ESF provides the COST Officethrough a European Commission contract

COST is supported by the EU Framework Programme

Sustainable control of Grapevine Trunk Diseases FA 1303 - ManaGTDs Start date: 22/10/2013 End date: 21/10/2017 Year: 2 FONTAINE Florence Chair University of Reims Champagne-Ardenne - FRANCE

Website http://managtd.eu/

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February 2015

Working Group 1 and WorkingGroup 2 meeting - Budapest,

Hungary: 26-27/02/2015(/en/news/working-group-1-and-working-group-2-meeting-budapest-hungary-26-27-02-2015.html)

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News

Working Group 1 and Working Group 2 meeting - Budapest,Hungary: 26-27/02/2015

Local organizers: Dr Erzsebet Karaffa associate professor University of DebrecenFaculty of Agricultural and Food Science and Environmental Management Institute ofFood Science H-4032 DEBRECEN, Böszörményi út 138., Hungary Tel.: +36 52 508 444 /88067 ext E-mail: karaffa@agr.unideb.hu, karaffaem@yahoo.co.ukhttp://portal.agr.unideb.hu ...

(/en/news/working-group-1-and-working-group-2-meeting-budapest-hungary-26-27-02-2015.html)

Call for proposals: "Search for applied solutions to controlgrapevine trunk diseases" - Funded by Jas HENNESSY & CoCall time schedule • December, 15th, 2014: call announcement;• February, 15th, 2015: deadline for submittingpre-proposals;• February, 15th - March, 15th 2015: evaluation of the pre-proposals;• March 2015: communicationto the applicants of the evaluation results and rating (A,B or C);• May , 15th 2015: deadline for submitting fullproposals;• May...(/en/news/call-for-proposals-search-for-applied-solutions-to-control-grapevine-trunk-diseases-funded-by-jas-hennessy-amp-co.html)

Disease cyclesDavid Gamaje and Philippe Larignon prepared a document including the graphical representation of the diseasecycle of some grapevine trunk disease agents. The document is attached below....(/en/news/disease-cycles.html)

Other Working Group meetingsWG3 (Host-pathogen and fungus-fungus competitive interactions) meetingAthens, Greece – 14-15 May 2015LocalOrganizers: Pr Epameinondas Paplomatas Agricultural University of Athenshttp://www.aua.gr/inde...(/en/news/other-working-group-meetings.html)

(http://ec.europa.eu/) COSTis supported by the EU RTDFramework Programme

(http://www.esf.org/) ESFprovides the COST Officethrough a European (http://www.cost.eu/) manaGTD is

supported by COST

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Tuesday 12th, May

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Athens, 12 -13 May 2015

09:00 Welcome to participants – local organizers 09:30 Florence Fontaine Synthesis of WGs1&2 meeting – Budapest – Feb2015

Plant pathogen interaction Chairman/ moderators Christophe Bertsch/Eckhard Thines

9:45 Jacques Alban France What can we learn from the cutting nodal model on Esca-associated fungi interactions with grapevine?”

10:45 Coffee break 11:00 Ana Cristina Esteves Portugal Enzymes involved in grapevine diseases 12:00 Rebecca Cobos Spain Secreted proteins from Diplodia seriata and their putative role in pathogenesis

13:00 Lunch break

Tusday 12th, May

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•  Pathogen Infection •  Chairman/ moderators Anna Andolfi/ Epaminondas Paplomatas

•  14:30 Dimitris Tsitsigiannis Greece Unravelling the Molecular Networks of the Plant Immune System

•  •  15:30 Eliane Abou-Mansour •  Switzerland Phytotoxins of Botryosphaeria associated with

grapevine •  16:30 Coffee break •  •  17:00-18:30 Round table •  •  19:30 Social dinner

Wednesday, 13th morning

•  •  Plant defense Chairman/ moderators Florence Fontaine/ Sotiris

Tjamos •  09:00 Synthesis -Round table •  11:30 Conclusion Florence Fontaine •  12:00 End of the meeting Florence Fontaine – Workshop in Cognac

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Synthesis of WGs1&2 meeting, Budapest

•  Pathogen characterization and detection James Woodhall

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•  What are the most important GTD species •  Identify the reference isolates and mechanism of access •  Standard test conditions to determine the

aggressiveness of GTD pathogens •  Characterization of the GTD pathogens life cycle !

lecture to Cognac Workshop

The trouble with Phomopsis viticola

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Some uncertainty over which species/sequences

Potential community project/paper – with Pedro Crous?

