SWIFS Toxicology Laboratory Training Manual v2.0 (02.22.2008) - Re Formatted 56 Pages

8/2/2019 SWIFS Toxicology Laboratory Training Manual v2.0 (02.22.2008) - Re Formatted 56 Pages

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Dallas County Southwestern Institute of Forensic Sciences

TOXICOLOGY LABORATORY

TRAINING MANUAL Version 2.0

Authorized by: Elizabeth L. Todd, Ph.D., Chief Forensic Chemistry

Chris Heartsill, Supervisor Toxicology Laboratory

Chris Heartsill, Acting Quality Manager

Effective date: January 22, 2008


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Dallas County Institute of Forensic Sciences

Toxicology Training Manual

Summary of Changes from Previous Manual Version

Previous Version: Toxicology Training Manual, version 1.0Current Version: Toxicology Training Manual, version 2.x

1. Update training form consistent with practice that completion of training isacknowledged by the trainee, the supervisor and the section chief.

2. Sections were added to document running initial assays under the direction of anexperienced analyst.

3. Correction of various typographical and grammatical errors.4. Various non-substantive changes in wording and format to improve clarity.5. Conversion of the hardcopy manual to an electronic form.

Dallas County Institute of Forensic Sciences 1 Summary of Changes

Toxicology Training Manual Version 2.0

This is a non-controlled copy of a controlled document


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Toxicology Training Manual Version 2.x

Revisions and Corrections

Effective

Date

Description Authorized

by

Dallas County Institute of Forensic Sciences 1 Corrections and Revisions




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TRAINING MANUAL - OVERVIEW

The Institutes employee training program is established in Training Programs found in the

Quality Manual. All new employees and newly assigned employees must complete anapplicable training program which is documented using training checklists. The training

checklist should be completed by the trainee and trainer throughout the training process with

input and guidance of the Supervisor and/or Section Chief. The checklist and associated

instrument documents will be submitted to the Quality Manager.

I. Toxicology Chemist II

The goal of the Toxicology Chemist II training program is as follows:

Establish proficiency in all types of analyses and instrumental techniques performed by theLaboratory

Develop an understanding of the analytical techniques used Develop proficiency in the basic operation, troubleshooting and repair of instrumentation Select appropriate specimen for analysis Appropriately apply analytical procedures for the accurate analysis of specimen routinely

submitted to the Laboratory

Produce a properly documented case file Effectively evaluate instrumental output and results of standard and quality control samples Effectively communicate analytical procedures and results of analysis Participate in technical case review Seek supervisory assistance in an appropriate mannerThe Toxicology Procedure Manual including training notes and selected reference material form

the basis of the training program. The analyst is referred to the Section Administrative Manual

for information regarding the Sections procedure for receiving off-site training.

The training process for the Toxicology Chemist II is divided into three stages:

Stage 1 - Basic Training

Stage 1 training includes developing proficiency in a core set of analysis that all Toxicology

Chemists are expected to perform and completing required technical training regarding

instrumentation, specimen preparation, and postmortem toxicology. The core set of analyseslearned in this stage of training includes the alkaline and acid/neutral drug screens, alcohols and

acetone, carboxyhemoglobin, and electrolytes assays. Proficiency with this core set of analyses

must be demonstrated prior to completion of the probationary period (6 months) and is usually

completed within two to three months depending upon the experience of the employee. In

addition to these analyses, each toxicologist must complete required technical training

encompassing instrument theory and operation, instrument maintenance, basic principles in

postmortem toxicology, and specimen selection and preparation. At the conclusion of Stage 1

Dallas County Institute of Forensic Sciences 1 Training Overview




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training, each employee demonstrates competency through a written exam and/or moot court.

Refer to the Stage 1 Checklist for details of this portion of the training program.

