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The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb...
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![Page 1: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/1.jpg)
The Effects of Simulated Microgravity on Sensory Specific
Satiety in Rats Using the Hindlimb Unloading Model
Bio 250 and 450 classes
Presenters: Elizabeth Gowan, Casey Skinner, and Tyler Stinnett Funded by NASA’s South Carolina Space Grant Consortium Research Experience for Undergraduates Grant
![Page 2: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/2.jpg)
Observation: Astronauts get sick in space
• Cephalic Fluid Shift
• Space Adaptation Syndrome
• Dizziness
• Nausea
• Loss of appetite
• Loss of smell and taste
Decreased food intake = insufficient energy intake
![Page 3: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/3.jpg)
Why does this matter?
• Renewed interest in space exploration
• Difficulties reduce productivity
• Cost effectiveness in NASA spending
![Page 4: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/4.jpg)
How can we increase astronauts’ food consumption?
Study humans in simulated microgravity
Difficult and cost-prohibitive
Animal model of simulated microgravity?
•Yes! >800 papers published using this animal model
•None have attempted to manipulate feeding behaviors
![Page 5: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/5.jpg)
Is there an animal model of increased eating?
Sensory Specific Satiety (Ahn and Phillips, 1999)
A hungry rat fed until satiated on rat chow WILL NOT eat more rat chow
A hungry rat fed until satiated on rat chow WILL eat Froot Loops ®
![Page 6: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/6.jpg)
Purpose:
• To combine an existing model of simulated microgravity with an existing model of increased eating
![Page 7: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/7.jpg)
Hypothesis:
• Rats exhibit sensory specific satiety under conditions of simulated microgravity.
![Page 8: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/8.jpg)
Methods• 7 Male Sprague-Dawley Rats
• Fed Rat Chow ad-libitum except on experimental days
• Used NASA Hindlimb Unloading Model
•Institutional Animal Care and Use Committee Approved
![Page 9: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/9.jpg)
What is the Hindlimb Unloading Model?
• Simulates microgravity (Morey-Holton & Globus, 2002.)
– Cephalic Fluid Shift– Symptoms of Space Adaptation Syndrome
![Page 10: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/10.jpg)
2 week
recovery period
Horizontal
Week 1
body weight & food intake measured twice daily
Experimental Time Line
1 week recovery period
1 week recovery period
HU
Week 3
HU
Week 6
Horizontal
Week 8
![Page 11: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/11.jpg)
Weekly Procedures
Meal 1 - 90 minutesRat Chow
Meal 2 - 30 MinutesRat Chow or Froot Loops(same caloric densities)
- Counterbalanced
• Experiment• Returned to standard cages
Day 7
• Transferred to experimental cage• 14-18 hr food deprivation begins
Day 0-1
• Experiment
Day 2
• Hanging out in the
experimental cage!
Days 3-5
• 14-18 hr food deprivation begins
Day 6
Food deprivation
![Page 12: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/12.jpg)
How SSS was measured...• Food Intake (grams):
• Meal 1 at 60 and 90 minutes• Meal 2 at 30 minutes
Statistical Analysis• Two Way Repeated Measures ANOVA
•Student-Newman-Keuls Test *p<0.05
• Latency to Eat (minutes):• Meal 2
![Page 13: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/13.jpg)
Treatment
Horiz D2 HU D2 Horiz D7 HU D7
Cu
mu
lati
ve F
oo
d In
take
(g
ram
s)
0
1
2
3
4
5
6
7
8
~50%
*~50%
*
Meal 1 Food Intake at 60 and 90 Minutes
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Treatment
Horiz D2 HU D2 Horiz D7 HU D7
La
ten
cy
to
fe
ed
(m
in)
0
10
20
30
40 Rat chow/Rat chowRat chow/Froot loops
Latency to Eat Meal 2
**
*
*
![Page 15: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/15.jpg)
Treatment
Horiz D2 HU D2 Horiz D7 HU D7
Cu
mu
lati
ve F
oo
d In
take
(g
ram
s)
0
1
2
3
4 Rat chow/Rat chowRat chow/ Froot Loops
Meal 2 Food Intake at 30 minutes
*
*
*
**
~40%
~40%
*
![Page 16: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/16.jpg)
Conclusions:
• Rats in Hindlimb Unloading model exhibit Sensory Specific Satiety.
• Rats in Hindlimb Unloading model eat significantly less than their horizontal counterparts.
![Page 17: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/17.jpg)
Relevance:
• How does this relate to astronauts?
• Future Directions:– Promote increased energy intake
• Manipulate sensory properties• Increase caloric density
![Page 18: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/18.jpg)
Acknowledgements:
• Funded by a grant awarded to Drs. Davis and Hettes from NASA’s South Carolina Space Grant Consortium.
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The Effects of Simulated Microgravity on Sensory Specific
Satiety in Rats Using the Hindlimb Unloading Model
Bio 250 and 450 classes
Presenters: Elizabeth Gowan, Casey Skinner, and Tyler Stinnett Funded by NASA’s South Carolina Space Grant Consortium Research Experience for Undergraduates Grant
![Page 20: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/20.jpg)
![Page 21: The Effects of Simulated Microgravity on Sensory Specific Satiety in Rats Using the Hindlimb Unloading Model Bio 250 and 450 classes Presenters: Elizabeth.](https://reader035.fdocuments.us/reader035/viewer/2022062408/56649f145503460f94c29ad4/html5/thumbnails/21.jpg)
Latency to Feed Meal 1
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Overnight Food Intake