State-of-the-art performancePlug-and-spray with

Thermo Scientific EASY-Spray Technology

2|

Effortless nano electrospray ionization

Integrated design

Nano-flow LC-MS relies critically on perfect connections between

several pieces of nano-bore tubing and high-voltage electrodes

for the spray ionization. Thermo Scientific™ EASY-Spray™ technology

provides an integrated and temperature-controlled column-emitter

design using a single Thermo Scientific™ Dionex™ nanoViper™ connection

between the LC and the EASY-Spray source to remove the most

error-prone and difficult connections.

Plug-and-spray nano-electrospray

ionization combined with

industry-proven columns: the

Thermo Scientific EASY-Spray.EASY-Spray source

|3

Nano-flow LC-MS relies critically on perfect connections between

several pieces of nano-bore tubing and high-voltage electrodes

for the spray ionization. Thermo Scientific™ EASY-Spray™ technology

provides an integrated and temperature-controlled column-emitter

design using a single Thermo Scientific™ Dionex™ nanoViper™ connection

between the LC and the EASY-Spray source to remove the most

error-prone and difficult connections.

Plug-and-spray nano-electrospray

ionization combined with

industry-proven columns: the

Thermo Scientific EASY-Spray.

“ The optimization and

reproducibility achievable by

years of nanospray experience

are built into the device.

Heated UHPLC 50 cm column

chromatography was not easy

until now!”

— Paul Taylor, PhD Hospital for Sick Children, Toronto, Canada.

More than ever beforeThermo Scientific proteomics solutions are designed

to give you more than ever before:

more throughput, more sensitivity, more

separation power and more ease of use.

4|

Plug-and-Spray

Plug-and-spray nano-electrospray ionization

The EASY-Spray columns are high-tech assemblies that are carefully manufactured to the highest standards in nano-flow chromatography.

Integrated ZDV union

Spray tipIntegrated column heating (30-60 °C operating range)

The metal cylinder provides a trouble-free high-voltage connection from the source to the eluent

nanoViper fitting

Precision positioned glass emitter with uniform ID and untapered flow pathCircular design allows

column to be coiled up, accommodating

long columns.

Classic connection

nanoViper connection

|5

Power and sensor cable for EASY-Spray

columns

Simply press until it clicks into place

Temperature control display

Forward/backward adjustment knob

Excellent Spray Stability Sample: Singly charged polytyrosine peptide

MS: Thermo Scientific™ LTQ Velos™

Source setup: EASY-Spray source direct infusion emitter

Camera provides light and a convenient, magnified view of the emitter tip

20000000

18000000

16000000

14000000

12000000

10000000

8000000

6000000

4000000

2000000

00 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30

Inte

nsity

Time (min)

Achieving state-of-the-art performance from nano-flow LS-MS was never easier: simply plug-and-spray

EASY-Spray technology delivers performance, reproducibility and reliable results.

State-of-the-art performance

Outstanding reproducibility

7 BSA runs on a Thermo Scientific™ TSQ Vantage™ triple stage quadrupole mass spectrometer. Retention time precision less than 0.2% RSD.

Courtesy of Paul Taylor and Thomas Kislinger, Hospital for Sick Children, Toronto, Canada.

Reliable results every time

EASY-Spray technology provides stable performance over time to match today’s requirements for proteomics researchers.

5 10 15 20 25 30 35 40 45 50 55 60 65Minutes

020406080

1000

20406080

100

020406080

100

Rela

tive

Abun

danc

e

020406080

10034.36

575.279222.16688.8204 38.55

901.463917.21

447.7194

46.03672.401115.09

410.213626.93

514.297830.84

629.3431 799.422245.79581.335910.64

483.255851.11

799.422758.44

801.753560.59

1104.25188.98514.7664

34.58575.2802

17.55447.7194 22.46

688.8212 40.54870.4832

15.41410.2140

13.12401.2454 46.21

672.402131.92

551.782027.13

514.2982 51.01799.423046.01

581.337312.05415.7356 51.76

590.316358.61

801.7560

34.05575.2821 39.93

870.485117.11447.7207

45.64672.4036

12.77401.2462

21.93688.8231

31.38551.7839

29.19528.2762

50.37799.424612.14

452.705745.44

581.338451.03

590.317357.99

801.757110.86

483.257760.14

1104.2584

34.46575.2799

12.97401.2449

17.39447.7197 22.33

688.821340.40

870.4829 46.09672.4016

26.98514.2983

31.74551.7820

12.30452.7043

50.79799.4229

45.52652.3970

11.10420.7693

51.57590.3154

8.82430.2198

58.36801.7546

Injection #1

Injection #15

Injection #30

Injection #45

6|

Selected base peak chromatograms from 500 ng samples of E. coli digest analyzed on a Velos Pro Orbitrap™ instrument with over 45 repeat runs. A 50 μm ID × 15 cm column was used for the separation. The gradient was 5% B to 30% B in 60 min at 300 nL/min. The column heater was set at 35 °C.

Column: EASY-Spray ES801 (15 cm × 50 µm ID Thermo Scientific™ Acclaim™ PepMap™ RSLC C18, 2 µm)Peak width A: 2.5s FWHMPeak width B: 3.0s FWHM Courtesy of Karl Mechtler, Institute of Molecular Pathology (IMP), Vienna, Austria.