Collection of grapevine Phomopsis isolates -Josep, Artur, Phillippe, Kalman, David E, David G, Ales, Erzsebet, Ferenc, Stefania & James

Determine which strain/species most important for grapevines

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•  Reference collection of approx. 30 GTD isolates

Purpose: Standards for diagnostics & pathogenicity testing

30 isolates representing the most important species Where variability exists within species exists 2-3 isolates

80C agar plugs/glycerol method Collection distributed to key labs in several countries where -80C All isolates backed up at CBS and given CBS numbers

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Actions: Artur Alves to circulate protocol on -80C storage method

Determining which isolates and providing strains: Black foot pathogens: Cecilia/Anna? Esca/Petri: Josep, David G Cadophora: Josep Phomopsis: Chosen after community activity? Eutypa: David G, James (probably need 2-3 isolates) Botryosphaeria: Artur

Ideally isolates should have been used in publication, DNA barcoded and been tested for pathogenicity

James to circulate draft list to populate

•  Reference collection of approx. 30 GTD isolates

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–  Stipulating which specific conditions (temp, RH) may be too difficult could provide some general recommendations

–  Olivier to look into host material what is available EU wide – maybe 4-5 recommended varieties/clones available.

–  Specialists to review literature for specific methods for: -Esca/Petri & related species – David Gramaje -Illyoncectria – Cecilia/Anna? -Botryosphaeriaceae – Artur Alves -Eutypa- David Gramaje -Other species – James Woodhall

-Report back with recommended method for that group of pathogens

•  Develop standard test conditions to determine the aggression of individual GTD

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•  RT Pathogens Epidemiology L. Guérin-Dubrana and L. Mugnai

COST Objectives •  1- Surveys to assess the presence and

spread of the different GTDs in Europe ! questionnare in process!lecture to Cognac workshop

•  2- Multi site evaluation trials •  3- Alternative tools for spatial distribution

analysis •  4- Databases for prediction models

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•  Multi site evaluation trials

a. To gather information on vineyards with historical data in the country from previous assesments

b. To detect in each country three reference viticulture areas with uniform productive/climatic conditions

c. For each area to select 1 vineyard of a susceptible international cultivar chosen on the base of the 1st level survey

d. In each of the 3 vineyards to select a GEO-REFERENCED plot for GTDs survey

- The same training system –  Possibly of know rootstock

-12-18 yers old - 500 vines at least

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•  The main diseases/symptoms to be surveyed will be: –  Eutypa dieback + Botryosphaeria canker :

•  Eutypa foliar symptom •  Decline •  Dead cordon

–  GLSD (Esca): •  foliar symptom •  measles •  canes/clusters wilting

–  Apoplexy (halph or the whole plant) –  Number of dead plant and presence of other diseases (viruses,

phytoplasmas, heavy pests attacks…)

We will not survey: Phomopsis –  Black foot –  Petri disease (0-7/8 year old)

•  Multi site evaluation trials

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Inventory of microflora and identification of beneficial microorganisms

Patrice Rey

1. Study microbial communities colonizing wood tissues : plants sampled in different countries

2. Sampling of vines over time

3. Interactions between microorganisms

4. Wood tissues : a source of potential biocontrol agents

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To know the pathogens involved in GTDs but also to isolate BCAs specific to certain regions

Standardize the sampling method : " Compare the cultivars =

•  Same international cultivars (Chardonnay, Cabernet) •  Cultivar specifics to certain regions (Furmint to produce tokai,

Mavrud in Bulgaria) " Cultivars use to produce wine / table grapes (South of Italy) ? " Organic or conventional vineyard ? = conventional vineyard "  Same training system (see RT2) ?

Rootstocks = International rootstocks : SO4 ? To select among the first 3 rootstocks

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Foliar GTDs-symptomatic / Asymptomatic : Use vineyards that are surveyed. Uproot grapevine : not a problem, even for healthy plants !

Age of plants: 15- 20 year-old plants

Sampling time : at the veraison (after the symptoms has appeared)

Which part of the plants have we to sample ? = at least 2 samples from the trunk (apparently healthy wood and the most representative necrosis)

Connect quantity of necroses / foliar symptoms / microflora

Study the microflora that colonise the rhizosphere ?

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Standardize the materials and methods :

•  DNA/RNA extraction = verify the quality of DNA/RNA •  Amplifications, primers, etc = to use the same standard

protocol (to be on the website) •  Send the DNA/RNA sequences to one platform

•  Which parts of plants are the more useful to sample : at least 2 samples in the trunk (apparently healthy wood and the most representative necrosis)

1. Study microbial communities colonizing wood tissues : plants sampled in different countries

2. Sampling of vines over time – standardize protocol: uproot vine

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3. Interactions between microorganisms

"  Imaging analyses : to study interactions between the microflora (bacteria / fungi) with tools like DOPE-FISH

"  Cultivable methods to isolate and use the microorganisms

"  Screening of microorganisms: first in Petri dishes (include wood extracts in the agar?) then on plants ?

