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NEW ALLE LE ALERT S

Identification of the new C*07:555 allele in an unrelated donorfor HSCT

AQ1 R. Condello | M. Battarra | M. Testi | G. Testa | M. Andreani

Laboratory of Immunogenetics and TransplantBiology, IME Foundation at Polyclinic of TorVergata, Rome, Italy

CorrespondenceRossella Condello, Laboratory of Immunogeneticsand Transplant Biology, IME Foundation atPolyclinic of Tor Vergata, Viale Oxford 81, Rome,Italy.Email: r.condello@fondazioneime.org

HLA-C*07:555 differs from C*07:01:01:01 by a C to G substitution in exon 2.

KEYWORDS

HLA-C*07:555, new allele, sequence-based typing

AQ2 We describe the identification of a new allele, belonging tothe HLA-C*07 group, discovered during the human leuko-cyte antigen (HLA) typing of an unrelated hematopoieticstem cell donor. Genomic DNA was isolated from periph-eral blood, using an automatic instrument, according to themanufacturer’s instructions (Maxwell Promega, Madison,Wisconsin). The DNA of the potential donor was subse-quently typed at high-resolution level, as required by thespecific protocols, shared between our laboratory and thetransplant center, for the HLA-A, -B, -C, -DRB1, -DRB3,-DRB4, -DQA1, -DQB1 and -DPB1 loci. The first step ofHLA typing was performed using a sequence-specific oligo-nucleotide (SSO) method (Luminex LABType SSO OneLambda, Canoga Park, California) that did not show any

anomalous reaction pattern. After SSO typing, as requiredby our typing strategy, we performed a sequencing analysis(SBT AQ3) to solve all the different ambiguities still present inmost of the HLA loci investigated. The sequencing was per-formed on an ABI 3130XL genetic analyzer and was ana-lyzed with SBT Engine software (IPD-IMGT/HLA 3.21.0).Analyzing the HLA-C locus by SBT (GenDX excelleratorHLA-C core kit AQ4) we observed an atypical nucleotidesequence, due to the presence of a single-nucleotide differ-ence (C/G) in exon 2 at position 286, suggesting the possi-ble existence of a new allele in the complete typing of theindividual. In order to discriminate which of the 2 HLA-Calleles might be carrying the new polymorphism, we usedthe GSSP C21 (GenDX excellerator HLA-C extended kit)

FIGURE 1 Alignment of the exon

AQ5

2 sequence of HLA-C*07:555 with the sequence of HLA-C*07:01:01:01. Dashes (–) indicate nucleotide identity withthe HLA-C*07:01:01:01 sequence, the difference between the 2 alleles in codon 72 is indicated by an arrow.

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Received: 21 December 2016 Revised: 30 December 2016 Accepted: 3 January 2017

DOI 10.1111/tan.12964

HLA 2017; • • wileyonlinelibrary.com/journal/tan © 2017 John Wiley & Sons A/S.Published by John Wiley & Sons Ltd

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Journal Code Article ID Dispatch: 16-JAN-17 CE: Jenifer Merlin BTAN 12964 No. of Pages: 2 ME:

designed for a specific region of allele C*07 allelic group.This further investigation allowed us to confirm the pres-ence of a new HLA-C allele, differing fromC*07:01:01:01 allele for a nucleotide change at position286, varying the specific codon from CAC to CAG,responsible for an amino acid change from glutamine toglutamic acid (Figure 1). The sequence of the new C*07allele was submitted to GenBank and IPD-IMGT/HLADatabase and assigned the submission numbers KX890095and HWS10026927, respectively. The name C*07:555 hasbeen officially assigned by World Health Organization(WHO) Nomenclature Committee in November 2016. Thisfollows the agreed policy that subject to the conditionsstated in the most recent Nomenclature Report,1 nameswill be assigned to the new sequences as they are identi-fied. List of such new names will be published in the fol-lowing WHO Nomenclature Report. The final typing ofthe donor was as follows: A*02:01; B*08:01, *51:01P;

C*01:02P, *07:555; DRB1*07:01, *11:01; DRB3*02:02;DRB4*01:03; DQA1*02:01, *05:01P; DQB1*02:01P,*03:01P; DPB1*04:01P.

Conflict of interest

The authors have declared no conflicting interests.

REFERENCE

1. Robinson J, Halliwell JA, Hayhurst JD, Flicek P, Parham P, Marsh SGE.The IPD and IMGT/HLA database: allele variant databases. Nucleic AcidsRes. 2015;43:D423-D431.

How to cite this article: Condello R, Battarra M,Testi M, Testa G and Andreani M. Identification ofthe new C*07:555 allele in an unrelated donor forHSCT, HLA, 2017. doi: 10.1111/tan.12964

2 CONDELLO ET AL.

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IMPORTANT NOTE: Please mark your corrections and answers to these queries directly onto the proof at the relevant place. DO NOT mark your

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