The combined column/sprayer fitted with nanoViper fittings eliminate dead volumes. The result: Ultra-sharp peaks (2.5-3.0 s full width half maximum) that maximize peptide coverage.

“ Right off the shelf, with absolutely no optimization, the first injection

gave me beautiful peaks with FWHM times of 2.5-3 seconds!”

— Duncan Smith, PhD Paterson Institute for Cancer Research, Manchester, England.

RT min

# of injection40

45

50

55

60

0 5 10 15 20 25

Room Temp35 ⁰C45 ⁰C55 ⁰C

0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80

Time (min)

0

20

40

60

80

100

33.29575.2820

39.35870.4839

21.27688.822311.71

601.2952 29.75629.3451

44.84672.4033

50.22799.4244

10.88452.7047

53.75759.4297

57.75801.7576

7.84508.2492 62.10

964.057771.10

415.213573.38

488.3965

Column 3

0

20

40

60

80

100

Rela

tive

Abun

danc

e

33.68575.2828

21.70688.8232 38.14

901.4670 45.13672.4035

15.89629.837312.23

601.296025.99

514.299949.68

799.4254

10.86415.7364 57.57

801.7568 61.74964.0568

71.12415.2140

75.03469.3158

Column 2

0

20

40

60

80

100

33.14575.2822

21.09688.8206

36.36740.3671

50.17799.423645.43

672.402227.24

565.791415.96

447.720611.41601.2949

58.02801.7542

9.03531.2291 62.70

964.054972.79

532.3857

Column 1Excellent inter-column reproducibility Column-to-column reproducibility

is extremely important in

proteomics, particularly when

running big experiments or

multi-site collaborations. The

exacting assembly precision

of EASY-Spray columns ensures

class leading performance on

these parameters.

Temperature control Column temperature control immediately before the MS inlet increases run to run reproducibility and allows the use of even longer columns and/or smaller particle sizes since elevated temperatures lower eluent viscosity and reduce the overall backpressure.

Amazing peak capacity The entire assembly is designed for heavy use so you can utilize the full pressure range of your UHPLC front-end (up to 1000 bar) and benefit from small particles and long columns. Longer columns and longer gradients both contribute to more identifications.

|7

RT reproducibility of peptide EAVNQVIALLDSGALR from

500 ng E.coli complex sample for 20 repeat runs in different

temperature ranges.

4,087 unique proteins were identified from a single HPLC

separation of a 1000 ng human cell digest with a

50 cm EASY-Spray column.

0 20 40 60 80 100 120 140 160 180 200 220 240 260Minutes

0

10

20

30

40

50

60

70

80

90

100

Rela

tive

Abun

danc

e

10.17414.7132

25.36431.2003 145.65

495.291462.37576.2874 122.88

472.7680112.49590.8126

182.62655.853883.78

554.285841.51415.7122

104.52513.306981.70

656.2866

238.66872.4122184.82

839.4651151.64757.3942

180.05697.3755

225.06966.5861161.02

799.8738 198.38851.4542

216.45595.3321

254.71932.7183

EASY-Spray source series is fully compatible with the following Thermo Scientific instrument families:

Thermo Scientific™ Dionex™ UltiMate™ 3000 RSLCnano

for versatile and flexible chromatography.

EASY-Spray Source Order No. ES081

EASY-Spray NG Source Order No. ES082

Thermo Scientific™ EASY-nLC™ 1000 LC For effortless sample introduction.

Full compatibility

Thermo Scientific™ Orbitrap Elite™ mass spectrometer The ultra-high resolution capability of the Orbitrap Elite mass spectrometer creates new possibilities in research and discovery.

Thermo Scientific™ Q Exactive™ mass spectrometerThe benchtop Q Exactive mass spectrom-eter is a powerful HR/AM instrument to identify, quantify, and confirm.

Thermo Scientific™ Velos Pro™ mass spectrometerThe Velos Pro is the pinnacle of ion trap mass spectrometry that delivers enhanced performance for complexsample analyses.

Thermo Scientific™ Orbitrap Fusion™ Tribrid™ mass spectrometerThree mass analyzers workingtogether to produce unmatched analytical results.

Thermo Scientific™ TSQ Quantiva™ and TSQ Endura™ mass spectrometersdeliver the highest sensitivity with lowest noise for the quantitative analysis of small molecules, peptides, biosimilars and biologics.

Thermo Fisher Scientific, Sunnyvale, CA USA is ISO 9001:2008 Certified.

Australia +61 3 9757 4486Austria +43 1 333 50 34 0Belgium +32 53 73 42 41Brazil +55 11 3731 5140China +852 2428 3282

Denmark +45 70 23 62 60France +33 1 60 92 48 00Germany +49 6126 991 0India +91 22 6742 9494Italy +39 02 51 62 1267

Japan +81 6 6885 1213Korea +82 2 3420 8600Netherlands +31 76 579 55 55Singapore +65 6289 1190 Sweden +46 8 473 3380

Switzerland +41 62 205 9966Taiwan +886 2 8751 6655UK/Ireland +44 1442 233555USA and Canada +847 295 7500

www.thermoscientific.com/easyspray©2012 Thermo Fisher Scientific Inc. All rights reserved. ISO is a trademark of the International Standards Organization. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details.

BR70044_E 06/12S