The II

wl i | iI tl li i ii ~i~,~ ~ ' ~ : . .

BRK-5452

Eppendorf "Ill Repeatef Pipettc Reliable, repetitive pipetting. With the Eppendorf Repeater* Pipette, dispensing up to 48 samples without a refill is a snap. Just set the selection dial for the volume you need and your choice is locked in place to pre- vent errors. That means the last sample will be as accurate and precise as the first. And the unique Combitip 'M potypropylene/ polyethylene reservoir eliminates cleaning, contamination, and carryover because it's disposable.

1-second delivery. The Repeater makes serial pipet- ting procedures faster than ever before. Simply press the lever to deliver your samples at 1-second intervals. The volume range is wide enough to accommodate *U.S. Pat. No, 4406170

almost any procedure. With six Combitip sizes and five dial set- tings, you choose from 22 dif-

ferent volumes be- tween 10 #L and

1%'%~, 5 mL,

\

six Combitip sizes

A wide variety of applications. The Repeater can handle any liquid easily. Even difficult or haz- ardous liquids aren't a problem, since the liquid contacts only the Combitip--not the instrument itself. The Combitip is available

Reader Service No. 135

in nonsterile or sterile packaging for microbiologic and tissue culture techniques. And it can be refilled and re- used as long as the same liquid is being pipetted.

For more information: call 800-645-3050; in New York, 516-334-7500. Or write Brinkmann Instruments, Inc., Cantiague Road, Westbury, NY 11590. (In Canada: 416-675-7911; 50 Galaxy Blvd., Rexdale, Ont. M9W 4Y5)

Shaping the future. Brinkmann I N S T R U M E N T S , I N C .

GENES

DEVELOPMENT A journal devoted to the molecular analysis of gene expression in eukaryotes,

prokaryotes, and viruses

VOLUME 2 NUMBER 12a PAGES 1513-1686 DECEMBER 1988

EDITORIAL BOARD

J. Adams (Melboume, Australia) M. Akam {Cambridge, UK) H. Arst (London, UK) M. Ashburner {Cambridge, UK) J. Beckwith (Boston, USA) W. Bender (Boston, USA} P. Berg {Stanford, USA) T. Cech {Boulder, USA) P. Chambon (Strasbourg, France) N.-H. Chua (New York, USA) J. Dahlberg {Madison, USA) B. Daneholt (Stockholm, Sweden) E. Davidson (Pasadena, USA) K. Davies (Oxford, UK) E. De Robertis (Los Angeles, USA) H. Diggelmann {Lausanne, Switzerland) G. Fink (Cambridge, USA) R. Flavell [Norwich, UK) P. Goodfellow (London, UK) T. Graf (Heidelberg, FRG) W. Herr {Cold Spring Harbor, USA)

EDITORIAL OFFICES

United States Cold Spring Harbor Laboratory Box 100 Cold Spring Harbor, New York 11724 Phone 516-367-8492 FAX 516-367-8532

J. Hodgkin (Cambridge, UK) B. Hogan {Nashville, USA) D. Hopwood (Norwich, UK] R. Horvitz (Cambridge, USA) N. Le Douarin (Nogent sur Marne,

France) J. Manley (New York, USA) K. Matsubara (Osaka, Japan) D. McClay {Durham, USA) S. McKnight {Baltimore, USA) E. Meyerowitz {Pasadena, USA) J. Miller (Los Angeles, USA) N. Murray (Edinburg, UK) S. Numa (Kyoto, Japan) C. N/isslein-Volhard (Tubingen, FRG) T. Okada (Okazaki, Japan) R. Palmiter {Seattle, USA) U. Pettersson {Uppsala, Sweden) W. Schaffner (Zurich, Switzerland) M. Scott (Boulder, USA) L. Shapiro (New York, USA)

B. Shilo (Rehovot, Israel) D. Solter {Philadelphia, USA) J. Strathern (Frederick, USA) H. Varmus {San Francisco, USA) E. Wagner (Heidelberg, FRG) V. Walbot (Stanford, USA) M. Weiss (Paris, France) N. Willetts (Sydney, Australia) J. Witkowski {Cold Spring Harbor, USA)

Founding Editor S. Prentis (1951-1987)

Editors G. Bulfield {Edinburgh) M. Mathews {Cold Spring Harbor)

Associate Editor Terri Grodzicker (Cold Spring Harbor)

Managing Editor J. Cuddihy (Cold Spring Harbor)

United Kingdom AFRC Institute of Animal Physiology and Genetics Research Edinburg Research Station Roslin, Midlothian EH25 9PS, UK Phone 031-440-0977

