THE “ROAD MAP”anatomical-sciences.health.wits.ac.za/roadmaps/Muscle...MUSCLE • Skeletal...

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PRACTICAL ROADMAP MUSCLE WJ van der Spuy

Transcript of THE “ROAD MAP”anatomical-sciences.health.wits.ac.za/roadmaps/Muscle...MUSCLE • Skeletal...

Page 1: THE “ROAD MAP”anatomical-sciences.health.wits.ac.za/roadmaps/Muscle...MUSCLE • Skeletal (striated) • Cardiac (striated) • Smooth • Identification based on – Fiber size,

PRACTICAL ROADMAP MUSCLE

WJ van der Spuy

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MUSCLE

• Skeletal (striated) • Cardiac (striated) • Smooth

• Identification based on

– Fiber size, shape and presence/absence of branching – Number, shape and position of nuclei – Presence or absence of cross-striations

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MUSCLE Skeletal Cardiac Smooth

Cell Elongated Branching Spindle-shaped

Nucleus Multiple peripheral Single or bi-nucleated central

Single central

Striations Cross-striated Cross-striated & Intercalated disks

None

Motor Control Voluntary Involuntary Involuntary

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SKELETAL MUSCLE

• Slide 76 Striated Skeletal Muscle – Tongue – Iron haematoxylin stain

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Organisation

Epimysium - surrounding muscle

Perimysium - surrounding fascicle

Endomysium - surrounding muscle fibre

Scanning Electron Micrograph – WJ van der Spuy – MSc

100µm

Muscle fibre

Fascicle

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Fibre Orientation

Longitudinal section

Transverse section

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Identification

Muscle fibre

Muscle fibre

Multiple peripheral nuclei

Cross-striations Fibroblast nuclei in connective tissue

sheath

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Striations

A band (dark striation) bisected by light H band

I band (light striation) bisected by dark Z line

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SKELETAL MUSCLE

• Slide 50 Musculo-tendinous junction – H&E stain

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Musculo-tendinous Junction

Dense regular connective tissue Skeletal Muscle

Musculo-tendinous junction with fibres inserting obliquely into tendon

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SKELETAL MUSCLE

• Slide 4 Neuromuscular (myoneural) junction – Snake – H&E and gold impregnation staining

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Neuromuscular Junction

Axon Boutons (neuromuscular junction)

Motor Nerve (neuromuscular bundle in longitudinal section) Striated skeletal

muscle fibres

Neuromuscular bundle (transverse section)

Micrograph – WJ van der Spuy – MSc Stain: Toluidine blue pH=11.5

Blood vessel

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Neuromuscular Junction

Axon boutons

Motor nerve

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Muscular Dystrophy

• Limb-Girdle Muscular Dystrophy type 2B (LGMD2B)

• Increased rate of regeneration in response to ongoing degeneration of muscle

• Characterised by: – Variation in fiber size – Necrosis of muscle tissue – Revascularisation – Inflammatory cell infiltration – Phagocytosis of necrotic debris – Proliferation of mononuclear muscle precursors – Fusion of precursors to form myotubes

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LGMD2B CN: Central nucleus Regenerating fibre

FS: Fibre splitting Early hyperplasia

FL: Fibre lobulation Late hyperplasia

LI: Leukocyte infiltration Inflammation

NF: Necrotic fibre Degenerating fibre

PI: Postnecrotic infiltrate Clearing debris after degeneration

SC: Satellite cell Mononuclear muscle precursor – regenerative ability

PN: Peripheral nucleus Mature fibre

Micrograph – WJ van der Spuy – MSc Stain: Toluidine blue pH=11.5

CN FL

PI

PN

FS

NF

SC 10µm

LI

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CARDIAC MUSCLE

• Slide 57 Heart (interventricular septum) – Iron haematoxylin stain

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Fibre Orientation

Longitudinal section Transverse section

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Identification

Cross-striations

Fibre branching

Intercalated discs

Central nuclei

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Interventricular Septum

Endocardium

Purkinje fibres

Myocardium with ordinary cardiac muscle fibres

Capillaries with black stained RBCs

Lumen of left ventricle

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Purkinje Fibres

Pale-staining fibres

Large glycogen space around nuclei

Nucleus

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Ordinary Cardiac Muscle Fibres

Dark-staining fibres

Smaller glycogen spaces around nuclei

Nucleus

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CARDIAC MUSCLE

• Slide 86 Heart – H&E and elastic stain

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Interventricular Septum

Endocardium

Purkinje fibres

Myocardium with ordinary cardiac muscle fibres

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Interventricular Septum

Purkinje fibres Myocardium with ordinary

cardiac muscle fibres

Large glycogen space around nucleus

Nucleus

Small glycogen space around nucleus

Nucleus

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SMOOTH MUSCLE

• Slide 10 Smooth muscle – Uterus (baboon) – H&E stain

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Overview

Endometrium Myometrium with smooth muscle

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Fibre Orientation

Longitudinal section

Transverse section

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Identification

Cigar-shaped nucleus centrally located in spindle shaped fibre

Longitudinal section

Transverse section

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Smooth Muscle vs. Connective Tissue

Blood vessel with smooth muscle wall

Irregular dense connective tissue

Myometrium with smooth muscle


UNIT 3: MUSCLES. Types of Muscle tissue Skeletal: striated and voluntary- moves body Smooth: involuntary, non-striated Cardiac: heart, involuntary.

UNIT 3: MUSCLES. Types of Muscle tissue Skeletal: striated and voluntary- moves body Smooth: involuntary, non-striated Cardiac: heart, involuntary.

