The use of Artemia nauplii as live food for the rearing of fish and crustacean larval stages, has been one of the most important steps in the development of marine aquaculture.

Advantages

*Different size*They are visible as prey and are highly palatable to the larvae*With special feeds can be given to improve its nutritional value

*Their carapace are digested easily with digestive secretion

Disadvantages

* If nauplii and shell are not separated well, larvae take them together

*Nauplii consume their yolk in a short time

Advantages*Cysts can be stored for many years and live nauplii can be obtained after 24 to 36 h incubation.

*Incubation is easy

*They tolerate wide range of temperatures and salinities

*applicability of disinfection

*Because of wide range of size, it can be used for feeding different species

Disadvantages

*Even if artemia cysts still remain an expensive product

*Their market availability shows fluctuations

*Sources of artemia cyct are threatened day by day

HATCHERY OWNERS

• A. salina Linnaeus 1 7 58: England (extinct ), Mediterranean;• A. tunisiana Bowen and Sterling 1978 synonym of A. salina;• A. parthenogenetica Barigozzi 1974, Bowen and Sterling 1978: Europe, Africa, Asia and Australia • A. urmiana Gunther 1990: Iran• A. sinica Yaneng 1989: Middle and East Asia• A. persimilis Piccinelli and Prosdocimi 1968: Argentina;• A. franciscana America, Carribean and Pacifik Island,

A.(franciscana) franciscana and A.(franciscana) monica (Mono Lake, California);• Artemia sp. Pilla and Beardmore 1994: Kazakhstan

Phylum: Arthropoda

Classis: Crustacea

Subclassis: Branchiopoda

Genus: Artemia

Species: Artemia sp

Morphology Adult Artemia (10-15 mm in length) have an elongated body with two stalked complex eyes, a linear digestive tract, sensorial antennules. The male has a paired reproductive organ in the posterior part of the trunk region. Female Artemia can easily be recognized by the brood pouch or uterus. Eggs develop in two tubular ovaries in the abdomen. Once ripe they become spherical and migrate via two oviducts into the unpaired uterus.

Ecology

*Artemia populations are found in about 500 natural salt lakes

scattered throughout the tropical, subtropical and temperate

climatic zones, along coastlines as well as inland.

*eurythermal and euryhaline

1 %0 – 250 %0

30 %0 - 35 %0 optimal

6 – 35° C

25-30° C incubation of cysts

*Its physiological adaptations to high salinity provide

a very efficient ecological defense against predation

*As Artemia is incapable of active dispersion,

wind and waterfowl (especially flamingos) are the

most important natural dispersion vectors

Naupli

Metanaupli

Adult

Partenogenetik

Ovovivipar

REPRODUCTIONParthenogenetic and bisexual Artemia strains exist, where ovoviviparous and oviparous reproduction alternates. Cysts hatching gives ovoviviparous offspring; eggs are retained inside the uterus until embryonic development is fully completed.

In extreme conditions the embryos only develop up to the gastrula stage. At this moment they get surrounded by a thick shell, enter a state of metabolic standstill or dormancy (diapause) egg (200-300 µm) and are then released by the female (oviparous)

Cyst

Feeding

As a non-selective filter feeding, brine shrimp feed

on particulate matter of biological origin, bacteria

and algae of

suitable size (1-50 µm ).

Cyst source Length (µm) Dry weight (µg) Energy content (10-3 Joule)

San Francisco San Francisco Bay, CA USABay, CA USA

428428 1.631.63 366366

Macau, BrasilMacau, Brasil 447447 1.741.74 392392

Great Salt Lake, Great Salt Lake, UT-USAUT-USA

486486 2.422.42 541541

Shark Bay, Shark Bay, AustraliaAustralia

458458 2.472.47 576576

Chaplin Lake, Chaplin Lake, CanadaCanada

475475 2.042.04 448448

Tanggu, Bohai Tanggu, Bohai Bay, ChinaBay, China

515515 3.093.09 681681

Aibi Lake, ChinaAibi Lake, China 515515 4.554.55 --

Yuncheng, ChinaYuncheng, China 460460 2.032.03 --

Lake Urmiah, IranLake Urmiah, Iran 497497 -- --

Size, individual dry weight and energy content of Artemia instar I nauplii from different cyst sources hatched in standard conditions (35 g.l-1,25°C)

AF Nauplii length: ± 430 µm

High amount HUFA: > 15 mg/g dwtNo need extra enrichment

AF 480Nauplii length : ± 480 µm

High amount HUFA: > 15 mg/g dwtNo need extra enrichment

10 $ 30 g 8$ 30 g

Original Artemia-Eier in PLATINUM-QUALITÄT! Schlupfrate: 95% - Schlupfzeit: 9-18 Stunden – Cyst count/gram: 330.000 – Protein: 61,8% - Carbohydrate: 21,5% - Ash: 5,5% - Min. 8,0% area, 20:5 omega-3 - Min. 3,5% area, 22:6 omega-3 - Min. 3,0% area, 18:3 omega-3. Inhalt: 10 gramm original ARGENTEMIA PLATINUM EGGS!

