The CRISPR-Cas system · 2019-10-23 · diapositiva. Sección Diapositivas Transiciones ......
Transcript of The CRISPR-Cas system · 2019-10-23 · diapositiva. Sección Diapositivas Transiciones ......
Francisco J. M. Mojica
The CRISPR-Cas system
cas genes(CRISPR associated )
Cas proteins
C R I S P R
CRISPR-Cas System
cas genes(CRISPR associated )
Cas proteins
Immunity
C R I S P R
CRISPR-Cas System
Infection
Immunization
Immunized Bacterium
Guides Production
crRNA
Reinfection
Target Recognition
Target Cleavage
Target Inactivation
Makarova et al. Nat. Rev. Microbiol. 2011
Diversity of CRISPR-Cas
Makarova et al. Nat. Rev. Microbiol. 2015
3 Types; 10 Subtypes5 Types; 16 Subtypes
From: Mobile Genetic Elements and Evolution of CRISPR-Cas Systems: All the Way There and Back. Genome Biol Evol. 2017;9(10):2812-2825.
Diversity of CRISPR-Cas2 Classes; 6 Types; 30 Subtypes
Applications of CRISPR-Cas in prokaryotes
1st Application (typing of pathogenic bacteria)
Isolate 1
Isolate 2
Isolate 4
Isolate 3
Protection
Genetic Barrier
Data storage/recording
IIIIIIIII
Cas9
Guide RNA
IIIIIIIIIIIIIIIIIIIIIIIIIII
PAMDNA
IIIIIIIII
Cas9
Guide RNA
DNA IIIIIIIIIIIIIIIIIIIIIIIIIIIPAM
Antimicrobials
Selection of recombinant Bacteria
Jiang et al. Nat Biotechnol. 2013
Applicable to Plants
Zhang, D., et al., Targeted Gene Manipulation in Plants Using the CRISPR/Cas Technology, Journal of Genetics and Genomics(2016), http://dx.doi.org/10.1016/j.jgg.2016.03.001
Mojica & Montoliu, 2016
Published: April 8, 2019