both injections.
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Simplicity
THERMO SCIENTIFIC APPLICATION-SPECIFIC SOFTWARE
Xcalibur™ Data SystemXcalibur is the most powerful andflexible MS data handling systemavailable for Microsoft® Windows.®
The software provides fully auto -mated control of the TSQ Quantumseries, Thermo Scientific SurveyorLC, and Accela, acceleratingmethod development with intelli-gent optimization tools. The func-tional workspace-based instrumentcontrol view creates a simplifiedtune, calibrate, and compound optimization environment for analytical method development.
LCQUAN™ – QuantitationSoftware for RegulatedLaboratoriesLCQUAN is a secure data system for quantitative analysis. LCQUAN
provides the necessary resourcesfor importing sequence informationfrom external systems, developingmethods, reviewing data, process-ing data, reporting data, and exporting results to externalsystems; all within a 21 CFR Part 11compliant environment. LCQUAN
provides multilevel security access,giving system administrators theability to set user privileges fromfull system access to data reviewonly. LCQUAN offers the ability tosafely process data stored on network-based computing systems.
TraceFinder™ – OptimizedWorkflow for Trace AnalysisTraceFinder’s extensive menu ofpreconfigured methods and reportformats makes routine screeningand quantitation of contaminants asimple process, even for noviceusers. Choose the test, build thesample list, select the reportoptions and submit the samples foranalysis. TraceFinder’s simple point-and-click interface prompts the userthrough the steps to create meth-ods for targeted screening andquantitation.
MetWorks™ – Metabolite Identification SoftwareMetWorks streamlines the reviewof drug metabolism LC-MS/MSsamples. It simplifies the process of generating reconstructed ionchromatograms, mass spectra, andsummary reports from hundreds of MS/MS scans isolated in a typical metabolic run.
Mass Frontier™ – Spectral Management ToolsMass Frontier provides rational understanding of compound structures and a mechanistic understanding of fragmentationschemes using proprietary mass spectral interpretation algorithms.In addition, relationships withindatasets can be explored using various types of classification.
QuickQuan™ – Drug DiscoveryQuantitationQuickQuan accelerates data acqui-sition and processing for all targetcompounds in an analytical run andgenerates quantitative results auto-matically. The intelligent automa-tion of data acquisition provides atotal solution that speeds the paceof drug discovery.
QuickCalc™ – Powering HT-ADME ResearchQuickCalc software is powered byGMSU (Gubbs Mass Spec Utilities)software to enhance the data pro-cessing and results display in high-throughput Absorption, Distribution,Metabolism and Excretion (HT-ADME) studies. QuickCalcreduces the time required to discover new compounds that willenter the drug development phase.
ToxID™ – Automated ToxicologyScreening SoftwareToxID is simple and easy-to-usesoftware that substantially simpli-fies LC-MS/MS toxicology screen-ing workflows for clinical researchand forensic laboratories. Thisunique software provides powerfulcompound identification, automatedworkflows and concise reports.
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TraceFinder Software
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Signature Benefits
• TraceFinder method development software
• Built-in library of environmental and foodsafety contaminants
• H-SRM dwell time of 2 ms–Quantitatehundreds of compounds in a singlerun for multi-residue screening
• Mass range m/z 10-3000 –Smallmolecules to intact proteins
• QED-MS/MS–Simultaneous quantitationand structural confirmation
• 3000 Timed SRMs
• Zero Cross-Talk Collision Cell–Eliminate false positives
• 21 CFR Part 11 enabled software
• Fast pos/neg switching (–< 25 ms) formulti-residue assays
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