Stage 2

In Stage 2 training, the Toxicology Chemist II is trained in screening procedures including theELISA (enzyme linked immuno-sorbent assay) and the FPIA (fluorescence polarization

immunoassay). They also receive training in more advanced GC/MS data analysis and data

acquisition for both selected ion monitoring and full scan modes. Completion of Stage 2 should

be completed within nine months of the employees start date and will begin immediately after

completion of Stage 1. Upon successful completion of Stage 2, the Toxicology Chemist II will

be proficient in performing and evaluating most types of analyses performed by the Laboratory

and will begin participating as a technical/peer reviewer for the techniques in which they have

demonstrated competency. Refer to the Stage 2 Checklist for details of this portion of the

training program.

Stage 3

Stage 3 encompasses the ongoing training that occurs throughout the career of the Toxicology

Chemist II. As applicable, the analyst is trained in other remaining assays offered by the

Toxicology Laboratory. As the level of experience and training continues to expand, the

employee will expand participation in technical/peer review, case management, instrument

troubleshooting and repair, and methods development. The Toxicology Chemist II should have

responsibility for at least one special assay within one year of the start date of employment.

Refer to the Stage 3 Checklist for details of this portion of the training program.

II. Toxicology Chemist III

The goal of the Toxicology Chemist III training program is as follows:

Demonstrate competence at the Toxicology Chemist II level; refer to the Toxicology ChemistII Checklist

Develop proficiency in interpretation of toxicology results including effects on performanceand toxicity

Develop proficiency in applicable principles of pharmacology including extrapolation ofalcohol results

Effectively communicate analytical procedures, results of analysis, and interpretation ofresults

Develop expertise as expert witness Develop skills in advanced instrument troubleshooting and repairThe Toxicology Chemist III position must successfully demonstrate competence beyond the

Toxicology Chemist II level prior to the completion of the probationary period. The initial

training for the Toxicology Chemist III includes required technical training regarding the effects

of alcohol and drugs and how they relate to toxicity, performance, and driving. The Toxicology





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Chemist III also receives training in basic pharmacology and its application in extrapolating

alcohol results. Once trained, the Toxicology Chemist III will have primary responsibility for the

legal alcohol/drug screen program in the Toxicology Laboratory including analytical work,

interpretation of analytical results, and applicable testimony in a court of law. The Toxicology

Chemist III serves as an assistant supervisor providing leadership and oversight of Laboratory

operation as directed by the Supervisor and/or Section Chief.

Training should be completed within one year of hire; competency is evaluated using a written

examination and moot court. Refer to the Toxicology Chemist III Checklist for details of this

training program.

III. Toxicology Supervisor

The Toxicology Supervisor must demonstrate proficiency at the Toxicology Chemist III level.

In addition, the supervisor will receive applicable training in policies and procedures of Dallas

County and other management skills training. Training documentation is retained by the Quality

Manager.



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TRAINING CHECKLIST

Administrative Procedures

Trainee: ____________________________________________ (Print)

As each area of training is covered, that area will be checked off and the trainee and instructor

will initial and date below.

The areas to be covered are as follows:

Initials

Trainee Instructor Date

___ Attend County Orientation _____ _____ ______

___Read IFS Quality Manual _____ _____ ______

___ Read IFS Security Manual _____ _____ ______

___ Read Section Administrative Manual _____ _____ ______

___ Read IFS Code of Ethics and Conduct _____ _____ ______

___ Read Toxicology Procedure Manual _____ _____ ______

Sections 1-19, complete Evidence

Management Checklist

___ Enter time and attendance for one week _____ _____ ______

___ Review operation of the drug safe _____ _____ ______

___ Review peer review and proficiency process _____ _____ ______

___ Review QC processes: balance, pipettors, etc. _____ _____ ______

Dallas County Institute of Forensic Sciences Administrative Training Checklist

Toxicology Training Manual 1 Version 2.0


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Dallas County Institute of Forensic Sciences Administrative Training Checklist

Toxicology Training Manual 2 Version 2.0

TRAINING CHECKLIST

Safety

Trainee: ___________________________________________ (Print)