Function depends on structure. Muscle classification 1.Striated muscle A.Skeletal Muscle - voluntary muscles that allow for movement B.Cardiac Muscle.

Function depends on structure. Muscle classification 1.Striated muscle A.Skeletal Muscle - voluntary muscles that allow for movement B.Cardiac Muscle.

MEDICS EMGs MUSCLE - University of the Witwatersrandanatomical-sciences.health.wits.ac.za/dems/Medics-EMGs/MEDICS E… · Microsoft PowerPoint - MEDICS EMGs MUSCLE Author: AnnLab-Admin

MEDICS EMGs MUSCLE - University of the Witwatersrandanatomical-sciences.health.wits.ac.za/dems/Medics-EMGs/MEDICS E… · Microsoft PowerPoint - MEDICS EMGs MUSCLE Author: AnnLab-Admin

2217 – Motor system Somatic – striated muscle Autonomic – smooth muscle, cardiac muscle, glands

2217 – Motor system Somatic – striated muscle Autonomic – smooth muscle, cardiac muscle, glands

Clinically Relevant Anatomy What Drives Us · PDF fileHow Many Types of Muscle? Involuntary Voluntary Striated VS Non Striated ? Striated Striated Non-Striated Functions of Muscle

Clinically Relevant Anatomy What Drives Us · PDF fileHow Many Types of Muscle? Involuntary Voluntary Striated VS Non Striated ? Striated Striated Non-Striated Functions of Muscle

Muscle Types Skeletal: striated, voluntary Cardiac: only in heart, striated, involuntary Smooth/Visceral: walls of organs, not striated, involuntary.

Muscle Types Skeletal: striated, voluntary Cardiac: only in heart, striated, involuntary Smooth/Visceral: walls of organs, not striated, involuntary.

Muscle Three basic types: Skeletal = striated = voluntary Smooth = non-striated = involuntary = visceral Cardiac.

Muscle Three basic types: Skeletal = striated = voluntary Smooth = non-striated = involuntary = visceral Cardiac.

Studies on nebulin in amphioxus striated muscle

Studies on nebulin in amphioxus striated muscle

MUSCLE PHYSIOLOGY (PHS 213) By Emojevwe V. …oer.unimed.edu.ng/LECTURE NOTES/3/3/Emojevwe-V-MUSCLE... · Non-striated Muscle: ... Involuntary Muscle: Muscle that cannot be controlled

MUSCLE PHYSIOLOGY (PHS 213) By Emojevwe V. …oer.unimed.edu.ng/LECTURE NOTES/3/3/Emojevwe-V-MUSCLE... · Non-striated Muscle: ... Involuntary Muscle: Muscle that cannot be controlled

the Heart Muscle Cell ( Cardiac striated muscle )

the Heart Muscle Cell ( Cardiac striated muscle )

Non-striated Muscle I

Non-striated Muscle I

Cardiac Muscle The third category of muscle is cardiac muscle, which makes up most of the heart. Like skeletal muscle, it is striated. Like smooth muscle,

Cardiac Muscle The third category of muscle is cardiac muscle, which makes up most of the heart. Like skeletal muscle, it is striated. Like smooth muscle,

Striated muscle tissue x 400 Muscle histology Notice the fine ‘stripes’ or striations along each muscle cell.

Striated muscle tissue x 400 Muscle histology Notice the fine ‘stripes’ or striations along each muscle cell.

CHAPTER 6 MUSCULAR SYSTEM. MUSCLE CELL TYPES Skeletal (striated) Cardiac Smooth (non-striated)

CHAPTER 6 MUSCULAR SYSTEM. MUSCLE CELL TYPES Skeletal (striated) Cardiac Smooth (non-striated)

Muscle Physiology - ncwcom.comjones/bio121/musclephys.pdf · 2. cardiac- heart muscle, striated, involuntary, intercalated discs 3. smooth- non-striated, involuntary, visceral muscle,

Muscle Physiology - ncwcom.comjones/bio121/musclephys.pdf · 2. cardiac- heart muscle, striated, involuntary, intercalated discs 3. smooth- non-striated, involuntary, visceral muscle,

Non-striated Muscle I. Locations of non-striated muscle in vertebrate body GI tract (including internal, but not external sphincters) Blood vessels and.

Non-striated Muscle I. Locations of non-striated muscle in vertebrate body GI tract (including internal, but not external sphincters) Blood vessels and.

Histology - Deer Park Independent School District / · PDF file3 Types of Muscle • Skeletal muscle – Striated (stripes) – Voluntary ... • Smooth muscle – Visceral ... •

Histology - Deer Park Independent School District / · PDF file3 Types of Muscle • Skeletal muscle – Striated (stripes) – Voluntary ... • Smooth muscle – Visceral ... •

The Muscular System. Structure and Function of Muscle Tissue Muscle type SkeletalCardiacSmooth Striated? Voluntary? Location? Regenerate Function?

The Muscular System. Structure and Function of Muscle Tissue Muscle type SkeletalCardiacSmooth Striated? Voluntary? Location? Regenerate Function?

striated muscle heart muscle smooth muscle excitation contraction coupling.

striated muscle heart muscle smooth muscle excitation contraction coupling.

Workbook 4 The musculoskeletal system - · PDF fileLike skeletal muscle, cardiac muscle contains striated fibres. Cardiac muscle is called involuntary muscle because conscious ...

Workbook 4 The musculoskeletal system - · PDF fileLike skeletal muscle, cardiac muscle contains striated fibres. Cardiac muscle is called involuntary muscle because conscious ...