Supplementary Figure S2, Lecoeur et al.
1
Supplementary Figure S2, Lecoeur et al. Supplementary Figure S2. Effect of ruthenium red on Tat-induced swelling. Mitochondria were isolated from mouse heart and liver by differential centrifugation (according to Ref. 38), exposed or not to 1 µM ruthenium red (R. Red) for 5 min and then treated with full-length Tat[1-86] 0.6 M and mitochondrial swelling (measured as 90° light scattering at 545 nm) was monitored for 30 min. Percentages of mitochondrial swelling was calculated as described under material & methods. Positive control for swelling was defined by the addition of 50 µM CaCl 2 . Histograms represent mean values (+/- standard deviations) of 3 independent experiments. . 0 20 40 60 80 100 120 NT - swelling (%) Tat R. Red Ca 2+ - R. Red 0 20 40 60 80 100 120 NT - swelling (%) Tat R. Red Ca 2+ - R. Red Liver mitochondria Heart mitochondria
-
Upload
reed-lyons -
Category
Documents
-
view
18 -
download
1
description
120. 120. 100. 100. 80. 80. swelling (%). swelling (%). 60. 60. 40. 40. 20. 20. 0. 0. -. -. -. -. NT. NT. R. Red. R. Red. R. Red. R. Red. Ca 2+. Tat. Ca 2+. Tat. Heart mitochondria. Liver mitochondria. - PowerPoint PPT Presentation
Transcript of Supplementary Figure S2, Lecoeur et al.
Supplementary Figure S2, Lecoeur et al.
Supplementary Figure S2. Effect of ruthenium red on Tat-induced swelling. Mitochondria were isolated from mouse heart and liver by differential centrifugation (according to Ref. 38), exposed or not to 1 µM ruthenium red (R. Red) for 5 min and then treated with full-length Tat[1-86] 0.6 M and mitochondrial swelling (measured as 90° light scattering at 545 nm) was monitored for 30 min. Percentages of mitochondrial swelling was calculated as described under material & methods. Positive control for swelling was defined by the addition of 50 µM CaCl2. Histograms represent mean values (+/- standard deviations) of 3 independent experiments. .
0
20
40
60
80
100
120
NT -
swel
ling
(%
)
Tat
R.
Red
Ca2+
-
R.
Red
0
20
40
60
80
100
120
NT -
swel
ling
(%
)
Tat
R.
Red
Ca2+
-
R.
Red
Liver mitochondria Heart mitochondria