Initials


___ Read IFS Environmental Health and _____ _____ ______

Safety Manual

___ Attend Safety Training _____ _____ ______

___ Receive Personal Protective Equipment _____ _____ ______

Lab coat

Safety Glasses

___ Safety Equipment Locations _____ _____ ______

Gloves

Eyewash Stations

Emergency Shower

First Aid Kits

Fire Blankets

Emergency Phone Numbers

Fire Extinguishers

Evacuation Rules and Routes

Meet Floor Emergency Wardens

When all the areas have been checked off and initialed, the trainee, supervisor, and section chief

will sign and date this form. This form will then be placed in the analysts training folder that is

kept by the Quality Manager.

Trainee: Date:

Supervisor: Date:

Section Chief: Date:


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TOXICOLOGY TRAINING CHECKLIST

TOXICOLOGY CHEMIST II / LITERATURE AND TECHNICAL REVIEW

Trainee:__________________________________________________(Print)

Instructor:________________________________________________(Print)



The areas to be covered are as follows: InitialsTrainee Instructor Date

____Read Chapter 1, Postmortem Forensic ______ ______ _____Toxicology, in Principles of Forensic Toxicology

____Read Chapter 5, Specimen Preparation, ______ ______ _____in Principles of Forensic Toxicology

____Read Chapter 13 Part 3, Gas Chromatography______ ______ _____in Forensic Toxicology, pp 718 736 in Modern Practice of Gas Chromatography

____Read Chapter 7, Chromatography, in ______ ______ _____

Principles of Forensic Toxicology

____Read Chapter 2 Sections 2.2 and 2.3, ______ ______ _____General Aspects of Gas Chromatography and Gas Chromatography, in Modern Practice

Of Gas Chromatography

____View Capillary Installation, Restek ______ ______ _____

____View Maintaining Your Agilent GC System ______ ______ _____Agilent Technologies

____View Maintaining Your GC/MS System, ______ ______ _____Agilent Technologies

____View GC Inlet Resource Guide, ______ ______ _____

Agilent Technologies

____Read Chapter 2, pp 38-68, Agilent ______ ______ _____

Technologies Hardware Manual 5973N Mass Selective Detector

____Read Chapter 9, Mass Spectrometry, in ______ ______ _____

Principles of Forensic Toxicology

Dallas County Institute of Forensic Sciences 1 Toxicology Chemist II/Literature Review



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Dallas County Institute of Forensic Sciences 2 Toxicology Chemist II/Literature Review


____Read Chapter 1, pp 1-14 in Interpretation ______ ______ _____of Mass Spectra

____Become familiar with Disposition of Toxic ______ ______ _____Drugs and Chemicals in Man, PDR, The Merck Index, and Clarks Isolation and

Identification of Drugs

____Read GC Instrument Procedure and ______ ______ _____

Maintenance

____Read GC/MS Instrument Procedure and ______ ______ _____Maintenance

___GC/MS Operation:___ Auto Tune _____ _____ ______

___ Target Tune _____ _____ ______

___ Sequence Table _____ _____ _________ Generating a Spectrum _____ _____ ______

___ Adding Spectra _____ _____ ______

___ Subtracting Spectra _____ _____ _________ Generating a Library Search _____ _____ ______

___ Generating an Ion Chromatograph _____ _____ ______

___ Air and Water Check (leaks?) _____ _____ ______

___ Criteria for Acceptable Spectra _____ _____ _________ Identification of Unknown Spectra _____ _____ ______

___GC Operation

___ Maintenance (insert, seal, septum) _____ _____ ______

___ Routine operation _____ _____ ______including: calibration

standard

computer operationreprocessing data

____Observe and discuss court testimony ______ ______ _____

____Successfully complete written exam ______ ______ _____


will sign and date this form. This form will then be placed in the analysts training folder that iskept by the Quality Manager.

