Supporting Information for the article: oxidized residues · 3 Figure 3SI: CID-MS2 fragmentation...
Transcript of Supporting Information for the article: oxidized residues · 3 Figure 3SI: CID-MS2 fragmentation...
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Supporting Information for the article:
Tandem mass spectrometry and infrared spectroscopy as a help to identify peptide
oxidized residues
D. Scuderi,*a M. T. Ignasiak a, b, X. Serfaty a, P.de Oliveiraa and C. Houée Levina
Figure 1SI: CID-MS2 fragmentation mass spectrum of (GS-Me)H+ (m/z 322).
100 200 300 400
0
1x107
2x107
229 247
130
176
193
I (a
.u.)
m/z
322
Electronic Supplementary Material (ESI) for Physical Chemistry Chemical Physics.This journal is © the Owner Societies 2015
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Figure 2SI: CID-MS2 fragmentation mass spectrum of GS-Me(O)H+ (m/z 338).
100 200 300 400
0,0
5,0x105
1,0x106
1,5x106
2,0x106
2,5x106
3,0x106
3,5x106
145 256
209
274
308320
I (a
.u.)
m/z
338
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Figure 3SI: CID-MS2 fragmentation mass spectrum of GS-Me(O)2H+ (m/z 354).
100 200 300 400
0
1x105
2x105
3x105
4x105
5x105
6x105
279
225
I(a
.u)
m/z
354
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Figure 4SI: CID-MS2 fragmentation mass spectrum of (GS-Me)H+-CO2 (m/z 278).
100 200 300 400
0,0
2,0x105
4,0x105
6,0x105
8,0x105
1,0x106
130
147
193
261
I (a
.u.)
m/z
278
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Figure 5 SI: CID-MS2 fragmentation mass spectrum of (GS-Me)H+-H2 (m/z 320).
100 200 300 400
0,0
5,0x105
1,0x106
1,5x106
2,0x106
191
308
I(a
.u.)
m/z
320
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Figure 6SI: Mass spectra of non-irradiated (a) and irradiated Trp-Met (b) (irradiation dose 800 Gy).
200 300 400 500 600
0,0
4,0x107
8,0x107
a)
319
336
Inte
nsit
y (
a.u
.)
m/z
200 300 400 500 600
0,0
2,0x107
4,0x107
6,0x107
352
b)
319
336
Inte
nsit
y (
a.u
.)
m/z
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Figure 7SI: Mass spectra of non-irradiated (a) and irradiated Met-Trp (b) (irradiation dose 900
Gy).
250 300 350 400 450 500
0,0
2,0x107
4,0x107
6,0x107
8,0x107
1,0x108
364
336
358
Inte
ns
ity
(a
.u.)
m/z
392
a)
250 300 350 400 450 500
0,0
5,0x106
1,0x107
1,5x107
2,0x107
2,5x107
368
Inte
ns
ity
(a
.u.)
m/z
392364
352
336
b)
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200 300 400 500 600
0,0
2,0x106
4,0x106
6,0x106
8,0x106
319
I (a
.u.)
m/z
336
Figure 8SI: CID-MS2 fragmentation mass spectrum (Trp-Met)H+ (m/z 336).
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200 300 400 500 600
0,0
2,0x106
4,0x106
6,0x106
8,0x106
1,0x107
I(a
.u.)
m/z
352
335
288
271
Figure 9SI: CID-MS2 fragmentation mass spectrum of (Trp-Met)O2H+ (m/z 352).
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Figure 10SI: CID-MS2 fragmentation mass spectrum of (Met-Trp)H+ (m/z 336).
200 300 400 500
0,00
2,50x106
5,00x106
I (a
.u.)
m/z
318
288
271
336205
188
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150 200 250 300 350 400
0
1x106
2x106
3x106
I (a
.u.)
m/z
352
334
316
306
288
270
260
243
221
Figure 11SI: CID-MS2 fragmentation mass spectrum of (Met-Trp)OH+ ( m/z 352).
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Figure 12SI: CID-MS2 fragmentation mass spectrum of (Met-Trp)O2H+ (m/z 368).
