TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf ·...
Transcript of TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf ·...
![Page 2: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/2.jpg)
OVERVIEW OF BIOSEPARATIONS
• Up to 90% of new product cost is in downstream processing.
• More than 60% of the cost of third and fourth generation antibiotics is in purification.
Bioseparations are elaborate and expensive.
For recombinant DNA fermentation products, downstream processing can account for 80 to 90% of the overall processing cost.
![Page 3: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/3.jpg)
BIOSEPARATION COST
![Page 4: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/4.jpg)
4
To improve the design of bioseparations, the following
questions should be answered:
(1) What is the value of the product?
(2) What is an acceptable product quality?
(3) Where is the product in each process stream?
(4) Where are the impurities in each process stream?
(5) What are the unusual physicochemical properties of
the product and the principal impurities?
(6) What are the economics of various alternative
separations?
![Page 5: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/5.jpg)
BIOPRODUCTS
![Page 6: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/6.jpg)
BIOPRODUCTS
![Page 7: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/7.jpg)
7
Bioproducts can be broadly classified into three categories:
small molecules, large molecules, and particulate products.
There are three corresponding separation procedures for
purifying them.
![Page 8: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/8.jpg)
8
(1) Separation of insolubles
* Objectives: remove or collect cells, cell debris, or
other particulates
* Typical operations: filtration, centrifugation
* Relatively little product concentration or
improvement of product quality occurs.
Most bioseparations have four similar steps:
![Page 9: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/9.jpg)
9
Most bioseparations have four similar steps (2/3):
(1) Separation of insolubles
(2) Isolation of products
* Objectives: remove materials of widely divergent
properties compared to the desired product
* Typical operations: extraction, adsorption,
ultrafiltration, precipitation
* Appreciable concentration and product quality
increases usually occur.
![Page 10: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/10.jpg)
10
Most bioseparations have four similar steps (3/3):
(1) Separation of insolubles
(2) Isolation of products
(3) Purification
* Objectives: remove impurities of similar chemical
functionality and physical properties
* Typical operations: chromatography, affinity
methods, fractional precipitation, electrophoresis
(4) Polishing
* Objectives: remove liquids
* Typical operations: drying, crystallization (not
always possible)
![Page 11: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/11.jpg)
11
Processing profile of antibiotics:
Product
Step Typical Process Conc.
(g/L)
Quality
(%)
Harvest broth Fermentation 0.1-5 0.1-1.0
Separation of
insolubles
Filtration 1.0-5 0.2-2.0
Isolation Extraction 5-50 1-10
Purification Chromatography 50-200 50-80
Polishing Crystallization 50-200 90-100
![Page 12: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/12.jpg)
12
Separation principles used in various recovery steps:
![Page 13: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/13.jpg)
Size • Filtration
• Membrane
Density
• Sedimentation
• Centrifugation
• Flotation
Diffusivity
• Membrane
Shape • Filtration
• Sedimentation
• Centrifugation
![Page 14: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/14.jpg)
Polarity
• Extraction
• Chromatography
• Adsorption
Solubility
• Extraction
• Precipitation
• cristallisation
Elc. charge
• Adsorption
• Electrophoresis
Volatility
• Distillation
• Pervaporation
![Page 15: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/15.jpg)
Bioseparation Technique
• According to product characteristic
• Considering selectivity and productivity
• Selectivity: main product/byproduct
• Productivity: main product/time
![Page 16: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/16.jpg)
Bioseparation Technique
• Low selectivity-high productivity
• Cell disruption
• Precipitation
• Centrifugation
• Extraction
• Leaching
• Filtration (+micro, ultra)
• Adsorption
• High selectivity-low productivity
• Ultracentrifugation
• Chromatography
• Affinity separation
• electrophoresis
![Page 17: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/17.jpg)
EXAMPLE
![Page 18: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/18.jpg)
18
![Page 19: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/19.jpg)
19
Process for ethanol production from fermentation:
![Page 20: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/20.jpg)
20
Manufacture of citric acid:
![Page 21: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/21.jpg)
21
Penicillin production:
![Page 22: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/22.jpg)
22
Production of an intracellular enzyme:
![Page 23: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/23.jpg)
23
Processing scheme for recombinant insulin:
(Inclusion Body)
![Page 24: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/24.jpg)
24
Processing scheme for recombinant insulin (2/2):
![Page 25: TEKNIK SEPARASI - istianahnuristianah.lecture.ub.ac.id/files/2017/02/TEKNIK-SEPARASI-1.pdf · TEKNIK SEPARASI NUR ISTIANAH,ST.MT.,M.Eng . OVERVIEW OF BIOSEPARATIONS ... 12 Separation](https://reader030.fdocuments.us/reader030/viewer/2022040103/5f08f47e7e708231d42488af/html5/thumbnails/25.jpg)
THANKS FOR YOUR ATTENTION
The best person is one give something useful always