Trainee: Date:

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 Toxicology Chemist III



TOXICOLOGY CHEMIST III

Trainee:__________________________________________________(Print)

Instructor:________________________________________________(Print)


will initial and date below. The areas to be covered are as follows: Initials


____Read Principles of Forensic Toxicology ______ ______ _____

____Read Medicolegal Aspects of Alcohol ______ ______ _____

____Read Forensic Science Review ______ ______ _____

____Become familiar with Casarett and Doulls ______ ______ _____

Toxicology, Goldfranks Toxicologic Emergencies, and Ellenhorns Medical Toxicology

____Observe and discuss court testimony 6 times ______ ______ _____

______ ______ _____

______ ______ _____

______ ______ _____

______ ______ _____

______ ______ _____

____Complete training in basic pharmacology ______ ______ _____

and extrapolation with written examination

____Attend training on drug/alcohol impairment ______ ______ _____

and toxicity

____Successfully complete moot court ______ ______ _____

____Successfully complete comprehensive ______ ______ _____

written exam

____Court Monitoring Evaluation for initial ______ ______ _____

court testimony




Trainee: Date:

Supervisor: Date:



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Toxicology Chemist Training Summary

Toxicologist____________________________________

Stage I Date Stage II Date Stage III

Alkaline Drug Screen ELISA SIM analysis (

Acid Neutral Drug Screen ADX ANAlcohols and Acetone SIM/Scan Training TH

Electrolytes SIM Method: TH

Carbon Monoxide Peer Review Participation Op

Literature Review Co

Complete Written Exam Eth

Supervisor: Eth

Section Chief: Eth

Va

GH

Metals

Lea

Lith

Toxicology Chemist III Date Ars

Me

Literature Review Volatiles

Observe Court Testimony GC

Basic Pharmacology and Extrapolation Mu

Impairment and Toxicity Training Sta

Moot Court Misc.

Complete Written Exam Ace

Sa

Cy


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Dallas County Institute of Forensic Sciences 1 Evidence Handling



SAMPLE LOG-IN, SAMPLE TRACKING, AND EVIDENCE RECEIPT/RELEASE

Trainee: ____________________________________________ (Print)

Instructor: __________________________________________ (Print)




Initials


___Read Toxicology Procedure Manual _____ _____ _____regarding evidence management

Trainee will spend approximately 1 week with instructor covering all areas of analysis listed

below. Instructor and trainee will initial and date the individual areas when trainee demonstrates

proficiency.

___ Review of Chain of Custody and Proper Seal _____ _____ ______

___ Review of Authorized Personnel _____ _____ ______

___ Receipt of Medical Examiner cases _____ _____ ______

___ Location of paperwork/case folders _____ _____ ______

___ production of case folder

___ evidence receipt paperwork

___ evidence release paperwork

___ administrative billing summary

___ quality control paperwork

___ Receipt of Other cases (H, D, etc.) _____ _____ ______

___ Tox LIMS _____ _____ ______

___ Walk-in Refrigerator assignments _____ _____ ______

___ incoming samples

___ samples being worked

___ samples waiting for long-term storage

___ samples in freezer

___ Filling out the workload logbooks _____ _____ ______


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Dallas County Institute of Forensic Sciences 2 Evidence Handling


___ Filing paperwork in Case Folder _____ _____ ______

___ Supervised Sample Log-in _____ _____ ______

___ 10 medical examiner cases___ 10 outside agency cases

___ Review of referral lab process _____ _____ ______

___ Review of special paperwork/requests _____ _____ ______

___ infectious disease testing

___ metabolic testing

___ histology



Trainee: Date:

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 Acetaminophen by ADX



ACETAMINOPHEN QUANTITATION BY GC

Trainee: ____________________________________________ (Print)





Initials


___Read Acetaminophen Quantitation _____ _____ _____and associated training literature



proficiency.

