Targeting of MCF-7 Breast Cancer Cells using MTBs · Targeting of MCF-7 Breast Cancer Cells using...
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Targeting of MCF-7 Breast Cancer Cells using MTBs We investigate the sensing capabilities of magnetotactic bacteria (Magnetospirillum gryphiswaldense strain MSR-1) to MCF-7 breast cancer cells. Microfluidic chips with a three-way junction are designed and fabricated. These chips contains one inlet and two chambers to insert the bacteria and to contain the cancer cells, respectively. Objective In this project, we will do experiment using magnetotactic bacteria (MTBs), fluidic microchips with three-way junction, and cancer cells. The ultimate goal of the experiment is to investigate the sensing capabilities of MTBs to cancer cells. MTBs have long been known to swim towards environment with relatively low oxygen content. Cancer cells influence the oxygen gradient locally, and therefore we expect that MTBs will swim towards the cells. Tasks • Development of a feature tracking algorithm to count the number of MTBs that swim through the three-way junction Fig. 1; • Tracking of multiple bacteria to provide quantitative information on the culture; • Collecting experimental data to investigate the sensing capabilities of the MTBs to the cancer cells. Materials • 4 electromagnetic coils are available in MNRLab; • 4 electric drivers; • Motion control systems; • A microfluidic chip with three-way junction; • MCF-7 breast cancer cells. PREREQUISITES Students are expected to have a working knowledge of control theory, differential equations, linear systems, statics, kinematic and dynamics. Familiarity with programming, especially with Matlab and C++. OTHER NOTES This project will involve a weekly meeting with the instructors and progress reports have to be prepared. All reports should be written in academic paper format. 1. References [1] R. Blackemore, Magnetotactic bacteria, Science, 190 (4212), pp. 377–379, October 1975. Figure 1. Magnetotactic bacteria (MTB) are inserted in the fluidic chip to select between the left and right chambers. (a) The three-way junction allows us to observe the motion of the MTB towards the left and the right chambers. (b) A few hundreds of MTB are observed in the channel connected to the chamber of the MCF-7 breast cancer cells. (c) A few MTB are observed along the channel connected to the chamber of the cancer cell medium. (d) The left chamber contains cancer cells. (e) The right chamber contains cancer cell medium only. [2] A. S. Bahaj, P. A. B. James, and F. D. Moeschler, An alternative method for the estimation of the magnetic moment of non-spherical magnetotactic bacteria, IEEE Transactions on Magnetics. vol. 32, no. 5, pp. 5133-5135, September 1996. [3] M. Elfar, M. Ayoub, A. Sameh, H. Abass, R. M. Abdel-Kader, I. Gomaa, and I. S. M. Khalil, “Targeted penetration of MCF-7 cells using iron-oxide nanoparticles in vitro,” in Proceedings of the IEEE RAS/EMBS International Conference on Biomedical Robotics and Biomechatronics (BioRob), pp. 260-265, Singapore, June 2016. [4] H. A. Hassan, M. Pichel, T. Hageman, L. Abelmann, and I. S. M. Khalil, “Influence of the magnetic field on the two-dimensional control of magnetospirillum gryphiswaldense strain MSR-1," in Proceedings of the IEEE/RSJ International Conference of Robotics and Systems (IROS), pp. 5119–5124, Daejeon, Korea, October 2016. [5] A. Hosney, J. Abdalla, I. S. Amin, N. Hamdi, and I. S. M. Khalil, “In vitro validation of clearing clogged vessels using microrobots," in Proceedings of the IEEE RAS/EMBS International Conference on Biomedical Robotics and Biomechatronics (BioRob), pp. 272-277, Singapore, June 2016. www.MNRLab.com Osama Mosleh, Nermeen Serag, and Mohamed Elwi, and Islam S. M. Khalil 1
Transcript of Targeting of MCF-7 Breast Cancer Cells using MTBs · Targeting of MCF-7 Breast Cancer Cells using...
Targeting of MCF-7 Breast Cancer Cells using MTBs
We investigate the sensing capabilities of magnetotactic bacteria (Magnetospirillum gryphiswaldense strain MSR-1) toMCF-7 breast cancer cells. Microfluidic chips with a three-way junction are designed and fabricated. These chipscontains one inlet and two chambers to insert the bacteria and to contain the cancer cells, respectively.
ObjectiveIn this project, we will do experiment using magnetotacticbacteria (MTBs), fluidic microchips with three-wayjunction, and cancer cells. The ultimate goal of theexperiment is to investigate the sensing capabilities ofMTBs to cancer cells. MTBs have long been known toswim towards environment with relatively low oxygencontent. Cancer cells influence the oxygen gradient locally,and therefore we expect that MTBs will swim towards thecells.
Tasks• Development of a feature tracking algorithm to count
the number of MTBs that swim through the three-wayjunction Fig. 1;
• Tracking of multiple bacteria to provide quantitativeinformation on the culture;
• Collecting experimental data to investigate the sensingcapabilities of the MTBs to the cancer cells.
Materials• 4 electromagnetic coils are available in MNRLab;
• 4 electric drivers;
• Motion control systems;
• A microfluidic chip with three-way junction;
• MCF-7 breast cancer cells.
PREREQUISITESStudents are expected to have a working knowledgeof control theory, differential equations, linear systems,statics, kinematic and dynamics. Familiarity withprogramming, especially with Matlab and C++.
OTHER NOTESThis project will involve a weekly meeting with theinstructors and progress reports have to be prepared. Allreports should be written in academic paper format.
1. References[1] R. Blackemore, Magnetotactic bacteria, Science, 190
(4212), pp. 377–379, October 1975.
Figure 1. Magnetotactic bacteria (MTB) are inserted in the fluidicchip to select between the left and right chambers. (a) Thethree-way junction allows us to observe the motion of the MTBtowards the left and the right chambers. (b) A few hundreds ofMTB are observed in the channel connected to the chamber ofthe MCF-7 breast cancer cells. (c) A few MTB are observed alongthe channel connected to the chamber of the cancer cell medium.(d) The left chamber contains cancer cells. (e) The right chambercontains cancer cell medium only.
[2] A. S. Bahaj, P. A. B. James, and F. D. Moeschler, Analternative method for the estimation of the magneticmoment of non-spherical magnetotactic bacteria,IEEE Transactions on Magnetics. vol. 32, no. 5,pp. 5133-5135, September 1996.
[3] M. Elfar, M. Ayoub, A. Sameh, H. Abass,R. M. Abdel-Kader, I. Gomaa, and I. S. M.Khalil, “Targeted penetration of MCF-7 cells usingiron-oxide nanoparticles in vitro,” in Proceedingsof the IEEE RAS/EMBS International Conference onBiomedical Robotics and Biomechatronics (BioRob),pp. 260-265, Singapore, June 2016.
[4] H. A. Hassan, M. Pichel, T. Hageman, L. Abelmann,and I. S. M. Khalil, “Influence of the magnetic fieldon the two-dimensional control of magnetospirillumgryphiswaldense strain MSR-1," in Proceedings of theIEEE/RSJ International Conference of Robotics andSystems (IROS), pp. 5119–5124, Daejeon, Korea,October 2016.
[5] A. Hosney, J. Abdalla, I. S. Amin, N. Hamdi,and I. S. M. Khalil, “In vitro validation of clearingclogged vessels using microrobots," in Proceedingsof the IEEE RAS/EMBS International Conference onBiomedical Robotics and Biomechatronics (BioRob), pp.272-277, Singapore, June 2016.
www.MNRLab.com Osama Mosleh, Nermeen Serag, and Mohamed Elwi, and Islam S. M. Khalil 1