"  Role of bacteria: they can contribute to the breakdown of wood or remove compounds that may be toxic to decay fungi. They predispose wood to fungal attack (according to the literature).

"  Bacteria + pathogenic fungus, in certain cases = association of wrongdoers?

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4. Wood tissues : a source of potential biocontrol agents

"  Trichoderma strains are used as BCAs (not always isolated from grapevines) :

•  Hungary = many Trichoderma are detected in the wood tissues (3 Trichoderma species from Tokai region, they grow at 5 °C, even at 30 °C).

•  2 Trichoderma species (Remedier) registered in Italy, they are applied on pruning wounds.

•  Esquive in France (to control Eutypa dieback and esca)

" Combine BCAs : For instance: bacteria on the pruning wounds or endophytic bacteria + BCA in the rhizosphere

"  Impact of BCAs on wine quality ?

Guidelines for reimbursement

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Some guidelines •  BEFORE MEETING •  Step 1, accept invitation •  Step 2, reimbursement: yes

•  AFTER MEETING •  Step 3, Travel Reimbursement Request (TRR) •  ASAP

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9/12/14, 3:24 PMInvitation to COST Event

Page 1 sur 3https://e-services.cost.eu/participation/8aa8040e06d1c890596b868bea732d89/

e-COSTe-COSTInvitation to COST EventDr Florence Fontaine

Meeting Details

Meeting ID ECOST-MEETING-FA1303-250914-044181

Title MC and WG 4 meeting

Start Date 2014/09/25

End Date 2014/09/26

Location Poland, Warsaw, Poland

Step1/5 Invitation : Accepted

Step2/5 Reimbursement : Yes

If you decided to NOT submit a claim, please click the 'No' button above.

Step3/5

Travel information and Expenses

Please read carefully the Travel Reimbursement Request (TRR) Rules before completing your expenses.

Travel dates (Door to Door)

Travel Start Date 2014-09-25 Time

Travel End Date 2014-09-26 Time

Meeting Attendance

Check all the attended days according to the signed attendance list.

25 Sep 14

26 Sep 14

Accommodation Expenses i

Number of nights

Applicable Rate per Night EUR 120.00

Reimbursable amount EUR 0.00

Meals Expenses i

Number of meals

Reimbursement procedure

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•  Keep your original invoices !

–  Flight tickets and boarding passes

–  Train tickets –  Travel by car: proof of distance

which shows the traveled route (print-out from Google Maps)

–  Local transport expenses: every tickets

–  Parking tickets

After the meeting : •  Complete your online

reimbursement request (= your invitation on e-COST)

•  Detail each means of transportation (don’t put together the flight price and the airport shuttle price, for example)

•  Upload all your supporting documents

•  Send the signed Travel Reimbursement Request to:

Nathalie RAU – Violaine Béasse University of Reims Champagne Ardennes 9 Boulevard de la Paix CS0005 51724 REIMS - France

1 month to submit your travel reimbursement request on e-COST !

If you have a question, feel free to send it to us before submitting your

claim !

During the meeting : •  Sign the attendance list •  Sign your mission order

GrantHolder contacts •  Nathalie Rau nathalie.rau@univ-reims.fr

•  Violaine Béasse violaine.beasse@univ-reims.fr

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Thank for your attention

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Year 2: Workshop – 4 WGs •  23 & 24 June, 2015 •  Cognac, Fr •  Collaboration with Hennessy

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Paris

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Year 2: Workshop – 4 WGs •  Number of participants: 85 •  Invited speakers: 3, Kendra (USA),

François Halleen (SA), José Ramon (Canada)

•  Duration: 2 days •  Schedule: days 1 & 2, conferences •  day 2-afternoon, trip & cellar •  day 2-night, social diner

•  4 sessions •  35 lectures •  13 posters •  Need information for transfer:

• Angoulème railway • Bordeaux Airport

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Workshop – GTDs: current state and future prospects

Website - platform •  the website managtd.eu hosts a document sharing platform •  If you are interested, please follow this link:

http://managtd.eu/en/members/subscribe/ to register on the website.

•  After our validation, you will be able to download documents in the Members Area.

•  In "Edit my profile" you can also tick in which working group(s) you want to be registered.

•  This part of the website is not open to the public. Only the registered members can access to the documents.The platform is for now divided between the 4 working groups of the COST Action.

•  Email of Sandrine Plaud June 2nd, 2014

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Thank for your attention

Thank Sotiris & Epaminondas for the organization Thank Eliane for the scientific programme

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