___ Generation of Sample Work List _____ _____ ______

___ Preparation of Samples for Analysis _____ _____ ______

___ Operation of GC _____ _____ ______

___ Reporting Results and Coding Sheets _____ _____ ______

___ Successful Duplicate + QC Run _____ _____ ______

10 quality control samples

10 repeat samples where available

3 supervised runs

When all the areas have been checked off and initialed, the trainee, supervisor, and section Chief

will sign and date this form. The form will then be placed in the analysts training folder that is


Trainee: __________________________________ Date: _____________

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 Acid/Neutral Screen



ACID-NEUTRAL DRUG SCREEN

Trainee: ____________________________________________ (Print)





Initials


___Read Acid-Neutral Drug Screen _____ _____ _____

and associated training literature



proficiency.



___ GC/MS ChemStation Operation _____ _____ ______

___ Create a Sequence Table___ Save and Run Sequence

___ GC/MS Operation _____ _____ ______





3 supervised runs


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Dallas County Institute of Forensic Sciences 2 Acid/Neutral Screen





Trainee: Date:

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 Acid/Neutral Quantitation



ACID-NEUTRAL DRUG QUANTITATION

Trainee: ____________________________________________ (Print)

Instructor: __________________________________________ (Print)As each area of training is covered, that area will be checked off and the trainee and instructor



Initials


___Re-read Acid-Neutral Drug Screen _____ _____ _____


___Read Selective Ion Monitoring _____ _____ _____



proficiency.


___ Preparation of Standards _____ _____ ______

___ Preparation of Calibrators _____ _____ ______

___ Preparation of Controls _____ _____ ______

___ Preparation of Reagents _____ _____ ______

___ GC / MS Operation _____ _____ ______

___ Instrument Parameters

___ SIM Parameters

___ Data Analysis

___ Successful Quality Control Run _____ _____ ______

10 quality control samples10 repeat samples where available

3 supervised runs


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Dallas County Institute of Forensic Sciences 2 Acid/Neutral Quantitation





Trainee: Date:

Supervisor: Date:



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ALCOHOLS AND ACETONE

Trainee: ____________________________________________ (Print)





Initials


___Read Alcohols and Acetone by Headspace

Gas Chromatography and training literature _____ _____ ______

___ Read Operating Instructions for Headspace

Instruments (Instruments and Computer) _____ _____ ______



proficiency.



___ Headspace Autosampler Operation _____ _____ _____

___ Shimadzu GC Solutions Operation _____ _____ _____

___ Create a Sample List

___ Create a Recalc List

___ Save Sample List

___ Run a Sample List

___ Reprint Report

___ Load a Chromatogram___ Where to Edit the Methods

___ Where to Find Custom Reports

___Interpretation of Chromatograms _____ _____ ______


Dallas County Institute of Forensic Sciences 1 Alcohols and Acetone



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Dallas County Institute of Forensic Sciences 2 Alcohols and Acetone



___ Successful Duplicate Run _____ _____ ______


20 repeat samples where available3 supervised runs




Trainee: Date:

Supervisor: Date:



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ALKALINE DRUG SCREEN

Trainee: ____________________________________________ (Print)


As each area of training is covered, that area will be checked off and the trainee and

instructor will initial and date below.


Initials


___Read Alkaline Drug Screen _____ _____ ______

___ Read ChemStation Software Notes _____ _____ ______

Trainee will spend approximately 1 week with instructor covering all areas of analysis

listed below. Instructor and trainee will initial and date the individual areas when trainee

demonstrates proficiency.

___ Generating Sample Work List _____ _____ ______

___ Quality Control _____ _____ ______

___ Preparing Samples for Analysis _____ _____ ______

___ Gas Chromatography Operation _____ _____ ______

___ ChemStation Operation _____ _____ ______

___ Create a Sequence Table

___ Save and Run Sequence

Dallas County Institute of Forensic Sciences 1 Alkaline Screen



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Dallas County Institute of Forensic Sciences 2 Alkaline Screen


Initials



___ Successful Duplicate + Q.C. Run _____ _____ ______



3 supervised runs

All positive results require confirmation using Gas Chromatography/ Mass Spectrometry

(GC/MS). After completing required reading, listed below, the trainee will spend

approximately 1 week with instructor covering all aspects of GC/MS analysis includingthe following areas.

