Supplementary table 1: List and characteristics of oligonucleotides used in the qPCR and PCR assays

Primer or Probe name Sequence (5’ 3’) Length 5’-Dye Concentration

(nM)

EMBL

Accession # Reference

ccrC Type V (SCCmec V) AB121219 (1)

F_ccrC Type V TGCACTTTATTCGCGCGTTA* 20 100

R_ccrC Type V TTGTTCTTGCTCATGGATTGAGTAC* 25 100

P_ccrC Type V CCGACTGCTCTGACGTAA 17 NED 100

Exfoliatin toxin A (Exfo. A) L25372 (2)

F_eta CCATATGGAGAGTATGAAGTCAAAGAAA 28 200

R_eta TGAAACACCGTTTTGATCTGGTT 23 200

P_eta TTAATGCTAAATCAACACCTGCACCAAATGGB 31 TET 100

Exfoliatin toxin B (Exfo. B) M13775 (3)

F_etb GAGGTAGGAAACTCTGGATCAGGTAT 26 200

R_etb TCTATTGAAAAACACTCCTATTGGAAGA 28 200

P_etb AGGTATTCACAGTGGTAAAGGCGGACAACA 30 JOE 100

Toxic shock syndrom toxin

(TSST-1) J02615 (4)

F_TSST-1 TCATCAGCTAACTCAAATACATGGATTA 28 200

R_TSST-1 TGTGGATCCGTCATTCATTGTT 22 200

P_TSST1 TCCAATAACCACCCGTTTTATCGCTTGA 28 FAM 100

A Minor groove binder probes with non-fluorescent quencher bound to the 3’-end (Applied Biosystems). B Taqman probes with a 3’-TAMRA quencher (Eurogentec, Seraing, Belgium) * Recently updated

References

1. Ito T, Ma XX, Takeuchi F, Okuma K, Yuzawa H, and Hiramatsu K. Novel type V staphylococcal cassette chromosome mec driven by a

novel cassette chromosome recombinase, ccrC. Antimicrob.Agents Chemother. 2004;48:2637-2651.

2. Sakurai S, Suzuki H, and Kondo I. DNA sequencing of the eta gene coding for staphylococcal exfoliative toxin serotype A.

J.Gen.Microbiol. 1988;134:711-717.

3. Jackson MP and Iandolo JJ. Sequence of the exfoliative toxin B gene of Staphylococcus aureus. J.Bacteriol. 1986;167:726-728.

4. Blomster-Hautamaa DA, Kreiswirth BN, Kornblum JS, Novick RP, and Schlievert PM. The nucleotide and partial amino acid sequence

of toxic shock syndrome toxin-1. J.Biol.Chem. 25-11-1986;261:15783-15786.