408 Persoonia–Volume43,2019

Talaromyces guatemalensis

409Fungal Planet description sheets

© 2019 Naturalis Biodiversity Center & Westerdijk Fungal Biodiversity Institute

FungalPlanet1035–18December2019

Talaromyces guatemalensis A.Nováková,Svec,F.Sklenar,Kubátová&M.Kolařík, sp. nov. Etymology. Named according to the geographical origin of investigated isolates.

Classification—Trichocomaceae, Eurotiales, Eurotiomy-cetes.

Onmalt extract agar (MEA).Conidiophores biverticillate-symmetrical,stipesmooth,144–151×4.7µm,underpenicilluswithspathulatewidening.Metulaecylindrical,smooth,4–5in compactterminalwhorls,17.6×3.9–4.7µm.Phialides ampulli-form-acerose,3–4inwhorls,12–14.4×3.6(–4.8)µm.Conidia elliptical,rough˗walled,3.1–3.9×2.3µm. Culture characteristics—(in the dark, 25°C after 7d):Coloniesonmaltextractagar(MEA)synnematal,colonymar-ginsbrilliantyellow(ISCC–NBSNo.83)withverylowacerosesynnematacolouredalsobrilliantyellow,colonycentre(15mmdiam)withdistinctacerosesynnemataupto6–8mmhigh,turntogreyisholivegreen(No.127),somewithyellowishtops,noexudate,nosolublepigment,reversevividyellow(No.82)tostrongorangeyellow(No.68).ColoniesonCzapekyeastau-tolysateagar(CYA)synnematal,synnematashort,brillianttomoderateyellow(No.83,87),1mmto2–3mmhighincolonycentreandgreyishgreenishyellow(No.105)tolightgreyisholive(No.109)with4–5mmyellowishgrey(No.93)centreand2 mm submerged colony margin, soluble pigment vivid red-dishorange(No.34)todeepreddishorange(No.38),reversedeepreddishbrown(No.44)withpalermargins.ColoniesonCzapek-Doxagar(CZA)synnematalwithlowsynnemata,grey-ishgreenishyellow(No.105)onmargintogreyisholivegreen(No.127)withpalertopsinthecentre,noexudate,delicatetostrong(5–8mmring)solublepigmentvividreddishorange(No.34)missinginanycolonies,reversedarkreddishbrown(No.44).ColoniesonCzapekyeastautolysateagarwith20%sucrose(CY20S)plane,lowlanosewith1–5mmsubmerged

Colour illustrations. Pools in theCahabòn river inSemucChampey,Guatemala.Seven-day-oldcoloniesonCYA,MEA,CZAandCREA(columnleft);conidiophoreandconidia(lightmicroscopy)andSEMphotographyofconidia;columnsontheright–coloniesofCCF6215andCCF6214isolatesonbeer-wortagar(7d),CZAandCREA(14d).Scalebars=20,10and5µm.

Phylogenetic tree depicting the position of Talaromyces guatemalensis within the section Trachyspermi.ThetreewasinferredinIQ-TREEv.1.6.5fromaconcatenatedalignmentofDNAsequencesfromtheITSregionofrDNAandpartialgenesofβ-tubulin,calmodulinandRPB2.ModelsofevolutionforeachpartitionwerecalculatedinjModeltestv.2.1.7.

AlenaNováková,LaboratoryofFungalGeneticsandMetabolism,InstituteofMicrobiologyoftheCAS,v.v.i.,Vídeňská1083,14220Prague4,CzechRepublic;e˗mail:ANmicrofungi@seznam.cz

KarelŠvec,FrantišekSklenář&MiroslavKolařík,LaboratoryofFungalGeneticsandMetabolism,InstituteofMicrobiologyoftheCAS,v.v.i.,Vídeňská1083,14220Prague4andDepartmentofBotany,FacultyofScience,CharlesUniversity,Benátská2,12801Prague2,CzechRepublic;

e˗mail:karel.svec@biomed.cas.cz,FandaSklenar@seznam.cz&miroslavkolarik@seznam.czAlenaKubátová,DepartmentofBotany,FacultyofScience,CharlesUniversity,Benátská2,12801Prague2,CzechRepublic;

e˗mail:kubatova@natur.cuni.cz

margins, acerose synnemataproduction sporadic, 1–2mmhigh,paleyellow(No.89),moreabundantin10–12mmcentre,whereare5–6mmhigh,greyisholive(No.110)withpaleyel-low(No.89)tops,lanosepartsofcolonieslightbrown(No.57)tobrownishorange(No.54),noexudate,nosolublepigment,reverselightbrown(No.57)tobrownishorange(No.54)withdarker3–5mmcentreandpaleorangeyellow(No.73)marginsand strongorange (No. 50) borderlinebetween submergedandlanoseparts.Coloniesoncreatinesucroseagar(CREA)plane, velutinous˗granulose turning distinctly synnematous,sporulationlightgreyisholive(No.109),noexudate,noacidproduction,butpureproductionofdeepreddishorange(No.38)solublepigmentinsomecolonies,reversedeepreddishbrown(No.44).Nogrowthat37°C.

Typus.guatemala,SemucChampeyontheCahabònriver,undersmalloverhangoflimestone,exsoilfromtropicalforestusingthesoildilutionplatemethod,2017,K. Švec(holotypePRM952195,cultureex˗typeCCF6215,ITS, tub2, CaM and RPB2sequencesGenBankMN322789,MN329687, MN329688andMN329689,MycoBankMB832313).

Additional material examined. guatemala, Semuc Champey on the Ca-habònriver,undersmalloverhangoflimestone,exsoilfromtropicalforestusingthesoildilutionplatemethod,2017,K. Švec,GUA2˗1(=CCF6214),GUA2˗3andGUA2˗4.

Notes—IsolateGUA2˗1(CCF6214,PRM952196)isphe-notypicallydifferent(brilliantyellowtobrilliantgreenishyellowpigmentationofcoloniesonBWAandMEA,synnemata80–100µmhigh,noorpureproductionofsolublepigmentonCYAandCZA,bigreddishdropletsonCZAin14d(onthecontraryintheex-typeisolate,deepreddishorange(No.41)solublepig-ment with distinct abundant occurrence in colony environs and reddishblack(No.24reversewerefound),verygoodgrowthandsporulationaswellasmassiveacidproductiononCREA).However,themolecularanalysesofalltestedgenes(ITS,tub2, CaM and RPB2)revealedthemtobeidentical.

Talaromyces atroroseus

Talaromyces erythromellis

Talaromyces guatemalensis

Talaromyces austrocalifornicus

Talaromyces albobiverticillius

Talaromyces udagawae

Talaromyces convolutus

Talaromyces minioluteus

Talaromyces trachyspermus

Talaromyces purpureogenus

Talaromyces assiutensis

Talaromyces solicola

Talaromyces diversus

Talaromyces ucrainicus

100

100

100

98

90

98

100

100

100

100

65

Tree scale: 0.01