___ Read Gas Chromatography/ Mass Spectrometry _____ _____ ______

___ 5973 ChemStation Operation _____ _____ ______

___ Create a Sequence Table

___ Save and Run Sequence

___ Run Alkaline Extractions on GC/MS _____ _____ _____

___ Compare Total Ion Chromatogram to GC Run _____ _____ ______

___ Run and Interpret Library Searches _____ _____ ______

When all the areas have been checked off and initialed, the trainee, supervisor, and

section chief will sign and date this form. This form will then be placed in the analysts

training folder that is kept by the Quality Manager.

Trainee: Date:

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 Arsenic by AA



ARSENIC BY AA

Trainee: ____________________________________________ (Print)





Initials


___Read Arsenic by AA _____ _____ _____and associated training literature



proficiency.



___ Operation of Atomic Absorption _____ _____ ______


___ Successful QC Run _____ _____ ______



3 supervised runs



Trainee: Date:

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 Benzodiazepines by GC/MS


TRAINING CHECKLIST

TOXICOLOGY / BENZODIAZEPINES BY GC/MS

Trainee: ____________________________________________ (Print)



will initial and date below.The areas to be covered are as follows: Initials


___Read Selective Ion Monitoring _____ _____ ______

___Read Benzodiazepines by GC/MS

and training literature _____ _____ ______

___Read Handbook of Derivatives for Chromatography _____ _____ ______

Section 4.4.1 page 57-58

___Read Gas Chromatography Derivatization in the _____ _____ ______

Pierce Catalog and Handbook, page T-231-238



proficiency.






___ Derivatization Procedure _____ _____ _____

___ GC / MS Operation _____ _____ ______


___ SIM Parameters

___ Data Analysis




3 supervised runs


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Dallas County Institute of Forensic Sciences 2 Benzodiazepines by GC/MS


When all the areas have been checked off and initialed, the trainee, supervisor, and section Chief

will sign and date this form. The form will then be placed in the analysts training folder that is


Trainee: __________________________________ Date: _____________

Supervisor: Date:



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CARBOXYHEMOGLOBIN

Trainee: ____________________________________________ (Print)





Initials


___Read Carboxyhemoglobin and training literature _____ _____ ______

Trainee will spend approximately 3 days with instructor covering all areas of analysis listed


proficiency.

___ Nova Biomedical Critical Care XPress Operation _____ _____ ______

___ Daily Controls _____ _____ ______



___ Reporting Results and Code Sheets _____ _____ ______




3 supervised runs

Dallas County Institute of Forensic Sciences 1 Carboxyhemoglobin

Toxicology Training Manual Version, 2.0


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Dallas County Institute of Forensic Sciences 2 Carboxyhemoglobin

Toxicology Training Manual Version, 2.0




Trainee: Date:

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 Cocaine by GC/MS



COCAINE AND METABOLITES BY GC/MS

Trainee: ____________________________________________ (Print)




The areas to be covered are as follows: Initials



___Read Cocaine and Metabolites by GC/MS _____ _____ ______

and training literature

___Read Gas Chromatography Derivatization in _____ _____ ______Pierce Catalog and Handbook, page T-244



proficiency.







___ GC / MS Operation _____ _____ ______


___ SIM Parameters

___ Data Analysis




3 supervised runs


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Dallas County Institute of Forensic Sciences 2 Cocaine by GC/MS





Trainee: Date:

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 Cyanide



CYANIDE QUANTITATION

Trainee: ____________________________________________ (Print)





Initials


___Read Cyanide Quantitation _____ _____ _____and associated training literature



proficiency.



