The observed and theoretical peptide sequence information Cal.MassObserved. Mass ±da±ppmStart...
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The observed and theoretical peptide sequence information Cal.Mass Observed. Mass ±da ±ppm Start Sequence End Sequence Ion Score C.I% modification 945.5403 945.5346 -0.0057 -6 144 151 FLPVNEK 1302.5896 1302.5812 -0.0084 -6 159 169 SHPDPPCHEVK Carbamidomethy l (C)[7] 1612.8224 1612.8035 -0.0189 -12 351 366 CVNAAINTAAGVAPQR 111 100 Carbamidomethy l (C)[1] 1634.8458 1634.8251 -0.0207 -13 367 380 SESPLTYIILN KPC Carbamidomethy 1914.9127 1914.8998 -0.0129 -7 159 174 SHPDPPCHEVKNEQLK 66 99.997 Carbamidomethy l (C)[7] 2234.0222 2233.9993 -0.0229 -10 179 197 TEHSESHHAPIVEEHHETK 118 100 2628.2073 2628.1943 -0.013 -5 238 260 HSEEAHHEVVAEAPHEEHSEP LK 153 100 2674.2129 2674.2361 0.0232 9 286 308 HEALHDAPAHIEHVEETSHEE EK 2818.2034 2818.2129 0.0095 3 261 285 ELSTESHESSSEEHSAEEHHV EPSK 3449.6018 3449.5957 -0.0061 -2 98 128 SSVPVVVTNKEDAAGYEYSYL VYDENTGDHK Prote in name Accessio n No. Protein Score C.I.% Total ions score MW PI Amino acid length Best scor e peptide s Intensi ty Protein domain Cuticl e Protei n BGIBMGA0 00274 597 100 448 41.89 4.89 380 153 10 60.8 Cuticle protein RR- 2 motif 90 Table S2.The protein and Peptides information of BmASSCP1 identified by MALDI-TOF MS were listed below. Spot 1 excised from 2-D map of ASG was identified as a putative RR-2 cuticle protein.
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Peptide summary report Mascot Score Histogram Ions score is -10*Log(P), where P is the probability that the observed match is a random event. Individual ions scores > 53 indicate identity or extensive homology (p
Transcript of The observed and theoretical peptide sequence information Cal.MassObserved. Mass ±da±ppmStart...
The observed and theoretical peptide sequence information Cal.Mass Observed.
Mass±da ±ppm Start
SequenceEnd Sequence Ion
ScoreC.I% modification
945.5403 945.5346 -0.0057 -6 144 151 FLPVNEK
1302.5896 1302.5812 -0.0084 -6 159 169 SHPDPPCHEVK Carbamidomethyl (C)[7]
1612.8224 1612.8035 -0.0189 -12 351 366 CVNAAINTAAGVAPQR 111 100 Carbamidomethyl (C)[1]
1634.8458 1634.8251 -0.0207 -13 367 380 SESPLTYIILN KPC
Carbamidomethy
1914.9127 1914.8998 -0.0129 -7 159 174 SHPDPPCHEVKNEQLK 66 99.997 Carbamidomethyl (C)[7]
2234.0222 2233.9993 -0.0229 -10 179 197 TEHSESHHAPIVEEHHETK 118 100
2628.2073 2628.1943 -0.013 -5 238 260 HSEEAHHEVVAEAPHEEHSEPLK
153 100
2674.2129 2674.2361 0.0232 9 286 308 HEALHDAPAHIEHVEETSHEEEK
2818.2034 2818.2129 0.0095 3 261 285 ELSTESHESSSEEHSAEEHHVEPSK
3449.6018 3449.5957 -0.0061 -2 98 128 SSVPVVVTNKEDAAGYEYSYLVYDENTGDHK
Protein name
Accession No.
