Table S1: Number of mice used for each experiment RBP4 ...Shen J. ! 1! SUPPLEMENTARY FIGURE LEGENDS...
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Table S1: Number of mice used for each experiment RBP4 +/+ RBP4 −/− RBP4 +/+ RBP4 −/− RBP4 +/+ RBP4 +/− RBP4 −/− RBP4 +/+ RBP4 −/− RBP4 +/+ RBP4 −/− Fig. 2 RT-PCR for tissue specificity - - - - 2 2 2 - - - - Fig. 2 Liver Rbp4 mRNA by northern blot - - - - 2 - 2 - - - - Fig. 2 Serum Rbp4 by western blot - - - - 2 - 2 - - - - Fig. 10 Serum retinol - - 4 4 - - - - - 3 4 Fig. 10 Liver retinol - - 4 4 - - - - - 3 4 Fig. 11 Liver Rbp4 mRNA by qRT-PCR 4 - - - 4 - - 4 - - - Fig. 11 Serum Rbp4 by western blot 4 - - - 4 - - 4 - - - RBP4 +/+ RBP4 −/− RBP4 +/+ RBP4 −/− RBP4 +/+ RBP4 −/−- RBP4 +/+ RBP4 −/−- Fig. 3 & 4 Electroretinogram 6 6 6 6 6 6 6 5 Fig. 5 Retinal histology - - - - - - 6 7 Fig. 6 Measurement of retinal properties - - - - - - 6 6* Fig. 7 Transmission electron microscopy - - - - - - 2 2 Fig. 8 Funduscopic examination 6 7 6 7 6 7 6 7 Fig. 9 Genotyping of the Crb1 locus - - - - - - 6 7 Fig. No. Fig. No. 30 weeks 12 weeks 40 weeks * One of the Rbp4 −/− mice exhibiting serious collapse of the structure of the peripheral retina ( shown in Fig. 5I) was excluded from the analysis. 7 weeks 14 weeks 24 weeks 8 weeks 6 weeks 24 weeks
Transcript of Table S1: Number of mice used for each experiment RBP4 ...Shen J. ! 1! SUPPLEMENTARY FIGURE LEGENDS...
Table S1: Number of mice used for each experiment
RBP4+/+ RBP4−/− RBP4+/+ RBP4−/− RBP4+/+ RBP4+/− RBP4−/− RBP4+/+ RBP4−/− RBP4+/+ RBP4−/−
Fig. 2 RT-PCR for tissue specificity - - - - 2 2 2 - - - -Fig. 2 Liver Rbp4 mRNA by northern blot - - - - 2 - 2 - - - -Fig. 2 Serum Rbp4 by western blot - - - - 2 - 2 - - - -Fig. 10 Serum retinol - - 4 4 - - - - - 3 4Fig. 10 Liver retinol - - 4 4 - - - - - 3 4Fig. 11 Liver Rbp4 mRNA by qRT-PCR 4 - - - 4 - - 4 - - -Fig. 11 Serum Rbp4 by western blot 4 - - - 4 - - 4 - - -
RBP4+/+ RBP4−/− RBP4+/+ RBP4−/− RBP4+/+ RBP4−/−- RBP4+/+ RBP4−/−-
Fig. 3 & 4 Electroretinogram 6 6 6 6 6 6 6 5Fig. 5 Retinal histology - - - - - - 6 7Fig. 6 Measurement of retinal properties - - - - - - 6 6*Fig. 7 Transmission electron microscopy - - - - - - 2 2Fig. 8 Funduscopic examination 6 7 6 7 6 7 6 7Fig. 9 Genotyping of the Crb1 locus - - - - - - 6 7
Fig. No.
Fig. No.
30 weeks12 weeks
40 weeks
* One of the Rbp4−/− mice exhibiting serious collapse of the structure of the peripheral retina ( shown in Fig. 5I) wasexcluded from the analysis.
7 weeks 14 weeks 24 weeks
8 weeks6 weeks 24 weeks
Shen J.
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SUPPLEMENTARY FIGURE LEGENDS
Figure S1. Selection of targeted ES cell clones. A 5.1-kb band was detected with a
neo probe in targeted ES cell clones in Southern blot analysis after digestion with
XhoI/SalI and an 8.8-kb band after digestion with AgeI/HpaI (upper panel). These
clones gave an 8.2- and 10.1-kb bands after digestion with MunI and 7.5- and 9.3-kb
bands after digestion with SacII/HpaI when we used a 5′ or 3′ probe, respectively
(lower panel).
Figure S2. Examples of the entire retinas of Rbp4−/− mice. The scale bar: 500µm.
Figure S1
XhoI SalI
8.2 kb
Targeted allele
AgeI HpaI
8.8 kb TT
2
19
83
87
XhoI/SalI AgeI/HpaI
8.8 kb 5.1 kb
73
19
83
87
73
TT2
1 PGK-neo pA 3 4 5
neo
prob
e
2 4 5 3 1 6
MunI MunI
5.1 kb
MunI 10.1 kb
10.1 kb
19
83
87
TT2
8.2 kb
5’pr
obe
Mun1
SacII HpaI
SacII
MunI
9.3 kb
9.3 kb
19
83
87
TT2
7.5 kb
SacII/HpaI
Wild allele
7.5 kb
3’ p
robe
Shen J et al
Figure S2 Shen J et al
B
C
A