10€ / 100g

Incubation of cysts

*25 °C

*%0 30-35 salinity

*2000 lux continuous

*oxygen 2-4 mg/l

*pH 8-9

*strong aeration

*cylindrical shape tank

Decapsulation

*Hydration of egg

*Addition Calcium hypochlorite or Sodium hypochlorite

*Addition NaOH or NaNO3

*Change egg colour

*Washing egg

*Addition Sodium thiosulfate

*Move to incubation tank

For decapsulation process;

Ca(OCl)2 Calcium hypochlorite

NaOCl Sodium hypochlorite

KOCl Potassium hypochlorite

* 0.5 g active chlorine must be per 1 g egg

* It can be used NaOH for supplying pH value higher than 10

* For 1 g egg

10 ml NaOCl,

% 40, 0.33ml NaOH

3.67 ml marine water

Total 14 ml solution

After Decapsulation, washing tap water and adding % 1

sodium thiosulfate (Na2S2O3)

Advantages decapsulation ;

•Nauplii that are hatched out of decapsulated cysts have a higher energy content

•Disinfection of egg

•Nauplii size near each other and smaller (5 %) than without decapsulation

•Increase of hatching rate

•For decapsulated cysts, illumination requirements for hatching would be lower

Disadvantages decapsulation

* When the temperature reach to 40°C, embryo or egg may damage

Egg 200-300 µm

A0 350-500 µm

A1 700-800 µm

A2 800-900 µm

Factors of affecting hatching rates;- Physicochemical factors

*Ion composition

*Temperature

*Salinity

*pH

*amount of oxygen

*Illumination

*Water quality

-Artemia

*Strain/Origin

*Density

Enrichment of Artemia

*Some stocks of Artemia nauplii have shown a deficiency in EPA

(20:5n-3) and DHA (22:6n-3)

*Nauplii are harvested, washed, counted and moved to enrichment

tank

*Prepared enrichment solution according to enrichment time and

kind

*During enrichment operation, oxygen level has to be higher than

3-4 mg/l

*Larger quantities of enriched nauplii can be produced but require

storage at 5C at a density of 5 000/ml with appropriate aeration

Dolphus - cuve

0 10 20 30 40

Days

Rotifer Rotifer (8-12 ind/ml)(8-12 ind/ml)

Algae Algae (20-40 x 10(20-40 x 1044 cell/ml ) cell/ml )

ArtemiaArtemia nauplii nauplii (0.5-2 ind/ml)(0.5-2 ind/ml)

ArtemiaArtemia metanauplii metanauplii (0.5-2 ind/ml)(0.5-2 ind/ml)

Microdiet Microdiet (2-10% biomass)(2-10% biomass)

Larval feeding of gilthead sea bream

References

Akbary, P., Hosseini, S. A. and Imanpoor, M. 2011. Enrichment of Artemia nauplii with

essential fatty acids and vitamin C: effect of rainbow trout (Oncorhynchus mykiss) larvae

performance. Iranian Journal of Fisheries Sciences, 10(4):557-569.

Conceiçao, L. E., Yufera, M., Makridis, P., Morais, S., Dinis, M.T. 2010. Live Feeds For Early

Stages of Fish Rearing. Aquaculrure Research, 41, 613-640.

Lavens, P., Sorgeloos, P. 1996. Manual on the production and use of live food for

aquaculture. FAO Fisheries Technical Paper, 361.

Lakshmanasenthil, S. Vinothkumar,T., D. Geetharamani, D., Maruthupandi, T. 2013. Influence

of micro algae in enrichment of Artemia salina for aquaculture feed enhancement. J. Algal

Biomass Utln. 2013, 4 (2): 67–73

Pechmanee, T. 1989. Food Organisms For Seabass Larval Rearing. Report on the Training

Course on Seabass Breeding and Culture. Project reports (not in a Series) No.9 FAO.

Schwarz, M., Delbos, B., Mclean, E., Craig, S. 2009. Intensive Marine Finfish Larviculture.

Virginia Cooperative Extension. Publication 600-050.

Tamaru,C.S., Ako, H., Paguirigan, R. Pang, L. Enrichment of Artemia For Use in Freshwater

Ornamental Fish Production. Center for Tropical and Subtropical Aquaculture Publication

Number 133

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