Protein Score
C.I.% Total ions score
MW PI Amino acid length
Best score
peptides Intensity Protein domain
Cuticle Protein
BGIBMGA000274
597 100 448 41.89 4.89 380 153 10 60.8 Cuticle protein RR-2 motif 90
Table S2.The protein and Peptides information of BmASSCP1 identified by MALDI-TOF MS were listed below. Spot 1 excised from 2-D map of ASG was identified as a putative RR-2 cuticle protein.
Monoisotopic mass of neutral peptide Mr(calc): 1611.816724 from(1612.824000,1+) intensity(0.0000) index(2)
MS/MS Fragmentation of CVNAAINTAAGVAPQRFound in gi|290560913 in NCBInr, cuticular protein RR-2 motif 90 [Bombyx mori]
Matches : 80/298 fragment ions using 74 most intense peaksNCBI BLAST search of CVNAAINTAAGVAPQR(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Figure S3.MS/MS spectra of single peptide identification of BmASSCP1.
Peptide summary report Mascot Score HistogramIons score is -10*Log(P), where P is the probability that the observed match is a random event.Individual ions scores > 53 indicate identity or extensive homology (p<0.05).Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.
Query Observed Mr(expt) Mr(calc) Delta Miss Score Expect Rank Unique Peptide
6 1612.8240 1611.8167 1611.8151 0.0016 0 119 1.7e-08 1 U K.CVNAAINTAAGVAPQR.S
Search ParametersType of search : MS/MS Ion Search Enzyme:TrypsinFixed modifications : Carbamidomethyl (C) Variable modifications : Oxidation (M)Mass values : Monoisotopic Protein Mass: UnrestrictedPeptide Mass Tolerance : ± 0.15 Da Fragment Mass Tolerance: ± 0.25 DaMax Missed Cleavages : 1 Instrument type : MALDI-TOF-TOFNumber of queries : 12
Figure S4. PMF of peptides from trypsin digests of BmASSCP2.A total of 20 monoisotopic peptide masses were submitted to the database searching under the conditions described in methods, and 14 peptides matched the putative cuticle of silkworm, with sequence coverage of 50.3%.The Mass and PI were 31.7 KD and 8.88 ,respectively.
Measured Computed AM/OV Diff 0/00 Res Sequence
1004.800 1004.154 A/0 -0.65 -0.64 218-227 R YAAPSVVAAR Y
1023.747 1023.156 A/0 -0.59 -0.58 173-181 R YEAAPFVAR Y
1406.731 1406.557 A/0 -0.17 -0.12 40- 50 R IEEFDPLPQYR F
1453.816 1453.704 A/0 -0.11 -0.08 203-217 R YATAPVAPAPVVAAR Y
1670.724 1670.885 A/0 0.16 0.10 157-172 R YAVAPAYSAPYVTAAR Y
1809.717 1809.996 A/0 0.28 0.15 90-106 R VVDYAADSINGFNAVVR K
1811.676 1809.996 A/0 -1.68 -0.93 90-106 R VVDYAADSINGFNAVVR K
1907.665 1907.023 A/0 -0.64 -0.34 71- 88 R NGDLVQGSYSLVEPDGTR R
1926.903 1927.274 A/0 0.37 0.19 108-127 K EPLVAAAPAVVAEPAVVPAR Y
1937.807 1938.170 A/1 0.36 0.19 90-107 R VVDYAADSINGFNAVVRK E
1965.793 1966.183 A/1 0.39 0.20 89-106 R RVVDYAADSINGFNAVVR K
1981.787 1982.312 A/0 0.52 0.26 182-202 R YAAAPVAAAPVAAAPAPIYAR Y
2054.875 2055.448 A/1 0.57 0.28 107-127 R KEPLVAAAPAVVAEPAVVPAR Y
2307.454 2308.399 A/1 0.94 0.41 51- 70 R FGYDVADSLTGDYKSQQEER N
Protein name Accession No. Protein Score
Coverage MW PI Protein Domain Amino acids length
Cuticle Protein BGIBMGA010231 186 50.3% 32 8.88 Chitin_ bind 4 316
Table S3.The protein and Peptides report of BmASSCP2 identified by MALDI-TOF MS