150 200 250 300 350 400 450 500
0,0
5,0x105
1,0x106
1,5x106
237
276
286304
322
332350
I(a
.u.)
m/z
368
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Figure 13SI: Mass spectrum of non-irradiated (a) and irradiated (b) Trp (irradiation dose 900 Gy).
50 100 150 200 250 300 350 400 450 500
0,0
2,0x106
4,0x106
409
Inte
ns
ity
(a
.u.)
m/z
205
a)
50 100 150 200 250 300 350 400 450 500
0,0
5,0x106
1,0x107
253269
409
237221
188
Inte
ns
ity
(a
.u.)
m/z
205
b)
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Table 1SI. Most intense peaks in the CID-MS2 fragmentation mass spectra of the products of GS-Me.
.
Compound m/z, z=+1
(GS-Me)H+-CO2
(GS-Me)H+-H2
(GS-Me)H+
278 (parent ion)
261 (-NH3)
193(y2)
147 (y2-H2O-CO)
130 (y2-H2O-CO-NH3 )
320 (parent ion)
308 (-H2O)
191 (y2)
322 (parent ion)
247(b2)
229 (b2-H2O)
193(y2)
176 (y2-NH3)
130 (b1 ou y2-17-H2O-CO)
338(parent ion)
320(-H2O)
274(-CH3SHO)
256(-H2O-CH3SOH)
209 (y2)
145 (y2-CH3SOH)
354 (parent ion)
279 (b2)
225 (y2)
208 (y2- NH3)
130 (b1)
(GS-Me)OH+
(GS-Me)O2H+
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Table 2SI. Most intense peaks observed in the CID-MS2 fragmentation mass spectra of the products of
oxidation of Trp-Met.
Compound m/z, z=+1
(Trp-Met)H+
(Trp-Met)OH+
336 (parent ion)
319 (Trp-Met)H+-NH3
352 (parent ion)
335 (Trp-Met)OH+-NH3
288 (Met-Trp)OH+-CH3SOH
271 (Met-Trp)OH+-NH3-CH3SOH
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Table 3SI. Most intense peaks observed in the CID-MS2 fragmentation mass spectra of the products
of oxidation of Met-Trp.
Compound m/z, z=+1
(Met-Trp)H+
(Met-Trp)OH+
(Met-Trp)O2H+
336 (parent ion)
318 (Met-Trp)H+-H2O
288 (Met-Trp)H+-CH3SH
271 (Met-Trp)H+-NH3
205 (y)
188 (y-NH3)
352 (parent ion)
334 (Met-Trp)OH+-H2O
316 (Met-Trp)OH+-2H2O
306 (Met-Trp)OH+-H2O-CO
288 (Met-Trp)OH+-CH3SOH
270 (Met-Trp)OH+-H2O -CH3SOH
243 (Met-Trp)OH+-H2O -CH3SOH-NH3
221 (y)
368 (parent ion)
350 (Trp-Met)O2H+-H2O
332 (Trp-Met)OH+-2H2O
304 (Trp-Met)O2H+-CH3SOH
286 (Trp-Met)OH+-CH3SOH-H2O
276 (Trp-Met)OH+-CH3SOH-CO
237 yO2
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m/z, z=+1 compound
237 (Trp)O2H+
220 (Trp)O2H+-NH3
219 (Trp)O2H+-H2O
202 (Trp)O2H+-NH3-H2O
192 (Trp)O2H+ -NH3-CO
174 (Trp)O2H+ -NH3-CO-H2O
Table 4SI: Most intense peaks observed in the CID-MS2 fragmentation mass spectrum of ions at m/z
237 and their attribution.
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Scheme 1SI: Schematic representation of y2 fragments for each oxidation product of GS-Me
according the Roepstorff-Fohlman nomenclature.
Y2
2 3
4 5
1
Y2
Y2
Y2
Y2
Y2
b)
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Scheme 2SI: Structures of 5hydroxytrypthophan (a) and oxindolylalanine (b) corresponding to the
addition of an oxygen atom to the tryptophan.
a) b)