Supplemental Figure 2 - Genes &...
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siRNA -24 hrs Thymidine Release siRNA Thymidine 0 Release -39 hrs -20 hrs -15 hrs -44 hrs 9 hrs Mitosis ± Reversine 0.5 μM or AZ3146 2 μM 15 hrs Wash-out 15 hrs 5 hrs 4 hrs 5 hrs 15 hrs Bub1 RNAi Mad2 RNAi Rev 0.5μM AZ 2μM Ctrl 100 KDa 37 20 15 WB 33 hrs 0 Drug Treatment Bub1 RNAi Mad2 RNAi Rev 0.5μM AZ 2μM Ctrl WB 57 hrs 0 Drug Treatment LC3-I LC3-II Mad2 Actin Bub1 B A 100 KDa 37 siRNA Ctrl Bub1 Mad2 20 15 LC3-I LC3-II Mad2 Actin Bub1 Ctrl Bub1 Mad2 + ZM447439 C D IMR-90 15 KDa 37 Actin Ctrl Rev LC3-I LC3-II Lymphoblastoid 15 KDa 100 Vinculin Ctrl Rev LC3-I LC3-II LC3-I LC3-II Actin Ctrl Rev AZ BJ-hTERT 15 KDa 37 E F Santaguida_Supplemental Figure 3
Transcript of Supplemental Figure 2 - Genes &...
siRNA
-24 hrs
Thymidine Release siRNA Thymidine
0
Release
-39 hrs -20 hrs -15 hrs-44 hrs 9 hrs
Mitosis
± Reversine 0.5 µMor AZ3146 2 µM
15 hrs
Wash-out15 hrs5 hrs 4 hrs 5 hrs 15 hrs
Bub1RNAi
Mad2RNAi
Rev0.5µM
AZ2µMCtrl
100 KDa
37
20
15
WB
33 hrs0
Drug Treatment
Bub1RNAi
Mad2RNAi
Rev0.5µM
AZ2µMCtrl
WB
57 hrs0
Drug Treatment
LC3-ILC3-II
Mad2
Actin
Bub1
B
A
100 KDa
37
siRNA Ctrl Bub1 Mad2
20
15 LC3-ILC3-II
Mad2
Actin
Bub1Ctrl Bub1 Mad2+ ZM447439
C
DIMR-90
15 KDa
37 Actin
Ctrl RevLC3-ILC3-II
Lymphoblastoid
15 KDa
100 Vinculin
Ctrl RevLC3-ILC3-II
LC3-ILC3-II
Actin
Ctrl Rev AZBJ-hTERT
15 KDa
37
E
F
Santaguida_Supplemental Figure 3