GENES & DEVELOPMENT (ISSN 0890-9369) is published monthly for $220 {institutional), $90 (individual making personal pay- ment), $50 {Genetical Society of Great Britain members)by Cold Spring Harbor Laboratory, Bungtown Road, Cold Spring Harbor, New York 11724, in association with the Genetical So- ciety of Great Britain. Second-class postage is pending at Cold Spring Harbor and additional mailing offices. POSTMASTER: Send address changes to Cold Spring Harbor Laboratory, POB 100, Cold Spring Harbor, New York 11724. Subscription P~ee Orders may be sent to Cold Spring Harbor Laboratory 1 Fulfillment Department, Bungtown Road, Cold Spring Harbor, New York 11724. Telephone: Continental US except NY State, 1-800-843-4388; all other locations, 516-367- 8325. Volume 2, 1988, $220.00. Personal subscription rate, $90.00. Genetical Society members, $50.00. Price includes sur- facepostage; for airmail, add $35.00. All subscriptions are en- tered for the calendar year and must be prepaid. Personal sub- scriptions must be prepaid by personal check, credit card, or money order. All checks must be for US dollars and drawn on a US bank. Genetical Society members may also subscribe by check, payable to the Genetical Society, for £33 + £8 (surface) or £30 (airlift). Send to: G. Bulfield, AFRC Institute of Animal Physiology and Genetics Research, Roslin, Midlothian EH25 9PS, UK.

Claims for missing issues must be received within 4 months of issue date.

Change of address Please enclose recent mailing label with address change; allow 4 weeks.

Advertising To advertise in Genes & Development, contact Elaine Gaveglia, Advertising Manager, Cold Spring Harbor Lab- oratory, Cold Spring Harbor, New York, 11724; phone 516-367- 8351.

Photo Copy Authorization to photocopy items for internal or personal use, or the internal or personaIuse of specific clients, is granted by Cold Spring Harbor Laboratory for libraries and other users registered with the Copyright Clearance Center [CCC) Transactional Reporting Service, provided that the base fee of $1.00 per copy is paid directly to CCC, 21 Congress St., Salem, MA 01970 (0809~9369/88 $1.00 + 0.). This consent does not extend to other kinds of copying, such as copying for gen- eral distribution for advertising or promotional purposes, for creating new collective works, or for resale.

Copyright © 1988 by Cold Spring Harbor Laboratory

Contents

Letter to the editor The POU domain: a large conserved region in the mammalian pit-l, oct-l, oct-2, and Caenorhabditis elegans unc-86 gene products Winship Herr, Richard A. Sturm, Roger G. Clerc, Lynn M. Corcoran, David Baltimore, Phillip A. Sharp, Holly A. Ingraham, Michael G. Rosenfeld, Michael Finney, Garry Ruvkun, and H. Robert Horvitz

Research papers

cAMP acts in synergy with E1A protein to activate transcription of the adenovirus early genes E4 and E1A Daniel A. Engel, Stephen Hardy, and Thomas Shenk

Induction of proto-oncogene los transcription through the adenylate cyclase pathway: characterization of a cAMP-responsive pathway Paolo Sassone-Corsi, Jane Visvader, Louis Ferland, Pamela L. Mellon, and Inder M. Verma

Isolation and characterization of Drosophila cAMP-dependent protein kinase genes Daniel Kalderon and Gerald M. Rubin

Cloning and expression of AP-2, a cell-type-specific transcription factor that activates inducible enhancer elements Trevor Williams, Arie Admon, Bernhard Lfischer, and Robert Tjian

The B-cell-specific Oct-2 protein contains POU box- and homeo box-type domains Roger G. Clerc, Lynn M. Corcoran, Jonathan H. LeBowitz, David Baltimore, and Phillip A. Sharp

The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain Richard A. Sturm, Gokul Das, and Winship Herr

Control elements of the P2 promoter of the Antennapedia gene Anne M. Boulet and Matthew P. Scott

In vitro analysis of the Antennapedia P2 promoter: identification of a new Drosophila transcription factor Karen K. Perkins, Gina M. Dailey, and Robert Tjian

Alternative splicing of tropomyosin pre-mRNAs in vitro and in vivo David M. Helfman, William M. Ricci, and Linda A. Finn

Isolation and characterization of a novel trophoblast-specific cDNA in the mouse Kathryn R. Lescisin, Susannah Varmuza, and Janet Rossant

A mouse gene homologous to the Drosophila gene caudal is expressed in epithelial cells from the embryonic intestine Philippe Duprey, Kamal Chowdhury, Gregory R. Dressier, Rudi Balling, Dominique Simon, Jean-Louis Guenet, and Peter Gruss

1513

1517

1529

1539

1557

1570

1582

1600

1615

1627

1639

1647

(over)

Molecular analysis of the swallow gene of Drosophila melanogaster Edwin C. Stephenson, Yu-Chan Chao, and lames D. Fackenthal

Molecular characterization of daughterless, a Drosophila sex determination gene with multiple roles in development Claire Cronmiller, Paul Schedl, and Thomas Cline

A link between cell movement and gene expression argues that motility is required for cell--cell signaling during fruiting body development Lee Kroos, Patricia Hartzell, Karen Stephens, and Dale Kaiser

1655

1666

1677

Cover In situ hybridization of Cadl to mouse embryos. Dark-field image of sagittal section through the gut of a 17-day p.c. embryo showing multiple transverse and longitudinal sections through the small intestine. (For details, see Duprey et al., p. 1647.)