___ Operation of pH/Ion Specific Electrode Meter _____ _____ ______




3 supervised runs




Trainee: Date:

Supervisor: Date:



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ELECTROLYTES

Trainee: ____________________________________________ (Print)





Initials


___Read Electrolytes By NOVA Biomedical Critical Care XPress _____ _____ ______

Trainee will spend approximately 3 days with instructor covering all areas of analysis listed


proficiency.

___ NOVA Biomedical CCX Operation / Maintenance _____ _____ _____

___ Quality Control _____ _____ ______



___ Calculation Rules _____ _____ ______




3 supervised runs

Dallas County Institute of Forensic Sciences 1 Electrolytes



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Dallas County Institute of Forensic Sciences 2 Electrolytes





Trainee: Date:

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 ELISA



DRUG SCREENING BY ELISA

Trainee: ____________________________________________ (Print)





Initials


___Read ELISA _____ _____ _____and associated training literature



proficiency.



___ Operation of Tecan Miniprep and Reader _____ _____ ______



3 repeat full runs where available

3 supervised runs




Trainee: Date:

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 Ethylene Glycol



ETHYLENE GLYCOL

Trainee: ____________________________________________ (Print)





Initials


___Read Ethylene Glycol Quantitation _____ _____ _____and associated training literature



proficiency.



___ Operation of GC/MS _____ _____ _________ Instrument Parameters

___ SIM Parameters

___ Data Analysis




repeat samples where available

3 supervised runs


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Dallas County Institute of Forensic Sciences 2 Ethylene Glycol





Trainee: Date:

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 GHB

Toxicology Training Manaual Version 2.0


GAMMA HYDROXYBUTYRATE

Trainee: ____________________________________________ (Print)





Initials


___Read Gamma Hydroxybutyrate _____ _____ _____and associated training literature



proficiency.




___ SIM Parameters

___ Data Analysis





3 supervised runs


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Dallas County Institute of Forensic Sciences 2 GHB

Toxicology Training Manaual Version 2.0




Trainee: Date:

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 Lead by AA



LEAD BY GRAPHITE FURNACE AA

Trainee: ____________________________________________ (Print)





Initials


___Read Lead by Graphite Furnace AA _____ _____ _____and associated training literature



proficiency.







3 supervised runs




Trainee: Date:

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 Lithium by AA



LITHIUM BY AA

Trainee: ____________________________________________ (Print)





Initials


___Read Lithium by AA _____ _____ _____and associated training literature



proficiency.







3 supervised runs




Trainee: Date:

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 Mercury by AA



MERCURY BY AA

Trainee: ____________________________________________ (Print)





Initials


___Read Mercury by AA _____ _____ _____and associated training literature



proficiency.







3 supervised runs




Trainee: Date:

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 Methocarbamol Quantitation



METHOCARBAMOL by GCMS QUANTITATION

Trainee: ____________________________________________ (Print)

Instructor: __________________________________________ (Print)As each area of training is covered, that area will be checked off and the trainee and instructor



Initials


___Re-read Methocarbamol _____ _____ _____


___Read Selective Ion Monitoring _____ _____ _____



proficiency.






___ GC / MS Operation _____ _____ ______


___ SIM Parameters

___ Data Analysis


10 quality control samples3 supervised runs


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Dallas County Institute of Forensic Sciences 2 Methocarbamol Quantitation





Trainee: Date:

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 Opiates by GC/MS



OPIATES BY GC/MS

Trainee: ____________________________________________ (Print)







___Read Opiate by GC/MS and training literature _____ _____ ______


Section 4.4.1 page 57-58


Pierce Catalog and Handbook, page T-231-238



proficiency.







___ GC / MS Operation _____ _____ ______


___ SIM Parameters

___ Data Analysis




3 supervised runs


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Dallas County Institute of Forensic Sciences 2 Opiates by GC/MS





Trainee: Date:

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 Salicylates



SALICYLATES

Trainee: ____________________________________________ (Print)





Initials


___Read Salicylates _____ _____ _____




proficiency.



