The ProteomeWorks System is the global alliance of Micromass and Bio-Rad Laboratories dedicated to furthering proteomics research.

The ProteomeWorks System~ New Mass Spectrometry Systems ~

At Pittcon 2001 Micromass introduced a range of e-MSTM enabled MALDI-TOF MS / MS-MS & ESI LC-MS-MS systems and Mass-InformaticsTM Software to leverage The ProteomeWorksTM System

• M@LDI HT TM

• Q-Tof UltimaTM

• Q-Tof microTM

Micromass’ new networked MALDI TOF-MS & sample management system

“2D-gel-MS analyser for proteomics factories”

M@LDI HT TM [new instrument]

M@LDI HT TM - Networked MALDI-TOF MS

REFLECTRON Peptide Mass Fingerprinting

LINEAR Intact Proteins

ELECTRO MAGNETIC GRIPPER

TARGET PLATE CASSETTE 2

X - Y ROBOTIC ARM

TARGET PLATE CASSETTE 1

HORIZONTAL AXIS MOTOR

VERTICAL AXIS MOTOR

BAR CODE SCANNERVACUUM LOCK LID

IN COLUMNOUT COLUMN

VERTICAL FLIGHT TUBE AND REFLECTRON HOUSING

M@LDI HT TM - Sample Management System 80 x 96/384-Sample Targets (bar coded)

M@LDI HT TM - Networked MALDI-TOF MS

• Linear and reflectron ion optics options• 80 x 96/384-Sample target management system• Bar coded sample target plates• Automated Laser Positioning System (ALPS) for

sample target alignment and sample indexing• Intelligent acquisition / calibration algorithm• Web-enabled Client-Server Mass-Informatics for

interrogation of non-redundant protein dB

…converts samples into knowledge not ‘just’ spectra

ProteinLynxTM Global SERVER

web-enabled Mass-informatics

ProteinLynxTM Global SERVER export to PDQuest Annotation Tools

PDQuest Annotation Tools

C L I C K

MS & MS-MS Techniques for Peptide / Protein Analysis

Technique Data Type Dynamic

Range

Specificity Speed Data InterpretationConstraints

MS MALDI MH + Low Low High Known Protein dB

MALDI PSD MH + Low Low Medium Known Protein dB

MS-MSMALDI MH +

Fragments

Low Medium High Known Protein dB

Known nucleotide dB

ESI + HPLC MH 2 ++ &

Fragments

High High Medium Known Protein dB

Known nucleotide dB

*ESI + HPLC(de novo)

Amino Acid

Sequence

High High Medium Homologues (BLAST)

Time (PCR)

Q-Tof UltimaTM

family

[new instruments]

DDATM

Micromass’ SuperPremium family of MALDI MS-MS and ESI MS-MS systems for PROTEOMICS and structural analysis

DDATM

Q-Tof Ultima…the SuperPremium family of MS-MS

• Q-Tof Ultima MALDI• The high throughput MALDI MS-MS

• Q-Tof Ultima API• The SuperPremium API LC-MS-MS

• Q-Tof Ultima GLOBAL• The flexible research platform for API LC-MS-MS / MALDI MS-MS

•

Q-Tof UltimaTM MALDI [new instrument]

• Dedicated MALDI MS-MS system• V & W-OPTICS options • ProteinLynx Global SERVER• e-MS enabled

Q-Tof Ultima MALDI with VOPTICS

10,000 FWHM

Q-Tof Ultima MALDI with WOPTICS

20,000 FWHM

MALDI MS-MS is superior to PSD

ADH 1 pm/µl PSD

ADH 1 pm/µl Q-Tof Ultima MALDI MS-MS

ProteinLynxTM Global SERVER ...Matching MS-MS spectra to dB of known protein or nucleotide sequence.

Q-Tof UltimaTM API [new instrument]• Dedicated API (ESI) LC-MS-MS system• V & W-OPTICS options • DDA - Precursor Ion Discovery (patent applied for)

– “Neutral Loss”– “Precursor Ion”– High resolution– Exact mass– Compatible with HPLC

• ProteinLynx Global SERVER• e-MS enabled

Q-Tof Ultima API with WOPTICS

20,000 FWHM

Q-Tof UltimaTM API ...comparison of V & W-OPTICS Resolution

The superior peptide sequence coverage provided by ESI MS-MS data enables de novo sequencing

Sequencing results from MS-MS of m/z 841.5

Q-Tof UltimaTM GLOBAL [new instrument]• Flexible API & MALDI MS-MS research platform• API / MALDI changeover without venting• V & W-OPTICS options • LC-DDA-MS

– Precursor ion discovery– “Neutral Loss”– “Precursor Ion”– Exact mass

• ProteinLynx Global SERVER• e-MS enabled

Q-Tof Ultima GLOBAL

Q-Tof microTM

[new instrument]

Micromass’ finest API Q-Tof technology for

structural analysis & protein characterisation…

• SMALLER• SMARTER• WIRED

DDATM

Q-Tof microTM

…SMALLER• ZSPRAYTM nanoflow ESI inlet technology• 5000 FWHM resolution• 5ppm RMS exact mass measurement• Dynamic External Calibration (DXCTM)• 20000 m/z range

Q-Tof microTM

…SMARTER …WIRED• Data Directed Analysis (DDA)

– Automates analysis of protein digests by LC-DDA-MS• Precursor Ion Discovery (patent applied for)

– “Neutral Loss”– “Precursor Ion”– High resolution– Exact mass

• Compatible with HPLC• Maximising data quality

Data Directed Analysis DDATM

Exclusion of 1+

background ions

The New e-MSTM Enabled Mass Analysers of The ProteomeWorksTM System...

msinnovation.com

The ProteomeWorks System is the global alliance of Micromass and Bio-Rad Laboratories dedicated to furthering proteomics research.