The Daly Lab

Personnel:Postdoctoral:

•Erich Staudacher Graduate students:

•Oakland Peters•Esther Mwilaria (MS Sp 07)

Undergraduate students: •Faizan Kalwar •Mandy Hatfield •Morgan Bridi •Tyler Pittman•Aarzu Moghal •Sawan Phrabhu

Current proposals:• National Institutes of Health (R01) – NIDCD “The role of

periodic input in odor coding” PI: Kevin C. Daly 5 years; (initial score 228; in review).

• Human Frontiers in Science. “The virtual 4D antennal lobe: Representation of odor processing in space at spike-time resolution. Score: 30th (in revision for NSF ETA spring 09).

Prior funding:• National Institutes of Health (R03) – NIDCD: “Analysis of

combinatorial olfactory coding” Grant number: DC05535; 3 years, $221,000.

• McDonnell Foundation collaborative grant: “Hydrodynamic and neural filtering of spatial information in a turbulent odor plume” 3 years $105,000.

• National Institutes of Health (R01) – NIDCD “Aminergic modulation underlying olfactory plasticity” PI: Thomas A Christensen 5 years; OSU subcontract: $200,000 Grant number: DC05652.

• National Institutes of Health (P20) – NCRR “COBRE in Sensory Neuroscience” PI George Spirou; CoPI: Kevin C. Daly (RR015574-06). 5 year $10,724,104.00: Daly sub award: $1,354,457.00

Wing beating causes oscillating airflow over the antennae

Sane and Jacobson JEB 2006

Indu

ced

airs

peed

(m

/s)

Rel

ativ

e am

plitu

de

EA

G D

efle

ctio

n (m

V)

Peri-stimulus Time (sec)

2

1

0

-1

-2

2

0

-2

4

-4

i2

1

0

-1

-2

2

1

0

-1

-2

2

0

-2

4

-4

2

0

-2

4

-4

i

-.5 0 .5 1 1.5 2 2.5 3 3.5-.5 0 .5 1 1.5 2 2.5 3 3.5-.5 0 .5 1 1.5 2 2.5 3 3.5

2

1

0

-1

-2

4

0

-4

8

-8

ii2

1

0

-1

-2

2

1

0

-1

-2

4

0

-4

8

-8

4

0

-4

8

-8

ii

20 Hz

Continuous

Frequency (Hz)

Raw

Po

we

r (m

V2 /

Hz*

10-

3 )

10 20 30 40 5010 20 30 40 50

10

5

0

10

5

0

i

16 18 20 2216 18 20 22

ii

10 15 18 20 22 25 30 34 4010 15 18 20 22 25 30 34 40

4.5

3.5

2.5

1.5

.5

-.5

4.5

3.5

2.5

1.5

.5

-.5

Stimulation frequency (Hz)A

vera

ge

po

wer

(z-

scor

e) *̂*̂

*̂*̂*̂*̂ *̂*̂ *̂*̂ *̂*̂

^̂

Oscillating odor stimulus results in oscillating electroantennogram (EAG) up to ~30Hz

RAW and 5Hz high pass filtered EAG

Power spectral densityIntegrated power

Mean integrated powerpulsed odorpulsed blank3s cont. odor

Oscillating stimulus results in oscillating local field potentials in the antennal lobe up to 72 Hz

Frequency (Hz)

Raw

Po

wer

(m

V2 /

Hz*

10-3)

10 20 30 40 5010 20 30 40 50

2

1

0

2

1

016 18 20 22

i ii

10 14 18 20 22 25 30 34 4010 14 18 20 22 25 30 34 40

1.5

.5

-.5

1.5

.5

-.5

Stimulation frequency (Hz)M

ean

pow

er (

z-sc

ore)

**

*̂*̂

^̂

^̂ *̂*̂^̂ *̂*̂ *̂*̂ *̂*̂

Ext

race

llula

r Pot

entia

l (m

V)

Peri-stimulus Time (sec)

2

1

0

-1

-2

2

1

0

-1

-2

i

2

1

0

-1

-2

2

1

0

-1

-2-.5 0 .5 1 1.5 2 2.5 3 3.5-.5 0 .5 1 1.5 2 2.5 3 3.5

ii

2

0

3

1

-1

-2

-3

2

0

3

1

-1

-2

-3

2

0

3

1

-1

-2

-3

2

0

3

1

-1

-2

-3

i 20 Hz

Continuous

Mean integrated powerpulsed odorpulsed blank3s cont. odor

Peri-stimulusTime (sec)

Pro

babi

lity

0 0.2 0.4 0.6 0.8 1 1.2Time (sec)

00.10.20.3

Pro

babili

ty

0 .2 .4 .6 .8 1 1.2

0.1.2.3

Odo

r

A6A7A8A9

A10K6K7K8K9

K10B 10 20 30 40 50 10 20 30 40 50

Mea

n P

ower

(z-

scor

e) 1

.5

0

-.5

-1

Frequency (Hz)

i ii

Uni

ts R

espo

ndin

g (%

)10 14 18 20 22 25 30 34 40

Stimulation frequency (Hz)

50

40

30

20

10

0

stim

Oscillating stimulus results in oscillating unitary spiking from antennal lobe neurons up to ~40 Hz (extracellular)

Oscillating stimulus results in oscillating unitary spiking from antennal lobe neurons up to only ~15 Hz (intracellular)

1

0

-1

1

0

-1

10 20 30 40 5010 20 30 40 50Mea

n P

ow

er (

z-sc

ore)

Frequency (Hz)

10 20 30 40 5010 20 30 40 50Mea

n P

ow

er (

z-sc

ore)

1

0

-1

1

0

-1

Frequency (Hz)

Local interneuron

Projection neuron

GABAA disruption results in loss of oscillating responses from antennal lobe LFP and unitary spiking

10 20 30 40 465

50

40

30

20

10

0

50

40

30

20

10

0Uni

ts R

espo

ndin

g (%

)

Stimulation Frequency (Hz)

Loss of unitary oscillatory responses

10 20 30 40 46 57 7210 20 30 40 46 57 72

.8

.6

.4

.2

0

-.2

-.4

-.6

.8

.6

.4

.2

0

-.2

-.4

-.6Mea

n po

wer

(z-

scor

e)

Stimulation Frequency (Hz)

* * * * * * *

-.5 0 .5 1 1.5

100

80

60

40

20

0

100

80

60

40

20

0

Peri-stimulus Time (sec)M

ean

pow

er (

z-sc

ore)

Fre

quen

cy (

Hz)

i

ii

10

0

8

6

4

2

10

0

8

6

4

2

pre bicucullinepre

bicuculline

Reconstructing whole antennal lobe output responses by serial reconstruction

Virtual population responses from confirmed PN in response to an odor panel