The effect of parasite burden on faecally-excreted albumin ......(P=0.64) or tapeworm ELISA (P=0.35)...
Transcript of The effect of parasite burden on faecally-excreted albumin ......(P=0.64) or tapeworm ELISA (P=0.35)...
Establish parasite burden & faecal albumin
Administer appropriate anthelmin6c
Reassess faecal albumin
The effect of parasite burden on faecally-excreted albumin
School of Veterinary Medicine,University of Glasgow, Scotland, UK
The aim of this study was to determine if faecally excreted albumin, measured using a commercially available test kit, was related to the parasite burden of otherwise healthy horses.
Aim
• Qualitative or semi-quantitative. • Designed for non-laboratory environments. • Strip of carrier material containing dry
reagents which are activated upon application of a fluid sample.
Lateral flow immunoassay
After ‘Lateral Flow Immunoassay’ Springer Publications, Wong and Tse
The ‘Succeed’ lateral flow immunoassay contains monoclonal an6bodies targeted to equine haemoglobin and albumin.
Study outline
Materials and Methods
• 21 horses • Mixed breeds • Ages 6-26 (Mean 15yrs, standard deviation
5.89) • 6 mares, 14 geldings Previous parasite management strategy involved FWECs q3 months and annual tapeworm ELISA. FWEC and tapeworm ELISA pre-anthelmintic:
Interpreta6on of tapeworm ELISA: <0.2-‐ Zero/low infec.on intensity
0.2-‐0.7-‐ Moderate infec.on intensity >0.7-‐ High infec.on intensity
Results
Positive 81%
Negative 19%
Pre-anthelmintic faecal albumin
Positive 29%
Negative 71%
Post-anthelmintic faecal albumin
Moxidectin administered to all horses regardless of FWEC for seasonal treatment of encysted cyathstomes, + praziquantel if ELISA optical density > 0.2.
Statistical analysis
• McNemar’s tests conducted to identify significant differences in the proportion of albumin positive test results pre- and post- anthelmintic administration: • No significant relationship between FWEC (P=0.64) or tapeworm ELISA (P=0.35) and a positive faecal albumin (Mann-Whitney tests). • Recent anthelmintic treatment was associated with the likelihood of a positive faecal albumin test.
• However, faecal albumin was not associated with the actual degree of parasite burden (when measured by FWEC or tapeworm ELISA).
• Lack of statistical power to identify association between FWEC or tapeworm ELISA and faecal albumin.
• Now being repeated on a larger scale.
• FWEC and tapeworm ELISA are imperfect measures of the true parasite burden, but are the best available ante-mortem tests at present. Acknowledgements
• Freedom Health for funding my PhD and providing the test kits
• Dr Tim Parkin • Professor Derek Knottenbelt • The Riding for the Disabled Association
who allowed their horses to be used for this study
• James McGoldrick- Parasitology
[email protected] University of Glasgow, charity number SC004401
An example of a commonly used lateral flow immunoassay
After ‘Lateral Flow Immunoassay’ Springer Publications, Wong and Tse
Conclusions
Albumin was significantly more likely to be detected pre-‐anthelmin6c than post-‐anthelmin6c: P= 0.004
Results- 2 weeks later
N. Kerbyson BVMS Cert AVP (EM) MRCVS, D. Knottenbelt OBE BVM&S DipECEIM MRCVS, T.D.H. Parkin BSc, BVSc, PhD, DipECVPH, FHEA, MRCVS