Suppl. figure 1 · 2007-11-20 · Suppl. Fig. 1. Expression of miR-21 in cell lines. The amount of...
Transcript of Suppl. figure 1 · 2007-11-20 · Suppl. Fig. 1. Expression of miR-21 in cell lines. The amount of...
Suppl. figure 1
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HEKU2O
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Suppl. Fig. 1. Expression of miR-21 in cell lines. The amount of miR-21 and U6 RNA was quantified using Taqman MicroRNA Assays (Applied Biosystems). Shown are the relative normalized miR-21 amounts (HEK=1). Suppl. Fig. 2. Efficient inhibition of miR-21 by the LNA inhibitor. Firefly luciferase reporter assay with pmiR-21-luc containing perfect compementarity to the mature miR-21 sequence. pmiR-21-luc was cotransfected into MCF-7 cells along with a Renilla luciferase transfection control plasmid, either alone or together with a miR-21 inhibitor or a scrambled control. Shown are relative luciferase values normalized to transfections with the reporter alone. Suppl. Fig. 3. Western blot for PTEN using lysates from MCF-7 cells transfected with miR-21 or lin-4 precursors or inhibitory miR-21 or scrambled LNAs. The bands were quantified relative to the appropriate Vinculin loading controls using a LAS-3000 imager (Fuji) and the relative quantifications are shown. Suppl. Fig. 4. Western blot for p53 using lysates from MCF-7 cells transfected with miR-21 or lin-4 precursors or inhibitory miR-21 or scrambled LNAs. The bands were quantified relative to the appropriate Vinculin loading controls using a LAS-3000 imager (Fuji) and the relative quantifications are shown. Suppl. Fig. 5. Knockdown of PDCD4 does not significantly affect cellular proliferation. O.D. values from a MTT growth assay in MCF-7 cells treated with PDCD4 or control siRNAs and transfected with inhibitors against miR-21 or a scrambled control. Data are shown as the mean ± SD of 3 replicates and are representative of 3 independent experiments.