The Mouse Bioinformatics Research Network (MBIRN) Cal Tech- Russ Jacobs Duke- G. Allan Johnson Mike...
Transcript of The Mouse Bioinformatics Research Network (MBIRN) Cal Tech- Russ Jacobs Duke- G. Allan Johnson Mike...
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The Mouse Bioinformatics Research Network (MBIRN)
Cal Tech- Russ JacobsDuke- G. Allan Johnson
Mike FehnelUCLA- Art Toga
UCSD- Mark EllismanMaryann Martone
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Goals
The unique challenges of MBIRN
Representative collaborations
The value of connections
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MBIRN- a heterogeneous data collectionImages
MR,optical,EMRegulatory
IACUCC, FundingAgencyProject Description
Goals,Literature,DesignSpecimen
Age,Weight,Sex,GenotypeExperimental
TR/TE,Resolution, Special StainsAnalysis
Segmentation,volumes,visualizationBehavioral Data (future)
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CollaborationsShiverer
UCLA,Cal Tech, UCSD,DukeAlpha-synuclein overexpressor
UCSD,DukeDAT/KO, Rheeler
MBIRN- MorphBIRNAstrocytoma
UCSD UNC Duke
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Alpha-SYN aggregates in brains of tg mice
Diana PriceNatalie MacLean
~0.5 TB
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Alpha-synuclein MouseDiana Price, UCSD
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Actual Diffusion Actual Diffusion RestrictionRestriction
Tensor ModelTensor Model
Diffusion Tensor Model of Restricted, Anisotropic Diffusion
Caltech 2004 Tyszka & Jacobs
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3D DTI Shiverer MouseCNS +/+
CNS shi/shi
• Naturally occurring mutant- a model of demyelating disease
• Myelin basic protein (MBP) gene deletion.
• Virtual absence of compacted myelin in CNS, but not PNS.
• Motor tremors, seizures and significantly reduced lifespan (approx 90 days).
Caltech 2004 Tyszka & Jacobs
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0.0
1.0
FA
[D]
Mean Fractional Anisotropy (Control)
Caltech 2004 Tyszka & Jacobs
3D Diffusion Tensor7 sets @ 512x256x256
1.75 Gb
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0.0
1.0
FA
[D]
Mean Fractional Anisotropy (Shiverer)
Caltech 2004 Tyszka & Jacobs
3D Diffusion Tensor
7 sets @ 512x256x256
1.75 Gb
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Summary
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Image Registration
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Brain Morphology in DAT-KO MiceAki Laakso, Ph.D., Duke University
3D reconstruction of caudate-putamenand ventriclesQuickTime™ and a
Video decompressorare needed to see this picture.
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P<0.001 n.s.
Two-way repeated measures ANOVA
Profile of caudate-putamen in DAT-KO mice:
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Stereological cell counts
Same brains that were used for MRI
Striatum divided into anterior and posterior parts, based on MRH profile analysis
All nuclei: Neuronal nuclei:
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Brain Morphology in DAT-KO MiceMichel Cyr, Ph.D., Duke University
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Reeler Mouse MBIRN-MorphBIRNWilliam Wetsel, Duke
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Connections
Man Mouse CelllarSubcellular
MBIRN
Morph BIRN
fMRI BIRN
NCRR Resources
International Research Community
Tools Data Ideas People
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QuickTime™ and aDV/DVCPRO - NTSC decompressor
are needed to see this picture.