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THE NUCLEIC ACIDS€¦ · DNA IS MADE OF TWO STRANDS OF POLYNUCLEOTIDE •The sister strands of the...
Transcript of THE NUCLEIC ACIDS€¦ · DNA IS MADE OF TWO STRANDS OF POLYNUCLEOTIDE •The sister strands of the...
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THE NUCLEIC ACIDS
© 2016 Paul Billiet ODWS
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Friedrich Miescher in 1869
• Isolated what he called
nuclein from the nuclei of
pus cells
•Nuclein was shown to
have acidic properties,
hence it became called
nucleic acid
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Two types of nucleic acid are found
•Deoxyribonucleic acid (DNA)
•Ribonucleic acid (RNA)
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The distribution of nucleic acids in the
eukaryotic cell
• DNA is found in the nucleus
with small amounts in mitochondria and
chloroplasts
• RNA is found throughout the cell
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DNA as genetic material: The
circumstantial evidence1. Present in all cells and virtually restricted to the
nucleus
2. The amount of DNA in somatic cells (body cells) of any
given species is constant (like the number of
chromosomes)
3. The DNA content of gametes (sex cells) is half that of
somatic cells.
In cases of polyploidy (multiple sets of chromosomes)
the DNA content increases by a proportional factor
4. The mutagenic effect of UV light peaks at 253.7nm.
The peak for the absorption of UV light by DNA
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NUCLEIC ACID STRUCTURE
•Nucleic acids are
polynucleotides
•Their building blocks are
nucleotides
© 2016 Paul Billiet ODWS
P
P
P
P
P
P
G
C
C
A
T
T
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NUCLEOTIDE STRUCTURE
PHOSPHATE
SUGAR
Ribose or
Deoxyribose
BASE
PURINES PYRIMIDINES
Adenine (A)
Guanine(G)
Cytocine (C)
Thymine (T)
Uracil (U)
P
P
P
P
P
P
G
C
C
A
T
T
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Types of nucleotides
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Deoxyribose or Ribose is a pentose
C1
C5
C4
C3 C2
O
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P
P
P
P
P
G
C
C
A
T
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RIBOSE DEOXYRIBOSE
CH2OH
H
OH
C
C
OH OH
C
O
H HH
C
CH2OH
H
OH
C
C
OH H
C
O
H HH
C
Spot the difference
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THE SUGAR-PHOSPHATE
BACKBONE
•The nucleotides are all
orientated in the same
direction
•The phosphate group joins
the 3rd Carbon of one sugar
to the 5th Carbon of the next
in line.
P
P
P
P
P
P
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ADDING IN THE BASES
•The bases are attached to
the 1st Carbon
•Their order is important
It determines the genetic
information of the molecule
P
P
P
P
P
P
G
C
C
A
T
T© 2016 Paul Billiet ODWS
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DNA IS MADE OF
TWO STRANDS OF
POLYNUCLEOTIDE
P
P
P
P
P
P
C
G
G
T
A
A
P
P
P
P
P
P
G
C
C
A
T
T
Hydrogen bonds
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DNA IS MADE OF TWO STRANDS OF
POLYNUCLEOTIDE
• The sister strands of the DNA molecule run in opposite directions (antiparallel)
• They are joined by the bases
• Each base is paired with a specific partner:
A is always paired with T
G is always paired with C
Purine with Pyrimidine
• Thus the sister strands are complementarybut not identical
• The bases are joined by hydrogen bonds, individually weak but collectively strong.
© 2016 Paul Billiet ODWS
C
G
G
T
A
A
P
P
P
P
P
P
G
C
C
A
T
T
Hydrogen bonds
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Purines & Pyrimidines
Adenine
CytosineGuanine
Thymine
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Structure of nucleotidesP
P
P
P
P
P
G
C
C
A
T
T
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Nucleotide Structure - 4Base-Sugar-PO4
2-
N
N
5
61
2
3
4
OC
5’
1’4’
3’ 2’
OH
Nucleoside
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Nucleotide Structure - 4Base-Sugar-PO4
2-
P
O
O
O
O
N
N
5
61
2
3
4
OC
5’
1’4’
3’ 2’
OH
Nucleoside Monophosphate
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+ HPO4 2-O N
N
N
N
NH2
HO OH
OPO
O
O
P
O
O
O
PO
O
O
ATP
H2O O N
N
N
N
NH2
HO OH
OPO
O
O
P
O
O
O
ADP
+ ~31 KJ/mol (7.4 Kcal/mol)
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20
sugar base
sugar base
phosphate
sugar base
phosphate
sugar base
phosphate
sugar base
phosphate
nucleoside nucleotides
nucleic acids
Nucleoside, nucleotides and nucleic acids
The chemical linkage between monomer units in nucleic acids
is a phosphodiester
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Deoxyribonucleotides of nucleic acids
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PP
(PPi)
Nucleic Acid StructurePolymerization
P P P
S
N
C
P P P
S
N
C
+
P P P
S
N
C
P
S
N
C
Phosphodiesterase
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Formation of polynucleotide
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Nucleic Acid StructureThe double helix
Major
Groove
Minor
Groove
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Physical Structure (cont’d)
Chains are anti-parallel (i.e in opposite directions)
Diameter and periodicity are consistent 2.0 nm
10 bases/ turn
3.4 nm/ turn
Width consistent because of pyrimidine/purine pairing
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Wilkins & Franklin (1952): X-ray crystallography
kentsimmons.uwinnipeg.ca/cm1504/Image265.gif© 2016 Paul Billiet ODWS
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Erwin Chargaff’s Data (1950-51)
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Watson & Crick Base pairing
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The Double Helix (1953)
www.chem.ucsb.edu/.../images/WatsonCrick.jpg© 2016 Paul Billiet ODWS
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