Techniques..

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1) The damage caused by ultraviolet radiation is 1) Never repaired. 2) Repaired by DNA replication. 3) Cut out and replaced. 4) Repaired during transcription. 5) Repaired during translation. 2) The following steps are used to make DNA fingerprints. What is the third step? 1) Perform electrophoresis. 2) Collect DNA. 3) Add stain. 4) Digest with a restriction enzyme. 5) Lyse cells. 3) The reaction catalyzed by reverse transcriptase. mRNA rightarrow cDNA DNA rightarrow DNA DNA rightarrow mRNA

Transcript of Techniques..

Page 1: Techniques..

1) The damage caused by ultraviolet radiation is

1) Never repaired.

2) Repaired by DNA replication.

3) Cut out and replaced.

4) Repaired during transcription.

5) Repaired during translation.

2) The following steps are used to make DNA fingerprints. What is the third step?

1) Perform electrophoresis.

2) Collect DNA.

3) Add stain.

4) Digest with a restriction enzyme.

5) Lyse cells.

3) The reaction catalyzed by reverse transcriptase.

15) mRNA rightarrow cDNA

15) DNA rightarrow DNA

15) DNA rightarrow mRNA

15) mRNA rightarrow protein

15) None

4) Advancement in genetic engineering has been possible due to the discovery of

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1) Oncogenes

2) Transposons

3) Restriction endonuclease

4) Exonucleases

5) Agarose extracted from sea weeds finds use in

1) Gel electrophoresis

2) Spectrophotometry

3) Tissue culture

4) PCR

6) DNA fingerprinting was discovered by

1) James Watson

2) Alec Jeffreys

3) Frederick Fanger

4) Hargobind Khorana

7) DNA is found in

1) Chromosomes

2) Plastids

3) Mitochondria

4) All of the above

8) Dolly, The first cloned mammal, was produced by

1) Parthenogenesis

2) Artificial fertilization

3) Replacement of zygote nucleus by somatic nucleus

4) in vitro fertilization

9) First artificial gene was synthesized by

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1) Nirenberg

2) Mendel

3) Morgan

4) Khorana

10) Genetic Engineering is

1) plastic surgery

2) addition

3) study of extranuclear genes

4) all of the above

11) Genomics is the study of

1) Genes in general

2) Human genes

3) Genomes in general

4) Human genome

12) Given below is a sample of a portion of DNA strand giving the base sequence on the

opposite strands. What is so special shown in it? 5' ______ GAATTC ________ 3' 3'

________CTTAAG ________ 5'

1) Palindromic sequence of base pairs

2) Replication completed

3) Deletion mutation

4) Start codon at the 5' end

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13) Maximum number of existing transgenic animals is of

1) Pig

2) Fish

3) Mice

4) Cow

14) One of the following processes involves southern blotting technique

1) rDNA technology

2) DNA finger printing

3) production of transgenic plants

4) formation of suspension culture

15) Restriction endonucleases are used in genetic engineering and they

1) cut DNA at various sites

2) join DNA segments

3) cut RNA at specific sites

4) cut DNA at specific sites

16) Cations have:

1) Positive charge

2) Negative charge

3) No charge

4) It is impossible to predict the charge on a cation

17) Which of the following is NOT a laboratory safety rule?

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1) You should never mix acids with bases

2) You should tie back your long hair

3) You should never add water to acid

4) All of the above are valid safety rules

18) What piece of laboratory equipment is best-suited for accurately measuring the

volume of a liquid?

1) graduated cylinder

2) beaker

3) Erlenmeyer flask

4) more than one of the above

19) Which piece of laboratory equipment can be used to store chemicals for long

periods of time?

1) buret

2) evaporating dish

3) beaker

4) more than one of the above

20) What is the appropriate SI unit for distance?

1) centimeters

2) inches

3) meters

4) kilometers

21) An example of a chemical property is:

1) density

2) mass

3) acidity

4) solubility

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22) Mass spectrometers separate isotopes of different elements based on their:

1) mass

2) electric charge

3) mass divided by electric charge

4) none of these

23) Chromatography is used to: bas

1) Separate two or more compounds ed on their polarities.

2) Separate two or more compounds based on their masses.

3) Separate two or more compounds based on how strongly they interact with other

compounds.

4) More than one of the above.

24) An unsaturated solution:

1) Hasn’t dissolved as much solute as is theoretically possible

2) Has dissolved exactly as much solute as is theoretically possible

3) Is unstable because it has dissolved more solute than would be expected.

4) none of these

25) If I dilute 5 mL of 0.15 M NaCl to a final volume of 5 L, what’s the final

concentration of NaCl?

1) 0.00015 M

2) 0.0015 M

3) 15000 M

4) none of these

26) What’s the molality if I have 5 L of a solution that contains 1.5 moles of lithium

acetate.

1) 1.5 m

2) 3.33 m

3) 0.30 m

4) none of these

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27) Which of the following is not an acid?

1) HNO3

2) CH3COOH

3) H2SO4

4) All of these are acids

1) 3

2) 4

3) 1

4) 3

5) 1

6) 2

7) 4

8) 3

9) 4

10) 2

11) 3

12) 1

13) 3

14) 3

15) 4

16) 1

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17) 1

18) 1

19) 3

20) 3

21) 3

22) 3

23) 4

24) 1

25) 1

26) 4

27) 4

28)