___ Operation of Fluorescence Spectrophotometer _____ _____ ______





3 supervised runs




Trainee: Date:

Supervisor: Date:



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Dallas County Southwestern Institute of Forensic Sciences


TRAINING MANUAL Version 2.0

Authorized by: Elizabeth L. Todd, Ph.D., Chief Forensic Chemistry

Chris Heartsill, Supervisor Toxicology Laboratory

Chris Heartsill, Acting Quality Manager

Effective date: January 22, 2008


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Dallas County Institute of Forensic Sciences 1 Cannabinoids in Blood



CANNABINOIDS IN BLOOD BY GC/MS

Trainee: ____________________________________________ (Print)


As each area of training is covered, that area will be checked off and the trainee and instructorwill initial and date below.




___Read Cannabinoids in Blood by GC/MS _____ _____ ______


Section 4.4.1, pages 57-58


Pierce Catalog and Handbook, page T-231-238Trainee will spend approximately 1 week with instructor covering all areas of analysis listed


proficiency.







___ GC / MS Operation _____ _____ ______


___ SIM Parameters

___ Data Analysis




3 supervised runs


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Dallas County Institute of Forensic Sciences 2 Cannabinoids in Blood





Trainee: Date:

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 Carboxy-THC in Urine



CARBOXY-THC IN URINE BY GC/MS

Trainee: ____________________________________________ (Print)


As each area of training is covered, that area will be checked off and the trainee and instructorwill initial and date below.




___Read Carboxy -THC in Urine by GC/MS _____ _____ ______


Section 4.4.1, pages 57-58


Pierce Catalog and Handbook, page T-231-238Trainee will spend approximately 1 week with instructor covering all areas of analysis listed


proficiency.







___ GC / MS Operation _____ _____ ______


___ SIM Parameters

___ Data Analysis




3 supervised runs


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Dallas County Institute of Forensic Sciences 2 Carboxy-THC in Urine





Trainee: Date:

Supervisor: Date:



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Dallas County Institute of Forensic Sciences 1 Valproic Acid, Ethosuximide, Ethchlorvynol



VALPROIC ACID, ETHOSUXIMIDE, ETHCHLORVYNOL

Trainee: ____________________________________________ (Print)





Initials


___Read Valproic Acid, Ethosuximide, Ethchlorvynol _____ _____ _____and associated training literature



proficiency.




___ SIM Parameters

___ Data Analysis


___ Successful QC Run (only one analyte required) _____ _____ ______



3 supervised runs


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Dallas County Institute of Forensic Sciences 2 Valproic Acid, Ethosuximide, Ethchlorvynol





Trainee: Date:

Supervisor: Date:



54/56

Dallas County Institute of Forensic Sciences 1 Volatiles by GC/MS



VOLATILES BY GC/MS

Trainee: ____________________________________________ (Print)





Initials


___Read Volatiles by GC/MS _____ _____ _____and associated training literature



proficiency.



___ Operation of GC/MS _____ _____ ______




3 supervised runs




Trainee: Date:

Supervisor: Date:



55/56

Dallas County Institute of Forensic Sciences 1 Volatiles by Headspace GC

Toxicology Training Checklist Version 2.0


VOLATILES BY HEADSPACE GC

Trainee: ____________________________________________ (Print)





Initials


___Read Volatiles by Headspace GC _____ _____ _____and associated training literature



proficiency.



___ Operation of Headspace GC _____ _____ ______




3 supervised runs




Trainee: Date:

Supervisor: Date:



56/56


VOLATILES BY MULTIPLE HEADSPACE

Trainee: ____________________________________________ (Print)





Initials


___Read Volatiles by Multiple Headspace _____ _____ _____and associated training literature



proficiency.



___ Operation of Headspace GC _____ _____ ______




3 supervised runs




Trainee: Date:

SWIFS Toxicology Laboratory Training Manual v2.0 (02.22.2008) - Re Formatted 56 Pages

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Transcript of SWIFS Toxicology Laboratory Training Manual v2.0 (02.22.2008) - Re Formatted 56 Pages