Table-Top System for Laser Induced Forward Transfer 2011€¦ · • Transferable materials:...
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Fraunhofer Institute for Laser Technology ILT, www.ilt.fraunhofer.de DQS certified by DIN EN ISO 9001, Reg.-No.: DE-69572-01 Subject to alterations in specifications and other technical information. 05/2011. 2 1 1 Table-top system for LIFT: Protoprinter. 2 Protein microarray produced by LIFT. Task Biomedical engineering applications, used to manufacture microarrays or in vitro assays, involve the transfer of valuable, high-viscous or solid materials. State of the art processes such as inkjet printing cannot handle these materials satisfactory. In particular, a transfer of high viscous materials in small volumes (picoliter) or solid materials is challenging. Thus a new techno- logy, that allows the handling of these materials, is needed. Method A pulsed laser source is used to evaporate a sacrificial absorp- tion layer, thereby transferring a material from a transfer slide to a receiver surface. This enables the transfer of high-viscous and solid materials without damage, achieving a spot size of down to 10 μm and with minimal material loss. Even sensitive objects such as cells can be accurately positioned on any given surface. A commercially available plotter is adapted for this purpose. Performance Data • Three-axis positioning system based on the GeSiM Nano-Plotter NP 2.1 • Microchip Laser (355 nm) • Laser scanning module, 70 mm x 70 mm field of view • Resolution X/Y: 2 μm, Z: 10 μm • Working area X/Y/Z: 200 x 165 x 40 mm • Transferable materials: biopolymers (e. g. collagen, alginate, BD MatrigelTM, etc.), living cells Result In collaboration with GeSiM GmbH, a scanner and a minia- turized solid-state laser were added to a compact positioning system, the Protoprinter. This setup supports end-to-end processing of carriers for transfer materials. The donor film and the substrate to be coated can be positioned relative to one another, thus allowing free formed pattern to be printed. Applications The developed table-top Protoprinter system for high-viscous and solid materials supports rapid transfer of proteins and DNA or it can be used in the manufacture of analytical microarrays or in vitro assays. The portable setup allows a production in standard environmental conditions. Flexible component holder and interchangeable material carriers also pave the way for transferring solid materials and new formulations of bioactive materials onto medical engineering components. Consequently the LIFT process also adds up to an economic solution for small-scale applications. Contacts Dipl.-Biol. Dominik Riester Phone +49 241 8906-529 [email protected] Dr. Arnold Gillner Phone +49 241 8906-148 [email protected] TABLE-TOP SYSTEM FOR LASER INDUCED FORWARD TRANSFER: PROTOPRINTER
Transcript of Table-Top System for Laser Induced Forward Transfer 2011€¦ · • Transferable materials:...
Fraunhofer Institute for Laser Technology ILT, www.ilt.fraunhofer.de
DQS certified by DIN EN ISO 9001, Reg.-No.: DE-69572-01
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1 Table-top system for LIFT: Protoprinter.
2 Protein microarray produced by LIFT.
Task
Biomedical engineering applications, used to manufacture
microarrays or in vitro assays, involve the transfer of valuable,
high-viscous or solid materials. State of the art processes such
as inkjet printing cannot handle these materials satisfactory. In
particular, a transfer of high viscous materials in small volumes
(picoliter) or solid materials is challenging. Thus a new techno-
logy, that allows the handling of these materials, is needed.
Method
A pulsed laser source is used to evaporate a sacrificial absorp-
tion layer, thereby transferring a material from a transfer slide
to a receiver surface. This enables the transfer of high-viscous
and solid materials without damage, achieving a spot size of
down to 10 μm and with minimal material loss. Even sensitive
objects such as cells can be accurately positioned on any given
surface. A commercially available plotter is adapted for this
purpose.
Performance Data
• Three-axis positioning system based
on the GeSiM Nano-Plotter NP 2.1
• Microchip Laser (355 nm)
• Laser scanning module, 70 mm x 70 mm field of view
• Resolution X/Y: 2 μm, Z: 10 μm
• Working area X/Y/Z: 200 x 165 x 40 mm
• Transferable materials: biopolymers (e. g. collagen,
alginate, BD MatrigelTM, etc.), living cells
Result
In collaboration with GeSiM GmbH, a scanner and a minia-
turized solid-state laser were added to a compact positioning
system, the Protoprinter. This setup supports end-to-end
processing of carriers for transfer materials. The donor film
and the substrate to be coated can be positioned relative to
one another, thus allowing free formed pattern to be printed.
Applications
The developed table-top Protoprinter system for high-viscous
and solid materials supports rapid transfer of proteins and
DNA or it can be used in the manufacture of analytical
microarrays or in vitro assays. The portable setup allows a
production in standard environmental conditions. Flexible
component holder and interchangeable material carriers
also pave the way for transferring solid materials and new
formulations of bioactive materials onto medical engineering
components. Consequently the LIFT process also adds up to an
economic solution for small-scale applications.
Contacts
Dipl.-Biol. Dominik Riester
Phone +49 241 8906-529
Dr. Arnold Gillner
Phone +49 241 8906-148
Table-Top sysTem for laser induced forward Transfer: proToprinTer
