Supplementary Material · Web viewFigure 2S. Maximum likelihood (ML) tree based on bradyrhizobial...
11
Supplementary Material Table S1. List of primers used in this study Gene Primer name Sequence (5' to 3') References recA recAfX1 YGC MCT GCG TAT CGT YGA AGG ATC this study recAr3X1 CGA TCT TCA TSC GGA TCT GGT TGA TG this study dnaK TSdnaK4 GGC AAG GAG CCG CAY AAG G Stępkowski et al. (2007) TSdnaK2 GTA CAT GGC CTC GCC GAG CTT CA Stępkowski et al. (2003) glnII TSglnIIf AAG CTC GAG TAC ATC TGG CTC GAY GG Stępkowski et al. (2005) TSglnIIr SGA GCC GTT CCA GTC GGT RTC G Stępkowski et al. (2005) gyrB AMgyrBf GCA TGT ATA TCG GCG ACA C Stępkowski et al. (2012) AMgyrBr GTG AAG CAC AGY ACG TTC TC Stępkowski et al. (2012) rpoB rpoB-456F ATC GTY TCG CAG ATG CAC CG Vinuesa et al. (2008) rpoB-1364R TCG ATG TCG TCG ATY TCG CC Vinuesa et al. (2008) 16S rRNA fD1 AGA GTT TGA TCC TGG CTC AG Weisburg et al. (1991) rP2 ACG GCT ACC TTG TTA CGA CTT Weisburg et al. (1991) nifD TSnifDf1 CGV GGA GGT SCT SAA GGT CTA TCC Beukes et al. (2016) TSnifDr1 CCG AAG AAG TTG TAY TCG CAC CA this study nodA TSnodD1-1C CAG ATC NAG DCC BTT GAA RGC AT Stępkowski et al. (2005) TsnodB2N CTG TGR TTH GCR AYC TYR TGY CC Stępkowski et al. (2005) nodDDOA9 TAG GTC TAG GCC GCG RTA NCG CAT this study 1
Transcript of Supplementary Material · Web viewFigure 2S. Maximum likelihood (ML) tree based on bradyrhizobial...
Supplementary MaterialTable S1. List of primers used in this study
Gene Primer name Sequence (5' to 3') ReferencesrecA recAfX1 YGC MCT GCG TAT CGT YGA AGG ATC this study
recAr3X1 CGA TCT TCA TSC GGA TCT GGT TGA TG this studydnaK TSdnaK4 GGC AAG GAG CCG CAY AAG G Stępkowski et al. (2007)
TSdnaK2 GTA CAT GGC CTC GCC GAG CTT CA Stępkowski et al. (2003)glnII TSglnIIf AAG CTC GAG TAC ATC TGG CTC GAY GG Stępkowski et al. (2005)
TSglnIIr SGA GCC GTT CCA GTC GGT RTC G Stępkowski et al. (2005)gyrB AMgyrBf GCA TGT ATA TCG GCG ACA C Stępkowski et al. (2012)
AMgyrBr GTG AAG CAC AGY ACG TTC TC Stępkowski et al. (2012)rpoB rpoB-456F ATC GTY TCG CAG ATG CAC CG Vinuesa et al. (2008)
rpoB-1364R TCG ATG TCG TCG ATY TCG CC Vinuesa et al. (2008)16S rRNA fD1 AGA GTT TGA TCC TGG CTC AG Weisburg et al. (1991)
rP2 ACG GCT ACC TTG TTA CGA CTT Weisburg et al. (1991)nifD TSnifDf1 CGV GGA GGT SCT SAA GGT CTA TCC Beukes et al. (2016)
TSnifDr1 CCG AAG AAG TTG TAY TCG CAC CA this studynodA TSnodD1-1C CAG ATC NAG DCC BTT GAA RGC AT Stępkowski et al. (2005)
TsnodB2N CTG TGR TTH GCR AYC TYR TGY CC Stępkowski et al. (2005)nodDDOA9 TAG GTC TAG GCC GCG RTA NCG CAT this study
1
Figure 1S. Maximum likelihood (ML) tree based on bradyrhizobial dnaK gene sequences (707 bp). The significance of each branch is indicated by the bootstrap percentage calculated for 500 bootstraps. The bootstrap values greater than 60% are indicated at nodes. The number of sequences used in the construction of this phylogenetic tree is 322. Green square indicates our Bradyrhizobium isolates.
2
Figure 2S. Maximum likelihood (ML) tree based on bradyrhizobial glnII gene sequences (519 bp). The significance of each branch is indicated by the bootstrap percentage calculated for 500 bootstraps. The bootstrap values greater than 60% are indicated at nodes. The number of sequences used in the construction of this phylogenetic tree is 565. Green square indicates the heavy metals-tolerant Bradyrhizobium isolates.
3
Figure 3S. Maximum likelihood (ML) tree based on bradyrhizobial gyrB gene sequences (676 bp). The significance of each branch is indicated by the bootstrap percentage calculated for 500 bootstraps. The bootstrap values greater than 60% are indicated at nodes. The number of sequences used in the construction of this phylogenetic tree is 411. Green square indicates the heavy metals-tolerant Bradyrhizobium isolates.
4
Figure 4S. Maximum likelihood (ML) tree based on bradyrhizobial recA gene sequences (559 bp). The significance of each branch is indicated by the bootstrap percentage calculated for 500 bootstraps. The bootstrap values greater than 60% are indicated at nodes. The number of sequences used in the construction of this phylogenetic tree is 591. Green square indicates the heavy metals-tolerant Bradyrhizobium isolates.
5
Figure 5S. Maximum likelihood (ML) tree based on bradyrhizobial rpoB gene sequences (612 bp). The significance of each branch is indicated by the bootstrap percentage calculated for 500 bootstraps. The bootstrap values greater than 60% are indicated at nodes. The number of sequences used in the construction of this phylogenetic tree is 278. Green square indicates the heavy metals-tolerant Bradyrhizobium isolates.
6
