The Sargasso Sea The Sargasso Sea “Metagenome”“Metagenome”

R/V Weatherbird II

The Sargasso Sea The Sargasso Sea “Metagenome”“Metagenome”

Slide courtesy of KB Heidelberg

Sequence reads 1,662,328

sequence (Mbp) 1,361

Contigs 121,477

sequence (Mbp) 256.0

Scaffolds 64,398

span (Mbp) 400.0

Mini-scaffolds 217,015

span (Mbp) 820.7

sequence (Mbp) 353.3

Singletons 215,038

sequence (Mbp) 169.9

Total nonredundant sequence (Mbp) 779.2

Scaffolds>3x 333

Span>3x (Mbp) 30.9

Annotated genes 1,001,987

16S rRNA genes 1,164

Frequency and diversity of Frequency and diversity of RhodopsinsRhodopsins

How common are these How common are these genes, was it an isolated genes, was it an isolated occurrence or are they occurrence or are they globally distributed?globally distributed?

Are rhodopsins only found Are rhodopsins only found in the SAR86 group (in the SAR86 group (--Proteobacteria) or are Proteobacteria) or are they found in diverse they found in diverse groups?groups?

Proteorhodopsin genes Proteorhodopsin genes are distributed among are distributed among divergent marine divergent marine bacterial taxa.bacterial taxa.de la Torre et al 2003. PNAS. 100:12830-12835

Venter et al. 2004. Science. 304:66-74

The previous data The previous data from Monterey Bay from Monterey Bay still only suggested still only suggested the rhodopsins were the rhodopsins were in the Proteobacteria.in the Proteobacteria.

Sargasso Sea data set Sargasso Sea data set allowed the allowed the expansion of the taxa expansion of the taxa that contain that contain rhodopsinsrhodopsins

The previous data The previous data from Monterey Bay from Monterey Bay still only suggested still only suggested the rhodopsins were the rhodopsins were in the Proteobacteria.in the Proteobacteria.

Sargasso Sea data set Sargasso Sea data set allowed the allowed the expansion of the taxa expansion of the taxa that contain that contain rhodopsinsrhodopsins

CFB

Sorcerer II Global Ocean Sampling Expedition