The Effect of Resveratrol on Fat Storage in C. elegans

By Chris LoBello

Obesity

http://www.cdc.gov/nccdphp/dnpa/obesity/trend/maps

Adipocytes

http://whyfiles.org/276metabolic_syndrome/index.php?g=3.txt

http://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpg

PPARg

http://www.juliantrubin.com/encyclopedia/biochemistry/ppar.html

Chung and Kurtev (2004)

http://eresveratrol.com/content/00/01/43/84/95/userimages/ppar_gamma.pdf

Fei and Liu (2004)

Wood and Sinclair (2004)

http://www.nature.com/nature/journal/v430/n7000/fig_tab/nature02789_F2.html

http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1224044&blobtype=pdf

Methodology

RT-PCR to determine if SIRT1 is activated

Quantum yield of fluorescent intensity determined using SensL photon counter (counts per nanosecond)

Nile Red staining solution (.1microgram/ml) Done up to days 6 to 8 in the protocol

Nile Red staining solution (.1microgram/ml) Done up to days 6 to 8 in the protocol

Group 4: 100 microliters of resveratrolN= N2 Wild Type Strain Culture

Group 3: 50 microliters of resveratrolN= N2 Wild Type Strain Culture

Group 2: 10 microliters of resveratrolN= N2 Wild Type Strain Culture

Group 1: 1 microliter of resveratrol N= N2 Wild Type Strain Culture

Control Group: 0 microliters of resveratrolN= N2 Wild Type Strain Culture

Statistical Analysis by ANOVA where p <.05 followed by Scheffe post hoc test

The Effect of Resveratrol on Fat Storage in C. elegans

BudgetVendor Order # Quantity Description Price Total Price

Carolina 173525 1 C. elegans culture kit $73.00 $73.00

Sigma-Aldrich N3013-100MG 1 100 MG Nile Red Staining $64.20 $64.20

Sigma-Aldrich R5010-100MG 1 100 MG Resveratrol $81.50 $81.50

SensL PCDMini0020 1 PCDMini High Performance

Photon Counting Device with

20μm sensor

Purpose

To determine the effects of varying amounts of resveratrol on the fat content of C. elegans

Hypothesis

Null Hypothesis: There will be equal amounts of fat content in cells with resveratrol supplemented and without resveratrol supplemented

Alternate Hypothesis: There will be less fat content in the cells supplemented with resveratrol, and that there will be an inverse relationship between resveratrol supplementation and fat content

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