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The emerging technology in the field of forensics......
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Transcript of The emerging technology in the field of forensics......
NANO-FORENSIC ANALYSIS TO IDENTIFY,
INDIVIDUALIZE, AND EVALUATE EVIDENCE
USING NANOPHASE MATERIALS
AIM
• To prepare Fe2O3 nanoparticles by using simple synthesis
process.
• To study the effectiveness of this synthesis process in
application field.
• To characterize the sample using XRD and UV technique.
INTRODUCTION-Iron oxide
• Iron oxides are chemical compounds composed of iron
and oxygen.
• Iron oxides are widely used as inexpensive, durable
pigments in paints, coatings and colored concretes.
• Colors commonly available are in the “earthy” end of the
yellow/orange/red/brown/black range.
SYNTHESIS METHOD
SYNTHESIS METHOD
• Prepared sodium borohydride solution was added drop wise into prepared
ferrous chloride solution until the black precipitate is obtained.
• Iron nanoparticles gets attracted to the magnetic stirrer and centrifuged to
get the extract.
• And repeatedly washed with acetone to bring the pH to 7.
centrifuge
• Bi-phase separation
• Centrifuged repeatedly with acetone to bring the pH to 7.
• At 2500 rpm.
heating
• Heated to remove moisture completely.
• Heated at 90˚C
• Duration 15-20 min.
extract
Extracted ferrous oxide
nanoparticles
X-ray Diffraction (XRD)
• XRD is a rapid analytical technique primarily used for phase
identification of a crystalline material and can provide
information on unit cell dimensions.
• XRD is a versatile and non-destructive technique.
Bragg's Law: 2dsinθ = nλ
CHARACTERIZATION TECHNIQUE USED
Ultraviolet–visiblespectroscopy
It refers to absorption spectroscopy in the ultraviolet-
visible spectral region.
The absorption visible range directly affects the
perceived color of the
chemicals involved. In this region of the electromagnetic
spectrum, molecules undergo electronic transitions.This technique is complementary to fluorescence
spectroscopyIt is used in analytical chemistry for
the quantitative determination of different analyses, such
as transition metal ions, highly conjugated organic
compounds, and biological macromolecules.
Spectroscopic analysis is commonly carried out in
solutions but solids and gases may also be studied.
reSULTS
ReSULT
Observed
value
Standard
value
Peak
position(θ)
2ɵ structure Crystalline
size (Ǻ)
0.535 0.017 26.973 0.518 15.8
0.669 0.017 30.481 0.652 12.6
0.334 0.017 35.833 0.318 26.3
0.802 0.017 43.341 0.786 10.9
0.535 0.017 56.112 0.518 17.4
0.535 0.017 57.480 0.518 17.5
0.669 0.017 63.133 0.652 14.3
ReSULT
The synthesized ferrous oxide nanoparticle diameter wascalculated using Scherrer calculator.
The average particle size of the sample was found to be 16.4 nmwhich is at 46.27◦ using Scherrer’S formula.
ReSULTObserved
value(β)
Standard
ValUe(π/180)
Peak
position(θ) K λCrystalline
size (Ǻ)
0.535 0.017 26.973 0.9 1.54 x 10-10 17.08
0.669 0.017 30.481 0.9 1.54 x 10-10 14.13
0.334 0.017 35.833 0.9 1.54 x 10-10 30.05
0.802 0.017 43.341 0.9 1.54 x 10-10 13.96
0.535 0.017 56.112 0.9 1.54 x 10-10 27.31
0.535 0.017 57.480 0.9 1.54 x 10-10 28.33
0.669 0.017 63.133 0.9 1.54 x 10-10 26.96
DISCUSSION OF RESULT
Debye-Scherrer formula: D= [(K λ)/ (β*(π/180)* Cos(θ))]
The average particle size of the sample was found to be22.54 nm which is at 46.27◦ using DEBYE-Scherrer’S formula
ReSULt
From this UV-visible spectroscopy patterns analysis, we determined peak intensity, position and crystalline data.
Future work
To continue our process in the field of forensics field.
The advantage of our technique is that it can simultaneously
detect blood and saliva and can spatially locate and differentiate
these body fluid types.
conclusion
•The ferrous oxide nanoparticles was prepared
•After synthesis the pH value of sample was observed as 7.
•The crystal size of the ferrous oxide present in sample
was found be 49.7 nm respectively by using XRD.
•The particle size which was done by XRD was supported
by the XRD Scherer’s formula.
reference
1. Iron Oxides: Structure, Properties and Applications (Editors: Arturo I. Martinez
(Center for Research and Advanced Studies, National Polytechnic
InstituteatSaltillo,Mexico))(link:https://www.novapublishers.com/catalog/product_info.ph
p?products_id=35731)
2. Stabilization and functionalization of iron oxide nanoparticles for biomedical
applications” Esther Amstad,a Marcus Textora and Erik Reimhult*ab ” Nanoscale,
2011,3, 2819-2843;DOI: 10.1039/C1NR10173K
3. Synthesis of iron oxide nanoparticles of narrow size distribution on polysaccharide
templates“m nidhin, r indumathy, k j sreeram* and balachandran unni nair” Bull. Mater.
Sci., Vol. 31, No. 1, February 2008, pp. 93–96. © Indian Academy of Sciences
4. Detection and identification of body fluid stains using antibody-nanoparticle
conjugates.“Frascione N, Thorogate R, Daniel B, Jickells S”
2012 Jan 21;137(2):508-12. doi: 10.1039/c1an15200a. Epub 2011
Nov25.pmid:22117199[pubmed - indexed for medline]
reference
5. Dna "fingerprints" and their statistical analysis in human populations “A.Marie
PhillipsThe University of Melbourne Australia” (ICOTS6, 2002: Phillips)
6. Detection and identification of body fluid stains using antibody-nanoparticle
conjugates.“Nunzianda Frascione, Richard Thorogate, Barbara Daniel, Sue Jickells”
Department of Forensic Science and Drug Monitoring, King's College London, Franklin-
Wilkins Building, 150 Stamford Street, London, UK SE1 9NH. ;11/2011; 137(2):508-12.
DOI:10.1039;Source: PubMed
7. Romeika JM, Yan F (2013) Recent Advances in Forensic DNA Analysis. J Forensic Res
S12: 001. doi: 10.4172/2157-7145.S12-001
Thank yoU…………!!!